functional anatomy of the cns

... respectively, of that of HPRT. This low activity could be explained by the fact that PRTFDC1 has a Gly in the position of the proposed catalytic Asp of HPRT. In PRTFDC1, a water molecule at the position of ... strength [20]. In the present study, we report the structural and functional characterization of the human HPRT homolog PRTFDC1. To shed light on the potential function of PRTFDC1, the protein was ... interacts with both O6 and N7 (Fig. 1C). The 2¢OH of the ribose is hydro- gen bonded to the main chain and the side chain of Asp142 (Fig. 1C). Both hydroxyl groups of the ribose are involved in coordinating...

Ngày tải lên: 15/02/2014, 01:20

... analyses of the predicted amino acid sequence showed that the C-terminal half of the RepA of the pIT3 plasmid is sequence-similar to the helicases of the phage-encoded superfamily III pro- teins. The ... probably comparable with the two-metal-ion mechanism of both RNA and DNA synthesis [17]. In a previous study, we reported the findings of an analysis of the complete sequence of the cryptic plas- mid ... subunit of archaeal primases and the active site of the X family of polymerases [23]. Indeed, unlike the pRN1 prim–pol protein whose motif is DXE D, the Rep245 protein, the X family of DNA polymerases and...

Ngày tải lên: 18/02/2014, 18:20

... connected to the DEC1 E-boxes (Fig. 2), while deletion of the Orange domain ( DEC1DOr- ange) had no effect on the suppressive activity, indicating the importance of t he bHLH region of DEC1. The HXXXXXXXR ... conclude that the basic region, p artic- ularly the conserved A rg65, but not His57, is essential for the suppressive activity of DEC1. Determination of the binding domain of DEC1 to BMAL1 The interaction ... that the C-terminal region, including the PAS-B domain of BMAL1, is required for the binding to DEC1, Fig. 4. Identification of the domain of D EC1 that interacts with BMAL 1. Interactions of various...

Ngày tải lên: 19/02/2014, 16:20

... Binding of CCE1 results in the complete unpairing of the four base-pairs in the centre of the junctions with a lesser disruption of the next base-pairs [19], which is reminiscent of the structure of ... 4 at the junction crossover – and the unpairing of four base-pairs in the centre of the junctions [19]. Detailed understanding of the functional role of these interactions would require the crystal ... manipulating the global conformation of the junction, then the deletion of this motif would have an even more serious effect on Ydc2 binding in the presence of divalent cations. We tested the effect of...

Ngày tải lên: 20/02/2014, 11:20

... PrP. Production of deletion mutants of PrP On the basis of the results with antibodies, a series of PrP mutants were made to assess whether deletions of certain domains of the protein decrease the SOD-like ... [31]. The relevance of the findings presented here are therefore quite important in determining the changes that PrP undergoes during the course of prion disease and the possible role of the loss of its ... syndrome [6]. All of these disease are linked together because of the deposition of an abnormal, protease-resistant isoform of the prion protein in brains of individuals with these diseases. This...

Ngày tải lên: 21/02/2014, 00:20

... and then the energy minimization of the model was iterated 300 cycles using the conjugate gradient methods with the program DISCOVER _3 in INSIGHT II . To construct the dimeric form of LPL, the model ... as the high- affinity peak of wild-type LPL. The LPL in the eluate was detected by Western blot with LPL polyclonal antibody 7640. The fraction number of the high-affinity peak was the same for the ... mutant, the two LPL peaks both eluted at lower salt concentrations than those of wild-type LPL. The R405A mutant exhibited an earlier elution of the high-affinity peak, whereas the elution of the...

Ngày tải lên: 21/02/2014, 15:20

... either component of the import machinery significantly decreased the amount of perox- ins of the importomer pulled down by the AAA perox- ins, whereas the amount of components of the AAA complex ... a control. The corresponding solubilisates (right panel) were analyzed for the presence of Pex1p and Pex6p as indicated. The defici- ency of either protein of the AAA peroxin couple in the absence of ... cytosol. The specifity of the precipitation is confirmed by the lack of detection of the abundant proteins Fbp1p and thiolase in the eluates (data not shown). Figure 1B shows a compar- ison of the cytosolic...

Ngày tải lên: 07/03/2014, 12:20

... comprising the N-terminal fragment and the first AAA-cassette of PEX6 (PEX6N + D1–GAL4-BD). Neither of the cas- settes alone nor the two AAA-cassettes together or the N-terminal part together with the ... reflects the different sizes of the fusion proteins. Immunological analysis revealed the presence of Pex6p in the precipitate of full-length Pex1p–ProtA and also the presence of Pex1p in the full-length ... activation of the reporter gene and thus is suffi- cient to maintain the interaction with Pex1p. These findings demonstrate the importance of the first AAA- cassette together with the N-terminal region of...

Ngày tải lên: 07/03/2014, 16:20

... glycosaminoglycans [37]. As the myc-his tag of the protein was present at the C-terminus of the receptor which is located on the inside of the cell, we then determined if signal transduction by the protein was ... respectively. Purification of CCR5-myc-his For the purification of CCR5-myc-his we tested two different methods based on the presence of the six histidines at the C-terminus of the protein. The first involved immobilized ... reconstitution; liposome. Reconstitution of membrane proteins allows the study of their behaviour in a membrane in the absence of other proteins, and the manipulation of their concentration and environment....

Ngày tải lên: 08/03/2014, 09:20

... analysis of the 5 ¢ end of the xMGP promoter reveale d a minimal activating ele- ment in the s equence from )70 to )36 bp. Synthetic reporter constructs containing three c opies of the d efined ... identified 5¢ end of the xMGP cDNA [4]. The lower group of bands, identified as site ÔBÕ in Fig. 4, probably corresponds to a premature arrest of the reverse transcriptase due to the presence of an inverted ... copies of the )70/)36 regulatory sequence led to a nearly 400-fold induction of reporter gene e xpression, further c onfirming the i mportance of the regulatory element for xMGP gene expression. These...

Ngày tải lên: 08/03/2014, 10:20

... decrease in the total intensity of the stained bands, confirming the h igh sensitivity of the HPLC method (inset A of Fig. 4). Nevertheless, Fig. 4 (inset B) shows the time course of spectroscopic ... cyanobacteria showed the disappear- ance of the h eaviest sucrose band 5 (inset of Fig. 7B). DISCUSSION In a recent paper the complete resolution of the protein components of phycobilisomes from the cyanobacterium Synechocystis ... certain cases they have been shown to improve the energy migration process [7]. As some of the linkers mediate assembly of the biliproteins, they produce changes in the spectra of biliproteins,...

Ngày tải lên: 08/03/2014, 16:20

... thermostability of the enzyme (Table S2), and thermal unfolding of the wild-type and both the F193A and W373K mutants was found to be irreversible (Fig. S3). The propensity of the wild-type and each of the single ... introducing features of the consensus motif into the Zm-p60.1 scaffold, an analysis of structural and catalytic prop- erties of the mutants, and simulations of the sub- strate–enzyme interactions of the wild-type ... cleavage and the roles of the catalytic pair, our knowledge of the molecular determinants of aglycone specificity in b-glucosidases remains limited. Elucidation of the aglycone specificity of b-glucosidases is...

Ngày tải lên: 16/03/2014, 04:20

... of the PDI [42], and t hat this site is far away from the redox active sites in the sequ ence. Furthermore, the measurements of the rates of PDI-catalysed refolding of scrambled RNase A, in the ... (225 m M )9:1 (25 m M ). At the end of the incubation, 40 lL of the mixture was added to each of the five solutions; these were then left to react on ice for 5 min. The reaction mixtures w ere analysed ... of the active site in the N-unit. As regards the oxidative activity, the two units also display differences in their functional properties, with the site at the C-termus always predominant, the...

Ngày tải lên: 16/03/2014, 16:20

... amplitude of the fast phase of the l atter represen ted up to 40% of the total amplitude, with a rate constant (k obs ) independent of Pc concentration (data not shown). From the k obs values of this fast ... interactions with both PSI and Cyt f, but the solvent-exposed edge of His87, located in the hydrophobic patch at the so-called Ônorthern sideÕ of the protein, constitutes the electron transfer pathway to ... P c/fern PSI s ystem are of the same order of magnitude as those observed for the spinach Pc/PSI system [15], the distance and/or orientation o f the redox centers in the fern Pc/PSI complex seems...

Ngày tải lên: 16/03/2014, 16:20

... determined pI of 6.1 and the theoretically deduced pI value of 6.22 was found. A theoretical prediction of the secondary structural organization of Ag-NPA-1 performed on the basis of the amino acid ... cellular distribution of the protein. The binding affinities are characterized by changes in the fluorescence of the single Trp chromophore that is used as a marker of the protein conformation and of the thiol ... 7B). These observations suggest that Ag-NPA-1 is localized mainly in cells of the inner hypodermis and the epithe- lium of the oviduct as well as extracellularly in the pseudocoelomic cavity of the...

Ngày tải lên: 16/03/2014, 18:20

Ngày tải lên: 16/03/2014, 18:20

Ngày tải lên: 16/03/2014, 23:20

Ngày tải lên: 17/03/2014, 10:20