functional anatomy of the cns
Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... respectively, of that of HPRT. This low activitycould be explained by the fact that PRTFDC1 has a Gly in the position of the proposed catalytic Asp of HPRT. In PRTFDC1, a water molecule at the position of ... strength [20].In the present study, we report the structural and functional characterization of the human HPRThomolog PRTFDC1. To shed light on the potentialfunction of PRTFDC1, the protein was ... interacts with bothO6 and N7 (Fig. 1C). The 2¢OH of the ribose is hydro-gen bonded to the main chain and the side chain of Asp142 (Fig. 1C). Both hydroxyl groups of the riboseare involved in coordinating...
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Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc
... analyses of the predictedamino acid sequence showed that the C-terminal half of the RepA of the pIT3 plasmid is sequence-similar to the helicases of the phage-encoded superfamily III pro-teins. The ... probably comparablewith the two-metal-ion mechanism of both RNA andDNA synthesis [17].In a previous study, we reported the findings of ananalysis of the complete sequence of the cryptic plas-mid ... subunit of archaeal primases and the activesite of the X family of polymerases [23]. Indeed, unlike the pRN1 prim–pol protein whose motif is DXE D, the Rep245 protein, the X family of DNA polymerasesand...
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Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt
... connected to the DEC1 E-boxes(Fig. 2), while deletion of the Orange domain ( DEC1DOr-ange) had no effect on the suppressive activity, indicating the importance of t he bHLH region of DEC1. The HXXXXXXXR ... conclude that the basic region, p artic-ularly the conserved A rg65, but not His57, is essential for the suppressive activity of DEC1.Determination of the binding domain of DEC1 to BMAL1 The interaction ... that the C-terminal region, including the PAS-Bdomain of BMAL1, is required for the binding to DEC1,Fig. 4. Identification of the domain of D EC1that interacts with BMAL 1. Interactions of various...
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Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx
... Binding of CCE1 results in the complete unpairing of the four base-pairs in the centre of the junctions with a lesser disruption of the next base-pairs[19], which is reminiscent of the structure of ... 4 at the junction crossover – and the unpairing of four base-pairs in the centre of the junctions [19]. Detailedunderstanding of the functional role of these interactionswould require the crystal ... manipulating the global conformation of the junction, then the deletion of this motif would have aneven more serious effect on Ydc2 binding in the presence of divalent cations. We tested the effect of...
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Tài liệu Báo cáo khoa học: Mapping the functional domain of the prion protein docx
... PrP.Production of deletion mutants of PrPOn the basis of the results with antibodies, a series of PrPmutants were made to assess whether deletions of certaindomains of the protein decrease the SOD-like ... [31]. The relevance of the findings presented here are therefore quite important indetermining the changes that PrP undergoes during the course of prion disease and the possible role of the loss of its ... syndrome[6]. All of these disease are linked together because of the deposition of an abnormal, protease-resistant isoform of the prion protein in brains of individuals with these diseases.This...
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Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx
... and then the energyminimization of the model was iterated 300 cycles using the conjugate gradient methods with the programDISCOVER_3inINSIGHT II. To construct the dimeric form of LPL, the model ... as the high-affinity peak of wild-type LPL. The LPL in the eluate wasdetected by Western blot with LPL polyclonal antibody7640. The fraction number of the high-affinity peak was the same for the ... mutant, the two LPL peaks both eluted at lower salt concentrationsthan those of wild-type LPL. The R405A mutant exhibitedan earlier elution of the high-affinity peak, whereas the elution of the...
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Báo cáo khoa học: Functional association of the AAA complex and the peroxisomal importomer potx
... either component of the importmachinery significantly decreased the amount of perox-ins of the importomer pulled down by the AAA perox-ins, whereas the amount of components of the AAAcomplex ... acontrol. The corresponding solubilisates(right panel) were analyzed for the presence of Pex1p and Pex6p as indicated. The defici-ency of either protein of the AAA peroxincouple in the absence of ... cytosol. The specifity of the precipitation is confirmed by the lack of detection of the abundant proteins Fbp1p and thiolase in the eluates (data not shown). Figure 1B shows a compar-ison of the cytosolic...
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Báo cáo khoa học: Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p pptx
... comprising the N-terminal fragment and the first AAA-cassette of PEX6 (PEX6N + D1–GAL4-BD). Neither of the cas-settes alone nor the two AAA-cassettes together or the N-terminal part together with the ... reflects the differentsizes of the fusion proteins. Immunological analysisrevealed the presence of Pex6p in the precipitate of full-length Pex1p–ProtA and also the presence of Pex1p in the full-length ... activation of the reporter gene and thus is suffi-cient to maintain the interaction with Pex1p. Thesefindings demonstrate the importance of the first AAA-cassette together with the N-terminal region of...
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Báo cáo Y học: Functional reconstitution of the HIV receptors CCR5 and CD4 in liposomes pot
... glycosaminoglycans [37].As the myc-his tag of the protein was present at the C-terminus of the receptor which is located on the inside of the cell, we then determined if signal transduction by the protein was ... respectively.Purification of CCR5-myc-hisFor the purification of CCR5-myc-his we tested twodifferent methods based on the presence of the six histidinesat the C-terminus of the protein. The first involvedimmobilized ... reconstitution; liposome.Reconstitution of membrane proteins allows the study of their behaviour in a membrane in the absence of otherproteins, and the manipulation of their concentration andenvironment....
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Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identifies a calcium sensitive region required for basal activity doc
... analysis of the 5 ¢ end of the xMGP promoter reveale d a minimal activating ele-ment in the s equence from )70 to )36 bp. Synthetic reporterconstructs containing three c opies of the d efined ... identified 5¢ end of the xMGP cDNA [4]. The lower group of bands, identified as site ÔBÕ in Fig. 4,probably corresponds to a premature arrest of the reversetranscriptase due to the presence of an inverted ... copies of the )70/)36regulatory sequence led to a nearly 400-fold induction of reporter gene e xpression, further c onfirming the i mportance of the regulatory element for xMGP gene expression.These...
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Báo cáo Y học: Functional studies of the Synechocystis phycobilisomes organization by high performance liquid chromatography on line with a mass spectrometer docx
... decrease in the total intensity of the stained bands, confirming the h igh sensitivity of the HPLC method (inset A of Fig. 4). Nevertheless, Fig. 4(inset B) shows the time course of spectroscopic ... cyanobacteria showed the disappear-ance of the h eaviest sucrose band 5 (inset of Fig. 7B).DISCUSSIONIn a recent paper the complete resolution of the proteincomponents of phycobilisomes from the cyanobacteriumSynechocystis ... certain cases they have beenshown to improve the energy migration process [7]. As some of the linkers mediate assembly of the biliproteins, theyproduce changes in the spectra of biliproteins,...
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Báo cáo khoa học: Functional analysis of the aglycone-binding site of the maize b-glucosidase Zm-p60.1 pot
... thermostability of the enzyme (Table S2), and thermal unfolding of the wild-type and both the F193A and W373K mutantswas found to be irreversible (Fig. S3). The propensity of the wild-type and each of the single ... introducingfeatures of the consensus motif into the Zm-p60.1scaffold, an analysis of structural and catalytic prop-erties of the mutants, and simulations of the sub-strate–enzyme interactions of the wild-type ... cleavage and the roles of the catalyticpair, our knowledge of the molecular determinants of aglycone specificity in b-glucosidases remains limited.Elucidation of the aglycone specificity of b-glucosidasesis...
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Báo cáo khóa học: Functional properties of the protein disulfide oxidoreductase from the archaeon Pyrococcus furiosus A member of a novel protein family related to protein disulfide-isomerase doc
... of the PDI [42], and t hat this site is far away from the redox active sites in the sequ ence. Furthermore, the measurements of the rates of PDI-catalysed refolding of scrambled RNase A, in the ... (225 mM)9:1(25 mM). At the end of the incubation, 40 lL of the mixturewas added to each of the five solutions; these were then leftto react on ice for 5 min. The reaction mixtures w ereanalysed ... of the active site in the N-unit. As regards the oxidative activity, the two units alsodisplay differences in their functional properties, with the site at the C-termus always predominant, the...
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Báo cáo khóa học: Functional characterization of the evolutionarily divergent fern plastocyanin potx
... amplitude of the fast phase of the l atter represen ted up to 40% of the totalamplitude, with a rate constant (kobs) independent of Pcconcentration (data not shown). From the kobsvalues of thisfast ... interactions withboth PSI and Cyt f, but the solvent-exposed edge of His87,located in the hydrophobic patch at the so-called ÔnorthernsideÕ of the protein, constitutes the electron transfer pathwayto ... P c/fern PSI s ystem are of the sameorder of magnitude as those observed for the spinach Pc/PSIsystem [15], the distance and/or orientation o f the redoxcenters in the fern Pc/PSI complex seems...
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Báo cáo khoa học: Conformational and functional analysis of the lipid binding protein Ag-NPA-1 from the parasitic nematode Ascaridia galli potx
... determined pI of 6.1 and the theoretically deduced pI value of 6.22 wasfound.A theoretical prediction of the secondary structuralorganization of Ag-NPA-1 performed on the basis of the amino acid ... cellulardistribution of the protein. The binding affinities arecharacterized by changes in the fluorescence of the singleTrp chromophore that is used as a marker of the proteinconformation and of the thiol ... 7B). Theseobservations suggest that Ag-NPA-1 is localizedmainly in cells of the inner hypodermis and the epithe-lium of the oviduct as well as extracellularly in the pseudocoelomic cavity of the...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008