functional analysis of human islets of langerhans maintained in culture
Báo cáo khoa học: Functional analysis of disease-causing mutations in human galactokinase potx
Ngày tải lên :
08/03/2014, 02:20
... group of GHMP kinases in which proton transfer does not play a major role in the rate determining step of catalysis That five of the 10 disease-causing mutations resulted in insoluble protein in ... quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 26 Wang, W & Malcolm, B.A (1999) Two-stage PCR protocol allowing introduction of ... at some point in the reaction Modest changes in Km,ATP are seen in H44Y and R68C That H44 in uences the binding of both substrates suggests that the binding sites are probably close in space R68...
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Báo cáo khoa học: Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation pptx
Ngày tải lên :
16/03/2014, 10:20
... Localization of the ET-1-binding site In order to determine the ETB region that is essential for binding of ET-1, a series of eight plasmids coding for terminally truncated ETB fragments and containing ... N-terminal domain and C-terminal domain, which are both often involved either in ligand binding or G-protein coupling Owing to their key role in signal transduction in eukaryotic cells, GPCRs ... by using : Langmuir models The determined kd was used for calculation of ka, and the binding constants KD were determined from kd ⁄ ka We determined the binding constant KD for binding of ETBcHx...
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Báo cáo khoa học: Functional analysis of disease-causing mutations in human UDP-galactose 4-epimerase pot
Ngày tải lên :
23/03/2014, 11:20
... during purification Kinetic analysis of disease-causing mutations The steady-state kinetic parameters of each of the nine disease-causing mutant proteins were determined (Table 1) In general, little ... quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 Wang W & Malcolm BA (1999) Two-stage PCR protocol allowing introduction of multiple ... of disease Some of the mutants have only small changes in the kinetic parameters In the case of N34S and D103G, disease-causation may be explained by a combination of kinetic impairment and increased...
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Báo cáo y học: " Genome-wide functional analysis of human 5’ untranslated region introns" ppsx
Ngày tải lên :
09/08/2014, 20:21
... distribution of the log10 of the total number of intronic nucleotides in the 5’UTR (b) Distribution of the number of introns in the 5’UTR The log10 of number of transcripts that have a given number of introns ... tyrosine kinase (CSK), janus kinases (JAK), spleen tyrosine kinase (SYK), tec protein tyrosine kinase (TEC), and Bruton agammaglobulinemia tyrosine kinase (BTK) among others To gain insight into the ... distribution of these introns in different functional categories of genes Results Characterization of a set of genes with 5’UTR introns To investigate the functional properties of human 5UIs,...
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Báo cáo y học: " Functional analysis of human T lymphotropic virus type 2 Tax proteins" pptx
Ngày tải lên :
13/08/2014, 09:21
... promoters indicating that this domain is not as critical in Tax and 2B for activity as it is in Mo However the insertion of the amino acid arginine instead of the hydrophobic amino acid cysteine at ... Location of mutations found in Tax 2A proteins Location of mutations found in Tax 2A proteins (A) Schematic representation of functional domains in Tax 2A Mo These regions include CREB binding domains ... identified functional domains in Tax which are required for NFkB and LTR activation These regions include activation domains at the amino and carboxy termini, a CREB binding domain and zinc binding...
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Báo cáo y học: " Functional analysis of human and chimpanzee promoters" docx
Ngày tải lên :
14/08/2014, 14:21
... values indicate higher expression in humans (that is, a value of means twofold higher expression in humans); ¶average signal log2 ratio for the promoter activity in cell lines; *, ** and *** indicate ... go in the same direction in at least one of the cell lines as they in the tissues, whereas they go in the opposite direction for four genes (CGI-51, SH3BGR, UNG, TERF) in one of the two cell lines ... differences in promoter activity [27] into a human neuroblastoma cell line and a human cervical carcinoma cell line, respectively Experiments were performed in triplicate and the activities of the...
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Functional analysis of human nima related kinase 8 (nek8)
Ngày tải lên :
11/09/2015, 10:03
... domain of importinα helps it to interact with importinβ Importinβ has a distinct domain binding with Ran GTPase, and two binding sites for the hydrophobic FG repeats of certain Nups allowing ... several subsets: serine/threonine-specific protein kinases, tyrosine-specific protein kinases and serine/threonine-tyrosine mixed kinases, etc Among them, serine/threonine kinases are the most ... to binding with the nucleotide-free form of Rac1, indicating that TD-60 may be a Rac1-GEF HERC proteins have a HECT domain and proteins containing this domain are suggested to be involved in transfering...
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Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt
Ngày tải lên :
19/02/2014, 16:20
... addition, the N-terminal region of DEC1, including the bHLH domain, interacted with the C-terminal region of BMAL1 in a mammalian two-hybrid assay Accordingly, a Ó FEBS 2004 Interaction of DEC1 with ... Western blot analysis (Fig 5) These results indicate that the bHLH region, including His57 and Arg65, is responsible for the E-box binding Determination of the region in BMAL1 for binding to DEC1 ... (BMAL1:112–626) did not affect the binding of BMAL1 to DEC1; deletion of 235 amino acids (BMAL1:236–626) slightly diminished the binding, although a strong binding ability of BMAL1:236–626 with DEC1 still...
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Tài liệu Báo cáo Y học: Functional analysis of DM64, an antimyotoxic protein with immunoglobulin-like structure from Didelphis marsupialis serum pdf
Ngày tải lên :
21/02/2014, 01:21
... (clear boxed in Fig 4) A gap of four amino acids beginning after residue 242 of DM64 is also present in human a1B-glycoprotein Such gap was not found on the third domain of DM43 Inhibitory properties ... activities of myotoxin I, even when a twofold molar excess of the inhibitor was used (not shown) Myotoxin II was not tested, since it is devoid of these activities DM64 was also ineffective in the inhibition ... phenylalanine and tyrosine typical of the Ig-fold are bold in each domain DM43 has three Ig-like domains [24], while a1B-glycoprotein is a five-Ig-like domain protein of 63 kDa [41] Considering the...
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Tài liệu Báo cáo Y học: Functional analysis of a small heat shock/a-crystallin protein from Artemia franciscana docx
Ngày tải lên :
22/02/2014, 04:20
... and incubated with shaking at 37 °C until the D600 reached 0.6–1.0 Cultures were stored at °C overnight before incubation with shaking in 50 mL of fresh LB medium containing 50 lgÁmL)1 ampicillin ... amounts of p26 in lysates of transformed E coli were determined by incubating Western blots with anti-p26 Ig, taking care to ensure that density measurements were within the linear range of film ... was small In contrast, loss of 42 residues from the N-terminus of a rice small heat shock/a-crystallin protein increased oligomer size [23] Deleting the last 16 C-terminal residues of C elegans...
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Báo cáo khoa học: Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus pot
Ngày tải lên :
06/03/2014, 22:21
... cured of prophages; 11 bp deletion in rsbU Mutant strain of 8325-4 defective in protein A Strain 8325-4 with repaired defect in rsbU Mutant strain of SH1000 defective in protein A Mutant strain of ... including expression of cell adhesion molecules and secretion of chemokines and cytokines [34] Fn-binding proteins also mediate activation of human platelets via fibrinogen and Fn bridges to integrin ... antibodies against FnBRs of FnBPB A panel of mouse mAbs was produced against the recombinant repetitive region of FnBPB Analysis of mAbs binding to the recombinant FnBR indicated the generation of two...
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Báo cáo khoa học: Functional analysis of mutations in UDP-galactose-4epimerase (GALE) associated with galactosemia in Korean patients using mammalian GALE-null cells pdf
Ngày tải lên :
07/03/2014, 00:20
... galactose was determined by measuring BrdU incorporation The level of BrdU incorporation into each cell line cultured in the absence of galactose was set to 100% and the corresponding results from ... expressing mutant GALE was increased to reach a detectable level of protein Tubulin serves as a loading control The relative amount of mutant protein was calculated based on the ratio of the band intensities ... accordingly Relative protein abundance (RPA) was calculated based on the ratio of intensity of the myc–GALE to tubulin bands in the western blot and normalized by setting the amount of GALE in ldlD(myc–GALE)...
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Báo cáo khoa học: Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p pptx
Ngày tải lên :
07/03/2014, 16:20
... that a mutation in the beginning of Pex1pD2 (G843D in human, corresponding to G700 in yeast) attenuates the interaction of the two AAA-peroxins [23] To identify the Pex6p binding site for Pex1p, ... contributes to the binding of the two proteins Truncation of either protein, however, did result in a significant decrease in the coimmunoprecipitation of the binding partner These data might indicate that ... nucleotide-binding, while the WalkerB motif is required for hydrolysis of the nucleotide To investigate the in uence of the binding and ⁄ or hydrolysis of ATP on the interaction of the AAA-peroxins,...
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Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identifies a calcium sensitive region required for basal activity doc
Ngày tải lên :
08/03/2014, 10:20
... Mapping the transcription start site of the xMGP gene Fig Sites of intron insertions within the amino-acid sequence of Xenopus, human and mouse MGPs Conserved sites of intron insertions in mammalian ... within the MGP coding sequence (Fig 2) Analysis of the phase of each of the xMGP introns located within the coding region revealed that introns and are of phase I while intron is of phase II [29] ... suggested the presence of specic binding sites for nuclear factors involved in the regulation of MGP gene transcription in the )70/)36 region Binding of A6 nuclear protein(s) to this region was...
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Báo cáo Y học: Monoterpene biosynthesis in lemon (Citrus limon) cDNA isolation and functional analysis of four monoterpene synthases pot
Ngày tải lên :
08/03/2014, 23:20
... %Sj) of (–)-a-pinene The sabinene side product of Cl(–)bPINS was determined to be 74% e.e of (–)-sabinene (Table 2) ClcTS produced (–)-a-pinene as a side product with an e.e of 24%, but (+)-b-pinene ... 2,3-diethyl-6-tertbutyl-dimethyl-b-cyclodextrin/PS086 (25 m · 0.25 mm internal diameter; film thickness ¼ 0.15 lm) was maintained at 60 °C (15 min) then programmed to 200 °C at 0.5 °CÆmin)1 with He gas flow at mLÆmin)1 The compounds of interest ... in the present paper Only the origin of the trace amounts of linalool, a-terpineol and (E)-b-ocimene that are also present in the lemon extract remain unexplained, as they are not a product of...
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Báo cáo khoa học: Functional analysis of the aglycone-binding site of the maize b-glucosidase Zm-p60.1 pot
Ngày tải lên :
16/03/2014, 04:20
... moieties of these substances differs distinctly, prompted us to initiate a bioinformatic analysis of plant b-glucosidases to obtain insights into the evolution of the molecular sites involved in the ... results of CD spectroscopy (Fig 4) The high degree of structural alteration might indicate an involvement of F200 in folding of Zm-p60.1 Interestingly, an F200L mutation was shown to cause an increase ... Accumulation of the four mutations in a single molecule of the quadruple P2 mutant resulted in the polypeptide chain folding into a distinct structure characterized by an inversed ratio of a helices...
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Báo cáo khoa học: Conformational and functional analysis of the lipid binding protein Ag-NPA-1 from the parasitic nematode Ascaridia galli potx
Ngày tải lên :
16/03/2014, 18:20
... residue, buried in the interior of the protein molecule, to the ionone ring of retinol would be expected Interestingly, binding of retinol caused an increase of the emission of the single Trp residue, ... recorded In addition to the protein peaks, they also contained IAEDANS or IANBD bands, indicative of covalent binding of the dyes [19] Binding activities of Ag-NPA-1 Binding of fatty acids, retinol ... (ov) in A suum (D) Section of the body wall of a female A galli showing an intense labeling of the inner hypodermis (ihy, arrow) (E) Staining of the labyrinth of the lateral chord (lc, arrow) in...
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Báo cáo khoa học: Functional analysis of two divalent metal-dependent regulatory genes dmdR1 and dmdR2 in Streptomyces coelicolor and proteome changes in deletion mutants ppt
Ngày tải lên :
16/03/2014, 18:20
... shows an increased level in the dmdR1 mutant, is a DNA-binding protein with domains typical of the ferritin superfamily This protein might be involved in a cascade of iron regulation in response ... Large increase Gas-vesicle protein sco6501 P3 Medium increase P4 Small increase P5 Medium increase DNA-binding protein of the ferritin family sco0596 P2 Medium increase Phosphatidylethanolaminebinding ... role of domain on DNAÆprotein interaction and of domain in the protein dimerization and metal binding (see the Discussion) There are important differences between DmdR1 and DmdR2 proteins in a...
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Báo cáo khoa học: A novel mass spectrometric approach to the analysis of hormonal peptides in extracts of mouse pancreatic islets ppt
Ngày tải lên :
17/03/2014, 10:20
... of the many compounds of the pancreatic islet extract (Fig 1a) The prominent signals in the spectrum originated from products of proinsulin Two insulin variants are expressed in mouse, insulin ... spectrometry of insulin J Am Chem Soc 104, 2948 36 Furuta, M., Carroll, R., Martin, S., Swift, H.H., Ravazzola, M., Orci, L & Steiner, D.F (1997) Incomplete processing of proinsulin to insulin accompanied ... affects the intracellular level of calcium and specific binding of C-peptide to plasma membrane has been observed During proinsulin conversion, insulin and C-peptides are produced in equimolar...
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Báo cáo Y học: Functional analysis of the rat bile salt export pump gene promoter Regulation by bile acids, drugs and endogenous compounds potx
Ngày tải lên :
17/03/2014, 23:20
... putative binding site for HNF3-b overlapping with the TATA box of transcription initiation could be found within the minimal Bsep promoter Additional consensus motifs of the minimal promoter include ... sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 13 Goodwin, B., Jones, S.A., Price, R.R., Watson, ... designated the minimal Bsep promoter, apparently containing all binding sites required for basal transcription The minimal promoter and larger constructs were only functional in the human hepatoblastoma...
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