fl 6th c queen of the franks

Tài liệu Báo cáo khoa học: Salt-induced formation of the A-state of ferricytochrome c – effect of the anion charge on protein structure docx

... eukaryotic c class cytochromes [27]; anions exert a strong inﬂuence on the lysine residues of cytochrome c, and signiﬁcantly affect the structure and the functional properties of the protein, as the conserved ... modulates the structure of the A state of cytochrome c Biochemistry 39, 12632– 12638 18 Sinibaldi F, Howes BD, Smulevich G, Ciaccio C, Coletta M & Santucci R (2003) Anion concentration modulates conformation ... Fig Fig Absorbance at 695 nm of acid-denatured cytochrome c in the presence of increasing sulfate (s) and selenate (d) concentrations The optical absorbance of native cytochrome c (—) at pH 7.0...

Ngày tải lên: 19/02/2014, 05:20

11 487 0

Tài liệu Báo cáo Y học: The glucose-speciﬁc carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx

... and (c) inactivation of EIIGlc is accelerated in the presence of Glc (see below) Although the dominant reactivity of Cys421 compromised the labelling of other active-site residues, the glucose ... II-BGlc, a glucose receptor of the bacterial phosphotransferase system: molecular cloning of ptsG and puriﬁcation of the receptor from an overproducing strain of Escherichia coli Proc Natl Acad Sci ... biphasic to a monophasic shape are Table Rates of inactivation of IICBGlc Incubation of puriﬁed IICBGlc with the indicated concentration (mM) of the analogues 1a)3d was carried out at 30 C Rate constants...

Ngày tải lên: 21/02/2014, 01:21

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Tài liệu Báo cáo Y học: CK2btes gene encodes a testis-speciﬁc isoform of the regulatory subunit of casein kinase 2 in Drosophila melanogaster potx

... ampliﬁed from the cDNA #911 using the following pair of primers: 5¢-CTGGATCCCT ATGTCGTGTCCCAGGAGCATCGAG-3¢ and 5¢-GTC TGCAGTTAAAAATTCGGGACATTCCTTAGCCA GG-3¢ BamHI–PstI digested PCR product was cloned ... following pair of primers: 5¢-CAGAATTCA TGACACTTCCTAGTGCGGCTCGC-3¢ and 5¢-CTG GATCCTTATTGCTGATTATTGGGATTCATTTGA CCA-3¢ EcoRI–BamHI digested PCR product was cloned as a fusion with the GAL4 activation ... using the following pair of primers: 5¢-GACTGCAGTGAAGG GCATCGAGTCCTCGGG-3¢ and 5¢-GAGGATCCGG GACATTCCTTAGCCAGGAGGG-3¢ To make the b-galactosidase expressing construct, a 173-bp PCR fragment of the...

Ngày tải lên: 21/02/2014, 15:20

10 465 0

Báo cáo khoa học: Speciﬁc cleavage of the DNase-I binding loop dramatically decreases the thermal stability of actin pot

... the open cleft conformation The cleavage within the D-loop enhances the nucleotide exchange [17] and increases accessibility of the cleft to limited proteolysis [5], which characterizes the cleft ... stabilizing the ﬁlament by closing the cleft in actin subunits [49,50] Hence, the increase in the thermal stability of ECP-cleaved F-actin and the disappearance of the shoulder on the DSC proﬁle can ... exceed ±10% G-actin Results Effects of the D-loop cleavage on the thermal unfolding of G-actin The excess heat capacity curves obtained for intact, ECP-cleaved, and subtilisin-cleaved ATP-Ca-G-actins...

Ngày tải lên: 06/03/2014, 22:21

11 483 0

Báo cáo " Non-commutative chern characters of the $C^*-$algebras of the sphers " ppt

Ngày tải lên: 14/03/2014, 13:20

11 243 0

Báo cáo khoa học: PKA independent and cell type speciﬁc activation of the expression of caudal homeobox gene Cdx-2 by cyclic AM pptx

... TGCACCACCAACTGCTTAG GCCATTCACAGGGCACATTC CCTCCTGATGGTGATGTATCGA TAACCACCGTAGTCCGGGTACT AGCGACTGTAGTGAAACTCCTTCTC AGCATCACCCATTTGATGT TCCACGACATACTCAGCAC GACGCAGGGATGATGTTC CCGGTTCCTCTTGGTGTTCA 2756 FEBS Journal ... Human CDX2 Rat GAPDH Mouse GAPDH Human GAPDH Rat proglucagon AAACCAGGACGAAAGACAAATACC GGACGTGAGCATGTATCCTAGCT CCTCGGCAGCCAAGTGAA TGATTCTACCCACGGCAAGT AACGACCCCTTCATTGAC TGCACCACCAACTGCTTAG GCCATTCACAGGGCACATTC ... HT-29 cell line by real time RT-PCR The nonendocrine colon cancer cell line HT-29 (A), or the endocrine cell line GLUTag (B), or the primary cell culture FRIC (C, D) were treated with either the control...

Ngày tải lên: 30/03/2014, 16:20

14 506 0

Báo cáo Y học: The cytoplasmic C-terminus of the sulfonylurea receptor is important for KATP channel function but is not key for complex assembly or trafﬁcking pdf

... the efficacy of drug response To further characterize this, dose–response curves were constructed for two of these and it was found that the Ki was not significantly affected once the differences ... on the images represents lm Ó FEBS 2002 Role of the cytoplasmic C- terminus of SUR1 (Eur J Biochem 269) 5311 Fig Analysis of the effect of coexpression of SUR1del249myc on Kir6.2 trafficking The ... trafficking assay It can be observed that coexpressing of channel subunits significantly changes the percentage of membrane associated fluorescence corresponding to both Kir6.2 and SUR1 The scale...

Ngày tải lên: 31/03/2014, 08:20

11 467 0

Báo cáo Y học: Structural study on lipid A and the O-speciﬁc polysaccharide of the lipopolysaccharide from a clinical isolate of Bacteroides vulgatus from a patient with Crohn’s disease ppt

... an acyoxyacyl substitution at N2-GlcNII Cleavage F indicated the chain length of the fatty acid on the acyoxyacyl group to be mainly 15, conﬁrming the result of the compositional analysis In the ... the structure of the lipid A moiety is similar to that of B fragilis but the polysaccharide part is completely different, the difference in structure of the polysaccharide region may reﬂect the ... studied the structure of the core saccharide, it would be made up of Gal and Glc The results of this study showed that the polysaccharide region of LPS from Bacteroides has wide structural variation...

Ngày tải lên: 31/03/2014, 23:20

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NHỊP ĐẬP CỦA TUYỆT ĐỐI (Bài nói về Ishavasya Upanishad) - Nguyên Tác: HEARTBEAT OF THE ABSOLUTE (I OSHO) Phần 10 ppt

... logic - đến c a hiệu c t t c vào sáng sớm để c t t c Ông c t t c xong Tiền c t t c năm mươi xu, nhà logic đưa cho ông thợ c t t c đồng ru pi Không c tiền trả lại, ông thợ c t t c bảo khách hôm ... Phật, c ch th c phân tích, c ch th c biện luận, c ch th c tranh luận C ch th c chìm vào quan hệ với thầy, trở thành hoà hợp với thầy, đồng với thầy Trong hoà hợp đó, chân thành đó, người ta vào c t ... thấy vô dạng? C c hiền nhân Upanishad nói, “Chỉ c chỗ, bên ông, nơi c a sổ chút nào.” Gạt sang bên vi c dùng gi c quan chúng tạo khung C c gi c quan c a sổ Nếu nhìn đâu với giúp đỡ c a sổ này,...