... further suggesting the independence of hemin bioactivity on the HMOX pathway Thus, neither statins- nor hemindependent suppression of proliferation involved the HMOX system; further, induction of ... inhibitory activity in all tested pancreatic cancer cell lines with cerivastatin being the most efficient of these agents One of the mechanisms possibly involved in anti-carcinogenic effect of ... most efficient statin in our study, was capable of inducing several ‘events’ involved in carcinogenesis, including apoptosis, ROS production and inhibition of K-Ras trafficking Hemin treatment not
Ngày tải lên: 21/09/2020, 01:22
... further suggesting the independence of hemin bioactivity on the HMOX pathway Thus, neither statins- nor hemindependent suppression of proliferation involved the HMOX system; further, induction of ... inhibitory activity in all tested pancreatic cancer cell lines with cerivastatin being the most efficient of these agents One of the mechanisms possibly involved in anti-carcinogenic effect of ... most efficient statin in our study, was capable of inducing several ‘events’ involved in carcinogenesis, including apoptosis, ROS production and inhibition of K-Ras trafficking Hemin treatment not
Ngày tải lên: 04/12/2022, 10:35
DSpace at VNU: Antiproliferative and Pro-Apoptotic Activity of Diarylheptanoids Isolated from the Bark of Alnus japonica in Human Leukemia Cell Lines
... caspase8 and -9 To further determine the involvement of caspases in Hir-induced apoptosis, the (A) (B) Figure Involvement of the caspase cascade in Hir-induced apoptosis (A) The effect of Hir on PARP ... therefore, we further investigated the detailed mechanisms underlying the antiproliferative activity of Hir in the Jurkat cell line 762 T UTO et al Table Anti-Proliferative Effects (IC50 ) of ... various periods, the harvested cells were lysed, and the supernatants were boiled for Protein concentration was determined using a dyebinding protein assay kit according to the manufacturer’s
Ngày tải lên: 16/12/2017, 10:05
Investigation of the key chemical structures involved in the anticancer activity of disulfiram in A549 non-small cell lung cancer cell line
... for the cytotoxicity of DS The above experiments confirmed the indispensible role of the thiol groups in the cytotoxicity of DS Furthermore, we modified the DDC chemical structure and examine the ... which are the active forms of DS with antialcoholism activity [26] In this study, we examined the in vitro cytotoxicity of S-Me-DDC, the major metabolites of DS in the liver In combination with ... medicine, demonstrated strong anticancer activity in the laboratory but did not show promising results in clinical trials The anticancer activity of DS is copper dependent The reaction of DS
Ngày tải lên: 03/07/2020, 01:05
Báo cáo khoa học: RNA-binding properties of HCF152, an Arabidopsis PPR protein involved in the processing of chloroplast RNA pdf
... located at the C-terminus of the protein although binding of the C-terminus half (CH) is only half of the full-length protein (Fig. 3). Interestingly, the binding efficiency of the full-length mutant ... and the possible protein–protein interactions of HCF152, we produced the protein and several truncated versions in bacteria, and analyzed its protein–protein interactions and RNA-binding properties. ... model of how HCF152 is involved in the stabilization of the splicing products of the petB intron could be that the protein binds to the UTR region between psbH and petB, and to domain IV of the
Ngày tải lên: 17/03/2014, 10:20
Báo cáo khoa học: Cell death-inducing DFF45-like effector, a lipid droplet-associated protein, might be involved in the differentiation of human adipocytes pdf
... analyses of the relative levels of the indicated proteins after western blotting (as in D) were carried out Similar experiments were performed five times and the intensity of the individual bands in ... However, the detailed roles of human CIDEC in the differentiation of human adipocytes remain unknown In the present study, we found that the expression of CIDEC increased during the differentiation of ... subcellular compartments other than lipid droplets The expression of CIDEC is elevated during the differentiation of adipocytes To gain further insight into the roles of CIDEC in adipocyte differentiation,
Ngày tải lên: 23/03/2014, 03:20
Báo cáo khoa học: Identification of domains involved in the allosteric regulation of cytosolic Arabidopsis thaliana NADP-malic enzymes ppt
... 2] or 20 l M of CoA or acetyl-CoA. The results are presented as the percentage of activity in the presence of the effectors in relation to the activity mea- sured in the absence of the metabolites ... than the other compounds (Fig. 6). In this way, amino acid res- idues of different segments of NADP-ME2 swapped in the construction of the chimeras are involved in the fumarate activation of the ... possible that in this chimera a change in the pKa value of the amino acid residue(s) involved in malate inhibition may occur as a result of interaction with different amino acid residues in the allosteric
Ngày tải lên: 23/03/2014, 04:20
Báo cáo khoa học: Protein kinase Ch activity is involved in the 2,3,7,8tetrachlorodibenzo-p-dioxin-induced signal transduction pathway leading to apoptosis in L-MAT, a human lymphoblastic T-cell line potx
... amount of PKCh (in 20 lg of total protein) expressed in L-MAT cells, which seemed to be around ngỈlg)1 of total L-MAT cell protein The lower panel shows the amount of protein PKCd (in 40 lg of total ... doses suggestive of inhibition of PKCd and PKCh These results indicate that nPKC (PKCd and ⁄ or PKCh) is involved in the TCDD-induced apoptosis of L-MAT cells The incubation of L-MAT cells with ... of protein) were sufficient to allow the detection of change of PKCd in the particulate fraction Finally, we sought to verify the functional involvement of PKCh kinase activity in TCDD-induced
Ngày tải lên: 23/03/2014, 13:20
Báo cáo khoa học: A new pathway encompassing calpain 3 and its newly identified substrate cardiac ankyrin repeat protein is involved in the regulation of the nuclear factor-jB pathway in skeletal muscle pdf
... involved in the transmission of mechanical signals to signaling path- ways. In the M line, mechanical stimulation activates the interaction of a protein complex with the kinase domain of titin, ... binding to titin by improving the accessibility of the titin-binding domain. Interestingly, the importance of CARP inter- action for its function was recently demonstrated in a Fig. 3. Calpain ... cell fate-governing NF-jB pathway. Interestingly, the expression of the atro- phying protein MURF1, one of NF-jB main targets, remains unchanged in presence of CARP, suggesting that the pathway encompassing
Ngày tải lên: 29/03/2014, 21:20
Báo cáo khoa học: Catalytic activation of human glucokinase by substrate binding – residue contacts involved in the binding of D-glucose to the super-open form and conformational transitions ppt
... residues were individually mutated (C) The spacial proximity of active site residues in the L-domain and connecting region II which are potentially involved in the binding of D-glucose in the super-open ... E290) and the S-domain (residues T168 and K169) (Fig 1B) To identify the contact residues involved in the initial binding of Glc to the super-open state (Fig 1C) of this two domain hinge-bending enzyme, ... considering the experimental error in determining the absorption coefficients at 280 nm for the two proteins No effect of Glc was observed on the fluorescence spectrum of the isolated GST protein (data
Ngày tải lên: 30/03/2014, 04:20
Báo cáo khoa học: Towards understanding the functional role of the glycosyltransferases involved in the biosynthesis of Moraxella catarrhalis lipooligosaccharide ppt
... irradiating each of the anomeric signals in turn to obtain the H chemicals shifts of the corresponding remaining ring protons Again, assignment of the Kdo residue was possible from examination of ... Lgt4 Results In order to investigate the function of the glycosyltransferase enzymes involved in the biosynthesis of LOS of M catarrhalis, and to gain some insights into the mechanism of serotype ... examination of the COSY spectra, as many of the ring protons for this residue lie outside the crowded 3.2–4.1 p.p.m region of the spectra For the other hexose residues, the anomeric and H2 protons
Ngày tải lên: 30/03/2014, 08:20
Báo cáo khoa học: Geranylgeranyl reductase involved in the biosynthesis of archaeal membrane lipids in the hyperthermophilic archaeon Archaeoglobus fulgidus potx
... the protein arose from the translation from Met at the 24th position of the expec- ted full-length of the archaeal enzyme (except for the polyhistidine-tag). In contrast, a distinct protein of slightly ... DGGGP. On the other hand, the expres- sion of the enzyme in E. coli was reported to have no effect on the quinone pro? ??le of the host, clearly indica- ting that menaquinone and ubiquinone are ... for the enzyme [21]. These facts strongly suggest the involvement of the enzyme, GGR, in the biosynthesis of membrane lipids in the hyper- thermophilic archaeon A. fulgidus, as predicted in the previous
Ngày tải lên: 30/03/2014, 09:20
Báo cáo khoa học: The GxxxG motif of the transmembrane domain of subunit e is involved in the dimerization/oligomerization of the yeast ATP synthase complex in the mitochondrial membrane doc
... eHis 6 ) were incubated in the presence or absence of CuCl 2 as described in the experimental pro- cedure. The control experiment (in the absence of CuCl 2 ) was performed in the presence of NEM instead of ... eG19L strains were incubated in the presence or absence of CuCl 2 . The control experiment (in the absence of CuCl 2 )was performed in the presence of NEM instead of CuCl 2 . Cross-linking conditions ... the stability of an oligomeric structure of the ATP synthase in digitonin extracts. The data show the involvement of the dimerization motif of subunit e in the formation of supra- molecular structures of
Ngày tải lên: 31/03/2014, 01:20
Báo cáo khoa học: Factors involved in the assembly of a functional molybdopyranopterin center in recombinant Comamonas acidovorans xanthine dehydrogenase pot
... means for their incorporation into the expressed protein in order to form an active enzyme Proteins functioning in the sulfuration of the molybdopyranopterin cofactor have been identified in D melanogaster ... expressed in R capsulatus The protein product of xdhC appears to exhibit a redox role in maturation of the XDHABC by eliminating the dependence of its functional expression on the redox state of the ... effect of the level of culture aeration on the expression of recombinant protein Raising the cellular redox potential by increasing aeration levels is expected to reduce the level of cysteine and increase...
Ngày tải lên: 16/03/2014, 23:20
Báo cáo y học: "A novel scheme to assess factors involved in the reproducibility of DNA-microarray data" pdf
... are pooled They are, thus, not accommodated in the VG interaction, but rather in the residual error The remaining 14.9 % variance in the VSG interaction still contains the Gene × Array interactions ... (S=1v23) in the VSG interaction decreased with 16.1 % to 14.9 %, with the variance in the VG interaction remaining virtually unchanged By combining S2 and S3, the Gene × Array interactions occurring ... show that the latter factors are indeed important for optimizing DNA-microarray data quality In order to assess the reproducibility of- and factors involved in DNA-microarray data produced in our...
Ngày tải lên: 14/08/2014, 14:21
Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 2
... the cloning of their shRNAs and measurements of OCT4 / Nanog promoter activity in their knocked down cells (See Appendix) The rationale behind the use of the luciferase assay is as such: In wild ... represent the processed form, ie the 34kDa form of total Lefty proteins, ie both Lefty1 and Lefty2 The intensities of the bands were compared to the loading control -Actin 51 2.1.2.3 RNAi knockdown of ... containing either the Oct4 promoter or Nanog promoter that contain OCT4/SOX2 binding sites driving the transcription of a luciferase reporter gene were used to measure the transcription activity of these...
Ngày tải lên: 11/09/2015, 16:02
Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 4
... the C-terminus of Lefty2 was chosen instead of the N-terminus because tagging at N-terminus may result in the V5 tag being cleaved off during LEFTY2 processing and secretion The insert was amplified ... for the entire duration of the experiment The E14 cells were fed daily with medium containing fresh Activin A 4.14.2 Inhibition of Nodal/Activin signaling To abolish/diminish Nodal/Activin signaling, ... overexpression The expression of the Nodal transgene was suppressed in the presence of 1µg/ml doxycyclin (Clontech,UK.) Hence to activate Nodal induction, the drug was removed from the cells by changing the...
Ngày tải lên: 11/09/2015, 16:02
Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 5
... (2007) Activin-Nodal signaling is involved in propagation of mouse embryonic stem cells J Cell Sci 120, 55-65 Oh, S P., and Li, E (1997) The signaling pathway mediated by the type IIB activin receptor ... Transcriptome profiling of human and murine ESCs identifies divergent paths required to maintain the stem cell state Stem Cells 23, 166-185 Wieduwilt Matthew J., (2003) SMAD3 in embryonic patterning, ... (2003) The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells Cell 113, 631642 Moffat, J., and Sabatini, D M (2006) Building mammalian signalling pathways...
Ngày tải lên: 11/09/2015, 16:02
Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency
... cysteine residues at their C-termini All of these residues are used for the formation of intrapeptide bonds with the exception of the third cysteine residue from the C terminus of mature proteins ... signaling Interestingly, they found that the OCT3/4 compartment is maintained in the controls, while the outgrowths cultured in the presence of SB431542 display loss of OCT3/4 staining They also ... types of the three germ layers in culture Another defining feature of ES cells is their acquired capacity to proliferate indefinitely in vitro without undergoing senescence while retaining a...
Ngày tải lên: 11/09/2015, 16:02
Appendix identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency
... • Rex2 • Zinc finger protein • • • Zfx • • • a-catenin • Zinc finger protein Transcription factor Link to canonical Wnt pathway Subunit of Cadherin protein Perturbed Nanog promoter activity when ... during retinoic acid induced differentiation of F9 cells High expression in ES cells Link to canonical Wnt pathway • • • • • • Secreted protein Member of TGFsuperfamily Involved in Nodal signaling ... presented in this thesis, the relative transcript levels for the Ct method gene of interest between test sample and control were tabulated using the -Actin was used as the housekeeping gene for...
Ngày tải lên: 11/09/2015, 16:03