factors involved in the pro oxidant activity of carotenoids
Báo cáo khoa học: Factors involved in the assembly of a functional molybdopyranopterin center in recombinant Comamonas acidovorans xanthine dehydrogenase pot
Ngày tải lên :
16/03/2014, 23:20
... means for their incorporation into the expressed protein in order to form an active enzyme Proteins functioning in the sulfuration of the molybdopyranopterin cofactor have been identified in D melanogaster ... expressed in R capsulatus The protein product of xdhC appears to exhibit a redox role in maturation of the XDHABC by eliminating the dependence of its functional expression on the redox state of the ... effect of the level of culture aeration on the expression of recombinant protein Raising the cellular redox potential by increasing aeration levels is expected to reduce the level of cysteine and increase...
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Báo cáo y học: "A novel scheme to assess factors involved in the reproducibility of DNA-microarray data" pdf
Ngày tải lên :
14/08/2014, 14:21
... are pooled They are, thus, not accommodated in the VG interaction, but rather in the residual error The remaining 14.9 % variance in the VSG interaction still contains the Gene × Array interactions ... (S=1v23) in the VSG interaction decreased with 16.1 % to 14.9 %, with the variance in the VG interaction remaining virtually unchanged By combining S2 and S3, the Gene × Array interactions occurring ... show that the latter factors are indeed important for optimizing DNA-microarray data quality In order to assess the reproducibility of- and factors involved in DNA-microarray data produced in our...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 2
Ngày tải lên :
11/09/2015, 16:02
... the cloning of their shRNAs and measurements of OCT4 / Nanog promoter activity in their knocked down cells (See Appendix) The rationale behind the use of the luciferase assay is as such: In wild ... represent the processed form, ie the 34kDa form of total Lefty proteins, ie both Lefty1 and Lefty2 The intensities of the bands were compared to the loading control -Actin 51 2.1.2.3 RNAi knockdown of ... containing either the Oct4 promoter or Nanog promoter that contain OCT4/SOX2 binding sites driving the transcription of a luciferase reporter gene were used to measure the transcription activity of these...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 3
Ngày tải lên :
11/09/2015, 16:02
... Conflicting roles of Nodal signaling in both the maintenance of ES cell self renewal and induction of ES cell differentiation have both previously been reported A key objective of this thesis ... addition of Activin A ligand causes differentiation of ES cells into meso/mesendodermal lineages while repression of signaling with the specific chemical inhibitor SB431542 and Lefty protein supplementation ... Nodal signaling might, as in the in vivo scenario, likewise influence ES cell fate decision in a dose dependent manner in vitro is addressed Interestingly, it was found that Nodal signaling is able...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 4
Ngày tải lên :
11/09/2015, 16:02
... the C-terminus of Lefty2 was chosen instead of the N-terminus because tagging at N-terminus may result in the V5 tag being cleaved off during LEFTY2 processing and secretion The insert was amplified ... for the entire duration of the experiment The E14 cells were fed daily with medium containing fresh Activin A 4.14.2 Inhibition of Nodal/Activin signaling To abolish/diminish Nodal/Activin signaling, ... overexpression The expression of the Nodal transgene was suppressed in the presence of 1µg/ml doxycyclin (Clontech,UK.) Hence to activate Nodal induction, the drug was removed from the cells by changing the...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 5
Ngày tải lên :
11/09/2015, 16:02
... (2007) Activin-Nodal signaling is involved in propagation of mouse embryonic stem cells J Cell Sci 120, 55-65 Oh, S P., and Li, E (1997) The signaling pathway mediated by the type IIB activin receptor ... Transcriptome profiling of human and murine ESCs identifies divergent paths required to maintain the stem cell state Stem Cells 23, 166-185 Wieduwilt Matthew J., (2003) SMAD3 in embryonic patterning, ... (2003) The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells Cell 113, 631642 Moffat, J., and Sabatini, D M (2006) Building mammalian signalling pathways...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency
Ngày tải lên :
11/09/2015, 16:02
... cysteine residues at their C-termini All of these residues are used for the formation of intrapeptide bonds with the exception of the third cysteine residue from the C terminus of mature proteins ... signaling Interestingly, they found that the OCT3/4 compartment is maintained in the controls, while the outgrowths cultured in the presence of SB431542 display loss of OCT3/4 staining They also ... types of the three germ layers in culture Another defining feature of ES cells is their acquired capacity to proliferate indefinitely in vitro without undergoing senescence while retaining a...
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Appendix identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency
Ngày tải lên :
11/09/2015, 16:03
... • Rex2 • Zinc finger protein • • • Zfx • • • a-catenin • Zinc finger protein Transcription factor Link to canonical Wnt pathway Subunit of Cadherin protein Perturbed Nanog promoter activity when ... during retinoic acid induced differentiation of F9 cells High expression in ES cells Link to canonical Wnt pathway • • • • • • Secreted protein Member of TGFsuperfamily Involved in Nodal signaling ... presented in this thesis, the relative transcript levels for the Ct method gene of interest between test sample and control were tabulated using the -Actin was used as the housekeeping gene for...
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Báo cáo y học: " Inhibition of SOC/Ca2+/NFAT pathway is involved in the anti-proliferative effect of sildenafil on pulmonary artery smooth muscle cells" pps
Ngày tải lên :
12/08/2014, 14:20
... (SOCE) in the membrane caused by ER depletion is the dominated component of CCE [16] Ca2+ influx via SOC appears to be a determinant in maintaining a sustained increase in [Ca2+]i and regulation of ... Nifedipine (1 μM, blocker of VDCC) had no effect on hypoxia-induced cell proliferation These data suggested that sustained entry of extracellular Ca2+ via SOC is the main pathway of maintaining the ... resulted in the enhancement of SOCE and elevation of [Ca2+]i The promoted [Ca2+]i leads to increased calcineurin phosphatase activity, which induces nuclear translocation of NFAT NFAT activation in...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
Ngày tải lên :
16/02/2014, 09:20
... implicated in other protein–protein interactions, a STAT protein DNA-binding domain and an SH2 domain, which binds phosphorylated tyrosine residues in the context of a longer peptide motif within a ... all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipper domain and fork-head domain of foxp3 In addition, for stat6 and foxp3, there were ... areas of amino acid conservation throughout the vertebrates Significant homology was seen in the putative T-box DNA-binding domain of t-bet, the STAT protein interaction domain, STAT protein all-alpha...
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Báo cáo khoa học: The starch-binding capacity of the noncatalytic SBD2 region and the interaction between the N- and C-terminal domains are involved in the modulation of the activity of starch synthase III fromArabidopsis thaliana pdf
Ngày tải lên :
22/03/2014, 21:20
... the synthesis of amylopectin, it has been suggested that SSI is mainly involved in the synthesis of small chains of this fraction Furthermore, SSIV has been found recently to be involved in the ... interaction between the N- and C-terminal domains of SSIII in vitro Mapping of the CD-binding region in the N-terminal SBDs In order to identify the SBD region required for the SBD–CD interaction, we ... mgÆmL)1), indicating that the addition of D123 increases the apparent affinity of the CD protein for the polysaccharide However, the CD+D123 protein only partially restored the Vmax value of the full-length...
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báo cáo khoa học: "The grapevine guard cell-related VvMYB60 transcription factor is involved in the regulation of stomatal activity and is differentially expressed in response to ABA and osmotic stress" ppt
Ngày tải lên :
11/08/2014, 11:21
... analysis of the mechanisms that regulate the expression of the VvMYB60 gene extends beyond the scope of this work, it will be intriguing to further investigate the expression profile of VvMYB60 in ... conditions, the lines expressing the VvMYB60 gene displayed a rate of water loss indistinguishable from the one observed in the wildtype, thus demonstrating complete rescue of the stomatal defects of the ... signature in the MYB domain, located in the loop connecting the R2 and R3 repeats, and the lack of the first (CtM1) of three C-terminal motifs present in all the other MYB proteins assigned to subgroup...
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Risks Involved in the Use of Herbal Products
Ngày tải lên :
25/10/2013, 05:20
... aspirin or other NSAIDs may increase the risk of bleeding Ginseng may intensify the effects of these drugs, causing an excessive decrease in blood sugar levels (hypoglycemia) Ginseng may intensify ... discrepancy exists in the antioxidant and other bioactivities of flavonoids, which are powerful in assays conducted in vitro; the 14 Risks Involved in the Use of Herbal Products 357 measured in vivo activities ... from their ability to inhibit phase I and induce phase II carcinogen metabolizing enzymes that initiate carcinogenesis They also inhibit the promotion stage of carcinogenesis by inhibiting oxygen...
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Tài liệu Báo cáo khoa học: Crystal structure of thiamindiphosphate-dependent indolepyruvate decarboxylase from Enterobacter cloacae, an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid doc
Ngày tải lên :
20/02/2014, 11:20
... structure Binding of the cofactors ThDP and Mg2+ The homo-tetrameric IPDC binds four molecules of the cofactors ThDP and Mg2+ The ThDP binding sites are located in narrow clefts at the interfaces ... responsible for binding and proper orientation of the aminopyrimidine ring, are conserved in all ThDP-dependent enzymes One of these, the hydrogen bond between the N1¢ atom of the pyrimidine ring of ThDP ... enzymes Substrate binding site and catalytic residues The active site cavity in IPDC extends from the thiazolium ring of the cofactor to the surface of the protein The entrance of the active site...
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Tài liệu Báo cáo Y học: Identification of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A201A from Streptomyces capreolus pdf
Ngày tải lên :
21/02/2014, 01:21
... additional genes encoding other proteins implicated in the biosynthesis of the aminonucleoside moiety of A201A Here we report the sequencing of a total of 6946 bp, which include the ata genes most ... pertain to it Further sequencing of the ata cluster could provide an insight into the biosynthetic pathway of the other moieties of A201A and provide probes which may be useful to identify genes of ... or proposed to be implicated in the biosynthesis of the aminonucleoside moiety of puromycin [6–8] Interestingly, their organization is identical to that of the pur cluster, with the exception of...
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Tài liệu Báo cáo Y học: Granule-bound starch synthase I A major enzyme involved in the biogenesis of B-crystallites in starch granules ppt
Ngày tải lên :
22/02/2014, 07:20
... listed in Table that most, if not all, of the amylose is synthesized in vivo when the rate of amylopectin synthesis becomes minimal At these moments the rates of amylopectin synthesis are only 5% of ... accumulate at the final stage of starch synthesis in the absence of concomitant amylopectin synthesis (reviewed in [41]) In vitro synthesis of amylose Because in Chlamydomonas amylose synthesis remains ... rate of amylopectin synthesis The increase in crystallinity witnessed during these first 12 h is in line with the high rates observed for amylopectin synthesis However between 12 and 58 h the rate...
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Báo cáo khoa học: Monomeric molten globule intermediate involved in the equilibrium unfolding of tetrameric duck d2-crystallin pdf
Ngày tải lên :
08/03/2014, 08:20
... is the dynamic quenching constant and fa was the fractional maximum accessible protein fluorescence ANS binding assay The exposed hydrophobic surfaces of the protein were assayed by incubating the ... d2-crystallin is equilibrated in GdnHCl solutions [15] The protein appears to dissociate into monomers before further unfolding occurs The detailed dissociation/unfolding mechanism of the protein remains ... monomers This unfolding process was investigated by quenching of intrinsic protein ANS is a sensitive probe commonly utilized to detect conformational changes of proteins especially in the molten globule...
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Báo cáo khoa học: Identification of two cysteine residues involved in the binding of UDP-GalNAc to UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) ppt
Ngày tải lên :
08/03/2014, 10:20
... [22,23], containing a cDNA encoding the insulin signal sequence and the Protein A-IgG binding domain, was inserted into NheI-HindIII digested pcDNA3.1, producing the vector, pInsProA pDN42 was ... GalNAc-T1, might be involved in the catalytic function of the enzyme To investigate whether the cysteine residues modified by PCMPS are involved in the binding of UDP-GalNAc, we treated recombinant GalNAc-T1 ... scanning and the intensity of each band was determined using the NIH Image software The enzymatic activity of the P-DN42 and mutant P-DN42 gene products was determined as described below The activity...
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Báo cáo khoa học: Oxidative stress is involved in the permeabilization of the inner membrane of brain mitochondria exposed to hypoxia/reoxygenation and low micromolar Ca2+ Lorenz Schild1 and Georg Reiser2 doc
Ngày tải lên :
16/03/2014, 22:20
... contribute to the induction of oxidative stress during ischemia ⁄ reperfusion in brain There is a growing body of information concerning the mechanism of ROS generation by the respiratory chain in mitochondria ... treatment The high degree of protection by ADP suggests that the mitochondrial injury is caused by opening of the mPTP There is evidence that ADP inhibits opening of the mPTP by occupying binding sites ... the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 48 Fabiato A & Fabiato F (1979) Calculator programs for computing the...
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Báo cáo khoa học: Amino acid residues on the surface of soybean 4-kDa peptide involved in the interaction with its binding protein potx
Ngày tải lên :
17/03/2014, 03:20
... that of the active area in insulin (Fig 7F) If the mechanism of the interaction between the 4-kDa peptide and 43-kDa protein has the minimum components of insulin–insulin receptor interaction, the ... to the insulin– insulin receptor interaction system Interaction of insulin with the 43-kDa protein Acknowledgements Similarly to the 4-kDa peptide, insulin is able to interact with the 43-kDa protein ... central role in binding to the 43-kDa protein and that the wall consisting of fragment and part of fragment contributes to binding of the 4-kDa peptide to the 43-kDa protein (Fig 7A,B,C) In Fig 6C,...
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