extraction isolation and purification from paw paw

... separated from each other by CCC Since K2 and K4 were close to K3 and K5, respectively, curcumol (2) and germacrone (3), and curzerene (4) and 1,8-cineole (5) were hardly separated from each ... or the difference between K2 and K1, and the reduction in band width Within the eight tested solvents systems, the differences between K2 and K3, and between K4 and K5 in solvent systems (petroleum ... H3-7), 1.21 (6H, s, H3-9 and H3-10 ), 1.36 (1H, m, H-4), 1.45 (2H, m, Ha-3 and Ha-5), 1.47 (2H, m, Ha-2 and Ha-6 ), 1.61 (2H, m, Hb-2 and Hb-6 ) and 2.00 (2H, m, Hb-3 and Hb-5 ) The 13C NMR spectrum...

Ngày tải lên: 07/03/2014, 21:20

... purified proteins; M, molecular weight standards For the molecular weight standards, the bands from top to bottom correspond to 116.0, 66.2, 45.0, 35.0, 25.0 and 18.4 kDa, respectively consistent ... wholecell extracts and the supernatant and precipitate fractions are shown in Fig It was found that E coli BL21(DE3) ⁄ pHYDJs and E coli BL21(DE3) ⁄ pHYDPf produced a predominant band with an apparent ... hydantoinases from Jannaschia sp CCS1, P fluorescens PfO-1 and S coelicolor A3(2) Cloning and expression of the putative hyd genes The deduced open reading frames of putative hyd genes were PCR-amplified from...

Ngày tải lên: 18/02/2014, 08:20

... mistletoe lectin I [viscumin] from Viscum album [7], modeccin [8] and volkensin [9] from the roots of Adenia digitata and Adenia volkensii, respectively, and pulchellin from Abrus pulchellus seeds ... isolated, and their amino acids sequences deduced by cDNA cloning and verified by MS Half maximal inhibitory concentration (IC50) and median lethal dose (LD50) values from HeLa cells and mice divided ... (types A+, B+ and O+), rabbit and horse where they showed blood group specificity and distinct hemagglutination activity P I and P II promoted hemagglutination of human erythrocytes at 22.5 and 27.5...

Ngày tải lên: 18/02/2014, 16:20

... curves Squares and dotted line: data and fitting with PHI/PHO ratio of 0.5 Triangles and continuous line: data and fitting with PHI/PHO ratio of Asterisks and broken line: data and fitting with ... xylene monooxygenase from Pseudomonas stutzeri [14] and alkene monooxygenase from Xantobacter Py2 [15] In other enzymes (MMO from M capsulatus and Methylocistis [5,7], T2M from Burkolderia cepacia ... components’ from two other PHs The N-terminal sequence of PHI from A radioresistens S13 is identical (from residue number 3) to the sequence of the corresponding component of PH from A calcoaceticus...

Ngày tải lên: 21/02/2014, 00:20

... identity and function The 3D structures of the penta-heme NrfA from E coli K-12 [16] and D desulfuricans ATCC 27774 are also available [17] In both cases, when compared with W succinogenes and S ... column, equilibrated and eluted in 0.1 M Tris/HCl, pH 7.6 and several common protein–protein dissociation salts (1 M sodium chloride, M urea and M guanidinium chloride) and ionic detergents (CHAPS, ... nm and 409 nm All reagents were from Merck, except Chaps, Zwittergent 3–10 and Zwittergent 3–16 that were purchased from Calbiochem The excess SDS was removed from samples using the CalbiosorbTM...

Ngày tải lên: 21/02/2014, 00:20

... proteins and also to the low efficiency of extraction Physical properties of lamjapin Lamjapin obtained from the Mono Q column migrated as a single band characterized by both 12% SDS/PAGE and isoelectric ... Vilter, H (1991) Extraction of proteins from material rich in anionic mucilages: partition and fractionation of vanadatedependent bromoperoxidases from the brown algae Laminaria digitata and L saccharina ... SRD RNA and the mutant SRD RNA (A20 to G20) were labeled with [3H]adenine and treated with lamjapin and aniline The result revealed that lamjapin could release the 3H-labeled adenine from the...

Ngày tải lên: 08/03/2014, 10:20

... f and m from spinach, because no Trx f from Chlamydomonas has been isolated until now Both were active with Ch-Prx1, but while Trx f was as ef®cient as Trx h and m from Chlamydomonas, Trx m from ... previously [21] Chloroplastic thioredoxins m and f from spinach were a kind gift of P Schurmann (University È of Neuchatel, Switzerland) Thioredoxins m and h from à Chlamydomonas were puri®ed as described ... NcoI and BamHI, and cloned into the pET-8c vector Screening of a cDNA library A kgt11 cDNA library of Chlamydomonas was a gift from  Á M Goldschmidt-Clermont (Universite de Geneve, Switzerland)...

Ngày tải lên: 08/03/2014, 16:20

... prepared from Arabidopsis suspension cultures [49,50] and chloroplasts were isolated from green Arabidopsis plants [51] The purity and the intactness of 1301 D-cysteine desulfhydrase from a higher ... higher d-CDes mRNA and protein levels in older plants, during the light phase in a diurnal light ⁄ dark rhythm and under sulfate limitation Results In silico characterization and isolation of the ... molecular mass of 41.7 kDa and a pI of 6.34 The YedO protein from E coli and the d-CDes from Arabidopsis showed an overall identity of 36% and a similarity of 50% The blastp program in its default...

Ngày tải lên: 16/03/2014, 18:20

... blue-native (BN) ⁄ PAGE and (2) colorlessnative (CN) ⁄ PAGE and BN ⁄ PAGE, we separated the Ndh complex from both MS and BS chloroplasts and determined its monomeric and dimeric state We also ... exists as a monomer and dimer and splits into a membrane and soluble subcomplexes Separation of the Ndh complex by CN / PAGE (1D) and Tricine / PAGE (2D) and CN / PAGE (1D) and BN / PAGE (2D) ... obtained by Ndh antibodies, the gel bands that resulted from BN ⁄ PAGE (1D) and Tricine ⁄ PAGE (2D) or from CN ⁄ PAGE (1D), BN ⁄ PAGE (2D) and Tricine ⁄ PAGE (3D), and which corresponded to Ndh subunits,...

Ngày tải lên: 23/03/2014, 13:20

... ribosomal protein L40 from Ictaluridae punctatus [18], the ribosomal protein L40 family domain from Oncorhynchus mykiss, Pagrus major and Sebastes schlegli, and an unnamed product from Tetraodon nigroviridis ... with lysozyme and flounder pleurocidin Antimicrob Agents Chemother 45, 1337–1342 Waga S, Tan EM & Rubin RL (1987) Identification and isolation of soluble histones from bovine milk and serum Biochem ... collected from the skin exhibited high antimicrobial activity against Gram-positive and Gram-negative bacteria, as well as against the yeast C albicans As seen in Fig and Table 1, the antiCandida...

Ngày tải lên: 23/03/2014, 15:21

... values of Km and kcat are: (0.4 ± 0.2) mM and (0.91 ± 0.02) s)1 for Fe(III)EGTA (1.6 ± 0.8) mM and (0.16 ± 0.03) s)1 for Fe(III)EDTA (0.4 ± 0.2) mM and (0.08 ± 0.01) s)1 for Fe(III)citrate, and (0.4 ... molecular mass markers were from Fluka Non-denaturing electrophoresis was carried out in the same manner, but with omission of SDS from the gel running and loading buffers, and the sample was not ... mM ferrozine and 0.4 mM Fe(III) complexed with an appropriate ligand After : mixing, changes in absorbance at 562 nm were recorded Data from at least six repetitions were averaged and processed...

Ngày tải lên: 30/03/2014, 13:20

... extractions were attempted at 60 bar and 20ºC These conditions were found to be as effective as supercritical extractions and extraction yields in excess of 96 % in 15 minutes were obtained Extractions ... Picture of A) small LCD display and B) Processing methods for extraction Although the screens appeared to be identical and from the same batch, they were in fact different and on analysis it became ... supercritical conditions of 250 bar and 40ºC the extraction recovery from complete screens was poor, 0.1% of the total liquid crystal present The removal of a resin seal from the outer edge of the LCD...

Ngày tải lên: 28/04/2014, 10:50

... long-standing primary human FB cultures from endoscopic biopsies In the absence of standardised systems, we based FBs extraction method on those used for cultures from surgical pieces, muscle and ... for cytokeratin 20 in all the cultured and examined cells (Figure 3) Discussion Q-banding analysis of FBs from CD and non-CD subjects demonstrated a normal and stable karyotype (data not shown) ... obtained from CD patients and those from control subjects All the cultured cells were FBs with normal karyotype, as demonstrated by the FSP positivity, cytokeratine 20 negativity, and the Q banding...

Ngày tải lên: 18/06/2014, 15:20

... 1,2-monooxygenase from P putida NCIMB 10007 in Escherichia coli followed by simplified purification via affinity chromatography and characterization of the enzyme Material and methods Enzymes, chemicals and ... media Pfu+-polymerase was obtained from Roboclon (Berlin, Germany) and dNTPs from Roth (Karlsruhe, Germany) Restriction enzymes were obtained from New England Biolabs (Beverly, MA, USA) For SDS-PAGE ... DNA -purification from PCR, the MinElute PCR -purification Kit by Qiagen (Hilden, Germany) was used Furthermore the Miniprep Kit from Qiagen was used for plasmid purification HisTrap mL FF columns and Sephadex...

Ngày tải lên: 21/06/2014, 02:20

... throughout the extraction period The extraction was performed at 90 W (extraction solvent: ethanol) and 60 W (extraction solvent: acetone) microwave power After the pre-defined extraction period ... curcuma powder, ( ) % extraction of curcumin using ethanol from water soaked curcuma powder and ( ) % extraction of curcumin using acetone from water soaked curcuma powder The extraction rate constant ... Separation and Purification Technology 79 (2011) 50–55 Fig Chromatographic profiles of curcumin: curcumin standard (A) and curcumin obtained after Soxhlet assisted extraction (B), microwave assisted extraction...

Ngày tải lên: 01/07/2014, 13:23

... Mm 04 ,lCH-sirT Mm 01 ,PTNd Mµ 052 ,esaremylop AND qaT U 1( ximerP RCP rewoPuccA dna )ASU ,reenoiB( remirp hcae noitartnecnoc Mµ 50.0 a ,etalpmet AND gniniatnoc serutxim noitcaer lµ 02 ni demrofrep ... eht fo esahp suoeuqa reppu eht no detcelloc saw AND lairetcaB nim rof 000,6 ta degufirtnec dna nim rof sllec lairetcab gniliob yb detcartxe neht saw AND lairetcab ;retaw dellitsid elbuod dezilirets ... ,A radlE ,A niktolZ 92 82-32 ,21 ,1991 loiborciM lppA tteL ANRr gnidocne AND fo msihpromylop no desab fo gnitnirpregnif AND DM snilloC ,MA smailliW 82 094-584 eaievrag succocotcaL sirebu succocotpertS...

Ngày tải lên: 07/08/2014, 18:21

... fragment was amplified from tissues and infected FCWF cells, while PCR for non-infected FCWF cells yielded no results By sequencing and analysis, the sequences from tissues and infected FCWF cells ... CCV from captive pandas by neutralization assay However to date, there have been no published reports of CCV infection in pandas In this study, we isolated a strain of CCV from two giant pandas, ... neutralize 100 TCID50 of the virus Total RNA was extracted from the giant pandas’ spleens, infected and non-infected FCWF cells (as positive and negative controls) using the TRizol Reagents kit (Invitrogen,...

Ngày tải lên: 07/08/2014, 23:22

... gel electrophoresis and specific bands were excised and purified using EzWayTM gel extraction kit (Komabiotech, Korea) Each purified amplicon was sequenced in both forward and reverse directions ... NA and HA genes, where they were sequenced directly after gel purification Sequences were deposited in GenBank under accession numbers; GU371911 and GU371912 for HA and NA respectively The HA and ... we report the isolation of HPAI H5N1 from donkeys living in contact with diseased birds and demonstrate the presence of H5 seropositive ones in the neighbouring areas Methods Virus isolation Nasal...

Ngày tải lên: 10/08/2014, 05:21

... mays (maize), and Saccharum sp (sugar cane) from the Poaceae family, R sativus and Brassica napus L (rapeseed) from the Brassicaceae family, and Basella alba 'Rubra' (B rubra) from the Basellaceae ... were disinfected and placed in a pot with soil and cultured Plants were grown at 20°C and 40 μE/ m2/s (12 h light; 12 h dark) for R sativus, T asetivum and B napus, and at 30°C and 133 μE/m2/s ... and CH3SCN were 1.48 and 4.48 min, respectively, and the products were quantified using the peak area To calibrate the concentrations of the products, gas and liquid standards of CH3I, DMS, and...

Ngày tải lên: 12/08/2014, 03:21

... (THA30F and THA382R), lipoxygenase Pplox1 (LOX982F and LOX1267R) and the actin Ppact7 (ACT-F and ACT-R) genes are shown in Table Primers used to amplify a 323 bp fragment of the cDNA from the ... growth and fruit quality Authors' contributions AT: identified and cloned the promoters AT, MS, LM and HS drafted the manuscript AT and MS: performed the digital expression analysis AM and AT: ... products were cloned in pGEM-T vector and sequenced (Macrogen, Korea) The Ppbec1 and Ppxero2 promoters were subsequently amplified from the pGEMT clones using the AP2 and BEC-32BamHI or DX24BamHI primers,...

Ngày tải lên: 12/08/2014, 03:21