isolation and purification of rabbit osteoclasts

... control of SMC proliferation 4466 S Bousserouel et al (Eur J Biochem 271) Modulation of cyclin D1 synthesis and hyperphosphorylation of Rb by n-3 and n-6 PUFAs Induction of cyclin D1 is one of the ... (Gastrointestinal Unit, University of Pennsylvania, Philadelphia, PA, USA) Isolation and culture of rat aorta SMC Vascular SMC were isolated by enzymatic digestion of the media of thoracic aortas removed ... Rb and Egr-1, in order to determine how the incorporation of EPA and DHA modulate SMC proliferation This article describes the differing effects of n-3 and n-6 PUFAs on the proliferation of SMC...

Ngày tải lên: 19/02/2014, 16:20

... Rz-1, Rz-2 and with their inactive versions (Rz-1i and Rz-2i) Transfections of the cells with lipofectin (NO) only or lipofectin and an empty plasmid (EM) were used as controls The level of the RT/PCR ... tRNAVal-driven and CTE helicaseassociated hammerhead ribozymes as efficient molecular tools for the inhibition of the biosynthesis of b-secretase and for the exclusion of the formation of toxic b-amyloid ... several months without any decomposition of the RNA Determination of the level of BACE mRNA and ribozyme RNA expression in mammalian cells The level of BACE mRNA and ribozyme RNA was monitored by RT/PCR...

Ngày tải lên: 08/03/2014, 08:20

... Each band was analysed densitometrically and the values are depicted after normalization of PEPCK and G6Pase bands with those of 18S rRNA (B) Each point is the mean ± SEM of three sets of experiments ... times Cells in the absence of any of these were taken as the control (Con) Two micrograms of total RNA were reverse transcribed with random primers and the levels of PEPCK and G6Pase mRNA were measured ... a series of events beginning with the TNFa-mediated prevention of the effect of insulin on Foxa2 localization and leading to the abrogation of insulin inhibition of gluconeogenesis and glucose...

Ngày tải lên: 16/03/2014, 02:20

... absence of O2, when the flavin cofactor is reduced [16–19] Thus, under anaerobic conditions in the absence of ammonium, reduction of the flavin moiety of NifL is required to relieve NifL inhibition of ... reduction of purified NifL using artificial electron donors or NADH and (b) analyzed the effect on nif-regulation of different membranebound oxidoreductases of the anaerobic respiratory chain and of terminal ... 280 and 320 nm (MD) Preparation of inside-out vesicles of K pneumoniae One litre cultures of K pneumoniae cells were grown under nitrogen- and O2-limited conditions, harvested at a D600 value of...

Ngày tải lên: 17/03/2014, 10:20

... donors and donors' families for their kind donation of bone tissue used for this study and offer an extended appreciation to the orthopaedic surgeons and nursing staff of the Department of Orthopaedics ... of Orthopaedics and Trauma in the Royal Adelaide Hospital for support and cooperation in the collection of femoral specimens and to the mortuary staff of the Institute of Medical and Veterinary ... differential gene expression of the α1 and α2 chain of collagen type I (p < 0.0001 and p < 0.002, respectively) between OA and controls, are shown in Table Interestingly, the ratio of COL1A1/COL1A2 was...

Ngày tải lên: 09/08/2014, 08:23

... tissue culture flasks for the inoculation of virus isolate at a multiplicity of infection (m.o.i.) of one After hour of adsorption, the virus was removed and ml of fresh RPMI-1640 media with 2% FBS, ... assays and IMTC participated in virus culture and virus isolation WYL was responsible for experimental design, analyses and drafting of the manuscript PKSC was responsible for design and supervision ... chemokine response profile varied with the time-course of infection Our results showed that at the early phase of avian influenza virus infection, the transcription of TNF-a and IL-6 was induced...

Ngày tải lên: 12/08/2014, 02:20

... also found Because TLR is the product of TLRi and RA, this can be the result of the negative correlation of RA and RS and the lack of correlation between TLRi and RS However, no correlation Genome ... blue) between RA and PA, PA and TLR or TLRi, RA and TR and between TR and TLR or TLRi Some of these correlations have been described previously [8,11,17,19] The correlation between TR and TLR was ... sizes were equal to that of genes in each considered group and subsequent computation of average and standard deviations for each variable An estimation of the average standard deviations (aSE)...

Ngày tải lên: 14/08/2014, 08:20

... probe RESULTS AND DISCUSSION A total of 69 well-banded chromosome spreads were scored Among 579 grains counted on the chromosomes (Fig 1), 500 (86%) were observed on chromosome and out of the 500 ... locate the silver grains and identify the chromosomes The chromosomes were classified according to the G-band pattern, defined by the Committee for the standardized karyotype of the Domestic Pig (1988) ... the presence of a 1,000-fold excess of sonicated salmon sperm DNA The precipitate was diluted in the following solution: 50% ionized formamide; 10% dextran sulfate; and 1/5 volume of SCCP (0.2...

Ngày tải lên: 14/08/2014, 19:23

... other (in case of lymphoma h1) and SMG-1, the gene of ribosomal protein S8 (in case of lymphoma h2), as well as several genes of unknown function (9 and in the case of lymphoma h1 and h2, respectively) ... analysis of the transcription levels of the set, ND4, and SMG-1 genes in human spontaneous lymphomas and normal B- and Tlymphocytes (a) 32P-labeled PCR-fragments of the set and a-myb oncogenes, and ... cDNA population of lymphoma h1 as tracer, and the cDNA population of lymphoma h2 as driver; and vice versa, 2) the cDNA population of lymphoma h2 as tracer, and the cDNA population of lymphoma h1...

Ngày tải lên: 02/11/2012, 11:08

... understanding of the influence of the molecular function is necessary, as well as of biochemical processes and of the biophysical properties provoked amongst others by a moderate repression of the ... block and the software version 1.2 The 10µl reaction consisted of 25ng of transcribed RNA (1µl), 1pmol Universal Probe (0.1µl), 50pmol each of forward + reverse primer (0.5µl), water (3.4µl) and ... the behaviour of the cells, which may alter the biochemical effect of the transported cargo and which could consequently influence the interpretation of the results Table List of the examined...

Ngày tải lên: 03/11/2012, 11:44

... representative TEM images of mitochondria in eWAT (Figures 2A and 2B) and iBAT (Figures 2C and 2D), respectively, and the analyses of mitochondria number (Figure 2E) and area (Figure 2F) In the ... The number and area of mitochondria in the eWAT and iBAT A-B Representative TEM images of eWAT C-D Representative TEM images of iBAT (Arrows point to mitochondria) E: The analysis of mitochondrial ... on our understanding of the adverse effects of particulate air pollution on cardiometabolic diseases, especially in the context of obesity and insulin resistance Xu et al Particle and Fibre Toxicology...

Ngày tải lên: 06/03/2014, 19:20

... date, four isoforms of PDK (PDK1–4) and two isoforms of PDP (PDP1–2) have been identified in humans [3–5] These isoforms display unique tissue distribution [3–5] and varied kinetic and regulatory ... Regulation of hPDK2 and hPDK4 gene expression Table The effects of PPAR a, d and c agonists on PDK2 and PDK4 transcript abundance expressed as a percentage of basal levels Results are the means ± SEM of ... 50 pmol of each gene-specific primer, ng of double-stranded cDNA, dNTPs (200 lm), buffers and 0.5 U of Expand High Fidelity Polymerase (Roche Diagnostics Ltd, Lewes, UK) in a final volume of 100...

Ngày tải lên: 07/03/2014, 17:20

... mixture of the two types of probes For analysis of differential gene expression, 1473 and 1703 beads were collected in fractions U1 and U2 of the up-regulated genes, and 3550 beads in fraction D of ... some of them might be missorted at the gates, which would expand the list of genes Of the beads collected at gates U1 and D on fluid microarray analysis, 89 and 87% were regarded as up-regulated and ... incubated in the presence (lanes and 4) or absence of TM (lanes and 2) for 48 h Proteins were extracted, digested with (lanes and 4) or without (lanes and 3) Endo H, and then subjected to SDS ⁄ PAGE...

Ngày tải lên: 07/03/2014, 21:20

... Protein and total RNA were extracted from various EOC cell lines, HEK293 and LNCaP cell lines (A, B) Expression levels of protein and mRNA, and levels of p53, ANX4 and the known p53 targets, p21 and ... Protein and RNA were extracted from two CCA (OVTOKO and OVISE) cells lines and one non-CCA (MCAS) cell line, and then ANX4 protein (A) and mRNA (B) levels were compared by western blotting and real-time ... HEK293 and LNCaP cell lines expressed trace amounts of ANX4 (Fig 5) Expression levels of p21 or MDM2 are higher in CCA p53 is a positive regulator of annexin IV cell lines than those of HEK293 and...

Ngày tải lên: 22/03/2014, 16:20

... differences in mRNA levels of four enzymes of the urea cycle Whereas the zonal-specific expression of the main enzymes of drug and xenobiotic metabolism has been the subject of extensive research (e.g ... cycle and were killed between and 11 a.m to avoid circadian influences Animals received humane care, and protocols complied with institutional guidelines Isolation of hepatocytes Periportal and ... cells, code for 6-phosphofructo-2-kinase, which produces fructose-2,6-bisphosphate, an allosteric activator of the glycolytic enzyme 6-phosphofructo-1kinase and an inhibitor of the gluconeogenic...

Ngày tải lên: 23/03/2014, 10:20

... B1 isoform) of other insect EcRs and a strong degree of identity was confined to the two ends of N-terminal region of the A/B domain Using a B1 isoform-specific probe from the A/B region of PIEcR, ... induction of mRNAs, the level of PIE75-B, PHR3 and PIEcR-B1 mRNAs was assessed after 1, and 18 h exposure, respectively Fig 8B and C show that after h and h exposure to 20E, the amount of accumulated ... efficiently the effects of 20E both on cell proliferation and morphological changes of IAL-PID2 cells Isolation and characterization of P interpunctella EcR-B1 mRNA Cloning of a PIEcR cDNA frament...

Ngày tải lên: 23/03/2014, 13:20

... total of 20 lL reaction volume containing lL of cDNA, lL of each of forward and reverse sequence specific primers (from 10 lm primer stock), 10 lL of supermix (Bio-Rad Laboratories) and lL of nuclease ... ecdysteroids and ecdysone mimics can induce and ⁄ or suppress endogenous genes in RKO and other mammalian cells and promote apoptosis A more comprehensive understanding of possible pleiotropic effects of ... effects of EcR switch inducers in mammalian cells We studied the effect of eight EcR ligands: four diacylhydrazines, two ecdysteroids, one THQ and one AMK ligand (Fig 1) on the expression of endogenous...

Ngày tải lên: 30/03/2014, 03:20

... analysis and drafted the manuscript KE, TB and KL carried out and supervised the ISH SWS and KE scored the cores KAS, SAS, SA and HS contributed in the clinical and demographic database and KAS and ... 2Institute of Clinical Medicine, University of Tromso, Tromso, Norway 3Department of Pathology, University Hospital of North Norway, Tromso, Norway 4Institute of Medical Biology, University of Tromso, ... (SCC) and negative lymph node status (N0); (B) SCC and positive lymph node status (N+); (C) adenocarcinomas (AC) and N0; (D) AC and N+ Author details Department of Oncology, University Hospital of...

Ngày tải lên: 18/06/2014, 16:20

... μl of the 2X QuantiTect™ SYBR® green master mix, 0.1 μl of HK-UNG (Epicentre, Madison, WI), and μl of RNase-free water for each sample for a total volume of 20 μl The PCR profile consisted of ... observed in the HP content of the tissues between ACL-X and control joints for any of the regions tested Error bars indicate standard error of the mean Values are μg of HP/mg of tissue wet weight Differential ... observed in the GAG content of the tissues between ACL-X and control joints for any of the regions tested Error bars indicate standard error of the mean Values are μg of GAG/mg of tissue wet weight...

Ngày tải lên: 20/06/2014, 00:20