expression systems regulated gene expression

... PPARa-target genes Promoter structure of the 17b-HSD-11 gene and transcriptional regulation All the above data strongly suggest that expression of the 17b-HSD-11 gene is directly regulated by ... Discussion SDS/PAGE analysis of the proteins whose expression levels were regulated by PPARa and its ligand To detect the protein bands whose expression levels were markedly altered by administration ... Motojima, K., Passilly, P., Peters, J.M., Gonzalez, F.J & Latruffe, N (1998) Expression of putative fatty acid transporter genes are regulated by peroxisome proliferator-activated receptor a and c activators...

Ngày tải lên: 16/03/2014, 18:20

... cases (1) Gene X’s expression and Gene Y’s expression are uncorrelated in both time and frequency domains (2) Gene X and Y expressions are uncorrelated in time domain, but gene X’s expression ... Validation of Clustering Scheme Given N genes with their expression or spectral density information EURASIP Journal on Bioinformatics and Systems Biology 1: Input gene expressions or spectral densities, ... learning At iteration t, a randomly selected gene s expression vector x is fed to the learning system, and the centroid which is closest to the coming gene s expression vector is represented in terms...

Ngày tải lên: 22/06/2014, 00:20

... downregulated genes and 68 upregulated genes were categorized into the functional category of immune response Most of the 68 upregulated genes were interferon regulated – including 17 IFI genes ... interferon -regulated genes This result was in agreement with a previous report showing that peripheral blood from SLE patients had remarkably homogeneous gene expression patterns with an overexpression ... IFI genes [10], and confirms the involvement of interferon in SLE Since the immune system is regulated by an elaborate network, interactions among the downregulated genes and the upregulated genes...

Ngày tải lên: 09/08/2014, 01:22

... downregulated genes and 68 upregulated genes were categorized into the functional category of immune response Most of the 68 upregulated genes were interferon regulated – including 17 IFI genes ... interferon -regulated genes This result was in agreement with a previous report showing that peripheral blood from SLE patients had remarkably homogeneous gene expression patterns with an overexpression ... IFI genes [10], and confirms the involvement of interferon in SLE Since the immune system is regulated by an elaborate network, interactions among the downregulated genes and the upregulated genes...

Ngày tải lên: 09/08/2014, 13:22

... between gene amplification and chromosomal deviations in malignant human gliomas Cancer Genet Cytogenet 1987, 29:165-170 Comprehensive genomic characterization defines human glioblastoma genes ... a higher expression of Cylin D1 was observed in high-grade tumor tissues than that of low-grade tumor tissues (Figure 5C) The expression of Cylin D1 reversely correlates with CDKN2A expression ... CDKN2A negatively regulated pRb and down -regulated level of cell cycle regulatory protein cyclin D1 Western blot analysis revealed a markedly lower phosphorylation of pRb and expression of cyclin...

Ngày tải lên: 10/08/2014, 10:21

... employed in our gene expression studies Amongst the differentially expressed genes, we focused on the study of p16INK4A (p16) and N-myc downstream regulated gene (NDRG1) as these two genes showed ... Literature Review 1.5 A newly identified up -regulated gene in cervical cancer: N-myc downstream regulated gene (NDRG1) 1.5.1 The human NDRG1 gene The human NDRG1 gene is also known as Drg-1, Cap43, RTP, ... cervical cancer 26 1.5 A newly identified up -regulated gene in cervical cancer: N-myc downstream regulated gene (NDRG1) 28 1.5.1 The human NDRG1 gene 28 1.5.2 Induction of NDRG1 by various agents...

Ngày tải lên: 14/09/2015, 11:19

... HLA-DRB1*04 genes and cytokine genes can modify the disease pattern in rheumatoid arthritis We also postulate that this may be due to genetic polymorphisms resulting in differential gene expression ... effect of IL-18 22 1.1.9 IL-18 promoter gene polymorphisms Recently, it was reported that IL-18 protein expression is regulated by the IL-18 promoter gene (Tone et al., 1997; Marshall et al., ... inflammatory disease of unknown etiology Genetic and environmental factors play an important role in its pathogenesis Genetically, the most thoroughly studied genes are those located on the major...

Ngày tải lên: 14/09/2015, 18:28

... EMBEDDED LINUX Just typing mount shows all the file systems mounted Most Linux systems have several file systems mounted, but all the file systems are mounted relative to the root file system ... Selecting a Root File System 313 Block-Based File Systems 314 MTD File Systems 318 RAM Buffer–Based File Systems 319 File System Pairings ... Pro Linux Embedded Systems ■■■ Gene Sally Pro Lin u x E m bedd ed S yst em s Copyright © 2010 by Gene Sally All rights reserved No part of this work may be...

Ngày tải lên: 08/03/2016, 09:24

... of the 3¢-untranslated region of mouse VEGF mRNA on gene expression in a heterologous reporter We evaluated the role of the 3¢ UTR in gene expression by introducing the SmaI ⁄ XbaI fragment (nt ... post-transcriptional stimulation of VEGF gene expression by agents such as LPS and VEGF not act through TNFa by an autocrine mechanism Activation of VEGF gene expression in RAW-264.7 cells with LPS ... Ziegler-Heitbrock L & Lewis CE (2003) Hypoxiainduced gene expression in human macrophages: implications for ischemic tissues and hypoxia -regulated gene therapy Am J Pathol 163, 1233–1243 10 Xiong...

Ngày tải lên: 07/03/2014, 12:20

... regulate the expression of genes playing key roles in the biogenesis of the OXPHOS system In general, the transcription of nuclear genes encoding proteins involved in OXPHOS biogenesis is controlled ... a-F1ATPase gene expression is particularly interesting given the significant number of genes regulated by SIN3 (a histone deacetylase complex) that are involved in mitochondrial energy-generating ... several genes in the recent years [39–41] 3¢ RNA processing of the D melanogaster a-F1-ATPase transcript The expression of the a-F1-ATPase gene during development is coordinated with the expression...

Ngày tải lên: 07/03/2014, 16:20

... the expression profiles, here, we focus on the gene- gene interactions characterized by the “apoptosis” and the “cell growth.” Among the gene- gene interactions listed in Figure 2, the gene- gene ... to gene expression profiles; here the expression profile indicates a set of the expression degrees of one gene, measured under various conditions This is because the profiles often share similar expression ... gene- gene interactions in the inferred network indicates the relationship between cancer and cell growth/death Thus, the gene systems network may also be useful as a bridge between the gene- gene...

Ngày tải lên: 22/06/2014, 19:20

... the rapid expansion of a systems- level understanding of gene expression These technologies are revealing the importance of noncoding RNAs and their role in regulating gene expression, as well as ... development stages) and generating profiles of fluorescence intensity along the worm body axis This high-throughput analysis generates spatiotemporal profiles of gene expression In a preliminary ... to the segmentation gene network in Drosophila, his approach recovered the correct expression patterns for 80% of cis-regulatory modules Spatio-temporal patterns of gene expression in multi-cellular...

Ngày tải lên: 14/08/2014, 07:21

... LHβ gene expression and the secretion of LH On the other hand, lowering the GnRH frequency results in the decline of LHβ gene expression and LH secretion but induces a rise in FSHβ gene expression ... βsubunit genes [37, 38] To generate the αT3-1 cell-line, immature gonadotrope cells were immortalized through directed oncogenesis in transgenic mice In particular, targeted expression of the oncogene ... gonadotropin-subunit gene expression 99 4.1.10 JNK-positive feedforward without ERK5-negative feedback on GnRHR expression causes loss of differential gonadotropin-subunit gene expression in the...

Ngày tải lên: 11/09/2015, 09:11

... Gene- specific primers for P falciparum ldh [9] and csp [10] genes were synthesised by Life Technologies and used in a reaction mixture using SYBR® Green PCR Core reagents kit (PE Applied Biosystems) ... showed no major differences among them and expression levels of the internal control were similar in the different experiments The expression levels of csp gene were depressed when parasites were ... histocompatibility complex class II expression by disruption of the Jak/Stat pathway J Exp Med 1998; 187: 675-683 21 Lidbury BA, Mahalingam S Specific ablation of antiviral gene expression in macrophages...

Ngày tải lên: 02/11/2012, 10:14

... oncogene, constant part of the λIg gene, the mitochondrial genes of NADH dehydrogenase subunit (ND4), the interferon-inducible gene 6-16 (Inf-ind), the 16S rRNA gene (most probably the humanin gene ... the calpain subunit gene, ND4 – the NADH dehydrogenase subunit gene, IL5 – the interleukin gene, λIg – the λ-chain of immunoglobulin gene, γIg – the γ-chain of immunoglobulin gene, IFN-ind – the ... lymphomas In this group of upregulated genes there were the a-myb oncogene, the interleukin receptor gene (IL4R), the gene of the transporter protein TAP2, the gene of protease calpain small subunit...

Ngày tải lên: 02/11/2012, 11:08

... using Cell Quest software (BD) Results Gene expression changes were identified by comparing gene expression profiles across the indicated age groups to the gene expression profiles of the month age ... 100 changed genes Red represents up-regulation of gene expression, and green represents down regulation of gene expression http://www.medsci.org Int J Med Sci 2009, 56 Table Top genes that increased ... due to already low levels of expression Up -regulated immunoglobulin-associated genes were easily confirmed and validated the array results for that group of genes GENE NAME kappa light chain of...

Ngày tải lên: 03/11/2012, 11:35

... effect on gene expression and affected the expression of 19 genes, the HIV-1 Tat peptide the expression of 18 genes and the two Yersina-derived delivery systems TP6287 genes and TP6288 genes respectively ... differential gene expression Genes detected and in following investigated for validation A) an induced expression of the PKN1 gene B) a repressed expression of the ZCD1 gene C) a repressed expression ... gene expression patterns, when analyzing the efficacy and the genetic consequences of targeted gene therapy in vitro (and in vivo) Microarray Study Differential Gene Expression Differential gene...

Ngày tải lên: 03/11/2012, 11:44

... plant expression systems  On the basis of compartmentation of recombinant proteins b) By transient expression of genetically engineered viruses 10 Comparative study of plant bioreactor systems GENETIC ... activator of the remaining vir genes Promoters of vir genes possess one of more “vir box” of 12 bp sequence P P AS Vir A P Vir G A mutant that expresses its vir genes constitutively, contains ... because this mutation is compensated by virJ gene (which is absent in nopaline strains) Vir F??? virE genes Both virE1 and E2 are essential for tumorogenesis i.e E1 or E2 mutants are avirulent...

Ngày tải lên: 06/09/2013, 22:46

... possible to interrogate the gene expression patterns in particular cells To investigate which genes are expressed in particular cell types, or which genes are altered in their expression in response ... analysis of gene expression in differentiating neuroblastoma cells Gene 184: 73–81 Kuno N, Muramatsu T, Hamazato F, Furuya M (2000) Identification by largescale screening of phytochrome -regulated genes ... or even at specific intracellular locations showing organellespecific gene expression Principle In situ RT-PCR detects gene expression profiles at the cellular and subcellular level In essence the...

Ngày tải lên: 25/10/2013, 22:20

... (fig 5) Fig 5: Analysis of MCoTI-II gene on 5%polyacrylamide gel Lane 1: DNA Marker 100 bp (100-1031) (#SMO241/2/3) Lane : PCR product of MCoTI-II gene MCoTI-II gene was also successfully prepared ... summarizing the steps in obtaining a construct system for expression of MCoTI-II To identify the insert gene, we isolated plasmid from PI- 17, checking TI gene by PCR technique and sequencing PCR product ... TGAAGCGCCA 480 Fig Sequence of recombinant plasmid DNA containing TI gene fragment Expression of recombinant MCoTI-II (ReMCoTI-II) The expression conditions such as ampicillin and IPTG concentrations,...

Ngày tải lên: 12/02/2014, 10:20