plant expression systems

PLANT SYSTEMS FOR EXPRESSION PLANT SYSTEMS FOR EXPRESSION OF RECOMBINANT PROTEINS OF RECOMBINANT PROTEINS 1 1 Assoc. Prof. Dr. Chu Hoang Ha Plant Cell Biotechnology Department (PCB) Institute of Biotechnology (IBT) A teaching module of “Expression Systems and bioreactions” Unit Hanoi 12/2011 Why plant systems are used ? Why plant systems are used ?  Cheaper than mammalian, insect, or prokaryotic Cheaper than mammalian, insect, or prokaryotic systems systems  Agricultural infrastructure already in place Agricultural infrastructure already in place  Easy to scale-up Easy to scale-up  No human pathogens, endotoxins in plants No human pathogens, endotoxins in plants  Correct folding, modifications Correct folding, modifications  High volume High volume  Direct use Direct use 2 2 4 4 What kind of plants are What kind of plants are used as host? used as host?  Transgenic plants Transgenic plants including including Cereal plants Cereal plants Fruit plants Fruit plants Leguminous plants Leguminous plants Vegetable plants Vegetable plants 6 6 “ “ High level expression is essential High level expression is essential for economic recombinant protein for economic recombinant protein production” production”  Offset costs of production, purification Offset costs of production, purification  Compete with other sources Compete with other sources  Ensure efficacy Ensure efficacy To date… • Few small-scale products have reached market • Not produced economically on large-scale 8 8 Types of plant expression systems Types of plant expression systems  On the basis of compartmentation of recombinant proteins On the basis of compartmentation of recombinant proteins b) By transient expression of genetically engineered viruses 10 10 . Fruit plants Fruit plants Leguminous plants Leguminous plants Vegetable plants Vegetable plants 6 6 “ “ High level expression is essential High level expression. PLANT SYSTEMS FOR EXPRESSION PLANT SYSTEMS FOR EXPRESSION OF RECOMBINANT PROTEINS OF RECOMBINANT PROTEINS 1 1 Assoc. Prof. Dr. Chu Hoang Ha Plant