— lentiviral or bacterial vector expression systems

... Transformation Vectors ‘Disarmed vectors’: non-tumor inducing Ti Plasmids Co-integrative Binary vectors Old Agrobacterium binary vectors: low copy New Binary vectors: high copy Ti plasmid binary vector ... cytoplasm and they contain ATPase activity Therefore, they may be involved in providing energy for the export of other protein subunits, for T-strand transport or both VirB proteins constitute a pilus ... plant expression systems  On the basis of compartmentation of recombinant proteins b) By transient expression of genetically engineered viruses 10 Comparative study of plant bioreactor systems...

Ngày tải lên: 06/09/2013, 22:46

Ngày tải lên: 21/02/2014, 20:20

... Hanson, M., Brorson, K., Moreira, A & Rao, G (2009) Comparisons of optically monitored smallscale stirred tank vessels to optically controlled disposable bag bioreactors Microbial Cell Factories, 8, ... novel Wave Bioreactor® cellbag for aerobic yeast cultivation Bioprocess and Biosystems Engineering, 30, 231-241 Park, H., An, S & Choe, T (2006) Change of insulin-like growth factor gene expression ... Twyman, 2006b) Bioreactor  Stirred tank The number of reactor types has been increased However, because of the simplicity and the ease of documentation, in comparison to other bioreactors, the conventional...

Ngày tải lên: 28/03/2014, 09:20

... GSK-3 binds to both its unphosphorylated and its phoshorylated forms The amount of phosphorylated GSK-3, however, is much lower than the unphosphorylated form and therefore is not detected on the ... was inserted into a pTRE vector from Clontech Laboratories, Inc (Paolo Alto, CA) PC-12 tet-off cells from Clontech Laboratories, Inc., stably transfected with ‘regulator plasmid’, were next transfected ... degradation rate [14–16] Therefore, phosphorylation may represent a major mechanism for regulating RCAN1 Remarkably, RCAN1 can be phosphorylated by GSK-3b and dephosphorylated by calcineurin [13,16]...

Ngày tải lên: 30/03/2014, 11:20

... Hanson, M., Brorson, K., Moreira, A & Rao, G (2009) Comparisons of optically monitored smallscale stirred tank vessels to optically controlled disposable bag bioreactors Microbial Cell Factories, 8, ... novel Wave Bioreactor® cellbag for aerobic yeast cultivation Bioprocess and Biosystems Engineering, 30, 231-241 Park, H., An, S & Choe, T (2006) Change of insulin-like growth factor gene expression ... Twyman, 2006b) Bioreactor  Stirred tank The number of reactor types has been increased However, because of the simplicity and the ease of documentation, in comparison to other bioreactors, the conventional...

Ngày tải lên: 28/06/2014, 12:52

... Mopeia Old World Old World Old World Old World +/++ ++ ++ +/++ ++ + - + +/- ++ ++ + +++ ++ ++ Latino Tamiami Pirital Pichinde Oliveros New World New World New World New World New World +/+ + +/- ... produced in bacterial cell lines using the MBP fusion-based pMAL -vector system (New England BioLabs, Ipswich, MA), comprised of pMAL-p2x and -c2x bacterial expression vectors The former plasmid ... Cloning LASV genes for expression in bacterial cell systems Figure summarizes the strategy used to clone LASV GP1, GP2, and NP gene sequences into vectors pMAL-p2x and -c2x for expression in bacteria...

Ngày tải lên: 20/06/2014, 01:20

... Rev-dependent indicator lentiviral vector (Fig 2A and 2C) In Fig 2C, in order to determine whether a single reporter vector was adequately robust, we added one infectious indicator vector per five cells ... supported Both HIV and the reporter vector use the LTR promoter We also examined high doses of reporter viral vector, where a majority of the cells are infected with the Revdependent reporter vector ... possess more than one reporter provirus Expression of GFP reporter from the HIV-positive cells closely approximated the input reporter viral vector level (Fig and 3), suggesting that undetected expression...

Ngày tải lên: 13/08/2014, 09:20

... predecessor SX-6 3.5 Parallel Pipeline Processing Substantial effort has been made to provide significant vector performance for short vector lengths The crossover between scalar and vector performance ... processors consists of vector add/shift, vector multiply, vector logical and vector divide pipelines The vector divide pipeline which also supports vector square root, generates results every ... peak performance of or 4.4 GFLOPS and the scalar performance of or 2.2 GFLOPS, each processor can achieve a maximum CPU performance of 22 or 24.2 GFLOPS, respectively The vector processor contains...

Ngày tải lên: 15/12/2013, 13:15

... (or nodes) Because the code was originally developed for high-performance vector computers such as CRAY, NEC or Fujitsu, it achieves high vectorization ratios (> 99.8%) In the context of vectorization, ... being a factor of approximately three more complicated In the case of X1 the number of vector registers available for a vectorized loop is more limited than for the SX systems and for complex ... performance is in average almost one order of magnitude higher for the vector machine, clearly demonstrating that the vectors still provide a class of their own if application performance for vectorizable...

Ngày tải lên: 24/12/2013, 19:15

... The algorithm requires memory space to store six vectors, i.e., the residual vector wi , the search direction vector pi , and the eigenvector xi , moreover, Wi , Pi , and Xi Thus, the memory usage ... multiplications, vector load, and store operations, respectively According to the formula (3), the performance of the matrix multiplications AV and V AT , described in the previous section is normally ... Simulator The ES is basically classified in a cluster of SMP’s which are interconnected by a high speed network switch, and each node comprises eight vector PE’s In order to achieve high performance...

Ngày tải lên: 24/12/2013, 19:15

... performance on vector systems The reason for short vector lengths in this case is the sparse storage format used Most of the sparse linear algebra libraries implement either a row oriented or ... especially on vector architectures, where its performance is mainly affected by the average vector length and the frequency of indirect addressing 3.1 Sparse Storage Formats Short vector length is ... for performance only on cache based machines, hence they not performance well on vector systems The main reason for this is the storage formats used in these packages, which are mostly row or...

Ngày tải lên: 24/12/2013, 19:15

... processor, whereas for the search algorithm, the number of donor cells for interpolation must be balanced This code improvement may become important, if in future more processors are used for simulations ... cos(ΨM R ) for the main rotor and ΘT R = 8.0◦ for the tail rotor ΨM R is the azimuth angle of the main rotor Information on the flapping and elastic blade deformation of the main rotor were not ... for Complete Helicopter 135 Performance on NEC SX-8 Vector Computer In spring 2005 the next generation vector computer NEC SX-8 was installed as the successor of the NEC SX-6 at the High Performance...

Ngày tải lên: 24/12/2013, 19:15

... microprocessors and vector computers VASP performs excellently on vector machines, whereas IMD is better suited for large clusters of microprocessors We also report on our efforts to make IMD perform ... limits in the foreseeable future Order N algorithms, which are being studied for insulators, not seem to be applicable to metal systems For many simulation problems, however, systems with a few ... list each time is an order N operation, and is not feasible for large systems For moderately big systems, Verlet neighbor lists are often used The idea is to construct for each atom a list of...

Ngày tải lên: 24/12/2013, 19:15

... Kremer, Vectorized Link Cell Fortran Code for Molecu ular Dynamics Simulations for a Large Number of Particles, Comp Phys Comm 55 (1989) 269–285 R Everaers, K Kremer, A fast grid search algorithm for ... memory layout for the vector version, in which the particle data is stored in single big arrays and not in per-cell arrays The cells then contain only indices into the big particle list In order ... Regatta (bottom) systems Classical Molecular Dynamics on the NEC SX The algorithm for the force computation sketched in Sect 3.1 suffers from two problems, when executed on vector computers The...

Ngày tải lên: 24/12/2013, 19:15

... factor data for plane channel turbulence Dotted line: Blasius formula (4) for pipe flow in coeff Dashed line: Blasius formula modified for plane channel flow, with CB = 0.0685 Solid line: Prandtl formula ... single-time autoand cross-correlations (or their corresponding Fourier spectra, see [20] and its corresponding references) reveals the existence of very long streamwise correlations Corresponding very ... problems while keeping a high vectorization for favourable computing performance The flow chosen for first computations is a mixing layer which may serve as a model flow for the initial part of a jet...

Ngày tải lên: 24/12/2013, 19:15

... Performance Good computational performance of a parallel code is first of all based on its single processor performance As the NEC SX-8 is a vector computer we use its characteristic values for ... efficiency of 89.3% for four processors with the theoretical value of 78.1% according to Eq (29) shows that even for 2-d computations, solving the tridiagonal equation system is not the major part of computation ... 83% for processes A somehow strange behaviour is the fact that the efficiency of the single processor run is less than one Therefore efficiency is based on the maximum performance per processor The...

Ngày tải lên: 24/12/2013, 19:15

... Cisco Systems, Inc All rights reserved ICND v2. 0—5 -19 Distance Vector Operation © 2002, Cisco Systems, Inc All rights reserved ICND v2. 0—5 -20 Distance Vector Operation (Cont.) © 2002, Cisco Systems, ... rights reserved ICND v2. 0—5 -21 Distance Vector Operation (Cont.) © 2002, Cisco Systems, Inc All rights reserved ICND v2. 0—5 -22 Distance Vector Operation (Cont.) © 2002, Cisco Systems, Inc All rights ... rights reserved ICND v2. 0—5 -23 Distance Vector Operation (Cont.) © 2002, Cisco Systems, Inc All rights reserved ICND v2. 0—5 -24 Distance Vector Operation (Cont.) © 2002, Cisco Systems, Inc All rights...

Ngày tải lên: 17/02/2014, 08:20

... pCIneo-EGFP vector (EGFP vector) or the vector encoding httEx1-25Q-EGFP (25Q EGFP vector) , httEx1-72Q-EGFP (72Q EGFP vector) or httEx1-103Q-EGFP (103Q EGFP vector) Cotransfections were performed using ... pCIneoEGFP vector (EGFP vector) or the vector encoding httEx172Q EGFP (72Q EGFP) In addition, cotransfections were performed using vectors encoding Hsp70 ⁄ Hdj-1, Hsp70 ⁄ Hdj-2, Hsp27 or mutant ... pCIneo-EGFP vector (EGFP vector) , or the same vector encoding httEx1-25Q-EGFP (25Q EGFP), httEx1-72Q-EGFP (72Q EGFP) or httEx1-103Q-EGFP (103Q EGFP) Cotransfections were performed using the vectors encoding...

Ngày tải lên: 19/02/2014, 06:20

... the NcoI/ BamHI site of the bacterial expression vector pET 26b (Novagen, Madison, WI) E coli DH5a cells were transformed with ligation products for amplification Expression was carried out in ... with or without DTT and boiled for before electrophoresis Proteins bands were visualized using Coommassie Brilliant Blue SAgs were also analyzed by immunoblotting using an anti-His mAb (Sigma) or ... Sekaly, University of Montreal, Canada), were cloned between the NdeI and EcoRI restriction sites of the pET17b expression vector and expressed in E coli BL21(DE3) as inclusion bodies Glycerol stocks...

Ngày tải lên: 07/03/2014, 16:20

... After incubation of the cells for 30 at °C, mM phenylmethanesulfonyl ¯uoride and mM dithiothreitol were added and the cells were sonicated for 10 in a bath sonicator Bacterial supernatant was then ... proteins in more complex systems ACKNOWLEDGEMENTS We thank Dr Brian Golding of Biology Department, McMaster University, for assisting with analysis of the human Rab32 gene This work was supported by ... immunoreactive species corresponding to human Rab32 was found in rat platelets or in any rat tissue examined 266 X Bao et al (Eur J Biochem 269) (Fig 6) Instead, an equivalent amount of an immunoreactive...

Ngày tải lên: 08/03/2014, 16:20