episomal vs integrated expression of cloned genes

... Sub-network view Often researchers are interested in the regulatory interactions between a group of genes and miRNAs For example, given a set of candidate genes (for example, genes mutated in ... sets of predicted direct and indirect targets of these transcription factors EEDB also provides users with a more integrated view of how individual genes are regulated, both at the level of alternative ... EEDBnumbers ineach of at the time of sets Summary forinaccessiondata storedEEDB centric and anand subAdditionalof offile viewkeyEEDB infor promoters, viewand edge views 2current the raw of EEDB EEDB...

Ngày tải lên: 14/08/2014, 21:20

... transcript level of the hupTUV genes is below the detection limit or is missing in T roseopersicina Mutagenesis and homologous expression of the hupT and hupTUV genes In-frame deletion mutagenesis was ... specific hydrogenase assay of the membrane fraction Deletion of the hynSL genes did not affect the activity of HupSL hydrogenase [11] Mutation in the structural genes of both membrane bound hydrogenases ... hupUV mutation changed the expression of hupS::lacZ (Table 2) The hupT gene (pTrTUVC2), hupUV genes (pMHEUVC2) or hupTUV genes (pTrTUVC1) were cloned behind the promoter of the crtD gene and expressed...

Ngày tải lên: 16/03/2014, 23:20

... baculovirus expression system has become one of the major players in the field of protein expression, reflected in the vast number of published examples of the successful production of secreted, ... ANALYSIS OF GENE EXPRESSION [2] lished in 1981 by Y Gluzman, have found widespread application for the rapid analysis of functionality of plasmids and for expression cloning and production of recombinant ... extrachromosomal replication of plasmids carrying an SV40 origin of replication 38 On transfection of an expression plasmid featuring the gene of interest under the control of a strong, mostly viral,...

Ngày tải lên: 11/04/2014, 09:41

... of reporter gene expression seen post transduction may be a reflection of a number of variables including transduction efficiency, number of copies of integrated transgene, and efficiencies of ... efficiency of coexpression of genes, K562 and Hela cells were transduced at various MOI First Table 2: Relative expression of MGMT and eGFP Vector Relative MGMT Expression Relative eGFP Expression ... inconsistent levels of expression of individual transgenes within the expression cassette From our data summarized in Table 2, it is clear that the relative levels of MGMT and eGFP expression from...

Ngày tải lên: 19/06/2014, 08:20

... of reporter gene expression seen post transduction may be a reflection of a number of variables including transduction efficiency, number of copies of integrated transgene, and efficiencies of ... efficiency of coexpression of genes, K562 and Hela cells were transduced at various MOI First Table 2: Relative expression of MGMT and eGFP Vector Relative MGMT Expression Relative eGFP Expression ... inconsistent levels of expression of individual transgenes within the expression cassette From our data summarized in Table 2, it is clear that the relative levels of MGMT and eGFP expression from...

Ngày tải lên: 20/06/2014, 04:20

... The use of fenofibrate was related to significant decreases in the expression in circulating leukocytes of CX3CL1 and CCR2 genes and a significant increase in the expression of SDF-1 Page of (page ... the expression of chemokine receptors CX3CR1 and CXCR4 but the expression of CCR2 was significantly decreased in patients treated with fenofibrate (U-Mann-Whitney, p = 0.016; Figure 1B) Of note, ... significant effect of fenofibrate in the MCP-1/CCL2 gene expression However, there was a significant decrease in the gene expression of the CX3CL1/ Fractalkine in patients treated with fenofibrate with...

Ngày tải lên: 10/08/2014, 05:21

... bursa of chickens are poorly described and their role in the pathogenesis of such infections has not yet been extensively studied Of the seven genes examined in this study, the levels of expression ... the expression levels of IFN-g and IL-12p40 genes (Figure 2A and 2E) in bursal tissues were significantly upregulated compared with uninfected birds (P < 0.05) and the expression of IL-2 genes ... the course of the Ts infection (P > 0.05) (Figure 2D) Th2-cytokine expression during IBDV infection Temporal expression patterns of IL-4, IL-5, IL-13 and IL-10 genes in the bursa of chickens...

Ngày tải lên: 11/08/2014, 21:21

... graph of genes from GpII…………………………… 119 4-22 An expression graph of genes from GpIII………………………… 120 4-23 An expression graph of genes from GpIV………………………… 121 4-24 An expression graph of genes ... of statistically significant genes from the t-test… 116 4-19 Expression graph of significant genes from t-test………………… 117 4-20 An expression graph of genes from GpI…………………………… 118 4-21 An expression ... the conventional methods of expression analyses of one or two genes per experiment Large-scale analysis of the genome enables the elucidation of the expression patterns of the whole genome in a...

Ngày tải lên: 04/10/2015, 15:58

... that some EBV genes may cause methylation of some promoters of cellular genes, including the promoters of tumour suppressor genes, and results in down-regulation of the expression of these tumour ... preferentially activating expression of some but not all intronless genes (Ruvolo et al., 2001) Except regulation of viral gene expression, SM protein can increase expression of some cell genes, such as ... of EBV gene expression in NPC The aims of this study are: To construct a reliable profiling system which can detect the RNA expression of all EBV genes To comprehensively study the EBV gene expression...

Ngày tải lên: 05/10/2015, 22:32

... excess of kinetic energy (as a measure of temperature) and its effect on enzyme activity Alternatively, we know of a number of examples of qualitative strategies, for example, when expression of ... of the LDH gene has been found Sequence divergence between pairs of isoforms, known in diploid teleosts is $ 20–25% (for ORFs of mlc1 and mlc2 genes) , while divergence between the two forms of ... confirmed the existence of two LDH-A mRNAs of different lengths: short (a-isoform; b mRNAa ldhÀa ¼ 1332 bp) and long (b-isoform; mRNAldhÀa ¼ 1550 bp) Sequence analysis of these mRNAs has shown...

Ngày tải lên: 19/02/2014, 02:20

... expression of prp3 The observed differential developmental expression patterns of these three genes should help clarify the functional relationships of duplicated forms of the prion-related genes ... organization of structural prion protein motifs The developmental expression profiles of prp1 and prp2 were determined FEBS Journal 272 (2005) 500–513 ª 2004 FEBS Expression of prion genes in the ... 2004 FEBS E Cotto et al Expression of prion genes in the developing zebrafish Fig Comparison between prp2 and prp3 developmental expression patterns in zebrafish The expression of prp2 (A,C) and prp3...

Ngày tải lên: 19/02/2014, 16:20

... physiological transcriptional repressor of the expression of LMO2 and HGAL genes This inhibitory effect may mediate the loss of HGAL and LMO2 expression upon differentiation of GC B cells to plasma cells ... of transfection mix (1 lL of SiPort, 75 ng of 3072 pRL-TK, 1.25 lg of the luciferase reporter plasmid, and lg of the PRDM1a expression plasmid or control pCDNA3.1 per well) in a final volume of ... by PRDM1 Results PRDM1 overexpression suppresses endogenous LMO2 and HGAL expression Gene expression profiling previously performed by us [2] reveals that expression of HGAL and LMO2 is FEBS Journal...

Ngày tải lên: 05/03/2014, 23:20

... without a lag period; expression was already signicantly different from that of the control after 0.5 h of recovery (P < 0.05) A peak of Hsp40 expression was observed after h of recovery Hsp40 is ... recovery phase This study provides an overview of the temporal expression of D melanogaster Hsp genes in response to cold stress As the network of Hsp genes clearly shows species specicity [61], ... coefcients of variation ranged from 10% to 13% for stress duration of 0.256 h, and then increased to 28% for h of cold stress As often observed in animals, the phenotypic variability of a trait...

Ngày tải lên: 06/03/2014, 09:22

... effects on the expression of 12 genes and opposite effects on the regulation of eight genes These results suggest that rapamycin inhibition of TORC1 modifies the expression of a set of genes that ... GCN2-independent regulation of gene expression Our results show that rapamycin, an inhibitor of mTORC1, regulates the expression of a set of genes almost as large as the set of genes regulated by amino ... behavior of gene expression on rapamycin treatment in MEF cells (A) Number of genes exhibiting changes in their expression level after h of rapamycin treatment (50 nM) Filled bars, expression...

Ngày tải lên: 07/03/2014, 03:20

... have been unsuccessful because of loss of synthetic activity during rupture of plasmodia in the preparation of extracts [15] The PMLA synthetic capacity of plasmodia of the yellow strains such as ... suppression of PMLA synthesis Among other possibilities, this effect on PMLA synthesis could be the result of loss of induction at the transcriptional level, of loss of activation of the synthetase ... Pallotta D (1988) Expression of the three unlinked isocoding actin genes of Physarum polycephalum DNA 7, 317–328 34 Kroneder R, Cashmore AR & Marwan W (1999) Phytochrome-induced expression of lig1, a...

Ngày tải lên: 07/03/2014, 12:20

... expression system for molybdenum hydroxylases harboring the MCD cofactor, we tested whether expression of the qorMSL genes from P putida 86 in P putida host strains results in the formation of ... the genes that code for Qor might be a suitable host for the expression cloning of genes coding for Mo–MCD-containing molybdenum hydroxylases By replacing two copies of qorMSL in Fig Synthesis of ... g-factor of Ó FEBS 2003 Expression of quinoline 2-oxidoreductase genes (Eur J Biochem 270) 1575 in P putida KT2440 and in a qorMSL deletion mutant of P putida 86-1 results in the formation of catalytically...

Ngày tải lên: 17/03/2014, 10:20

... constituent of membrane, we examined whether glutamine regulates the expression of genes involved in cholesterol homeostasis To investigate the effect of glutamine on the transcription of genes related ... in the induction of SREBP-1a gene expression When endogenous Sp1 expression was reduced to  20% of normal with gene-specific small interfering RNA (siRNA), the gene expression of SREBP-1a was ... that the basal gene expression of SREBP-1a is under the control of Sp1 Moreover, the elevation in the mRNA level of SREBP-1a by glutamine was abolished by the knockdown of Sp1 expression (Fig 3E)...

Ngày tải lên: 28/03/2014, 22:21

... steroidogenic gene expression upon inactivation of the T-antigen To examine effects of inactivation of the T-antigen on expression of the genes for steroidogenesis, we cultured AcA101, AcA201, and AcE60 ... our cell lines showed inducible expression of 3bHSD and P450c21 genes indicate that the cell lines have the property of adrenocortical cells Especially, expression of the endogenous P450c21 gene ... consistent with the expression levels of mRNA molecules of the steroidogenic genes, which were regulated through the protein kinase A pathway Accumulation of pregnenolone and low levels of progesterone,...

Ngày tải lên: 31/03/2014, 15:20

... time toprevent accumulation ofPPO mRNA The early expression ofendogenous PPO genes during organogenesis, taken together withthe longhalf-life of thePPO antisense PPO genes These results were further ... Construction of T-DNA vectors carrying antisense PPO cDNAs In order to maximize the chances ofachieving ahigh level of antisense inhibition of PPO gene expression, we designed a series of antisense ... 5'-8O0 bpsection ofboth classes of PPO genes We used the CaMV 35S promoter which gives high expression levels throughout the plant16, as well as two pro moters which direct expression more specifically...

Ngày tải lên: 18/05/2014, 20:10

... 0HWK\OFHOOXORVH Figure Effects of pre-transfection treatment of methylcellulose on expression of the PV L1 genes in mouse primary KCs Effects of pre-transfection treatment of methylcellulose on expression ... 0HWK\OFHOOXORVH % 0RG / / ,QYR 7XE 1DW / / ,QYR 7XE 0HWK\OFHOOXORVH Figure Effects of post-transfection treatment of methylcellulose on expression of PV L1 genes in mouse primary KCs Effects of ... treatment of methylcellulose also differentially regulated expression of the L1 proteins from PV Nat or Mod L1 genes, confirming further that expression of the L1 proteins from PV Nat and Mod L1 genes...

Ngày tải lên: 18/06/2014, 18:20