discovery of the stem cell apos s unique abilities

... Metabolic Diseases Another group of diseases being treated with HSC transplantation is the lysosomal storage disorders, including the mucopolysaccharidoses such as Hurler s syndrome These diseases involve ... However, studies on model systems have revealed a surprisingly large number of other ways in which stem cell therapy may provide benefits Transplanted stem cells 37 38 Therapeutic Uses of Stem Cells ... chapters of the volume are devoted to the politics of stem cell research Larry Goldstein discusses stem cell politics prior to the passage of Proposition 71, the California Stem Cell Research...

Ngày tải lên: 11/06/2014, 12:52

... the statistical SPSS 13.0 software The paired-sample t-testwas used to test the probability of significant differences between samples Statistical significance was defined as p < 0.05 Results ... Page of 10 Figure Effect of MSCs on T cell apoptosis Flk-1+CD31-CD34MSCs at 1:10 ratios (MSCs to T cells); the data are expressed as mean ± S. D of triplicates of five separate experiments with similar ... T cell cycle Flk-1+CD31-CD34- MSCs or 3T3 at 1:10 ratios (MSCs to T cells); the data are expressed as mean ± S. D Of triplicates of five separate experiments with similar results Cell cycles of...

Ngày tải lên: 10/08/2014, 10:21

... stem/ progenitor cell compartments [16], we asked whether increased expression of SALL4 isoforms may affect HSC/HPC phenotypes To study this, mouse HSC/HPC s (Lin-, Sca-1 +, c-Kit +; LSK cells) ... Sall4B isoforms act as potent regulators of HSC/HPC self-renewal Results Sall4 isoforms enhance and support murine LSK cell proliferation Given the selective expression pattern of SALL4 proteins in ... Interestingly, Sall4 is one of the few genes that are also involved in adult tissue stem cells [17,18], and its protein expression is always correlated with the presence of stem and progenitor cell...

Ngày tải lên: 10/08/2014, 21:23

... localizing the basal proteins 1.5 Stem cells and cancer – the cancer stem cell hypothesis The traditional view of cancer has long been associated with the accumulation of mutations that impairs the cells’ ... fact, cell lineage analysis of mutant brain tissues of lgl and brat that were implanted into adult hosts supports the Cancer Stem Cell hypothesis It has been shown that resulting tumors from these ... cells of same cell fate or developmental potential whereas asymmetric cell division generates two daughter cells of distinct cell fates and/or sizes The latter is the one of the most important aspect...

Ngày tải lên: 11/09/2015, 14:32

... Lefty2 shRNA constructs were used to ensure reproducibility and specificity of the silencing effect 54 The changes in expression of key pluripotency genes were also assayed to assess the loss of ... colleagues to further narrow down the choice of candidate genes This thesis documents only the candidate genes for which I was solely responsible for the cloning of their shRNAs and measurements of ... Several criteria were used in the selection of genes The first criterion was the expression level of the candidate genes in pluripotent cell types of both mouse and human, such as in ES cells...

Ngày tải lên: 11/09/2015, 16:02

... of embryonic stem cell fate Also, this thesis extends the current understanding of ES cell self renewal mechanism beyond the established roles of LIF/STAT3, BMP and WNT pathways and that of the ... development and stem cell- specific expression of Lefty has been observed in mouse and human, its functional significance in embryonic stem cells has never been demonstrated A part of this thesis is hence ... addressing if Lefty2 plays any functional role (s) in embryonic stem cells To elucidate the biological role (s) of Lefty2, loss of function studies using shRNAs was carried out I was able to show...

Ngày tải lên: 11/09/2015, 16:02

... (Gibco) selection was introduced 24 hour post transfection to select for cells transfected simultaneously with both the shRNA and the shRNA resistant overexpression construct for three days The samples ... Briefly, 100nM of siRNA was used for each transfection of 2.5 × 105cells in suspension, and subsequently plated onto a 12-well tissue culture plate in the presence of MEFs Retransfection was performed ... supplemented with 10% heatinactivated FBS and penicillin–streptomycin 4.2 RNAi design and construction of plasmids for shRNA synthesis For RNAi design and construction of plasmids for shRNA synthesis,...

Ngày tải lên: 11/09/2015, 16:02

... LIF/STAT3 signaling fails to maintain self-renewal of human embryonic stem cells Stem Cells 22, 770-778 Darr, H., Mayshar, Y., and Benvenisty, N (2006) Overexpression of NANOG in human ES cells ... Hayek, A (2005) Activin A maintains pluripotency of human embryonic stem cells in the absence of feeder layers Stem Cells 23, 489-495 Besser, D (2004) Expression of nodal, lefty-a, and lefty-B in ... undifferentiated state of mouse embryonic stem cells Embo J 18, 4261-4269 Mee, P J., O'Brien, C M., Thomson, H., van der Sar, S. , Lakics, V., and Allsopp, T E (2006) Embryonic stem cells as a source of differentiated...

Ngày tải lên: 11/09/2015, 16:02

... while the mesoderm gives rise to the bones, muscles and most of the cardiac and circulatory system Endoderm gives rise to the respiratory organs and gastrointestinal tract Cells of the ICM also ... its effect being short term (Sato et al., 2004) Expressed sequence tags (EST) scan analysis of human ES cells and massively parallel signature sequencing (MPSS) analysis of mouse and human cells ... to support ES cells This further supports the fact that LIF is a major determinant of the ability of feeders to support ES cell self renewal Today, most investigators culture mouse ES cells feeder...

Ngày tải lên: 11/09/2015, 16:02

... in mouse Es cells LeftyB (human Lefty1) is regulated by Wnt pathway High expression in ES cells and ICM Differential expression pattern between ES cells and differentiated cells Co-expressed with ... tabulation of qPCR data For all the qPCR data presented in this thesis, the relative transcript levels for the Ct method gene of interest between test sample and control were tabulated using the -Actin ... harvested and assayed for Pitx2 transcript level using qPCR analysis The samples were normalized to endogenous -actin and mean values are plotted as percentages relative to non-treated ES cells...

Ngày tải lên: 11/09/2015, 16:03

... ACKNOWLEDGMENTS I would like to express my deepest appreciation to my three supervisors, Assistant Professor He Beiping, Associate Professor Lu Jia and Associate Professor Samuel Sam Wah Tay, Department of ... neurodegenerative diseases Neurodegenerative diseases such as Parkinson s diseases (PD), Alzheimer s diseases (AD), and amyotrophic lateral sclerosis (ALS) are main causes of disability, dementia, ... entire cell undergoes lysis with the resultant spillage of intracellular contents into the surrounding tissues The spillage of lytic enzymes could cause damage to the surrounding tissues and may...

Ngày tải lên: 12/09/2015, 08:19

... of the stars of the Southern Cross These various types of nebulae and star clusters recur throughout our story of the discovery of the Milky Way galaxy, as clues and signposts to the seven astronomers ... gives us the impression that all the stars in a single constellation are at about the same distance from us In some cases, it is true: the stars of the Pleiades the ‘‘seven sisters’’ asterism—are ... night, when the observer is on the shaded side of the Earth The constellation Sagittarius is in the line of sight to the Sun, behind the Sun on the celestial sphere The stars of Sagittarius would...

Ngày tải lên: 19/01/2014, 13:40

... company So, to make a sort of drawn battle of the matter, it was settled that Heinel should be put into an open boat, that lay on the sea-shore hard by; that the father should push him off with his ... saying he set out and travelled till he came to a hill, where three giants were sharing their father s goods; and as they saw him pass they cried out and said, ‘Little men have sharp wits; he shall ... pleased; and a pair of boots that carried the wearer wherever he wished Heinel said they must first let him try these wonderful things, then he might know how to set a value upon them Then they...

Ngày tải lên: 21/01/2014, 03:20

... tends to the boisterous Expert slavehunters, they mostly clothe themselves by the sale of their victims on the coast, though they business by the sale of goats and grain as well Nowhere in the ... famines; yet such is too often the case, and the negro, with these bounties within his reach, is sometimes found eating dogs, cats, rats, porcupines, snakes, lizards, tortoises, locusts, and ... strange as they are themselves It is based on euphony, from which cause it is very complex, the more especially so as it requires one to be possessed of a negro 's turn of mind to appreciate the system,...

Ngày tải lên: 15/02/2014, 09:20

... systems Navy ships also have sources of high and increasing costs that are unique compared with other weapon systems and commercial ships Much of their high cost lies in the fact that the design ... comparisons across specific classes also allows greater transparency of the contribution of individual components to ship cost escalation The ship types and pairs (and fiscal years) of comparison classes ... U .S ships and other weapon systems In sum, cost escalation appears to be a systemic problem for all weapon systems, and not one limited to construction of U .S Navy ships Historical Cost Escalation...

Ngày tải lên: 17/02/2014, 22:20

... comprises all systems such as avionics (electronics to control the aircraft), sensors (such as radar), other mission systems (such as electronic countermeasures, communications, targeting systems, ... aircraft The issues we address are: How does escalation in aircraft costs compare with cost escalation in other sectors of the economy? What are the sources of any escalation in these costs? Can cost ... characteristics have contributed to aircraft cost escalation We present views of some industry representatives on the sources of cost escalation in Chapter Six Next, we discuss some options for addressing...

Ngày tải lên: 17/02/2014, 23:20

... Discharge Permit Systems.51 Under these different systems, permits have been issued in increasing numbers In 1996, over forty thousand permits were issued in the cities that implemented a system.52 ... local standards for items not covered.84 The development of these standards is similar to the standards in the United States The EPA sets treatment standards by regulation according to industry.85 ... these numbers may seem promising, the number of permits issued lags behind the number of polluting industries In some places, only twenty percent of polluting enterprises had permits.54 In other...

Ngày tải lên: 18/02/2014, 04:20

... activities of GS ring-size analogs The amino acid sequences and the 3D structures of the three ring-size analogs of GS studied here are presented in Fig These three peptides are all based on the structure ... was made to increase the water solubility of these compounds and the latter to decrease the cost of chemical synthesis [4–7] Note that these conservative amino acid substitutions not by themselves ... discussion of the thermotropic phase behavior of Myr2Gro-PGro and other members of the homologous series of linear saturated PGs, see Zhang et al [24] The addition of mol percent of one of the...

Ngày tải lên: 21/02/2014, 01:21

... make The rest of the titles of the "Wealth of the People" series are listed at the end of this book Introduction The second book of the series "The Wealth of the People" is about the relationship ... function of the business enterprise to produce wealth for its customers, its owners, and its employees The Wealth of the State The fifth book discusses what happens when we remove the assumption ... his obligations to the satisfaction of the other When trade stops, there will be the loss of income associated with the advantage of the trade relationship The specialization of labor occurs...

Ngày tải lên: 22/02/2014, 12:20

... peculiarity of the top surface Currently this surface is modified by surface washout process - Slopes on granites: there are many steep slopes on the west of the massive, developed along fissures, tectonic ... slope and less than 800m on the western slope Generally, the area topography has large slopes and is strongly dissected The results of soil profile analyses show a mechanical composition of medium ... faults cutting into granites The origin of these surfaces are rapid gravity processes, including falls and collapses, the slope of this surface is greater than 25o, vertical in places Straight profiled,...

Ngày tải lên: 05/03/2014, 16:20