density of cell binding sites affects adhesion and migration
Tài liệu Báo cáo khoa học: Secondary substrate binding strongly affects activity and binding affinity of Bacillus subtilis and Aspergillus niger GH11 xylanases docx
Ngày tải lên :
14/02/2014, 19:20
... existence of these SBS was recently identified by NMR-monitored titrations of Bacillus circulans xylanase [13] and X-ray analysis of crystals of catalytically incompetent mutants of the xylanases of ... the secondary binding sites of the A niger xylanase (A) and the B subtilis xylanase (B) The diagrams of protein–ligand interactions were generated using LIGPLOT [37] based on Vandermarliere et ... engineering of the SBS Residues of the SBS of both XBS and XAN involved in substrate interaction were selected based on the crystal structures of XBS and XAN soaked with xylotetraose and xylopentaose,...
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Báo cáo khoa học: DG-based prediction and experimental confirmation of SYCRP1-binding sites on the Synechocystis genome pot
Ngày tải lên :
30/03/2014, 10:20
... DG-based prediction of DNA binding sites by SYCRP1 Table Putative binding sites of SYCRP1 and the downstream genes The standard errors were calculated from standard errors of DDG No.a Locusb Productb ... search c Position of the center of the putative binding sites relative to the ORF start position d A Sequences of putative binding sites e Changes in binding free energy and standard errors (DDGtotal ... involved in specific binding of SYCRP1 that had little effect on the binding of E coli CRP For prediction of binding sites of SYCRP1 in the genome sequence, the total changes in binding A free energy...
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báo cáo khoa học: " Three-dimensional reconstruction of cell nuclei, internalized quantum dots and sites of lipid peroxidation" docx
Ngày tải lên :
11/08/2014, 00:22
... images are often not provided, owing to the lack of simple and user-friendly software Quantitative data reflecting changes in nuclear shape and volume before, during and after cell insults and pharmacological ... 1440 µm3 Page of 19 (page number not for citation purposes) Journal of Nanobiotechnology 2006, 4:10 Figure 3-D model of the nucleus of a PC12 cell 3-D model of the nucleus of a PC12 cell The translucent ... the use of two different approaches, depending on the nature of the images and of the structures to be segmented Figure 3-D model of the MCF-7 cell shown in Fig 3-D model of the MCF-7 cell shown...
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Báo cáo khoa học: Analysis of DNA-binding sites on Mhr1, a yeast mitochondrial ATP-independent homologous pairing protein potx
Ngày tải lên :
06/03/2014, 09:22
... the identification and characterization of the DNA -binding regions and the binding modes of each of these molecules In this study, we analyzed the Mhr1 sites involved in ssDNA binding, using fluorescence ... DNA -binding sites of Mhr1 Table DNA -binding activity of the Mhr1 variants N, DNA -binding activity is comparable to that of the wild type; +, DNA -binding activity is slightly stronger than that of ... 16 ssDNA (µM) 1441 DNA -binding sites of Mhr1 T Masuda et al protein; Fig 1C) These results imply that the environment of some of the Trp residues changed after the binding of Mhr1 to the 74-mer...
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Báo cáo khoa học: A hybrid clustering of protein binding sites ppt
Ngày tải lên :
15/03/2014, 10:20
... our binding site database contains numerous small clusters l Effect of OPTICS cut-off level Increasing the cutoff level decreases the quality of clustering, and also the number of binding sites ... hybrid clustering of protein binding sites The costs of aligned binding and nonbinding amino acids were as follows: l The cost of two aligned, different amino acids is unity l The cost of aligned, ... normalization Insertion of ‘-’ to the end of the sequence: Insertion of ‘-’ between the first and last binding amino acids of the sequence: gp l Insertion of a one-letter code of a binding amino acid:...
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Báo cáo hóa học: "Molecular Dynamics Simulations of the Roller Nanoimprint Process: Adhesion and Other Mechanical Characteristics" pot
Ngày tải lên :
21/06/2014, 20:20
... MD simulation of the RNIL process The objectives of this study are to understand the deformation behavior of imprinted film and the effect of adhesion and friction with changes of the roller tooth ... displacement of 0.002° per time step The unit of time step was 10-15 s The characteristic width and height of the roller tooth were both nm The roller was composed of 11294 atoms The width and height of ... temperature and c and d were simulated at a temperature of 400 K and 500 K, respectively simulations based on tight -binding many body potential The results showed that the imprint force and adhesion...
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Báo cáo y học: " PARalyzer: definition of RNA binding sites from PAR-CLIP short-read sequence data" ppt
Ngày tải lên :
09/08/2014, 23:20
... in terms of the presence of putative binding motifs normalized to the total size of the identified interaction sites Initial analysis of PAR-CLIP data revealed that interaction sites of different ... matches to the binding motif of the assayed factor However, the majority of RBPs not have known binding motifs Furthermore, only a subset of miRNAs are expressed in any given cell type and available ... the use of 4SU nucleoside analogue in the original PAR-CLIP protocol, the ‘U’ content of an actual binding site and its vicinity will obviously impact the identification of RBP binding sites If...
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Báo cáo y học: " Antibody microarray analysis of cell surface antigens on CD4+ and CD8+ T cells from HIV+ individuals correlates with disease stages" ppt
Ngày tải lên :
13/08/2014, 05:22
... patterns of antibody binding for CD4+ T cells Composite dot scan patterns of antibody binding for CD4+ T cells Half of a duplicate array was shown with the alignment dots "A" at left, top and bottom ... patterns of antibody binding for CD8+ T cells Composite dot scan patterns of antibody binding for CD8+ T cells Half of a duplicate array was shown with the alignment dots "A" at left, top and bottom ... the use of this technology will facilitate further investigation of the causes and control of HIV disease progression and eventually lead to a better understanding of the pathogenesis of the disease...
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Báo cáo y học: "Evaluation of the sensitization rates and identification of IgE-binding components in wild and genetically modified potatoes in patients with allergic disorders" ppt
Ngày tải lên :
13/08/2014, 13:22
... from 0–88%, with 57 and 58% (13 of 23 and 11 of 19, respectively) having a 2+ reaction, 64 and 58% (18 of 28 and 18 of 31, respectively) with a 3+ reaction, 63 and 88% (5 of and of 8, respectively) ... Northern Society of Allergology [12] An A/H of 0.1–1 and an erythema diameter of 21 mm was assigned a reactivity of 2+ An A/H ... 88% (5 of and of 8, respectively) having a 4+ reaction, and 50 and 60% (2 of and of 2, respectively) with a 5+ reaction, and 50% (0 and of 2, respectively) with a 6+ reaction (Table 2, Fig 1)...
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Báo cáo y học: "Association of mannose-binding lectin-2 genotype and serum levels with prognosis of sepsis" doc
Ngày tải lên :
13/08/2014, 19:20
... polymorphisms in MBL2 and the serum concentration of MBL, and assessed whether these polymorphisms influence the severity and prognosis of sepsis in a Korean population Materials and methods Study ... MBL2 genotypes The HH, HL, and LL genotypes of the -550 polymorphism and the AA, AB, and BB genotypes of the codon 54 polymorphism were correlated with high, medium, and low MBL levels, respectively, ... on the frequency of each haplotype versus all others combined between cases and controls Results Demographics of the subjects The characteristics of the patients at the time of admission are...
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Computational modeling of cell signaling dynamics hypothesis management and parameter estimation methods applied to the akt pathway
Ngày tải lên :
09/09/2015, 10:18
... SDS-PAGE and Western blot analysis were used to detect the phosphorylation level of Akt, total level of Akt, and total level of PDK1 Cells were washed once with cold 1x PBS and collected in PBS The cell ... (defined by the scale and offset values) for all experiments Total cell lysate data were offset and normalized to have a highest value of 10 Membrane fractionation data were offset and normalized such ... technologies and biological questions, of different model abstraction levels, and of different disciplines of science First, biologist collaborators were often unfamiliar with the use of computational...
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Mapping of the binding surface between EPHA5 and antagonist peptide by NMR spectroscopy
Ngày tải lên :
16/10/2015, 15:38
... study of these Eph/ephrins will help us to understand the detailed mechanism of the binding and recognition between Eph receptors and ephrin ligands The structures of the ligand -binding domains of ... VII LIST OF FIGURES Figure Domain structure and binding interfaces of Eph receptors and ephrins Figure Structural comparisons of EphA4 and other Eph ligand -binding domains Figure Ephrin binding ... of the ligand binding domains of EphA5 with EphA2 and EphB2 .71 Table 15 N, 15NH and 13C chemical shift of EphA5 at pH 6.3 and 25°C 72 Table 15 N, 15NH and 13C chemical shift of...
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Báo cáo khoa học: Altered expression of tumor protein D52 regulates apoptosis and migration of prostate cancer cells potx
Ngày tải lên :
30/03/2014, 02:20
... overexpression of TPD52 and (B) cell proliferation after downregulation of TPD52 Viability of cells was measured in a colorimetric MTT assay by the detection of formazan formation The proliferation of control ... promotion of metastasis and the genes responsible for prevention of metastasis were downregulated [19] From our cell migration assays, we found that overexpression of TPD52 in LNCaP cells promotes cell ... extracellular matrix proteins and control functions such as adhesion, migration, differentiation, proliferation, survival and motility [37] Usually, integrins avb3 and avb5 are involved in cell migration...
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báo cáo khoa học: " RNAi-mediated knockdown of cyclooxygenase2 inhibits the growth, invasion and migration of SaOS2 human osteosarcoma cells: a case control study" ppt
Ngày tải lên :
10/08/2014, 10:21
... invasion and migration ability of SaOS2 cells Matrix invasion and migration abilities of cancer cells are associated closely with metastatic potential The in vitro cell invasion and migration ... the LV-Control and SaOS2 cells, while levels of VEGFB and VEGFC had no significant changes Figure Measurement of invasion and migration of SaOS2 cells (A) Invading and migrating cells were stained ... SaOS2 cells compared with the LV-Control and parental SaOS2 cells (Figure 2c), which indicated the efficacy and specificity of LV-COX-2siRNA-1 Effects of LV-COX-2siRNA-1 on cell growth of SaOS2 cells...
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Tài liệu Báo cáo Y học: Interaction of decorin with CNBr peptides from collagens I and II Evidence for multiple binding sites and essential lysyl residues in collagen pot
Ngày tải lên :
21/02/2014, 15:20
... multiple binding sites for decorin are present in these collagens We have also tested the influence on decorin binding of chemical modification of Lys and Hyl side chains of collagens and peptides ... spectra of a recombinant decorin purified in the absence of chaotropic agents, with the exception of the wavelength of the minimum (215–216 instead of 218 nm) and very different to the spectrum of ... analysis of the melting transition of modified peptide trimers will be described elsewhere Binding of decorin to collagenous samples and effect of chemical modifications Equimolecular amounts of collagen...
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Báo cáo khoa học: Ion-binding properties of Calnuc, Ca2+ versus Mg2+ – Calnuc adopts additional and unusual Ca2+-binding sites upon interaction with G-protein pdf
Ngày tải lên :
07/03/2014, 00:20
... Ca2+ -binding sites is known to generate the J-band (610–650 nm) and ⁄ or the c-band (480–510 nm) Upon binding to Calnuc, the dye displayed prominent J-band and c-band in both absorption and CD ... J-band and the c-band regions (Fig 6B) on binding to the protein CD results confirmed the absorbance data: Ca2+ binding is able to attenuate both the J-band and the c-band, whereas Mg2+ binding affects ... EF-hands (as shown by the disappearance of both the c-band and the J-band) Magnesium ions on the other hand, behave differently, and seem to have specific affinity for only one of the binding sites...
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Báo cáo khoa học: Expression of the Drosophila melanogaster ATP synthase a subunit gene is regulated by a transcriptional element containing GAF and Adf-1 binding sites pptx
Ngày tải lên :
07/03/2014, 16:20
... footprinting of the 5¢ upstream region of the a-F1-ATPase gene and found that the combination of GAF and Adf-1 binding sites is present both in other Drosophila species (D yakuba and D pseudoobscura) and ... SD of at least three independent experiments Wild type and mutated GAGA and Adf-1 binding sites are shown by open and filled symbols, respectively increase of promoter activity independent of ... assessed Wild type and mutated GAGA and Adf-1 binding sites are shown by open and filled symbols, respectively (C) Activation of the a-F1-ATPase promoter was induced in presence of GAF Different a-F1-ATPase...
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Báo cáo khoa học: Characterization of a cathepsin L-associated protein in Artemia and its relationship to the FAS-I family of cell adhesion proteins pot
Ngày tải lên :
07/03/2014, 16:20
... stability of CL to temperatures and pH normally inconsistent with CL activity Primary sequence analysis of CLAP and cDNA clones coding for CLAP show it to be a cell adhesion protein and member of the ... intermolecular and homotypic adhesion Thus CLAP, through its Fas I domains, may promote docking and stabilization of CL at various extracellular sites The resistance of mature CLAP to destruction by CL and ... isoform each (pI 5.9 for e and pI 6.1 for f), while two bands of pI 5.95 and pI 6.0 were common to each of the major CLAP fractions, although this does not mean that these are identical isoforms...
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Báo cáo khoa học: Carbohydrate binding sites in Candida albicans exo-b-1,3-glucanase and the role of the Phe-Phe ‘clamp’ at the active site entrance ppt
Ngày tải lên :
15/03/2014, 23:20
... Carbohydrate binding sites in Candida exoglucanase Fig Key properties of the +1 subsite of Exg (A) Subsite binding energies based on published kinetic data for Exg-catalysed hydrolysis of b-1,3-linked ... Carbohydrate binding sites in Candida exoglucanase W M Patrick et al Fig Structural consequences of mutations at the Phe-Phe clamp of the +1 subsite Disposition of F144 (lower) and F258 (upper) ... Carbohydrate binding sites in Candida exoglucanase W M Patrick et al to help explain their importance to the specificity and catalytic efficiency of the enzyme The role of the Phe-Phe entranceway Fig Binding...
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Báo cáo khoa học: Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation pptx
Ngày tải lên :
16/03/2014, 10:20
... conventional expression in insect cells Ligand binding of cell- free endothelin B receptor A Single-particle analysis of CF-produced ETBcHx The quality of CF-expressed and purified ETBcHx was analyzed ... the coelution approach gives good evidence for a ligand -binding activity of CF-produced ETB 3262 Fig Ligand binding of ETB derivatives Binding of ETB derivatives to bET-1 was analyzed by pull-down ... derivative as a ligand, have therefore most likely resulted in modified binding kinetics The localization of ligand -binding sites in ETB is still a subject of controversy Labeling of ETB with radioactive...
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