decay processing and polyadenylation in escherichia coli and other prokaryotes
Tài liệu Báo cáo " synthesis, cloning and expression in escherichia coli of a gene coding for Mcoti-ii " ppt
Ngày tải lên :
12/02/2014, 10:20
... it and purified following IMPACT-CN system manufactures instruction by using chitin beads column chromatography The obtained purified recombinant MCoTI-II showing inhibitory activity against ... the 58 kD protein band was found in induced sample as the major band, acounted for 80% of total protein (lane 1,3,5 and 6) 58 kD Fig 10 SDS PAGE pattern of PI-17 proteins Mc Standard molecular ... Standard molecular markers 2, Uninduced 1, 3, 5, Induced (containing 58kD fusion protein band) Cells were broken by sonication in 10mM Tris-HCl buffer pH8 containing 1mM EDTA (TE buffer) After...
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Báo cáo khoa học: Connection of transport and sensing by UhpC, the sensor for external glucose-6-phosphate in Escherichia coli ppt
Ngày tải lên :
08/03/2014, 08:20
... locked in the inducing conformation and which argues against the transport of Glc6P causing an inducing conformation For UhpC and other membrane proteins acting as sensors it has been shown that insertional ... arginine residues Arginine residues in proteins are excellent candidates for the binding of negatively charged substrates like Glc6P [18] Maloney and coworkers showed that two of 14 arginine ... functional distinction between both proteins is difficult Fig clearly illustrates that UhpC proteins and UhpT proteins substantially similar Arginine 204 is present in all UhpC proteins and in the Glc6P...
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Báo cáo Y học: Overexpression of a recombinant wild-type and His-tagged Bacillus subtilis glycine oxidase in Escherichia coli pptx
Ngày tải lên :
08/03/2014, 16:20
... glycine, glycine-ester and D-pipecolic acid was only observed using GO Interestingly, D-proline is the only amino acid in which the a-amino group is involved in a covalent bond with the side chain ... native PAGE in the presence of: (1) sarcosine; (2) glycine; (3) N-ethylglycine; (4) N,N-dimethylglycine; (5) D-alanine; (6) L-alanine; (7) D-proline; (8) L-proline; (9) D-valine; (10) L-valine, as ... (monomeric and tetrameric sarcosine oxidases and sarcosine and dimethylglycine dehydrogenases), on D-amino acids (DAAO and DASPO) and on pipecolic acid (PIPOX) (see Table 1) The metabolic role of GO in...
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Báo cáo khoa học: Esculentin-1b(1–18) – a membrane-active antimicrobial peptide that synergizes with antibiotics and modifies the expression level of a limited number of proteins in Escherichia coli doc
Ngày tải lên :
16/03/2014, 00:20
... peptide, and dermaseptin, from frog skin [11]; (c) drosocin and pyrrhocoricin, from insects [12]; and (d) Bac-7(1–35), corresponding to the 35-residue N-terminal region of Bac-7 from bovine neutrophils ... (lgÆmL)1) FIC Ampicillin Carbenicillin Cephalosporin C Ceftazidime Chloramphenicol Erythromycin Imipenem Nalidixic acid Netilmicin Oxacillin Rifampicin Streptomycin Tazocin Tetracycline 64 128 0.5 32 ... the underlying molecular mechanism, we analyzed the effect(s) of this peptide on Escherichia coli ATCC 25922 by investigating the following: (a) its microbicidal action and kinetics in different...
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Báo cáo Y học: Oxidation of propionate to pyruvate in Escherichia coli Involvement of methylcitrate dehydratase and aconitase pot
Ngày tải lên :
17/03/2014, 10:20
... containing plasmids with the corresponding genes and an N-terminal cloned histidine tag For purification of wild-type enzymes and expression studies, cells were grown aerobically at 37 °C in minimal ... 5.68 35.7 Galactose-binding protein MalE 5.22 40.7 Maltose-binding protein Proteins induced at a lower level as compared with growth on acetate: AdhP 5.94 35.4 Propanol-preferring alcohol dehydrogenase ... which is specifically induced by iron and redox stress [29] Interestingly, two enzymes are involved in the conversion of methylcitrate into 2-methylisocitrate PrpD is involved in the dehydration...
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Báo cáo Y học: DNA supercoiling in Escherichia coli is under tight and subtle homeostatic control, involving gene-expression and metabolic regulation of both topoisomerase I and DNA gyrase docx
Ngày tải lên :
18/03/2014, 01:20
... previously [17] Supercoiling assay DNA supercoiling was assessed in terms of the linking number of intracellular plasmid pBR322 [18] DNA supercoiling was expressed as the active linking number, aLk, ... the difference in linking number of pBR322 in the respective sample and of pBR322 isolated from cells incubated for 30 with 0.1 mgámL)1 of coumermycin and 0.2 mgámL)1 rifampicin Topoisomerase ... containing the N-terminal part of topA was inserted This resulted in the plasmid pTOPA2A5TS, in which the pA1lacO-1 promoter and the lacIq1 gene are surrounded by a DNA fragment originating from...
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Báo cáo khoa học: Stabilities and activities of the N- and C-domains of FKBP22 from a psychrotrophic bacterium overproduced in Escherichia coli pptx
Ngày tải lên :
23/03/2014, 13:20
... proteins in soluble and insoluble forms by SDS ⁄ PAGE N-domain+ (A), C-domain– (B), and C-domain+ (C) were overproduced in E coli as described for SIB1 FKBP22* [6] The soluble (lane S) and insoluble ... SIB1 FKBP22* and E coli FKBP22* Fig DSC curves of N-domain+, C-domain+, and SIB1 FKBP22* The DSC curves of N-domain+ (thick line), C-domain+ (thin line), and SIB1 FKBP22* (dashed line), which ... FKBP22* and E coli FKBP22* The CD spectra of SIB1 FKBP22* measured at 10 °C (thick line) and 50 °C (thick dashed line), and those of E coli FKBP22* measured at 20 °C (thin line) and 80 °C (thin dashed...
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Báo cáo Y học: Extra terminal residues have a profound effect on the folding and solubility of a Plasmodium falciparum sexual stage-specific protein over-expressed in Escherichia coli pptx
Ngày tải lên :
23/03/2014, 21:20
... protein However, the behavior of the proteins varied once the cells had been lysed for downstream processing and protein purification Pfg27A produced the highest protein levels in a stable and ... DISCUSSION The routine production of recombinant proteins in soluble and biologically active form remains a challenge In this context, MBP and other fusion protein expression systems have been widely ... repressor protein Ara C Aldehyde dehydrogenase C-terminal tail Increased stability C-terminal tail Increased stability Reference [4] [5] C-terminal tail Increased stability [11] C-terminal tail Increased...
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Báo cáo Y học: Puri®cation and characterization of the human adenosine A2a receptor functionally expressed in Escherichia coli doc
Ngày tải lên :
24/03/2014, 00:21
... maltose-binding protein Binding of the fusion protein to the IMAC gel in buffer containing 50 mM imidazole indicates the presence of the C-terminal histidine tag We conclude that the 65-kDa band observed ... Radioligand binding-analyses on E coli and P pastoris membrane preparations, containing the S2 or the S4 somatostatin receptor fusion protein did not reveal speci®c binding of the agonist somatostatin ... 269) 85 and 12±18 nM in one-point saturation assays Bound and free ligand were separated by gel ®ltration using NICK Spin columns Columns were equilibrated in LBA buffer containing 0.1% DDM and 0.02%...
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Báo cáo khoa học: Translation initiation region dependency of translation initiation in Escherichia coli by IF1 and kasugamycin pdf
Ngày tải lên :
29/03/2014, 09:20
... protein bands were excised and kept overnight for extraction in 300 lL of a solution containing 30% H2O2 and 1% NH4OH at 37 °C The resulting solution was placed in a scintillation vial containing ... kasugamycin-binding site on the 30S subunit or on the 70S ribosome in the region of the mRNA-binding tunnel in the E-site and P-site [32,33] As no effect of kasugamycin on mRNA binding to the ... 3A¢ and 2A¢ genes was used to study protein A¢ expression in the strains by gel scanning Proteins in CVR40D and CVR69 were labeled with [3H]lysine The MG1655 parental strain was labeled during...
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Báo cáo khoa học: Purification and functional characterization of human 11b hydroxylase expressed in Escherichia coli doc
Ngày tải lên :
30/03/2014, 04:20
... hydrogen peroxide, other NADPH consuming reactions probably involving the redox partners AdR and Adx are taking place Further studies will focus on investigating this interesting question Experiments ... was in the range of values determined in previous studies for the interaction between bovine Adx and bCYP11B1 Biacore measurements were performed to investigate the binding behavior between bovine ... solutions in the two syringes were saturated with CO prior to loading into the driving syringes All resulting curves were evaluated using sigmaplot 2001 Kinetic traces were analyzed using monoexponential...
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Báo cáo khoa học: Coexpression, purification and characterization of the E and S subunits of coenzyme B12 and B6 dependent Clostridium sticklandii D-ornithine aminomutase in Escherichia coli potx
Ngày tải lên :
30/03/2014, 15:20
... PLP-binding site, a lysine residue at position 629, at the C-terminus of the OraE protein [11] The binding of PLP to D-ornithine aminomutase was investigated by equilibrium dialysis The proteins in ... role in protein folding during in vivo translation To examine this, a mutant protein, OraSE-K629M, which is unable to bind PLP, was constructed and produced in E coli As the bacterial strain used ... D-ornithine aminomutase is strict Discussion Fig Binding of PLP to recombinant measured by equilibrium dialysis D-ornithine aminomutase The genes encoding D-ornithine aminomutase, oraE and oraS,...
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Báo cáo y học: "Perceiving molecular evolution processes in Escherichia coli by comprehensive metabolite and gene expression profiling" doc
Ngày tải lên :
14/08/2014, 08:21
... Asparagine Threonine Methionine Isoleucine Thymine Pyrimidine Uracil Cis-aconitate Isocitrate α - Ketoglutarate Glutamate family Glutamate Glutamine Arginine Polyamines Proline 5-methyl -thioadenosine ... diversion of key intermediates to biosynthetic pathways In the box and whisker plots, and represent MG and DH lines (ancestors), and and represent MGAdp and DHAdp lines (evolved) The top and bottom ... regarding adaptation processes and selection pressures, thereby explaining modes of evolution In this study we used Escherichia coli K-12 strains (MG1655 and DH10B) subjected to the following processes:...
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Báo cáo y học: "Chromatin architecture and gene expression in Escherichia coli" pdf
Ngày tải lên :
14/08/2014, 14:21
... used in the two studies, however, this picture is expected for the gyrase genes These genes are known to be sensitive to supercoiling and are involved in maintaining a precise level of supercoiling ... supercoiling domains in the E coli chromosome This is a cartoon of the chromosome; in real life there are perhaps as many as 400 different domains Plectonemic (unrestrained) and toroidal (restrained, ... (such as melting of the DNA helix) Thus, if there were regions along the chromosome that contained lots of binding sites for proteins involved in chromatin structure, most of the supercoiling would...
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Báo cáo y học: " Leaner and meaner genomes in Escherichia coli" ppsx
Ngày tải lên :
14/08/2014, 17:22
... aphidicola strain BBp) codes for only 504 proteins, or less than 10% of the number of proteins encoded by the larger E coli genomes (5,379 proteins in E coli CFT073) The smallest ‘normal’, freeliving enterobacter ... deletions engineered by Posfai et al [5] are indicated outside the circles Coding sequences of the reference genome (E coli k-12 strain MG1655) are indicated as blue and red blocks, corresponding to ... mircobiology will join the engineering sciences Acknowledgements The reduced genomes are shown in the inner circles B aphidicola strain BBp is the smallest genome, and is depicted as the orange inner circle,...
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Expression analysis of y TMT AND FAD2 gene in escherichia coli and transgenic trichoderma reesei
Ngày tải lên :
22/05/2016, 11:28
... chilled on ice for 30 and put in 42ºC water bath for The mixture was put immediately on ice again for about min, incubated in LB broth in shaking incubator (160 rpm) for 60 at 37ºC and streaked onto ... time by increasing the initial inoculum Fill six 30 ml Corex tubes with the culture and spin at 10.000 rpm at 4°C Pour off supernatant and drain tubes inverted for 60 seconds Resuspend cells in each ... spin Resuspend cells to each tube in ml of ice cold 10% glycerol Combine and aliquot into tubes Repeat spin Resuspend cells in each of the two tubes in 2ml of ice cold 10% glycerol Combine into...
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Bài thuyết trình về: "Alkali and Halotolerant Catalase from Halomonas sp. SK1: Overexpression in Escherichia coli, Purification, Characterization, and Genetic Modification"
Ngày tải lên :
29/09/2016, 17:10
... Functions in Protein Folding and Proteostasis.Yujin E Kim,∗ Mark S Hipp,∗ Andreas Bracher, Manajit Hayer-Hartl, and F Ulrich Hartl Annu Rev Biochem 2013 82:323–55 This second printing of the ... Iba, Y., Kurosawa, Y., and Maruyama, T., FK506 binding protein from the hyper-thermophilic archaeon Pyrococcus horikoshii suppresses the aggregation of proteins in Escherichia coli Appl.Environ Microbiol ... + Domain đầu C- kéo dài TÀI LIỆU THAM KHẢO Phucharoen, K., Hoshino, K., Takenaka, Y., and Shinozawa, T., Purification, characterization, and gene sequencing of a catalase from an alkali- and halo-tolerant...
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Tài liệu Báo cáo khoa học: Catabolite repression in Escherichia coli – a comparison of modelling approaches docx
Ngày tải lên :
18/02/2014, 13:20
... EIIA and B domains being part of the phosphorylation chain and the EIIC domain representing the membrane domain As all components of the PTS, depending on their phosphorylation status, can interact ... cytoplasmic proteins, enzyme I (EI) and histidine-containing protein (HPr), as well as an array of carbohydrate-specific enzyme II (EII) complexes EII is typically composed of EIIA, B and C domains, with ... glucose was reduced from saturating to limiting concentrations As the model comprises metabolic processes, protein–protein and protein–DNA interactions as well as protein synthesis, it is expected...
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Tài liệu Báo cáo khoa học: Substrate specificity of the pseudouridine synthase RluD in Escherichia coli doc
Ngày tải lên :
18/02/2014, 16:20
... hairpin 35 and its interaction with the tylosin-resistance methyltransferase RlmAII EMBO J 22, 183–192 28 Lindahl L (1975) Intermediates and time kinetics of the in vivo assembly of Escherichia coli ... Chem 227, 907–915 Koonin EV (1996) Pseudouridine synthases: four families of enzymes containing a putative uridine-binding motif also conserved in dUTPases and dCTP deaminases Nucleic Acids Res ... in the H69 region Ribosomal subunit assembly involves folding of rRNA and r-proteins and association of both into functional subunits In addition, post-transcriptional modifications are made in...
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Tài liệu Báo cáo khoa học: Yeast glycogenin (Glg2p) produced in Escherichia coli is simultaneously glucosylated at two vicinal tyrosine residues but results in a reduced bacterial glycogen accumulation docx
Ngày tải lên :
19/02/2014, 16:20
... dominant protein band from the patterns obtained at the beginning (Glg2p0) and from the end of the incubation period (Glg2p20) were excised and digested with trypsin in the gel The resulting ... to post source decay (PSD) analysis Protein in- gel digestion and extraction of peptides Following SDS/PAGE and Coomassie blue staining, protein bands (approximately 7.5 lg protein each) were treated ... recombinant Glg2p The recombinant Glg2p carrying a C-terminal His6 tag was purified close to homogeneity and incubated with UDPglucose At intervals (0, 5, 10, 15 and 20 min), aliquots of the incubation...
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