comparisons of the b components of eacmcv

Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is speciﬁcally induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf

... performed by using the Blast programs [26] at the server of the National Center for Biotechnology Information (Bethesda, Md., USA) Puriﬁcation of b- 1,4-endogalactanase Culture ﬂuid was collected by ... degree of L-arabinose substitution by GALA results in different products Arabinogalactans with a low degree of L-arabinose substitution (potato, onion) as substrates result in the liberation of monomers, ... were stopped by the addition of 650 lL ethanol The precipitated substrate was removed by centrifugation and the supernatant was used to measure absorbance at 590 nm The pH optimum of GALA was...

Ngày tải lên: 21/02/2014, 01:21

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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx

... dimethylation of H3K4 and recruitment of RNA Pol II and TBP to the LCR occurs before activation of any of the globin genes The presence of H3 dimethylated at K4 indicates that these elements are permissible ... and the adult b- globin gene 20 and 30% of CD34+ cells, which are known to express low levels of the adult b- globin gene; this could explain the presence of HS2 and b- globin gene transcripts in these ... to the LCR of the murine b- globin gene locus in MEL cells (A) Schematic representation of the murine b- globin gene locus LCR hypersensitive sites and globin genes are shown as shaded boxes (B) ...

Ngày tải lên: 07/03/2014, 12:20

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Báo cáo khoa học: Puriﬁcation and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf

... H2O The quantitative contributions of the individual amide I¢ component bands, determined by band fitting of the absorbance spectrum of Fig 4A, are shown in Fig and Table From Table 2, the sum of ... Assay of human PKIb activity The activity of purified human PKIb was assayed by the inhibition of the catalytic subunit of cAPK in a 50 lL reaction containing 0.5 units of purified catalytic subunit, ... Da) obtained by electrospray MS was in complete accordance with the theoretical mass of the human PKIb (8468.2 Da) [11] The identification of the gene product of human PKIb excluded it from the...

Ngày tải lên: 07/03/2014, 15:20

6 531 0

Báo cáo khoa học: Discovery of GSK837149A, an inhibitor of human fatty acid synthase targeting the b-ketoacyl reductase reaction pot

... relevance of the inhibitors is still noticeable because of their high-afnity binding to the enzyme and the novelty of their mechanism of action It is conceivable that they could be utilized as probes ... could be rescued from the inhibitory effect of GSK837149A by simply removing the unbound compound by ltration and diluting to reconstitute the initial volume The drop-off in the effect of the compound ... inhibitor of human fatty acid synthase Enzymatic mode of inhibition Table Summary of inhibition patterns caused by GSK837149A on the KR reaction catalyzed by FAS With the aim of nding the mode of...

Ngày tải lên: 16/03/2014, 06:20

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Báo cáo khoa học: Concerted mutation of Phe residues belonging to the b-dystroglycan ectodomain strongly inhibits the interaction with a-dystroglycan in vitro pot

... polyclonal antibody directed against the C-terminal domain of a-DG on intact 293-Ebna cells (B) Detection of b- DG was carried out using b- DG antibody in permeabilized 293-Ebna cells Both subunits of wild-type ... binding between the two DG subunits in vitro, has no evident effect on the plasmalemmal targeting of b- DG (Fig 5B) The correct membrane targeting of DG was also confirmed by the immunodetection of the ... which greatly reduces the affinity of b- DG for the a-subunit in vitro, did not influence the localization of the two DG subunits (Fig 5A ,B) In order to detect any effect of the double mutation Phe692...

Ngày tải lên: 16/03/2014, 12:20

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Báo cáo Y học: The Fe-only nitrogenase and the Mo nitrogenase from Rhodobacter capsulatus A comparative study on the redox properties of the metal clusters present in the dinitrogenase components doc

... that of the P1+ signal (g ¼ 2.06, 1.96, 1.83) of the Rhodobacter MoFe protein Furthermore, the redox potential of the cluster responsible for the g ¼ 2.27 feature is, compared to the P1+ signal of ... with the control spectrum in the absence of Rc1Mo Furthermore, with the exception of the classical S ¼ 3/2 signal of the MoFe protein at lower temperatures (< 12 K), no other signal was detectable ... rhombic but that the resonance in the g ¼ 1.8 region might be too broad to be detectable Because the g ¼ 2.22 signal is narrower than the g ¼ 2.27 signal, the g strain may not completely obscure...

Ngày tải lên: 18/03/2014, 01:20

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Báo cáo khoa học: The b-N-acetylglucosaminidases NAG1 and NAG2 are essential for growth of Trichoderma atroviride on chitin doc

... which the WT and Dnag1Dnag2 strains produced a similar number of spores, while the Dnag2 strains only produced 11 ± 1% of the number of spores produced by the WT and the number of spores in the ... sources by quantification of the numbers of spores formed on agar plates, but again no significant influence of the loss of nag1 and nag2 could be detected The only exception was the carbon source ... into the monomer GlcNAc, which can then be taken up by the hyphae and catabolized The small amount of residual biomass that was formed upon growth of the double knockout strain on chitin was probably...

Ngày tải lên: 23/03/2014, 05:22

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Báo cáo khoa học: Recruitment of coregulator complexes to the b-globin gene locus by TFII-I and upstream stimulatory factor doc

... that the PRC2 complex, or components thereof, participate in the repression of the adult b- globin gene in embryonic erythroid cells We also detected an increase in the expression of the embryonic ... 2.5 lg of a-IgG (as negative control) antibodies The complexes were captured by incubation with anti-(rabbit IgG) beads Complexes were eluted off the beads with Laemmli buffer and incubation ... as outlined The expression of the genes is regulated by a LCR composed of ﬁve DNaseI HS and located approximately 15–27 kbp upstream of the embryonic e-globin gene The murine b- globin gene locus,...

Ngày tải lên: 23/03/2014, 07:20

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Báo cáo khoa học: The phosphatidylethanolamine level of yeast mitochondria is affected by the mitochondrial components Oxa1p and Yme1p ppt

... that these proteins are degraded in the absence of Oxa1p [25] When functional Oxa1p is missing the membrane subunits of these complexes cannot be assembled and are cleaved by the intermembrane ... Recently, Mba1p was identiﬁed as a protein that interacts with the Oxa1p insertion machinery of the IMM [17] Mba1p binds to the large subunit of mitochondrial ribosomes and thereby cooperates with the ... Integrity of RNA was tested by agarose gel electrophoresis and determination of the 260 to 280 nm ratio of the absorbencies RNA concentration was determined by measurement of the absorbance at...

Ngày tải lên: 30/03/2014, 04:20

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Báo cáo khoa học: Expression and characterization of soluble forms of the extracellular domains of the b, c and e subunits of the human muscle acetylcholine receptor pot

... unavailability of a conformation-dependent e subunit mAb, only binding of antiFLAG mAb was tested CD spectra Binding of the rabbit anti -b serum to recombinant b1 –221 and human AChR When the b subunit ... conﬁrming the FPLC data, considering that the ‘height’ of the ECD of the AChR is nm [3] This difference in solubility between the c–e and the b ECDs might be attributed to the primary structure of the ... using the conformationdependent mAbs 73 (binds to an epitope on the extracellular side of the b subunit) [22] and 67 (binds to an epitope on the extracellular side of the c subunit) [22] and the...

Ngày tải lên: 30/03/2014, 10:20

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Báo cáo khoa học: Thioredoxin reductase from the malaria mosquito Anopheles gambiae Comparisons with the orthologous enzymes of Plasmodium falciparum and the human host pdf

... from the nicotinamide of NADPH via the ﬂavin and the pair Cys57/Cys62 to the redox centre Cys500’/Cys501’ of the other subunit, and hence to the disulﬁde of the substrate Trx The thiolate of Cys62 ... degradation of the protein isolated from the major band of the SDS/PAGE gel The minor band of 62 kDa resisted Edman degradation Lack of detectable selenium Genomic organization of the A gambiae TrxR ... kcat of 15.4 s)1, A gambiae Trx-1 is the best substrate of the enzyme but D melanogaster Trx-2, i.e the orthologue of A gambiae Trx-1 in Drosophila, is an almost equally good substrate The Km...

Ngày tải lên: 31/03/2014, 07:20

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Báo cáo Y học: Barley a-amylase Met53 situated at the high-affinity subsite )2 belongs to a substrate binding motif in the bfia loop 2 of the catalytic (b/a)8-barrel and is critical for activity and substrate specificity pot

... Mammal Bacterium Mold Yeast Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Yeast Bacterium ... Archaea Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Plant Plant a-Amylase Barley (AMY1) Barley (AMY2) Rice (2A) Maize Wheat (AMY3) Black ... a-Amylases and other GH-H enzymes have a short substrate glycon binding motif in the middle of the typically 35 residues ba loop in the (b/ a)8-barrel (Table 1) In barley AMY1 (for which the structure...

Ngày tải lên: 31/03/2014, 08:20

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Báo cáo Y học: Refolding of the Escherichia coli expressed extracellular domain of a7 nicotinic acetylcholine receptor Cys116 mutation diminishes aggregation and stabilizes the b structure pdf

... inhibitors Analysis of a-bungarotoxin binding As seen from Fig 3A, the expressed proteins speciﬁcally bind 125I-labelled a-cobratoxin on blots Data on the 125 I-labelled aBgt equilibrium binding ... aqueous buffer allowed us to obtain the monomeric fractions (Fig 5E,F) These fractions bind 125 I-labelled aBgt with the afﬁnities similar to those of the starting proteins (Table 1) Because the ... terms of secondary structure, the monomeric form of the GST–a7m-(1–208) protein resembles the protomer of the AChBP This would justify further efforts to prepare the oligomeric a7 ECD using the obtained...

Ngày tải lên: 31/03/2014, 23:20

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Báo cáo sinh học: " Structural comparisons of the nucleoprotein from three negative strand RNA virus families" pdf

... to 4the (5H+3H) motif The structures of the VSV and the BDV N proteins comThe structures of the VSV and the BDV N proteins compared to the (5H+3H) motif The aligned helices are labeled by black ... C-terminal end of the FLUAV N protein, blue (b) Superposition of a hypothetical structure of the FLUAV N protein with the structure of the VSV N protein The orientation of the Cterminal domain is the same ... RNA binding cavity in the rhabdovirus N protein is mostly hydrophobic, which accommodates the bases that point Figure Comparisons of the VSV and BDV N structures Comparisons of the VSV and BDV...

Ngày tải lên: 18/06/2014, 18:20

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báo cáo hóa học: " Measuring the ICF components of impairment, activity limitation and participation restriction: an item analysis using classical test theory and item response theory" potx

... probably due to the positive skew on many of the Ab-P items The distributions of Ab-I, Ab-A and Ab-P all appeared approximately normal when graphically examined (see Figures 5, and 7) None of the ... exploration of properties of the resultant measures was carried out To examine the validity of the new measures, the correlation with subscales of the criterion variable (SF-36) should be as hypothesised ... to as the Aberdeen IAP measures (Ab-IAP) comprising Ab-I, Ab-A and Ab-P The results for the CTT and IRT analysis are initially reported by construct and then the reliability and validity of final...

Ngày tải lên: 18/06/2014, 18:20

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Báo cáo sinh học: " Comparisons of the M1 genome segments and encoded µ2 proteins of different reovirus isolates" pptx

... from the Nibert and Cashdollar laboratories have been shown to exhibit different factory morphologies based on differences in the microtubule-binding capacities of their µ2 proteins and the presence ... length of 11, and a stringency of (B) Surface probability predictions of the T2J µ2 protein, determined as per Emini et al [73], using DNASTAR The predicted surface probability profiles of T1L ... length (Table 1) Because of the single-base deletion described above, the 3' nontranslated region of the T2J M1 plus strand is only 82 nucleotides in length, compared to 83 for T1L and T3D (Table 1)...

Ngày tải lên: 18/06/2014, 22:20

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Báo cáo hóa học: " Structural comparisons of the nucleoprotein from three negative strand RNA virus families" pot

... to 4the (5H+3H) motif The structures of the VSV and the BDV N proteins comThe structures of the VSV and the BDV N proteins compared to the (5H+3H) motif The aligned helices are labeled by black ... C-terminal end of the FLUAV N protein, blue (b) Superposition of a hypothetical structure of the FLUAV N protein with the structure of the VSV N protein The orientation of the Cterminal domain is the same ... RNA binding cavity in the rhabdovirus N protein is mostly hydrophobic, which accommodates the bases that point Figure Comparisons of the VSV and BDV N structures Comparisons of the VSV and BDV...

Ngày tải lên: 20/06/2014, 01:20

7 292 0

báo cáo hóa học:" Comparisons of the M1 genome segments and encoded µ2 proteins of different reovirus isolates" doc

... from the Nibert and Cashdollar laboratories have been shown to exhibit different factory morphologies based on differences in the microtubule-binding capacities of their µ2 proteins and the presence ... length of 11, and a stringency of (B) Surface probability predictions of the T2J µ2 protein, determined as per Emini et al [73], using DNASTAR The predicted surface probability profiles of T1L ... length (Table 1) Because of the single-base deletion described above, the 3' nontranslated region of the T2J M1 plus strand is only 82 nucleotides in length, compared to 83 for T1L and T3D (Table 1)...

Ngày tải lên: 20/06/2014, 04:20

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The chemical components of detergents and their roles in the washing process

... Bleaching agents Bleaching agents eliminate stubborn stains and ensure hygiene by killing bacteria through a chemical oxidation performed by a peroxygen generator, usually sodium perborate The ... control the level of foam so that the washing machine can operate properly ; perfumes ; corrosion inhibitors, such as sodium silicate Fillers Enable the adjustment of the active matter in the detergent ... on fabrics and on the washing machine’s heating elements - stabilise alkalinity at the correct level throughout the washing process thus giving good soil removal and enabling the other components...

Ngày tải lên: 11/07/2014, 16:10

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Module III Scanning.Scanning - DefinitionScanning is one of the three components of intelligence ppsx

... spoofing the IP address of the “zombie” zombie SYN Packet to port 80 spoofing zombie IP address Attacker Target Zombie IDLE Scan: Step 2.2 (Closed Port) The target will send RST to the “zombie” if ... to the server ( 192.168.1.3 ) via a packet with only the SYN flag set p y g The server replies with a packet with both the SYN and the ACK flag set For the final step, the client responds back ... network p y g • Either for the purpose of attacking them or for network security assessment Vulnerability Scanning • The automated process of proactively identifying vulnerabilities of computing systems...

Ngày tải lên: 31/07/2014, 04:20

74 332 0