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Ngày tải lên: 29/06/2014, 09:20

Ngày tải lên: 05/09/2013, 08:40

... from either a perfect shield of site B afforded by bilirubin, or, other- wise, by an alternative conformation of the bilirubin– bilitranslocase complex, totally missed by antibody B. Cyanidin 3-glucoside ... was not stimulated by ATP (data not shown). Immunoblots of carnation petal membrane fractions Membrane proteins from subcellular fractions of carna- tion petals were separated by SDS ⁄ PAGE and ... – University of Trieste) for the immunization and bleeding of rabbits; to Silvia Zezlina for the affin- ity purification of antibody A from rabbit sera; to Dr Paolo Ermacora and Prof Giorgio Honsell...

Ngày tải lên: 20/02/2014, 01:20

... purification of HbpR itself and has been shown previously not to disturb HbpR-mediated tran- scription activation [19]. SDS ⁄ PAGE of the soluble cell extracts of E. coli BL21 (pHB171) showed a protein of 43 ... harvested by centrifu- gation (5 min, 1575 g, at room temperature) and resuspended in Mops medium [5.5 gặL )1 of Mops free acid, 5.1 gặL )1 of Mops sodium salt, 0.5 g ặ L )1 of NaCl, 1 gặL )1 of NH 4 Cl, 0.06 ... activa- tion of HbpR must proceed differently from that of XylR, a fusion protein between DA-HbpR and the A-domain of XylR was found to be incapable of acti- vating the hbpC promoter (not shown). Hence,...

Ngày tải lên: 07/03/2014, 17:20

... filter. RESULTS Binding of Yb 3+ to apo-hTf The thermodynamics of binding of Yb 3+ to apoTf. Binding of Yb 3+ to apo-Tf was investigated by addition of aliquots of Yb 3+ to a solution of apo-TF in 100 ... concentration of Yb- Tf (Fig. 7C). The  FF decreased to half of its original value when 18 mol equiv of Yb-Tf was incubated with cells in the presence of FITC-Fe 2 -Tf. Therefore part of the FITC-Fe 2 - Tf ... NMR spectrum of apo-Tf in the presence of 10 m M bicarbonate was recorded after addition of Yb 3+ (or Lu 3+ ) Fig. 2. Kinetics of binding of Yb 3+ to apo-Tf. The final concentration of the protein...

Ngày tải lên: 08/03/2014, 09:20

... instead of Azo (Fig. 1). Clear-cut on–off photoregulation of DNA transcription was obtained because of the efficient suppression of T7 RNA polymerase (RNAP) binding to the DM-azo in the trans form. By ... stability of the cis form between Azo and DM-azo. (A) Illustration of the reversible photoisomeriza- tion of Azo and thermal isomerization of cis-Azo. (B) Quantitative calculation of cis-form ... Photoregulation of DNA transcription by using photoresponsive T7 promoters and clarification of its mechanism Xingguo Liang 1 , Ryuji Wakuda 1 , Kenta Fujioka 1 and Hiroyuki Asanuma 1,2 1 Department of Molecular...

Ngày tải lên: 15/03/2014, 10:20

... sequence is located inside the iron box of desA (nucleotides 7–12 of the iron box) , explaining the regulation of desA expression by binding of DmdR1 to the iron box. The same overlapping was found ... the decarboxylation of lysine by a lysine decarboxylase encoded by the desA gene [8,9]. Lysine decarboxylases occur in different Streptomyces species and are involved in the utilization of l-lysine ... Production of desferrioxamine was restored by complementation with the whole wild-type desABCD cluster, but not by desA alone, because of a polar effect of the desA gene replacement on expression of...

Ngày tải lên: 16/03/2014, 11:20

... study of Pem-GIH in the shrimp. Biological assay for vitellogenesis-inhibiting activity of Pem-GIH by dsRNA-mediated functional knockdown To test whether the knockdown of Pem-GIH expres- sion by ... ratio of Vg to actin transcripts in the GIH-depleted background suggested that functional knockdown of Pem-GIH led to the induced expression of Vg in the ovary. Discussion Because of the lack of ... the 5Â-region of Pem-GIH. The negative PCR is indicated by ) ve. The actin transcript of P. monodon was used as an internal control of the amount of RNA template. Each panel shows tissue distribution of...

Ngày tải lên: 23/03/2014, 07:20

... Characterization and regulation of TSSK3 TSSK3 activation is different from its involvement in the activation of PKB. Inhibitors of other protein kin- ases, known to be activated by insulin (like p70S6K, p38, ... the release of radioactivity during the fifth cycle (data not shown). Substitution of Val to Tyr in peptide 2 reconstituted phosphorylation of this peptide by TSSK3 almost to level of peptide 4 ... insulin treatment of cells may also slightly (twofold) enhance PDK1 kinase activity [33]. Therefore, it is thought that the role of PDK1 in the activation of other kinases is governed by its cellular...

Ngày tải lên: 23/03/2014, 11:20

... resource itself and how to evaluate its intrinsic quality. In (Davidov and Rappoport, 2008) we show how to use the resource for a known task of a to- tally different nature, classification of relationships between ... in related work. 6.1 Discovery of Known Relationships To estimate recall of our pattern cluster set, we attempted to estimate whether (at least) a subset of known relationships have corresponding ... clustering methods that as- sign given sets of pairs into several clusters, where each cluster corresponds to one of a known set of re- lationship types. Their classification setting is thus very different...

Ngày tải lên: 23/03/2014, 17:20

... attack by kallikrein generated by activation of PPK as a consequense of vessel injury. The activation of FXII and PPK/HK may start with a local increase in the concentration of Zn 2+ as a result of ... although the activity of FXIIageneratedonthecellsbyactivationofFXIIinthe presence of Zn 2+ was significantly higher than that generated in the absence of Zn 2+ , the fraction of bound FXII that became ... activation of FXII and thereby the entire system [6]. In blood plasma the proteolytic efficiency of the proteinases is attenuated by endogenous inactivation of FXIIa and kallikrein by serine proteinase...

Ngày tải lên: 23/03/2014, 20:22

... degree of accuracy, but only if obtained on the same gel. The density of UCP3 protein (i.e. the intensity of the signal expressed per mg of proteins) was similar in the SS mitochondria of the ... the relative amounts of UCP3 protein and mRNA in the three muscles are comparable. Effect of fasting on UCP3 protein expression We and others have shown that fasting induces upregula- tion of UCP3 mRNA ... consisted of 2 min of denatur- ing at 95 °C, followed by 30 cycles with 95 °Cdenaturing for 1 s, 56 °C annealing for 5 s and 72 °C extension for 16 s. The fluorescent product was detected at the end of...

Ngày tải lên: 24/03/2014, 04:21

... increase in its hydrodynamic radius, upon binding of Ca 2+ [30]. Peptide-binding properties of native rGrp94 and its inhibition by physiological ER ligands Since the original studies by Srivastava ... lM of VSV8 and a global R max (obs) of 27 RUặ(ng proteinặmm )2 ). (C) D ouble-reciprocal plot of the equilibrium-binding isotherms obtained in the absence of ATP ⁄ Ca 2+ (d), in the presence of ... properties of native Grp94, inclu- ding its binding parameters. However, interactions of Grp94 with its two related ER lumenal chaperones Grp78 ⁄ BiP and calreticulin were excluded from this study. By...

Ngày tải lên: 30/03/2014, 11:20

... condition of high PtdCho turnover, the addition of inositol can consume part of the PA pool, permitting that partial repression of INO1 transc ription. Alternatively, the a ddi- tion of inositol ... the inability of Sfh2p to decrease PtdCho turnover to wild-type levels i s responsible for the inab ility of S fh2p to complement the INO1 regulatory defect caused by the absence of Sec14p, we measured ... by the following lines of evidence (Fig. 1), namely that (a) mutations in genes encoding structural enzymes of the cytidine diphosphate choline (CDP-choline) pathway for PtdCho biosynthesis bypass...

Ngày tải lên: 30/03/2014, 15:20

... repre- sentation of the structures of the As-rel genome and mRNAs of As-rel1 and As-rel2. (B) Genome sequence of splicing sites. Note that As-rel1 mRNA is generated by splicing at the sites shown by open arrowheads, ... amounts of transfected DNA were kept constant (1.5 lg) by adding an empty vector (pBIND or pCI-neo-Flag vector). The level of activity was normalized on the basis of the level of activity of control ... was verified by Western blotting of the fusion protein. Preparation of the genome and genomic PCR The genome of the ascidian, H. roretzi, was prepared from muscles. A 5 mL volume of extraction...

Ngày tải lên: 30/03/2014, 20:20

... panel) are shown by bold lines. The concentration of acetonitrile is shown by a dotted line. Arrows indicate the position of substance X. Fig. 7. Sucrose density-gradient analysis of nucleosome ... transglutaminase. Determination of the DCA-binding sites of histone H2B Because the carboxamide group of Gln9 of histone H2B is selectively transamidated with the e-amino group of Lys5 or Lys12 of histone H4 in ... analyzed by MALDI-TOF-MS, and was found to have a relative molecular mass (M r ) of 650.32. This M r value is 17 units less than the sum of the M r of DCA (335.50) and that of a tripeptide, Gly–Gln–Lys...

Ngày tải lên: 31/03/2014, 07:20

... the root of contemporary strains, suggesting that VP0 may have been acquired by a more recent ancestor of the group by recom- bination. Discussion Enteritis is caused by a spectrum of viruses ... of the first stage PCR product, under a touchdown cycling protocol: 95°C, 4 min, followed by 10 cycles of 94°C/30s, 65°C, 30s (temperature decrease of 1°C per cycle), 72°C 1 min, fol- lowed by ... Central Page 1 of 10 (page number not for citation purposes) Virology Journal Open Access Research Identification of a contemporary human parechovirus type 1 by VIDISCA and characterisation of its full...

Ngày tải lên: 20/06/2014, 01:20

Ngày tải lên: 22/06/2014, 02:20

Ngày tải lên: 22/06/2014, 20:20

Ngày tải lên: 21/07/2014, 10:28