... with luteinizing hormone receptor mRNA in the ovary J Biol Chem 280, 42809–42816 Wang L, Nair AK & Menon KMJ (2007) Ribonucleic acid binding protein -mediated regulation of luteinizing hormone receptor ... hormone receptor mRNA from ovary J Biol Chem 279, 14937–14944 Nair AK & Menon KM (2005) Regulation of luteinizing hormone receptor expression: evidence of translational suppression in vitro by a ... Identification of a hormonally regulated luteinizing hormone ⁄ human chorionic gonadotropin receptor mRNA binding protein Increased mRNA binding during receptor down-regulation J Biol Chem 273,...
Ngày tải lên: 16/03/2014, 06:20
... that is regulated by A2A adenosine receptors but not by A3 adenosine receptors [24,25] We infer from these results and previous reports that the involvement of different adenosine receptor subtypes ... receptors [31,32] Pharmacological and molecular approaches have shown that neutrophils, monocytes and macrophages express all four adenosine receptor subtypes Although adenosine A1 receptor activation ... [36-38] Adenosine A2A receptor activation inhibits neutrophil and monocyte oxidative burst, degranulation and release of cytokines and chemokines [39-41] Activation of A2B receptors selectively...
Ngày tải lên: 09/08/2014, 07:20
Báo cáo khoa học: E2A participates in a fine control of pre-mature B-cell apoptosis mediated by B-cell receptor signaling via transcriptional regulation of survivin, IAP2 and caspase-8 genes pot
... DT40 cell line mediated by PMA/ionomycin treatment, but also that their own transcription is not influenced by PMA/ionomycin Therefore, the apoptotic cell death of DT40 mediated by PMA/ionomycin ... mRNA level was decreased gradually by PMA/ionomycin treatment by 24 h, and the caspase-10 mRNA level was increased by h and thereafter decreased dramatically by 24 h Expression of caspase-3 and ... caspase-8 gene expression by PMA/ionomycin treatment in E2A-/To further clarify the molecular mechanism linked to the apoptotic induction of the DT40 cell line cooperatively mediated by E2A and BCR signaling,...
Ngày tải lên: 16/03/2014, 04:20
Báo cáo y học: " Protection from pulmonary ischemia-reperfusion injury by adenosine A2A receptor activation" pdf
... attenuated by A2AAR activation Lung IR injury is attenuated by A2AAR activation Lung vascular permeability was assessed by measuring BSA concentration in BAL fluid (A) Lung edema was assessed by measuring ... anti-inflammatory mechanism after lung injury is mediated by the release of adenosine [13] Adenosine receptors are found on various cell types, and the activation of these receptors often results in suppression ... ATL313 KO IR KO IR + ATL313 Figure activation A2AAR Lung IR-induced alveolar MPO content is attenuated by Lung IR-induced alveolar MPO content is attenuated by A2AAR activation MPO content in BAL...
Ngày tải lên: 12/08/2014, 14:20
báo cáo hóa học:" Microarray analysis of Foxl2 mediated gene regulation in the mouse ovary derived KK1 granulosa cell line: Over-expression of Foxl2 leads to activation of the gonadotropin releasing hormone receptor gene promoter" pptx
... of transcription mediated by binding to AP-1 binding site elements [12] Another group of investigators fused Mad SID to the tetracycline receptor and demonstrated tetracycline mediated gene repression ... FSH: follicle stimulating hormone; NO: nitric oxide; LH: luteinizing hormone; hCG: human chorionic gonadotropin Additional file 10: Potential Foxl2 target genes validated by comparison to data from ... analysis of Foxl2 mediated gene regulation in the mouse ovary derived KK1 granulosa cell line: Over-expression of Foxl2 leads to activation of the gonadotropin releasing hormone receptor gene promoter...
Ngày tải lên: 20/06/2014, 07:20
Tài liệu Báo cáo khoa học: 3T3-L1 adipocyte apoptosis induced by thiazolidinediones is peroxisome proliferator-activated receptor-c-dependent and mediated by the caspase-3-dependent apoptotic pathway doc
... factor peroxisome proliferator-activated receptor- c (PPARc) and induce metabolic changes in adipose tissue [16] The mechanism of insulin sensitization by TZDs has been explored, but is not fully ... apoptosis by using 3T3-L1 adipocytes and rat primary adipocytes Adipocyte apoptosis could be induced by troglitazone, pioglitazone or 15-deoxy-D12,14-prostaglandin J2 (PGJ2), and reversed by GW9662, ... 48 h, and mRNA levels were determined by real-time PCR Results are means ± SDs of three independent experiments *P < 0.05; **P < 0.01 (E) Akt-1 activation by GW6992 3T3-L1 adipocytes were treated...
Ngày tải lên: 16/02/2014, 09:20
Tài liệu Báo cáo khoa học: Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt
... CRP This work was supported by grant no P04A 031 16 from the State Committee for Scientic Research References Busby, S & Ebright, R (1999) Transcription activation by Catabolite Activator Protein ... Allosteric regulation of the cAMP receptor protein Biochim Biophys Acta 2, 117 de Crombrugghe, B., Busby, S & Buc, H (1984) Cyclic AMP receptor protein: role in transcription activation Science 224, 831838 ... mediates changes in the protein conformation It is believed that these changes allow the protein molecule to switch from the low-afnity and nonspecic DNA-binding state to the state characterized by...
Ngày tải lên: 20/02/2014, 23:20
Báo cáo khoa học: Fluorescence quenching and kinetic studies of conformational changes induced by DNA and cAMP binding to cAMP receptor protein from Escherichia coli ppt
... analyzed by the external quenchers by using the FQRS method [21,22] Quenching experiments are especially useful in studying the changes in the conformation of proteins that may be induced by ligand ... CRP conformational changes induced by DNA and cAMP 19 20 21 22 23 24 25 26 27 28 29 30 31 cAMP-induced allosteric changes in T127I, S128A and T127I S128A mutants of cAMP receptor protein from ... component, characterized by the average KSV ẳ 1.69 m)1, is responsible for % 45% of the total emission and has a maximum at 338 nm 1105 CRP conformational changes induced by DNA and cAMP M Tworzydo...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: Activation of the plasma membrane H+-ATPase of Saccharomyces cerevisiae by glucose is mediated by dissociation of the H+-ATPase–acetylated tubulin complex docx
... concentrationdependent activation of H+-ATPase, as measured by its ATP-hydrolyzing capacity, reaching a plateau at 150 lm (Fig 1A) At this concentration, HAT was decreased by 80% Activation of plasma H+-ATPase by ... completely understood The activation of yeast H+-ATPase by glucose is regulated at the transcriptional and post-transcriptional levels [6–10] Glucose increases mRNA synthesis by inducing transcription ... 5743 Dissociation of tubulin–H+-ATPase complex by glucose A N Campetelli et al Table Effect of 2-deoxy-D-glucose on H+-ATPase activation induced by D-glucose Glucose-starved cells suspended in...
Ngày tải lên: 23/03/2014, 11:20
Báo cáo khoa học: Differences in substrate specificities between cysteine protease CPB isoforms of Leishmania mexicana are mediated by a few amino acid changes potx
... were monitored by their absorbance at 220 nm and by fluorescence emission at 420 nm following excitation at 320 nm The molecular mass and purity of synthesized peptides were checked by MALDI-TOF ... was monitored by absorbance at 220 nm and by fluorescence at 420 nm after excitation at 320 nm The Abz-containing fragments were compared with authentic synthetic sequences and/or by amino acid ... was further evidenced by the variations in the kcat/Km values for the hydrolysis of various substrates with modifications at P2¢ position by rH84Y – changing the Ser favoured by rCPB2.8 in each case...
Ngày tải lên: 23/03/2014, 13:20
Báo cáo khoa học: Thyroid hormone induces the expression of 4-1BB and activation of caspases in a thyroid hormone receptor-dependent manner pptx
... Thyroid hormone receptors: multiple forms, multiple possibilities Endcr Rev 14, 184–193 Glass, C.K (1996) Some new twists in the regulation of gene expression by thyroid hormone and retinoic acid receptors ... H.-U (2001) Functional CD137 receptors are expressed by eosinphils from patients with IgE -mediated allergic responses but not by eosinophils from patients with non-IgE -mediated eosinophilic disorders ... T3 induces apoptosis, which is mediated by 4-1BB, and subsequent activation of caspases in the tissues and organs in the body Impairment of cell proliferation by T3 was more profound in FRTL...
Ngày tải lên: 23/03/2014, 18:20
Báo cáo khoa học: Downregulation of protease-activated receptor-1 in human lung fibroblasts is specifically mediated by the prostaglandin E2 receptor EP2 through cAMP elevation and protein kinase A pot
... the receptor responsiveness to PAR-1 activators The regulation occurs in a cAMP- and protein kinase A (PKA)-dependent manner PAR-1 downregulation is mediated exclusively by the EP2 receptor, a receptor ... by the EP2 receptor, a receptor for PGE2, but not by other receptors coupled to cAMP elevation, such as b-adrenergic receptor (AR) and adenosine receptor A2B PGE2-induced reduction of the PAR-1 ... Downregulation of PAR-1 expression in human lung fibroblasts (hLFs) is mediated via the PGE2 receptor EP2, but not by other receptors coupled to cAMP elevation In human lung fibroblasts, PGE2 causes...
Ngày tải lên: 30/03/2014, 04:20
Báo cáo khoa học: [Na+]i-induced c-Fos expression is not mediated by activation of the 5¢-promoter containing known transcriptional elements ppt
... (CRE) activated by [Ca2+] elevation in the cytoplasm and nucleus, respectively [11,12] (Fig 1) These data suggest that c-Fos expression in Na+i-loaded cells might be mediated by activation of the ... reported that, side -by- side with elevation of the [Na+]i ⁄ [K+]i ratio, exposure to ouabain results in the transient activation of ERK1 ⁄ via Ras ⁄ Raf -mediated signaling [13,14,17] ERK -mediated signaling ... not accompanied by loss of [K+]i (Table 2) This finding allows us to speculate that high [K+]i protects the signal transduction machinery from its activation by Na+, i probably by competing for...
Ngày tải lên: 30/03/2014, 08:20
Báo cáo hóa học: " Differential inhibition of human cytomegalovirus (HCMV) by toll-like receptor ligands mediated by interferon-beta in human foreskin fibroblasts and cervical tissue" pdf
... inflammatory changes caused by STI could influence HCMV infection Inflammation in genital tract infections is in many cases caused by the activation of genital tract cells through Toll-like receptor ... stimulate human foreskin fibroblasts (HFF) by measuring IL-8 secretion since IL-8 is secreted by a wide variety of cell types in response to stimulation by TLR ligands [21] HFF secreted IL-8 in ... control ulation There was no IL-8 produced by HFF in response to CpG or LTA (data not shown) Anti-HCMV effect of TLR3 and TLR4 ligands in HFF is mediated by IFNβ Since fibroblasts are known to produce...
Ngày tải lên: 20/06/2014, 01:20
báo cáo hóa học:" Luteinizing hormone-induced Akt phosphorylation and androgen production are modulated by MAP Kinase in bovine theca cells" doc
... recently, the effects of cAMP were generally thought to be mediated by activation of cAMP-dependent PKA, a major cAMP target, followed by phosphorylation of many intracellular targets, such as ... androstenedione production by theca cells in rat [22] and cattle [11] It is possible that LH- As described earlier, H89, a potent and selective inhibitor of PKA, did not affect LH -mediated changes in phosphoAkt, ... theca cells, it remains unknown whether this response is mediated via activation of the PI3K/Akt pathway In this study, we examined whether and by what means LH controls PI3K/Akt signaling and androgen...
Ngày tải lên: 20/06/2014, 07:20
Báo cáo y học: "24-hour changes in circulating prolactin, follicle-stimulating hormone, luteinizing hormone and testosterone in male rats subjected to social isolation" doc
... nature of hormone release for most of the hormones considered [17,18] In this study we measured the daily pattern of plasma prolactin, luteinizing hormone (LH), folliclestimulating hormone (FSH) ... that has a dual function – as a circulating hormone and as a cytokine The prolactin receptor is a member of the cytokine receptor superfamily, linked to activation of signaling pathways that promote ... Freund's adjuvant on 24-hour rhythms in plasma prolactin, growth hormone, thyrotropin, insulin, follicle-stimulating hormone, luteinizing hormone and testosterone in rats Life Sciences 2000, 66:1969-1977...
Ngày tải lên: 10/08/2014, 09:20
CONTRIBUTION OF RANKL REGULATION TO BONE RESORPTION INDUCED BY PTH RECEPTOR ACTIVATION IN OSTEOCYTES
... detect changes in the level of hormones, such as estrogen, androgen, glucocorticoids and parathyroid hormone (PTH) Reduction in the Wnt antagonist sost/sclerostin, expressed by osteocytes, by both ... Ben-awadh CONTRIBUTION OF RANKL REGULATION TO BONE RESORPTION INDUCED BY PTH RECEPTOR ACTIVATION IN OSTEOCYTES PTH increases osteoclasts by upregulating RANKL in cells of the osteoblastic lineage, but ... loading and activation of the receptor for parathyroid hormone (PTHR), leads to increase Wnt signaling This increases osteoblast number resulting in enhanced bone formation PTHR activation in...
Ngày tải lên: 24/08/2014, 09:46
Coupling receptor activation to cytoskeleton changes 2
... was carried by incubating the samples with MuLV RT enzyme (NEB), RNase inhibitor and 0.5mM dNTPs in the provided reaction buffer at 37 0C for 1hr The reaction was stopped via heat inactivation ... performed by Dr Wing Chan 2.23 Transwell Migration COS7 cells were treated with control and ACK SiRNAs for 48hrs inclusive of 24hrs serum-starvation before detachment from culture dishes by trypsin ... RNAi, the ratio of 20pmoles to 3ul of the transfection reagent was used The SiRNAs were synthesized by Invitrogen Cells were treated with control SiRNA and SiRNAs against ACK1, Grb2 and Cdc42 The...
Ngày tải lên: 11/09/2015, 09:57
Tài liệu Báo cáo khoa học: Phosphorylation of cyclin dependent kinase 4 on tyrosine 17 is mediated by Src family kinases pptx
... D3-CDK4-p27kip1 holoenzyme in thyroid epithelial cells: activation by TSH, inhibition by TGFbeta, and phosphorylations of its subunits demonstrated by two-dimensional gel electrophoresis Exp Cell Res ... inhibits cell division by phosphorylating p34cdc2 exclusively on Tyr15 EMBO J 12, 75–85 11 Parker LL & Piwnica-Worms H (1992) Inactivation of the p34cdc2-cyclin B complex by the human WEE1 tyrosine ... increased by incubation with the protein tyrosine phosphatase inhibitor vanadate (Figs 1B and 5C) By contrast, the phosphorylation of CDK1 on Y15 was increased to a much lesser degree by vana-...
Ngày tải lên: 18/02/2014, 18:20
Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc
... produced in one third of neutrophils in response to hormones or local metabolic changes [22] ROS also amplify the immune response by enhancing the receptor signaling cascades of T cells [58] In experimental ... mediators by blocking NF-jB activation in macrophages Mol Pharmacol 69, 1783–1790 16 Laos S, Baeckstrom D & Hansson GC (2006) Inhibition of NF-kappaB activation and chemokine expression by the leukocyte ... reported that NF-jB can be activated by ROS [32,33] produced by a pathway involving 5-LOX ⁄ FLAP [34], NADPH oxidase [35] or PI3K ⁄ Akt [30] During NF-jB activation, phosphorylation of IKKa ⁄...
Ngày tải lên: 19/02/2014, 00:20