cell cycle regulation and transcription

... most cell cycle responses. While each single pocket protein can mediate cell cycle arrest upon cell- cell contact, pRb is the critical mediator of cell cycle arrest upon growth factor deprivation and ... (42) P. Kaldis: Cell Cycle Regulation DOI 10.1007/002/Published online: 24 February 2006 © Springer-Verlag Berlin Heidelberg 2006 The Retinoblastoma Gene Family in Cell Cycle Regulation and Suppression ... suppression and cell mortality. Cell 85:27–37 Serrano M, Lin AW, Mila EM, Beach D, Lowe SW (1997) Oncogenic ras provokes prema- ture cell senescence associated with accumulation of p53 and p16 Ink4a . Cell...

Ngày tải lên: 25/10/2013, 21:20

... Regulated During the Cell Cycle by a CDE ⁄ CHR Tandem Element and is Downregulated by p53 but not by p63 or p73. Cell Cycle 6, 853–862. 37 Yoshida K (2005) Cell- cycle- dependent regulation of the human and mouse ... p53. Cell cycle arrest can be medi- ated by p53 downregulating the transcription of central cell cycle regulators such as cyclin B, Cks1, Cdc2 and Cdc25C. CDE ⁄ CHR-dependent cell cycle- gene transcription ... encoded by cyclin B1 and cyclin B2 genes is Keywords cell cycle; cell cycle genes homology region (CHR); cell cycle- dependent element (CDE); DREAM complex; E2F Correspondence K. Engeland, Molecular...

Ngày tải lên: 16/02/2014, 09:20

... completion of the cell division cycle is significantly extended compared with continually fed cells. Thus, starvation not only blocks cell cycle progression, but causes cells to exit the cycle and enter ... of the regulation of the cell cycle and its relation to cancer, which culminated in the 2001 Nobel Prize being awarded to Leland Hartwell, Tim Hunt, and Paul Nurse. In the investigations of cell cycle ... restriction point of the cell cycle. Cell Cycle 1:103–110 Buchkovich K, Duffy LA, Harlow E (1989) The retinoblastoma protein is phosphorylated during specific phases of the cell cycle. Cell 58:1097–1105 Burdon...

Ngày tải lên: 07/10/2012, 16:51

... the cell cycle process affected: initiation and maintenance of meiosis, transcriptional and translational fac- tors, cellular signaling, cytoplasmic and apoptotic factors and prophase I regulation. 2 Cell ... pregnancy loss and birth defects, it is critical to dissect out mei- otic cell cycle checkpoints and proper chromosome segregation (Hassold and Hunt 2001). However, understanding cell cycle regulation ... kinase, but male and female Cell Cycle Regulation in Mammalian Germ Cells 347 Table 1 (continued) Gene Protein Name Effect on germ cell development Refs. B. Transcriptional and Translational...

Ngày tải lên: 25/10/2013, 21:20

... Nature 343:665–669 Modeling Cell Cycle Control and Cancer with pRB Tumor Suppressor 231 cycle, and strongly suggested that deregulation of the mechanisms controlling cell cycle progression could ... mechanisms for controlling cell cycle progression (Morgan 1997; Zachariae and Nasmyth 1999). These studies and others demonstrated the universality of the cell Modeling Cell Cycle Control and Cancer with ... Orc1 and Mcm2–7) and DNA replication (e.g. Dhfr, Rnr, TK, TS and Polα). Many E2F target genes encode products in- volved in cell cycle progression (e.g. cyclins A and E, pRB, p107, E2F1–3) and apoptosis...

Ngày tải lên: 25/10/2013, 21:20

... REVIEW ARTICLE Cell biology, regulation and inhibition of b-secretase (BACE-1) Clare E. Hunt and Anthony J. Turner Proteolysis Research Group, Institute of Molecular and Cellular Biology, ... 10001–10006. 110 Andrau D, Dumanchin-Njock C, Ayral E, Vizzavona J, Farzan M, Boisbrun M, Fulcrand P, Hernandez JF, Martinez J, Lefranc-Jullien S et al. (2003) BACE1- and BACE2-expressing human cells: ... with isophthalamide derivatives as the P 2 –P 3 ligands showed good selectivity between BACE-1 and BACE-2, nanomolar potency in vitro and in cell- based studies, and a significant reduction in Ab40 levels...

Ngày tải lên: 07/03/2014, 00:20

... i n contrast to their regulation during the cell cycle [13], upon exit and entry to the cell cycle both genes are controlled, at least in part, transcriptionally. Cdt1 and geminin are highly ... upon cell cycle exit. To this effect, HFF cells were deprived of serum f or 48 h to induce cell cycle exit. Serum was th en r e-added a nd samples taken as cells progressed into the cell cycle. ... under r eview, regulation of geminin b y E2F and R B was reported [34,35]. Geminin protein accumulates in HFF cells re-entering the cell cycle from G0 3–6 h later t han Cdt1, and as cells enter...

Ngày tải lên: 07/03/2014, 16:20

Ngày tải lên: 10/04/2014, 11:13

... control of vertebrate cell cycle progression through transcription regulation of various cell cycle- related genes. Gene 347, 83–97. 24 Kikuchi H & Nakayama T (2008) GCN5 and BCR sig- nalling ... on cell- cycle progression and proliferation of DT40 and E2A )/) cells. Cells cultured in the presence of PMA/ ionomycin for 24 h or etoposide for 6 h were analyzed by fluorescence-activated cell ... apoptotic cell death of the DT40 cell line, and show that E2A is involved in fine control of pre-mature B -cell apoptosis mediated by BCR signaling, via transcriptional regula- tion of survivin, IAP2 and...

Ngày tải lên: 16/03/2014, 04:20

... regulation of HLA-DR gene transcription, post transcriptional events and shedding of surface HLA-DR, as well as the regulation of HLA-DR by GM-CSF and an immunomodulatory cytokine. Plasma and ... the cell line, HL-60, was cultured with GM-CSF we demonstrated that an up -regulation of HLA- DR mRNA was observed from 2 hours culture and at time points 4, 6 and 8 hours. Transcriptional regulation ... visualisation of sections through the cell to observe surface and intracellular staining. Figures 3a-d show the intracellular and surface staining of PBMC in a healthy control and from a septic patient....

Ngày tải lên: 03/11/2012, 09:57

... RA (1995) The role of Rb in cell cycle control. Prog Cell Cycle Res 1:9–19 Senescence and Cell Cycle Control 259 eliminates telomere-mediated signaling, allowing cells to undergo transform- ation ... loss of G1 control and immortalization. Genes Dev 14:3037–3050 Senescence and Cell Cycle Control 267 Campisi J (2001) Cellular senescence as a tumor-suppressor mechanism. Trends Cell Biol 11:S27–S31 Carnero ... mouse cells. p19 ARF is predominantly upregulated during senescence by oncogene activation and/ or oxidative stress Senescence and Cell Cycle Control 263 induction by controlling p16 INK4a (and...

Ngày tải lên: 25/10/2013, 21:20

... phase of the cell- division cycle. Therefore, we tested, using light microscopy and after DAPI staining, whether the slow growth yields a cell- cycle phenotype. Cells of the logarithmically ... which is stable throughout the cell cycle and highly resistant to proteolytic degradation [40]. Miscellaneous procedures Yeast were grown on standard media [41] and transformed as described by ... esses, e.g. cell proliferation, signal transduction, transcriptional regulation, transla- tion and metabolism [3]. Despite this eminent role in strictly regulated cellular processes and although...

Ngày tải lên: 18/02/2014, 16:20

... TRAF4 induction in T-cells, we analyzed a mutant Jurkat cell Fig. 9. TNF and PMA up-regulate TRAF4 mRNA in T-cells. Jurkat (A) and D23II-7 T-cells (B) as well as primary human T-cells and human T -cell blasts ... transfected cells were selected for photography (A). For quantification cells showing increased localiza- tion of the proteins in cell cell contacts were counted (B). Ó FEBS 2002 Localization and transcriptional ... form of the respective Fig. 5. Subcellular localization of TRAF4 deletion mutants in cells with cell cell contacts. HeLa cells were seeded on glass cover slides and transiently transfected with...

Ngày tải lên: 21/02/2014, 15:20

... promoter in somatic cells. MATERIALS AND METHODS Cells, plasmids and materials Mouse NIH3T3 cells and Drosophila SL2 cells were obtained from the JCRB Cell Bank (Tokyo, Japan) and from S. Kojima ... nuclear extracts of NIH3T3 cells with the P2 probe. We observed three similar shifted bands when we used nuclear extracts from 3T6 cells, HeLa cells, 293 cells and F9 cells (data not shown). To ... according to the manufacturer’s protocol. Cell culture, transfections and assays of promoter activity Mouse NIH3T3 and F9 cells and human HeLa and 293 cells were grown in Dulbecco’s modified Eagle’s...

Ngày tải lên: 22/02/2014, 07:20

... recent developments in the study of transcription and transcriptional regulation by nuclear actin, and the regulation of muscle-specific gene expression, nuclear receptor and transcription complexes by ... STARS-transfected cells did not differ from those in untransfected cells. Thus, Fig. 2. Model of the involvement of STARS and ABLIM in actin dynamics and SRF-dependent transcription. Actin and ABPs in transcription ... complexes and is necessary for transcription by RNA polymerase II. Nat Cell Biol 6, 1094–1101. 20 Shang Y, Myers M & Brown M (2002) Formation of the androgen receptor transcription complex. Mol Cell 9,...

Ngày tải lên: 07/03/2014, 01:20

... in growth and cell cycle duration. However, we do not know how great an effect should be expected. If we assume that certain PMLA–protein complexes are involved in growth and cell cycle timing, ... proteins to nucleic acids, and it c ould affect cell g rowth a nd cell cycle duration. The distributing activity of PMLA and the efficiency of competition between PMLA and nucleic acids would both ... PMLA induced a strain specific enhancement of cell growth and an equal (between strains) shortening of the cell cycle. Materials and methods Strains and materials The following strains of P. polycephalum...

Ngày tải lên: 16/03/2014, 18:20

Ngày tải lên: 17/03/2014, 23:20

Ngày tải lên: 23/03/2014, 06:20

Ngày tải lên: 23/03/2014, 07:20