cell cycle regulated transcription

... Subunit 1 (Cks1) is Regulated During the Cell Cycle by a CDE ⁄ CHR Tandem Element and is Downregulated by p53 but not by p63 or p73. Cell Cycle 6, 853–862. 37 Yoshida K (2005) Cell- cycle- dependent ... p53. Cell cycle arrest can be medi- ated by p53 downregulating the transcription of central cell cycle regulators such as cyclin B, Cks1, Cdc2 and Cdc25C. CDE ⁄ CHR-dependent cell cycle- gene transcription ... responsible for strong shifts in cell cycle distribu- tion. A large cell fraction is found in G 2 or appears to have an 8n DNA content. Many cell cycle- regulated genes are downregulated upon Lin-9 knockdown...

Ngày tải lên: 16/02/2014, 09:20

... upon cell cycle exit. To this effect, HFF cells were deprived of serum f or 48 h to induce cell cycle exit. Serum was th en r e-added a nd samples taken as cells progressed into the cell cycle. ... proliferating cells in vivo The experiments with cultured cells have suggested that Cdt1 and geminin mRNA and protein are down -regulated upon cell cycle exit and a re progressively up -regulated upon Fig. ... ase, when licensing is legitimate [13]. When cells exit the cell cycle, licensing in lost, a nd is est ablished again a s cells prepare t o reenter the cell cycle. While a previous study did not detect...

Ngày tải lên: 07/03/2014, 16:20

Ngày tải lên: 23/03/2014, 07:20

... restriction point of the cell cycle. Cell Cycle 1:103–110 Buchkovich K, Duffy LA, Harlow E (1989) The retinoblastoma protein is phosphorylated during specific phases of the cell cycle. Cell 58:1097–1105 Burdon ... starvation not only blocks cell cycle progression, but causes cells to exit the cycle and enter G0. However, if serum is removed after this point, cells continue through the cycle unhin- dered (Zetterberg ... of critical cell cycle machinery. 2.3 Cell Cycle Machinery Recognition that specific protein catalysts are responsible for diverse cellu- lar processes such as fermentation (late 1800s) suggested that cell...

Ngày tải lên: 07/10/2012, 16:51

... tangles contain the antigenic profile of many cell cycle markers, re- flecting a re-entry into the cell cycle by affected neurons. However, while such a cell cycle re-entry phenotype is an early and ... suppression of the cell cycle and cell survival instead of apoptosis and their presence may be a neuroprotective factor to prolong the life of the cell after re-entry into the cell cycle, pro- tecting ... dividing neuronal cells during development, and is present in smaller amounts in fully differentiated cells [13]. BRCA1 is known to regulate transcription, regulate cell cycle progression,...

Ngày tải lên: 26/10/2012, 10:04

... induction of cell cycle arrest and apoptosis. We have investigated its effects on cell cycle regulation using a gastric cancer cell line, AGS. The effects of CKBM on cell proliferation, cell cycle ... cell line. CKBM is found to inhibit cell proliferation through the induction of apoptosis and G2/M cell cycle arrest. To further elucidate the mechanism of CKBM on apoptosis and G2/M cell cycle ... AGS cells were seeded in a 6-well plate at a density of 1.2 x 10 6 cells/well. After 2, 4 or 6 hr of CKBM treatment at a concentration of 5, 10 or 15 % v/v, cells were trypsinized. Then cells...

Ngày tải lên: 02/11/2012, 11:12

... female Cell Cycle Regulation in Mammalian Germ Cells 347 Table 1 (continued) Gene Protein Name Effect on germ cell development Refs. B. Transcriptional and Translational Factors Ahch Dax1 A transcriptional ... Probl Cell Differ (42) P. Kaldis: Cell Cycle Regulation DOI 10.1007/003/Published online: 20 December 2005 © Springer-Verlag Berlin Heidelberg 2005 Cell Cycle Regulation in Mammalian Germ Cells Changanamkandath ... stage of cell cycle, Ortega et al. 2003; kinase 2 while females lack follicular development Berthet et al. 2003 due to breakdown at the pachytene stage. CyclinD2 Cell cycle Absence of germ cells...

Ngày tải lên: 25/10/2013, 21:20

... coordinate cell cycle exit with the induction of differentiation that confers upon pRB its tumor suppressor function. 230 L. Yamasaki 2 The Universality of the Cell Cycle The discovery of the cell cycle ... Nature 343:665–669 Modeling Cell Cycle Control and Cancer with pRB Tumor Suppressor 231 cycle, and strongly suggested that deregulation of the mechanisms controlling cell cycle progression could ... PK (2004) Wagging the dogma; tissue-specific cell cycle control in the mouse embryo. Cell 118:535–538 Palmero I, Peters G (1996) Perturbation of cell cycle regulators in human cancer. Cancer Surv...

Ngày tải lên: 25/10/2013, 21:20

... principal cell cycle inhibitor in β cells, as it accumu- latesinthenucleusofβ cells from obese mice, inhibiting compensatory β cell expansion (Uchida et al. 2005). Mouse Models of Cell Cycle Regulators: ... one round of cell division, every cell in metazoans has to decide whether it will re-enter the cell cycle, exit the cell cycle and en- ter a quiescence state, and every quiescent cell has to similarly ... re-enter the cycle. All these crucial decisions are made by a set of information processors (cell cycle machinery) that integrate extracellular and intracellular signals to coordinate cell cycle events. In...

Ngày tải lên: 25/10/2013, 21:20

... RA (1995) The role of Rb in cell cycle control. Prog Cell Cycle Res 1:9–19 Senescence and Cell Cycle Control 259 eliminates telomere-mediated signaling, allowing cells to undergo transform- ation ... of retinoblastoma gene function is sufficient for cell cycle re-entry. Nature 424:223–228 Serrano M, Hannon GJ, Beach D (1993) A new regulatory motif in cell- cycle control causing specific inhibition of ... and cell mortality. Cell 85:27–37 Serrano M, Lin AW, McCurrach ME, Beach D, Lowe SW (1997) Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16 INK4a .Cell 88:593–602 Sharpless...

Ngày tải lên: 25/10/2013, 21:20

... critical regulator of most cell cycle responses. While each single pocket protein can mediate cell cycle arrest upon cell- cell contact, pRb is the critical mediator of cell cycle arrest upon growth ... roles in controlling cell cycle exit upon growth-inhibiting signals. Only ablation of all pocket proteins fully alleviated a cell cycle arrest upon serum withdrawal, cell- cell contact inhi- bition, ... Estes S, Starz M, Vernon JP, Lees JA (1997) E2F activity is regulated by cell cycle- dependent changes in subcellular localization. Mol Cell Biol 17:7268–7282 Vooijs M, Berns A (1999) Developmental...

Ngày tải lên: 25/10/2013, 21:20

... from studies on the early embryonic cell cycles of Table 1 Cell cycle targets of the anaphase promoting complex Substrate Organism Specificity factor Cell cycle function Securin S. cerevisiae ... Probl Cell Differ (42) P. Kaldis: Cell Cycle Regulation DOI 10.1007/b136681/Published online: 6 July 2005 © Springer-Verlag Berlin Heidelberg 2005 The ubiquitin-proteasome pathway in cell cycle ... linking cell cycle regulation to internal and external signaling pathways via regulated protein kinase activities. 1 Introduction The importance of ubiquitin-mediated proteolysis for cell cycle...

Ngày tải lên: 25/10/2013, 21:20

... phase of the cell- division cycle. Therefore, we tested, using light microscopy and after DAPI staining, whether the slow growth yields a cell- cycle phenotype. Cells of the logarithmically ... asynchronous cells. To test whether phosphorylated Rio1p is, in fact, degraded at a certain stage of the cell- division cycle, whereas the unphosphorylated form is not, we per- formed cell- cycle arrest ... for cell life, CK2 activity by itself is apparently unregulated [4,5], although some fluctua- tion in activity in correlation with cell- cycle progres- sion has been seen in cultured mammalian cells...

Ngày tải lên: 18/02/2014, 16:20

... staining cells. Scale bars = 200 lm. (F) Quantification of binucle- ated cells in control and Grx3-KD cells. Total 600 cells counted for control cells and 360 cells counted for Grx3-KD cells. The ... finish cell cycle back to G 1 phase. Cells were harvested at 2-h intervals. Half of cells were stained with propidium iodide, and analyzed by FACS. Another half of cells were prepared for cell ... Given the complexity of cell cycle regulation and the numerous intracellular compo- nents responding to oxidative stress, it is not surprising that Grx3-mediated cell cycle control may be depen- dent...

Ngày tải lên: 14/03/2014, 23:20

... (a) cells pulse treated (1 h) with cycloheximide early in the cell cycle (before RP) immediately suffer a long delay in the cell division cycle; (b) cells treated late in the divi- sion cycle ... as the emergent RP in the mammalian cell cycle) in terms of the multitude of reaction steps and interactions between the cell s molecular parts (e.g. cell cycle pro- teins) [18]. Numerous studies ... restriction point of the cell cycle. Cell Cycle 1, 103–110. 4 Yao G & Lee TJ, Mori S, Nevins JR & You L (2008) A bistable Rb-E2F switch underlies the restriction point. Nat Cell Biol 10, 476–482. 5...

Ngày tải lên: 15/03/2014, 09:20

... HeLa cells were incubated with nocodazole. Cells detached from the bottle by shaking were collected and named mitotic (M) cells. Residual cells were collected and named G 2 cells. These cells ... treatments; i.e. the DNA content of G 2 , M cells are almost double that of G 1 cells and the DNA content of S cells ranges between that of G 1 cells and G 2 or M cells (data not shown). BrdU was detected ... fluorescence and the reactivity to anti-BrdU sera, the cell- cycle stage was determined in each cell. Y. Komamura-Kohno et al. MCM4 phosphorylation in mammalian cells FEBS Journal 273 (2006) 1224–1239 ª 2006...

Ngày tải lên: 16/03/2014, 13:20

Ngày tải lên: 16/03/2014, 18:20

Ngày tải lên: 17/03/2014, 23:20

Ngày tải lên: 23/03/2014, 06:20

Ngày tải lên: 30/03/2014, 04:20