cell culture systems used for production of phytochemicals

... Scope of thesis .3  1.4 Organization of thesis 4  Chapter Overview of systems biotechnology 7  2.1 The advent of systems biology 7  2.2 Application of systems ... perspectives of systems biotechnology research Chapter Overview of systems biotechnology _ Chapter Overview of systems biotechnology 2.1 The advent of systems biology ... the utility of systems biology to gain a deeper understanding of cellular behavior through the development of rational analytic framework Through extensive use of computational methods for the modeling...

Ngày tải lên: 09/09/2015, 10:15

... functional status of cells is now routinely performed for plant cell cultures in order to permit accurate assessment of growth and metabolite production rates The availability of plant cells for quantitative ... (2) use of high producer plants for initiation of callus cultures; (3) biochemical analysis of derived cultures for their variability and productivity; (4) establishment of cell suspension cultures; ... patterns, bioinformatics is used here for this type of analysis of complex systems Bioinformatics refers to the extraction of biological information from genomic sequence and the reconciliation of multiple...

Ngày tải lên: 25/10/2013, 05:20

... pairs were taken from the data which was not used in the training and selected for the evaluation of the model We used the speech of four people for the evaluation The evaluation results are ... deletion of sentence ends We find some of phrases which come at the end of the sentence can be shortened or deleted If a sahen verb is used as the main verb, we can change it to its sahen noun For ... their news program for the purpose of our research References Figure Captions in the picture Figure Captions outside of the picture Most of the subjects preferred 2-line, outside of the picture captions...

Ngày tải lên: 08/03/2014, 06:20

... Table Plants used for gynecological disorders in Chhattisgarh Local Name Botanical Name Part Used Disease Method of Preparation Doses Local Name Botanical Name Part Used Disease Method of Preparation ... Botanical Name Part Used Disease Method of Preparation Doses Local Name Botanical Name Part Used Disease Method of Preparation Doses Local Name Botanical Name Part Used Disease Method of Preparation ... Name Part Used Disease Method of Preparation Doses 10 11 12 13 14 Local Name Botanical Name Part Used Disease Method of Preparation Doses Local Name Botanical Name Part Used Disease Method of Preparation...

Ngày tải lên: 28/03/2014, 14:20

... on the base of ultrasonic system with power kW of LLC «Ultrasonic technique - INLAB» production (fig 1) have developed sonochemistry reactor (USA patent №7,157,058 B2) for production of nano-particles ... sonochemically, for example, nano-particles of Fe, Co, Cu, Ag, Ni, Pd and so on The reactor is an effective unit for accelerating of chemical reactions, for example, the reduction of metal salts ... XXII Session of the Russian Acoustical Society Session of the Scientific Council of Russian Academy of Science on Acoustics Moscow, June 15-17, 2010 are added to the mixture to form the amorphous...

Ngày tải lên: 11/06/2014, 12:29

... temperature for oxidation to the required product In the case of pure b-PbO, the temperature is held at 6008C while for the production of red lead a range of 450 to 5008C is used In the case of red ... also used as the raw material feedstock for the production of pure PbO and red lead A schematic flow chart of the process used to Red lead is beneficial in improving the electrochemical performance ... Crystal structures of a-PbO ŽTetragonal and b-PbO ŽOrthorhombic for the Barton-pot samples had a range of 0.192 to 0.264 cm3 gy1 , and for the ball-mill samples, 0.20 to 0.298 cm3 gy1 For the median...

Ngày tải lên: 05/07/2014, 21:20

... 251.46 50.17 The DSC data for the PEILs were taken for appropriate molar ratios of the complexing agent (two for urea and one for oxalic acid) owing to the appearances of the PEILs that, overall, ... cyclic amide systems The physical and chemical properties of PEILs are congruent to those of the traditional ILs, but the range of structures for PEILs is likely to be different from that of ILs which ... practical uses for larger scale applications of other metals and bio-materials Although a multitude of recipes has been published to carry out the synthesis of ILs, not all laboratories focused on applied...

Ngày tải lên: 07/08/2014, 14:20

... was used as an indicator The degree of hydrolysis, X is calculated as below: X,% = (ml NaOH used) (molarity of NaOH) (average molecular weight of fatty acid) 10 (weight of sample) (1) The rate of ... Hydrolysis Profile Figure shows a typical hydrolysis profile of palm oil at temperature of 45ºC for an initial palm oil concentration of 0.1 g palm oil/ml iso-octane at agitation speed of 200 rpm ... hydrolysis reaction to achieve optimum production of fatty acids Therefore, the effect of pH buffer used in the hydrolysis medium was investigated in the pH range of 6.5 to 8.5 with other parameters...

Ngày tải lên: 07/08/2014, 14:20

... ANRr S61 a roF tcnitsid ylhgih saw nrettap noitcirtser eht hguoht ,devresnoc ylhgih ton era gnidnib remirp rof secneuqes tegrat eht taht seilpmi siht ;desu erew sremirp fo stes ruof ,]61[ tnemirepxe ... esoraga morf deifirup dna desicxe erew pb 715 erew taht stcudorp RCP nim rof Co27 ta pets noisnetxe lanif a htiw ,s 03 rof Co27 sretcarahc dlob ni nwohs era stnemgarf noitcirtser euqinU** sleg ... eht ,leg esoraga %3 gnisu nehw ,revewoH troffe dna emit erom seriuqer dna ,gnidaer dna gnildnah htiw seitluciffid yb detacilpmoc si PLFR-RCP ANRr S32 rof gniniats etartin revlis htiw sleg edimalyrcaylop...

Ngày tải lên: 07/08/2014, 18:21

... pattern of cell growth during the division cycle? The overwhelming majority of a cell' s mass is the cytoplasm; i.e., all that is not cell surface or cell Page of 15 (page number not for citation ... biosynthesis of the cell Thus, a cell that grows adding 0.1 unit of mass at each time interval would this continuously for one cell cycle At the instant of division the two daughter cells together ... growth of bacteria based on studies of cell size on synchronized cultures Conlon and Raff [20] have also proposed that the mass of eukaryotic cells increases linearly Page of 15 (page number not for...

Ngày tải lên: 13/08/2014, 23:20

... less than 10% was observed for 3D4/31 cells infected with the same virus concentration. PK15‐ C1  was  therefore  chosen  as  the  cell line  for development  of the  screening  platform.    A  large  batch of cells were frozen and used in the study until passage number 20 to ensure uniformity of ... (GraphPad  Software,  San  Diego  California  USA,  www.graphpad.com).  One‐way  Analysis  of Variance (ANOVA) with Bonferroni’s post‐test was performed for data obtained for Cell ELISA.  Cell Based Screening Assay for Inhibitors of Porcine Circovirus Type 2 (PCV2) Replication  ... assay for detecting PCV2 replication had to be developed. In line with these, the main goals of the study were:  ‐ Development  of a  primary  screening  method  for PCV2  replication  in  96‐well  and  384‐well plate format  ‐ Performing a POC trial for inhibition of viral replication using reference compounds ...

Ngày tải lên: 03/10/2015, 11:36

... case of Cu and Au, the addition of large amounts of CuO and AuO led to the formation of large pore diameter of catalysts in contrast with Ag loading [26] This could be attributed to the formation ... remarkable performance three times greater than that of Cu/SiO2 due to the role of ZnO/SiO2 in creating Cu þ and Cu0 as active species in driving the hydrogenation steps for the production of methanol ... in the synthesis of methanol from CO2 [12,13] The preparation of Cu/ZnO catalyst by physical mixture of Cu/SiO2 and ZnO/SiO2 resulted in formation of the ZnOx on the surface of Cu particles to...

Ngày tải lên: 08/10/2015, 23:14

... PCV2 replication. Screening assays in drug discovery are generally divided  into cell based and cell free  formats.  In  this  study,  a  whole cell approach  was  chosen  for development  primarily  because  of availability  of materials;  cell lines  and  ... 4. DISCUSSION    For uniformity of results, it was necessary to have an even  monolayer of cells per well and any  unnecessary  perturbation  that  might  lead  to  disruption  of the  cell sheet  ... afterwards  used to  infect  PK15‐C1  cells,  with  the  hope  of obtaining  higher infection rates1.    In  summary,  the  goal  of developing  a  cell based  method  for primary  screening  of ...

Ngày tải lên: 13/10/2015, 16:41

... various chemical compounds designated as substrates for cell maintenance, cell growth, and production Most bacteria used for the production of low- and high-molecular-weight organic compounds ... growing culture as ammonium hydroxide in response to the change of the pH An example of defined medium used for growing nonrecombinant E coli to high cell densities of 128 g liter1 dry cell mass ... containing carbon atoms can be used by these bacteria for the generation of biomass and product and for 10 Bacterial Cultivation for Protein Production the generation of energy through substrate-level...

Ngày tải lên: 24/04/2016, 10:44

... hydraulic jack for removal inner form Using hydraulic jack for movable of inner form to separate inner form and girder, then using gantry crane to lift core of inner form out of girder In Page ... by means of fabrication shall not be used Rebar arrangement of girder Page 15 Method Statement for Production of Super-T Girder Anchor hook D32 Steel pipe making hole for anchor bolt of upper ... Fabricating reinforcement assembly frame which is used to install reinforcement for super T girder • Reinforcement frame is fabricated with conforming dimensions to easily install reinforcement for super...

Ngày tải lên: 08/05/2016, 10:10

... Guidelines Procedures for the Production of UHT Chocolate Milk Beverages Introduction With the exception of milk powders several other dry ingredients are used in the production of chocolate milk ... number of decimal reductions of thermoresistant spores Therefore an acceptable level of defects should be established to rate the quality of the final product The maximum acceptable defect rate for ... Guidelines Procedures for the Production of UHT Chocolate Milk Beverages 3.4 Flowchart A typical flowchart for chocolate milk production Weigh the ingredients according to the formulation If the...

Ngày tải lên: 08/09/2016, 09:52

... the three Drosophila isoforms and two Leptinotarsa isoforms Such quantitative differences may prove important for in vivo functions In Manduca, the presence of a B-isoform increases transcriptional ... the natural isoforms of DmEcR are denoted DmEcRA, DmEcRB1, and DmEcRB2 The following protocols were used to construct the LdEcR cell culture vectors encoding its two natural isoforms (LdEcRA ... individual isoforms varied to some degree, as noted in previous work [9], the strength of signal did not correlate with differences in transcriptional activity (Fig 2C) In summary, each of the isoforms...

Ngày tải lên: 18/02/2014, 08:20

... of foot faults in the left and right forelimbs The sum of foot faults (SOFFF) in the forelimbs is defined as the number of faults of right and left forelimbs, whereas the differentiation of forelimb ... foot faults (DOFFF) is the difference between the number of faults for the right and left forelimbs SOFFF and DOFFF were used as an index of motor deficit CSF aspiration 80 μL of CSF were aspirated ... b-actin (forward: 5′-CTA CAA TGA GCT GCG TGT GG-3′, reverse:5′-CAG TCA GGA TCT TCA TGA GG-3′) The thermo-profile was 50°C for and 95°C for 10 min, followed by 40 cycles of 95°C for 15 s, 60°C for...

Ngày tải lên: 18/06/2014, 16:20

... with cell culture areas smaller than 0.2 mm2 The use of a micro-sized chip-based cell culture system in combination with reporter cells presents a powerful tool for the analysis of small cell ... permanent noninvasive characterization of individual cells in a cell layer The miniaturization of the cell culture area guarantees the observation of the complete cell culture area (Figure 1b, c) Currently, ... reduce the evaporation of the cell culture medium in the micro-sized cell culture chambers, a biocompatible cell culture chamber was positioned on the top of the MCC Each chamber of the covered silicone...

Ngày tải lên: 21/06/2014, 00:20