... amplified sequences into the HindIII andBglII sites, and the plasmid for production of VP3 was pre-pared by insertion of amplified sequences into BglII andKpnI cloning sites.Preparation of wt ... insertion of amplified sequences into theHindIII and BglII sites of pEGFP–N1. Amplified sequencesencoding tVP3 were inserted into the pEGFP–N1 plasmidusing BglII and SalI cloning sites. Amplified ... during infection, thecontribution of the minor proteins to cell destructionvia apoptosis is only moderate, suggesting that toxicity of the minor proteins is controlled during the infec-tious...
... wasidentified by collision induced dissociation of the four-fold protonated peptide ion. The charge state of thispeptide is unexpected: a peptide containing two basicamino acids (arginine and histidine) ... analyzed by ESI-QTOF MS(Fig. 3) in the positive ion mode, to get high qualityfragmentation pattern for peptide sequencing. Fifteen I. Catrein et al. Signal peptidase I activity in M. pneumoniaeFEBS ... peptide was identified as70-VGDTKLVALVR-80 containing a lysine in the middle of thesequence, which is probably modified by sulfonation (+136 Da).Signal peptidase I activity in M. pneumoniae I. ...
... would you do?15. If I got up earlier, I (not be) late for work.16. If I didn’t feel so tired, I (go) out with you. 17. If you (not pass) the exam, would you take it again?18. If I (be) you, I ... , I (not pass) the examination.5. It’s a beautiful house, and I (buy) it if I (have) the money, butI can’t afford it.6. I can’t imagine what I (do) with the money If I (win) the football ... English for Advanced Practice13. If she wins the prize, it will be because she ______ (write) very well. .14. Mike wished that the editors ____ (permit) him to copy some of their material....
... course. I ve forgotten a lot of it. I want to improve my writing skill. You know, sometimes I have to write letters in English.Kate: What about listening, Nga?Nga: It’s terrible. This is my biggest ... Noi. She leans English at school and at university but she has forgotten a lot. Her listening is terrible and her writing is not good. So she wants to improve her writing skill. She thinks ... her favorite English songs.c. She works for an international bank in Ha Noi.c. She works for a national bank in Ha Noi. e. Her listening is excellent. e. Her listening is terrible. True/...
... Coffin biết công việc của mình không ph i là làm một Thomas Edison thứ hai – mặc dù Coffin cũng là một nhà phát minh t i ba. Phát minh của ông là General Electric Co. Coffin theo d i hai ... chiếc xe t i của mình và gi i thiệu họ v i con chó Roy. Khi họ chen chúc nhau trên cabin chiếc xe bán t i của Walton, các nhà triệu phú ngư i Brazil bị h i liên tục. Cu i cùng thì họ cũng hiểu: ... nhiều nhất trên thế gi i. Edison không ph i là c i tên duy nhất che lấp tên ông. Th i đ i của Coffin (1892 – 1912) được biết đến như là “th i đ i Steinmetz” để tưởng nhớ kỹ sư i n t i năng...
... When a movie clip instance is assigned mouse events so that Flash recognizes it as a button, it retains allof its movie clip functionality. ã When a movie clip instance is assigned mouse ... Making the Mostof Attaching Mouse Events to Movie Clips Movie clip instances can also have attached mouse events (as shown in the preceding exercise). To make the mostof this powerful capability, ... employing properties and methods), buttons aren't independent timelines, while movie clips are—an important thing to remember when using the term this as a target path. For example, if you...
... meters with low cost 850 nm serial applications.10 Gb/s reliable transmission, design flexibility Laser-Optimized Fiber: Built for Price, Bandwidth, and Distance to Make the Mostof Your InvestmentPage ... Wide Wave Division Multiplexing schemeTable 1. Fiber Type and Distance per IEEE StandardsWith the same percentage of terminations as copper, fiber optic cabling and connectivity is a significant ... 300m using wavelength division multiplexing (WDM) electronics, which uses four laser sources at 2.5 Gigabit each and is cost prohibitive. With 62.5àm fiber making up much of the installed base,...
... sides on which points K, L, M and N lie to the intersection with thesides of the initial strip and with the line that divides it in halves. In this way we form twotrapezoids with the midlines ... sides A i A i+ 1and B i B i+ 1, respectively. ThenSOB i C i = p · OB i · OC i , SOB i D i = p · OB i · OD i and SOA i C i = p · OA i · OC i ,where p =12sin ∠A i OC i . Since OA i : OC i = ... OC i = OB i : OD i , it follows that S2OB i C i =SOB i D i SOA i C i . It remains to notice that the area of the part of the disk confined insideangle ∠A i OC i is greater than SOB i C i and...
... Consideration of reports submitted by States parties under article 18 of the Convention on the Elimination ofAll Forms of Discrimination against Women, Combined initial, second, third, fourth and fifth ... relating to marriage and family relations and in particular shall ensure, on a basis of equality of men and women: …[t]he same rights to 1 Convention on the Elimination ofAll Forms of Discrimination ... realization of these rights. We hope that the information presented is useful during the Committee’s review of Brazil’s compliance with the provisions contained in the Convention. If you...
... torecruitment of transcription factor II H (TFIIH) whichphosphorylates Pol II at the serines at position 5 (Ser5)within the heptapeptide repeats of the C-terminaldomain (CTD) of Pol II [147]. This ... an intrinsic ability to bind efficiently torecognition sequences within chromatin, and it is alsoevident that most consensus sequences within the gen-ome are not utilized. This is for a variety ... closely linked binding sites within adiscrete region. A corollary of this concept is thatbinding sites for transcription factors are not normallyActive chromatin and DNase I hypersensitive sites...
... generation,transmission and distribution of power within their jurisdiction. Chil e is oftenconsidered as the first Country to introduce liberalization in the electricity sectorin 1982. Regulatory reforms of ... variation of the optimal quantities of each player is smaller than 1 MW. For SFE_intercept model, the equilibriumoptimal strategies is attained when the variation of the optimal intercept of ... positive contribution in mitigating the strategic biddingbehaviors, pushing the uncompetitive electricity market performance towards to ahigh level competitive one [49].3.2 Market EquilibriumA...
... reactions involved in the reduction of theacceptor side of PSII [33]. In this simulated model, theJ I phase was calculated to be simultaneous with theinitial formation of PSII centers with ... acceptor side of PSII [3].The progressive reduction of the acceptor side of PSII leads to three distinct major phases of fluorescencerise from O to P with two intermediate steps, J (I 1) and I( I2) ... DCMU would inhibit completely the activity of PSII by preventing the reduction of the PQ pool[21,22], drastically channging the typical FI trace of thylakoids by eliminating the J–P rise [4,24]....
... enzyme–inhibitor com-plex. Mutation of this amino acid residue to a variabledegree affects the affinity of the enzyme–inhibitorinteraction. Inhibition activity remains upon mutation of TAXI -I His374 ... pastoristransformationThe contribution of amino acid His374 of TAXI -I to endo-xylanase inhibition was studied by mutation of this aminoacid residue into alanine, lysine or glutamine. Site-directedmutagenesis was ... observed differences in enzyme-inhibitor affinity.In this way, the exact mechanism of endoxylanase inhi-bition by TAXI -I and the specific contributions of theamino acid residues at the interface of...
... quantitation of microgram quantities of protein util-izing the principle of protein-dye binding. Anal Biochem72, 248–254.41 Harafuji H & Ogawa Y (1980) Re-examination of theapparent binding ... mechanism in which theTnI N-terminal region (from the structural TnC-bind-ing site to the inhibitory region) participates in theCa2+-dependent activation. In addition, at 15°C, sim-ilar activation ... substitution of Mg2+at site III or IV of vertebrate TnC with Ca2+causes the activation in vitro. Several observationshave indicated that the N-terminal region of vertebrateTnI is involved in...
... Sawazaki, K.,Iida, R. & Kishi, K. (1994) Detection of deoxyribonuclease I andII (DNases I and II) activities in reproductive organs of malerabbits. Int. J. Biochem. 26, 1025–1031.4. Takeshita, ... evolutionally critical change in thethermal stabilities of vertebrate DNases I. AmphibianDNasesIhaveaSer205insertioninaCa2+-binding site of mammalian and avian enzymes that reduces their thermalstabilities ... stabilities of DNase I in vitro might reflect the enzyme activities in vivo.Wefound that amphibian DNases I are characterized by a C-terminal end with a unique cysteine-rich stretch and byinsertion...