biomimesis of intelligent mobility manipulation and expression

... presence of IL-10, the induction of STAT1 and STAT3 phosphorylation by IL-10, and the expression of SOCS1 and SOCS3 mRNA in peripheral blood (PB) CD4+ T cells from RA patients Materials and methods ... crucial role in the activation of monocyte functions such as gene expression of the Fc gamma receptor type I and type III and of HLA-DR [31] More recently, high levels of activated STAT3, thought ... effect of IL-10 during the active phase Increased expression of cell surface IL-10R1 on RA CD4+ T cells The functional receptor complex of IL-10 consists of two subunits, the primary ligand-binding...

Ngày tải lên: 09/08/2014, 01:24

... growth and mortality of insect pests, Plutella xylostella (PX) and Spodoptera litura (SL).17th International Congress of Biochemistry and Molecular Biology Annual Meeting of the American Society of ... Sequence of recombinant plasmid DNA containing TI gene fragment Expression of recombinant MCoTI-II (ReMCoTI-II) The expression conditions such as ampicillin and IPTG concentrations, temperature and ... stop codon and Xho I restriction site was introduced on the end of the proposed synthetic trypsin inhibitor gene The sequences of the forward and reversed primers are shown on fig and of the synthetic...

Ngày tải lên: 12/02/2014, 10:20

... [19], and NMR spectroscopic data of p25a homologs from mice, Caenorhabditis elegans and humans [20–22] demonTable Determination of Vmax and Km for DAT, NET and SERT in the presence and absence of ... loss of survival Co -expression of DAT and p25a increased this cell loss, but co -expression with AS attenuated it Bars represents means ± SD of triplicates in one representative experiment of three ... membrane expression of DAT A potential effect of p25a on DAT function was addressed by expressing DAT alone and in the presence of p25a in HEK-293-MSR cells To validate the specificity of the C-20...

Ngày tải lên: 14/02/2014, 21:20

... neuron and postsynaptic muscle fiber contribute to the synaptic expression of PRiMA-linked G4 AChE and illustrate its temporal and spatial expression at NMJs Results Regulation of G4 AChE and PRiMA ... than that of AChE and AChR, extending into a peri-junctional zone where neither 3034 Fig Expression of PRiMA and G4 AChE in different muscles (A) Samples of extracts from adult rat soleus and tibialis ... activity of about 50% in the spinal cord and tibialis muscle (Fig 8B) Sucrose density gradient analyses showed a significant reduction of G1 and G4 forms in the spinal cord and of G1, G4 and A12...

Ngày tải lên: 18/02/2014, 08:20

... partial digestion of the DNA samples On the other hand, the absence of a second hybridization band in lane might be the result of insufficient sensitivity of our assay Rehybridization of the membrane ... this promoter region of pRIBOTEX and related vectors induces the transcription of genes cloned downstream of the promoter and their chromosomal integration [19] After 48 h of transfection, geneticin ... relevant and allowed us to detect the transient and stable periods of exogenous gene expression Genome organization of transformed parasites In order to explain the described changes of ADC mRNA...

Ngày tải lên: 19/02/2014, 07:20

... isolated and its genomic structure and expression analysis investigated This work will pave the way for further investigation of the biological function of this gene, and the probability of the ... 5¢-ACTACCTCATGAAGATCCTG-3¢ and reverse: 5¢-TTGCTGACCACATCTGCTG-3¢) served as a control for the quantity and quality of cDNA Semiquantitative analysis of RT–PCR products Suspensions of Carp head kidney and liver ... joining and the PAUP software (Fig 4) IFN-c was used as an outgroup, as this gene is structurally related to IL-10 Expression analysis of IL-10 by RT-PCR RT-PCR was used to analyse expression of the...

Ngày tải lên: 20/02/2014, 02:21

... switch and alters the expression of the two nuclear genome coded isoforms of CytOX V [10,32] The regulation of genes CytOX5a and CytOX5b, coding for the two isofoms Va and Vb, parallels that of genes ... different stages of adaptive response to hypoxia Based on the results of our study and those of Schumacker, it is reasonable to conclude that the effects of hypoxia on CytOX gene expression and its activity ... co-expressed under hypoxic (O2 < 0.5 lM) and heme deficient conditions [11] The coexpression of specific subunit V and cytochrome c isoforms indicates that these isoform pairs act synergistically to...

Ngày tải lên: 20/02/2014, 23:20

... other hand, it was inhibited by high concentrations of azide, an inhibitor of CD39 and most ecto-ATPDases [29–31], and high concentrations of fluoride, vanadate, and pyrophosphate, inhibitors of the ... absence and presence of the indicated concentrations of sodium azide.MgATP hydrolysis at pH 7.4 (s) and 6.4 (d); MgADP hydrolysis at pH 7.4 (h) and 6.4 (j) inhibition by azide was of the mixed and ... purification of the chicken liver ecto-ATPDase, its enzymatic properties and localization, cloning of the full-length cDNA and its expression EXPERIMENTAL PROCEDURES Materials The production and characterization...

Ngày tải lên: 22/02/2014, 04:20

... advantages and challenges of new information and communication technologies, including the Internet and mobile technologies, for the exercise of the right to freedom of opinion and expression, ... the enjoyment of the right to freedom of opinion and expression, because it leads to self-protective and over-broad private censorship, often without transparency and the due process of the law ... alia a violation of its obligation to respect the right to freedom of opinion and expression Although determining the origin of cyber-attacks and the identity of the perpetrator is often technically...

Ngày tải lên: 06/03/2014, 21:20

... the expression of this clone was highly induced in tissues of bacterial infected fish, a full-length cDNA clone as well as a genomic DNA clone were isolated and sequenced, and the expression and ... infection, and a strong product was present in samples prepared at 24 and 48 h after infection (Fig 4) Expression and modulation of trout IL-11 in cell lines The constitutive expression of IL-11 ... myofibroblasts, IL-b and TGF-b are potent inducers of IL-11 expression at both the mRNA and protein levels [8] Trout RTS-11 cells show constitutive expression of TGF-b mRNA, and IL-1b but not TGF-b...

Ngày tải lên: 07/03/2014, 16:20

... pCR2.1 vector, and sequenced on both strands The cloned cDNA was 1370 bp long and contained an ORF of 1257 bp, encoding a protein of 418 amino acids with a calculated molecular mass of 46 751 Da ... injections of a solution containing  50 lg of lipid-free protein preparation in 0.9% NaCl mixed with an equal volume of Freund's complete adjuvant Boost injections of 50-lg protein were performed 21 and ... dithiothreitol, 40 UámL)1 of RNase inhibitor, 2.5 mM dNTP, 0.2 mM oligo-dT 15±18mer, and 200 U of M-MLV reverse transcriptase First-strand synthesis was performed at 42 °C for 45 and then the enzyme...

Ngày tải lên: 08/03/2014, 16:20

... surface of the PO and many nerve fiber tracts and varicosities in trunks of the PO Cloning and sequencing of PO-CHH cDNA The first PCR of PO cDNA with a combination of a set of primers LF1 and LR1 ... Expression and release of CHH in the blue crab J S Chung and N Zmora 694 Results Identification and bioactivity of CHHs from ES sinus glands and PO Two RP-HPLC peaks (1 and 2) as presented ... Chung and N Zmora Expression and release of CHH in the blue crab Fig Sequence alignments of nucleotide and deduced amino acids of full-length PO-CHH (DQ667141) and ES-CHH (AY536012) PO-CPRP and...

Ngày tải lên: 16/03/2014, 06:20

... -III, -V and -VI genes and abolishment of transcription of the b4GalT-IV gene [32] Second, comparison of DNA microarray-based expression profiles between specimens of pancreatic cancer and normal ... of gene expression for proteins I and V and up-regulation of protein IV Of note, transcriptional regulation of b4GalTs-I ⁄ -V exhibits similarities noted in the Introduction In terms of signal ... groups of human lectins that can read and translate such differences are the C-type lectins, siglecs and galectins [42] The ensuing microarray monitoring of expression of 42 C-type lectins and siglecs-2,...

Ngày tải lên: 16/03/2014, 10:20

... members of the APP superfamily, such as the absence of the exon encoding the KPI domain and the lack of a second heparin-binding domain [3,4,13,26] Comparative analysis of the Xenopus and mammalian ... important domains within this APP superfamily member Expression pattern of Xenopus APLP2 mRNA The expression pattern of Xenopus APLP2 mRNA and the presence of alternatively spliced mRNA forms in various ... alternatively spliced exons Expression of APLP2 mRNA was detected in both neuronal and peripheral tissues of Xenopus (Fig 3) The presence of a 414 bp PCR product indicates the occurrence of the alternatively...

Ngày tải lên: 16/03/2014, 16:20

... IL-18A expression IL-18B expression IL-18 expression Gene expression Gene expression Gene organization Ó FEBS 2004 Fig Genomic DNA and the deduced protein sequence of rainbow trout IL-18 Exons and ... liver, muscle, skin, and spleen, although a lower expression level was observed for IL-18B Higher levels of expression were detected in gut, heart and kidney Modulation of IL-18 expression was studied ... poly(I:C), rIL-1b or a mixture of LPS and rIL-1b as described in the materials and methods In C, two of the three fish investigated are shown and the average ratio of the IL-18 expression relative to...

Ngày tải lên: 16/03/2014, 16:20

... prepared and subjected to PAGE in the presence of a low concentration of SDS and in the absence of b-mercaptoethanol After size separation (Fig 2; lane a), the proteins were transferred and – after ... the expression level of the glucanbinding protein at the beginning of the experiments is low However, after only day of incubation in the presence of 10 lgÆmL)1 curdlan, a strong upregulation of ... (Fig 6) The level of expression of the housekeeping gene, tubulin, was not altered by the presence of curdlan In view of the above finding, that curdlan causes phosphorylation of Tyr residue(s)...

Ngày tải lên: 16/03/2014, 16:20

... speculated that the physical role of MeJA is to mobilize JA [19] The proof of this hypothesis might come from a more detailed understanding of how plants form MeJA from JA, and vice versa A recent study ... cycles of 30 s at 94 °C, at 40 °C and at 72 °C, followed by 30 cycles of 30 s at 94 °C, at 45 °C and at 72 °C Gel electrophoresis, DNA elution and modification was carried out according to standard ... Bacterial expression and purification of tomato MJE To obtain unequivocal evidence of the identity of the cloned sequence, MJE cDNA was subcloned into a bacterial vector for heterologous expression...

Ngày tải lên: 16/03/2014, 18:20

... opinionated and polar fact into a single category, we obtain κ of 0.75 and a percentage agreement of 87% Thus, we conclude that opinion-relevant sentences, either in the form of an explicit expression of ... at the expression level We observe that the strength of the opinionexpression and the polar target polarity cannot be labeled as reliably as the polarity of the opinionexpression 61% of the agreed ... and Claire Cardie 2005 Annotating expressions of opinions and emotions in language Language Resources and Evaluation, 39:165–210 Theresa Wilson and Janyce Wiebe 2005 Annotating attributions and...

Ngày tải lên: 16/03/2014, 23:20

... which contained 20 ng of first strand cDNA, · PCR buffer (20 mM Tris/HCl pH 8.4, 50 mM KCl), 0.2 lM of each primer, 1.5 mM MgCl2 and 0.2 mM of dNTPs PCR Construction and screening of a grass carp genomic ... KCl), lM of each primer, 0.2 mM of dNTPs, 1.5 mM MgCl2 and U of Taq DNA polymerase (Invitrogen) The PCR program consisted of predenaturation at 94 °C for min, followed by 35 cycles of amplification ... performed on first-strand cDNAs that were reverse transcribed from total RNA by use of Superscript II reverse transcriptase (Invitrogen) and consisted of 20 ng of first strand cDNA, · PCR buffer...

Ngày tải lên: 17/03/2014, 03:20