bacteria bacteriophages and plasmids

... (XMG1.2, C17.8, SXC-1, B11-3 and TRFK4 clones, respectively; PharMingen) Detection limits were 40 pg/mL (for IFN-γ and 10 pg/mL (for IL-12 and IL-10, TNF-α, IL-4 and IL-5) Elispot Assay The ELISPOT ... later, the number of live bacteria in the lungs was determined and expressed as CFU/lung (B, C) Sixty days after the last immunization, the mice were challenged and 30 (B) and 70 (C) days later, ... BCGsc, DNA-HSP65 and BCGsc/DNA (D) ᭜ BCGin, DNA-HSP65 and BCGin/DNA vs Infected * BCGin/DNA vs BCGsc, DNA-HSP65 and BCGsc/DNA # BCGin vs BCGsc (E) ᭜ BCGsc, DNA-HSP65, BCGin/DNA and BCGsc/DNA vs...

Ngày tải lên: 14/08/2014, 19:22

... (Boehringer-Mannheim) treatment [26] and phenol/chloroform extraction PCR using pig genomic DNA (100 ng) and primers p1 and p2 (Table 1) hybridizing to 5¢ untranslated exons and 3, respectively, was carried ... insert of C.1 and in the pGL3-Enhancer plasmid, NdeI (Dnt1–164) and PvuII (Dnt1–475) fragments were also deleted, yielding constructs C.2 and C.3, respectively The presence of the insert and its orientation ... in the plasmids was checked using restriction enzyme mapping and sequencing For the position of the relevant restriction sites see below Transient transfections and luciferase assays COS7 and PEC-A...

Ngày tải lên: 08/03/2014, 16:20

... aggrecan and type II collagen genes and upregulates mRNA expression of matrix metalloproteinase (MMP)-1, -3 and -13 and ADAM-TS4 (a disintegrin and metalloproteinase with thrombospondin motif 4) and ... regulation and degradation in normal and degenerate NP cells isolated from human adult tissue and investigates how TNF-α and IL-1β may be involved in regulation of themselves and each other and their ... Histological studies by Bachmeier and colleagues [10,11] have shown TNF-α and its receptors to be expressed in normal IVD and upregulated with age and degeneration Seguin and colleagues [12] have demonstrated...

Ngày tải lên: 09/08/2014, 14:20

... polymicrobial sepsis and dehydroepiandrosterone treatment in a rodent model Shock 2003, 20:338-346 McGowan JE Jr, Barnes MW, Finland M: Bacteremia at Boston City Hospital: Occurrence and mortality during ... fluid regulation during and after soft tissue trauma and hemorrhagic shock in males and proestrus females Shock 2003, 20:144-148 Diodato MD, Knoferl MW, Schwacha MG, Bland KI, Chaudry IH: Gender ... WG: Metabolism and actions of dehydroepiandrosterone in humans J Steroid Biochem Mol Biol 1991, 40:599-605 Angele MK, Catania RA, Ayala A, Cioffi WG, Bland KI, Chaudry IH: Dehydroepiandrosterone:...

Ngày tải lên: 13/08/2014, 18:22

... kDa band in T cells A Cb-specific antibody anti-Cb(SNO157) (vi) recognized the 47 kDa band in T-cells, and the 40- and 47 kDa protein bands in Jurkat cells (C) Linear depiction of Ca1, Cb1 and ... variants and not cross-react with Ca (Fig 1C and Experimental procedures) AntiCb2(SNO103) recognized the 47 kDa protein band in T cells and Jurkat cells, while anti-Cb(SNO157) recognized a 47 kDa band ... anti-PKAccat (ii) and antiPKAbcat (iii), and a commercial monoclonal antibody, anti-Cmono (iv) These antibodies all recognized several different protein bands, but two bands of 40 and 47 kDa were...

Ngày tải lên: 16/03/2014, 18:20

... concentrations of ADP (1 mm and 10 mm) The observed changes in Rh with ADP were significantly lower: at mm and 10 mm ADP the Rh increased to and stabilized at % 30 nm and % 150 nm, respectively ... Annealing between CLS and GTS strands forms a 16-mer duplex with G.T mismatch at the ninth base from the 5¢ end of CLS and the corresponding homoduplex is formed by annealing CLS and GCS Protein purification ... fluorescence and other biochemical assays The principal players in the system namely, MutS and MutL proteins showed a reasonably narrow distribution of Rh values with a peak at 10 nm and nm, 6229...

Ngày tải lên: 07/03/2014, 12:20

... complex C1 disappeared and a new complex, SC1, with a higher molecular mass was formed (lane 11), and, when anti-Sp3 IgG was used, bands C2 and C3 disappeared and bands SC3a and SC3b appeared (lane ... promoter, are essential for both basal and PMA-mediated induction of DNase II transcription These sites bind Sp1 and Sp3, and protein levels and binding of Sp1 and Sp3 are increased in PMAtreated ... lg of phRL-TK DNA (Promega), and 50 lgÆmL)1 of DEAE-dextran (Sigma), and incubated at room temperature for 15 The cells were centrifuged and the pellet was washed, and resuspended in RPMI 1640...

Ngày tải lên: 23/03/2014, 17:21

... to naïve rats and blood samples were drawn 2, 6, 24 and 48 hours after dipyridamole administration and subjected to Ficoll hypaque gradient The PBMCs were then washed with PBS and protein extracts ... performed and the haematoxylin was then removed by quick dip in acid alcohol and the slides were washed in ammonia water, dehydrated and mounted with Permount Human blood sample collection and separation ... extracted using standard techniques, and by sequencing the RT-PCR product and comparing the sequences with the known sequences (ADORA3-L77729, L77730) The optical density of the bands (Et-Br) was...

Ngày tải lên: 09/08/2014, 08:23

... analyses and genotyping, and helped with data interpretation BRM participated in study design, and helped with data collection and analysis GVR conceptualized and designed the study, and revised ... WTs and R6/2s indicate saline treatment, and WTc and R6/2c indicate ceftriaxone treatment N refers to number of animals per group Genotype and CAG repeat length We used PCR for genotyping and ... cortex and striatum at 13 weeks of age Immunoblots (A, C) and quantitative analysis (B, D) of the percentage ratio of GLT1/b-tubulin in cerebral cortex and striatum, respectively (**P < 0.01 and...

Ngày tải lên: 10/08/2014, 05:21

... conceptualized and designed the study and participated in writing and revision process Kwang Ho Ko contributed study design and revised the manuscript for intellectual content All authors read and approved ... Activation of PI3K and Akt pathway and its role in FMRP production and cell viability ETO-treated HeLa cells were analyzed using Western blot to investigate the activation of PI3K (A) and Akt (B) Western ... Growth factors and integrins activate Akt by phosphorylation at Ser473 and Thr308, which leads to the modulation of Bad, caspase 9, and transcription factors including forkhead family and NFkB [50]...

Ngày tải lên: 10/08/2014, 05:21

... cell and BGC/shSTC cell) were confirmed in both mRNA and protein (Additional file 1, Figure S1B, Figure S1A) level All these cells were cultured under standard culture conditions for 24 h, and ... determined by ELISA (F) CGP53353 and PD98059 could inhibit PKC bIIand ERK activation, respectively Cells and cell culture Human gastric adenocarcinoma cell line BGC823 and Human umbilical vein endothelial ... effective siRNA fragment was converted to shRNA and then was inserted into pSiencer4.1 PcDNA3.1/STC-1 and pSilencer4.1/ STC-1-shRNA plasmids were constructed and transfected into BGC823 cells with Lipofectamine...

Ngày tải lên: 10/08/2014, 05:21

... ≥median + standard deviation and low GAD67 or low MUC5AC gene expression as ≤median - standard deviation Based on this criteria, healthy smokers with high GAD67 and high MUC5AC gene expression and healthy ... subsequently collected separately in ml of LHC8 medium (GIBO, Grand Island, NY) An aliquot of this was used for cytology and differential cell count and the remainder was processed immediately for RNA extraction ... chondrocytes and connective tissue near smooth muscle [21] GAD65/67 mRNA has been detected in human and mouse airway epithelium at the mRNA level by RTPCR and at the protein level by Western analysis and...

Ngày tải lên: 12/08/2014, 11:22

... unfailing support during my candidature He provided me the opportunity to learn and pursue this project under his guidance and his advice, patience and kind understanding has encouraged me to ... in HCC and the HCC patients’ prognosis and other clinicopathological characteristics; and Determine the effect of C/EBPα and C/EBPα knocked-down cells on HCC tumor growth using in vitro and in ... Index and Index ≤ : p-value 0.016* Comparing Index and Index ≤ : p-value 0.009* Comparing Index and Index = : p-value 0.169 Comparing Index and Index = : p-value 0.088 Comparing Index and Index...

Ngày tải lên: 02/10/2015, 17:14

... recombinase-mediated recombination [27] of two plasmids, one containing the right hand portion of the adenoviral vector genome and a lox site, and the other plasmid, pAvhUCP3lx, containing the ... preferentially and abundantly in skeletal muscle and brown adipose tissue Biochem Biophys Res Comm 235, 79–82 Rolfe, D.F & Brown, G.C (1997) Cellular energy utilization and molecular origin of standard ... the left portion of the viral genome, the UCP3 expression cassette, and a lox site as described previously [26] Both plasmids and the Creencoding plasmid were cotransfected into AE1-2a cells (A549...

Ngày tải lên: 21/02/2014, 15:20

... either the bacterial agent LPS or the mite allergen, Der p MATERIALS AND METHODS Materials and animals Deuterated anandamide, PalEtn and 2-AG were synthesized from [2H4]palmitic acid and [2H8]arachidonic ... macrophages and lymphocytes are able to produce a higher amount of anandamide and/ or 2-AG [21,23–26] IgE-dependent stimulation of RBL-2H3 cells also leads to the formation of anandamide and of its ... were obtained: a top leukocyte band containing mononuclear cells (monocytes and lymphocytes) and a lower band containing polymorphonuclear leukocytes (granulocytes) and the red cells The PBMC were...

Ngày tải lên: 22/02/2014, 07:20

... [10] These structures have been implicated with channel function (e.g in ligand-gated and voltage-gated K+ channels) [16], and thus could provide an explanation for the observed channel activity ... depicted in yellow (Tic110, Tic40 and Hsp93), redox-regulatory subunits in blue (Tic62 with associated FNR, Tic55 and Tic32), the proposed alternative import channel Tic20 and the intermembrane space ... function [24] Chaperones subsequently have been the main candidates for this ATPase activity and, indeed, members of the Hsp60 [chaperonin (Cpn)60] and Hsp100 (Hsp93) families have been found to interact...

Ngày tải lên: 07/03/2014, 03:20

... Purification and activation of the precursor and enzymic properties Eur J Biochem 194, 721–730 20 Das S, Mandal M, Mandal A, Chakraborti T & Chakraborti S (2004) Identification, purification and characterization ... binds MMP-3 and dentin matrix protein-1 binds MMP-9, all with a : stoichiometric ratio and binding constants in the nanomolar range [26] These SIBLINGs and MMPs are also co-expressed and colocalized ... interaction between MMP-2 ⁄ PEX and avb3 integrin [54] Activation through interactions with elastin, heparin and CD151 ProMMP-2 and active MMP-2 binds to soluble and insoluble elastin through the...

Ngày tải lên: 15/03/2014, 00:20

... cAMP and Ca2+ ions – thus act to activate eEF2 kinase and switch off elongation (Fig 3) We will return to the issue of energy demand and the control of elongation below REGULATION OF eEF2 AND ... and ventricular myocytes [53] This is associated with a decrease in the activity of eEF2 5364 G J Browne and C G Proud (Eur J Biochem 269) Fig Inhibition of eEF2 and elongation by energy demand ... (eEF1A and eEF1Babc) are phosphoproteins and are targets for a number of protein kinases These include casein kinase (CK2), a constitutively active protein kinase, which phosphorylates the a and...

Ngày tải lên: 23/03/2014, 21:20

... import and export of human germ cells and fertilized ova, including their biological components and derivative products, is free from and to all EU member-states The import and export to and from ... was also requested on the activities and volume of reproductive cell interchange, including past and present imports and exports to and from third countries, and on any suspect or unauthorised ... time received imports of sperm donations: Germany - from Denmark, the Netherlands and the US; Finland - from Denmark and the US; Spain - from the US No figures were provided Sperm Cells Member...

Ngày tải lên: 28/03/2014, 16:20

... Can the propeptide and the CT-peptide behave synergistically and they share an identical fate? As mentioned previously, there exist instances whereby peptides located at the N- and the C-termini ... Enzymatically active mPC1 ⁄ was incubated at pH 6.0 with either propeptide and CT-peptide alone or with mixture of propeptide and CT-peptide and the released AMC was measured The propeptide was obtained as ... mature secretory granules, colocalizes with ACTH and b-endorphin and is released in the medium upon secretagogue-mediated secretion (N Rabah and C Lazure, unpublished data) Interestingly, although...

Ngày tải lên: 30/03/2014, 08:20