bacteria macromolecules and vesicles

... prominent bands employing the Weber and Osborn protocol In this study on D magna Hb subunits, three bands were found using the Laemmli protocol and only a single band with the Weber and Osborn ... mass and sedimentation coefficient of Hb macromolecules Crustacean hemoglobins are large polymers with molecular masses between 240 and 800 kDa, and sedimentation coefficients between 11 and 19 ... were 11 for DHb and DHb2 and 12 for DHb3 and DHb4, respectively For identification of the carbohydrates bound to D magna Hb subunits, saccharides were released by methanolysis and separated by...

Ngày tải lên: 16/03/2014, 13:20

... efforts and ligands tested with other riboswitch candidates are presented in Additional File SAM/SAH-binding RNA The coenzyme SAM and its reaction by-product SAH are frequently targeted ligands ... Collinsella-1 y n n Actinobacteria, human gut RF01700 crcB Y Y Y Widespread, bacteria and archaea RF01734 Cyano-1 y n n Cyanobacteria, marine RF01701 Cyano-2 Y n n Cyanobacteria, marine RF01702 ... that is common to all bacteria containing epsC RNAs Signalling molecules also regulate EPS synthesis in some bacteria [36,38], and are therefore also candidate riboswitch ligands The epsC motif...

Ngày tải lên: 09/08/2014, 20:21

... et al (1999) and Marques et al (1999) investigated the association of JR400 and SDS/DDAB vesicles, and that of LM200 and SDS/DDAB vesicles In the solution phase, faceted vesicles and disklike ... cationic JR400 and PVP PVP tends to be positively charged in aqueous solution The vesicles are negatively charged catanionic SDS/DDAB vesicles, SDS/alprenolol vesicles, and SDBS / CTAB vesicles There ... cholesterol and vesicles composed of DODAC or biological cells Lee et al (2005) examined association between hm-chitosan and SDBS/CTAT vesicles The association between hmPEG and SDS/DDAB cationic vesicles...

Ngày tải lên: 16/10/2015, 12:00

... estuarine sediments (St D and E; GM = 4.7 x 103 and 4.9 x 103 CFU/100 g dry weight, respectively) and then marine sediments (St A, B and C; GM = 7.3 x 102, 4.6 x 102 and 4.5 x 102 CFU/100 g dry ... E.W., Burke, J., and Spain, A (2003) Fecal indicator bacteria are abundant in wet sand at freshwater beaches Water Res., Vol.37, No.16, 3978-3982 Anderson, K.L., Whitlock, J.E., and Harwood, V.J ... Persistence and differential survival of fecal indicator bacteria in subtropical waters and sediments Appl Environ Microbiol., Vol.71, No.6, 3041-3048 Bissett, A., Bowman, J., and Burke, C (2006) Bacterial...

Ngày tải lên: 05/09/2013, 10:15

... Hoagland solution with 10 mg/L phenol and 30 fronds of sterile S polyrrhiza DNA extraction from the S polyrrhiza bacteria association and suspended bacteria in bulk water S polyrrhiza-associated bacteria ... gene and C23O gene in S polyrrhiza-associated bacterial communities in test systems A and B and those in the suspended bacterial community in test system C are shown in Fig In test systems A and ... Fig - PCR-DGGE banding patterns of 16S rRNA gene, C12O gene and C23O gene of Spirodela polyrrhiza-associated bacterial communities in test systems A and B and that of suspended bacterial communities...

Ngày tải lên: 05/09/2013, 10:15

... River Rain Water Bacterial Density (CUF/mL) 10000 Bacterial Density (CFU/mL) Bacterial Density (CFU/mL) 100000 Bacterial Density (CFU/mL) Deionized Water 100000 Journal of Water and Environment ... silicon carbide porous plugs and incubated in the dark with shaking at 20°C for d The tetracycline concentration and density of bacteria were determined before, after, and during the incubation ... of bacterial density of tetracycline-degrading heterotrophic bacteria was calculated from the results using a computer program (Kohno and Suzuki, 1999) The density of tetracycline resistant bacteria...

Ngày tải lên: 05/09/2013, 10:15

... document to minimize microbial food safety hazards in fresh and minimally processed fruits and vegetables and mandated a Hazard Analysis and Critical Control Point (HACCP) program to achieve a 5-log ... harvesting, postharvest handling, washing, and cooling and storage PREVENTIVE MEASURES IN THE ORCHARD Contamination of fruits with feces of animals such as deer,8 seagulls,9 and cattle and other ruminants10 ... The design and implementation of HACCP and application of alternative processes provide the current strategy for microbial control and ensuring shelf life and safety of fruit juice and beverages...

Ngày tải lên: 25/10/2013, 21:20

... Effect of pH on MTX and cGMP transport into membrane vesicles from humans and from WT and KO mice (A) Effect of pH on MTX transport Erythrocyte membrane vesicles from humans and WT and KO mice were ... with murine erythrocyte vesicles On the one hand, PGE1 and PGE2 reduced cGMP transport to only 57% and 59% of the control value, and the inhibitory effect of dipyridamole and indomethacin was also ... erythrocyte vesicles from WT and KO mice (A) Erythrocyte membrane vesicles from WT and KO mice were incubated for 30 at 37 °C with 1.8 lM [3H]cGMP ATP-dependent transport of cGMP into vesicles from...

Ngày tải lên: 07/03/2014, 11:20

... Training for Field Epidemiologists Investigation strategies and methods Developed by the Department of Epidemic and Pandemic Alert and Response of the World Health Organization with assistance ... At the end of the presentation, participants should: • Understand the principle of cultivating viruses • Understand the methods and problems with cultivating viruses Laboratory Training for Field ... Laboratory Training for Field Epidemiologists Culture procedure Use of a variety of cell sources and techniques Treatment of the specimen prior to inoculation Follow-up Viral detection: • Non specific...

Ngày tải lên: 15/03/2014, 13:03

... in liver and spleen Fruit bats not show any symptoms Best candidate to be the reservoir More research needs to be done Geography  The link between human infection by the Ebola virus and their ... gorilla and chimpanzee populations - The outbreaks coincided with the outbreaks in wild animals - The same distinct viral strains were isolated in animal carcasses and in the bodies of those who handled ... photophobia and hyperlacrimation) - hearing loss - unilateral orchitis( inflammation of one or both of the testes) ** These conditions are usually relieved with the treatment of 1% atropine and steroids...

Ngày tải lên: 15/03/2014, 13:09

... wetland and the pond, with out¯ow TTC and heterotrophic bacterial concentrations for the wetland, and with in¯ow and out¯ow concentrations of heterotrophic bacteria for the pond Physical and ... those of the wetland water column samples The water column and sediment bacterial concentrations for the wetland and pond are given, respectively, in Figs and The concentrations of bacteria in sediments ... ef®ciencies for the wetland were 79, 85 and 87% for TTC, ENT and heterotrophic bacteria, respectively However, the difference between in¯ow and out¯ow concentrations of bacteria was generally...

Ngày tải lên: 15/03/2014, 23:20

... relationship between cyanobacteria and heliobacteria determined by phylogenetic analysis of photosynthetic genes [25] No protein bands were immunodetected in cell extracts from E coli K12 and DH5a or nonphotosynthetic ... subfamily and a possible counterpart from a bacterial ancestor Experimental procedures Organisms and growth conditions The photosynthetic bacteria used in this study are summarized in Table Cyanobacteria ... this protein assembly: the ‘eukaryotic’ (fungi, plants, metazoa, and most protists), and the ‘prokaryotic’ (bacteria, archaea, and photosynthetic eukaryotes) [12] Family I is therefore an ancient...

Ngày tải lên: 16/03/2014, 11:20

... Phylogeny and in situ Localization of Bacterial Isolates and Communities 23 Molecular Phylogeny and Ecology of Azospirillum and Other Nitrogen-fixing -Subclass Proteobacteria ... processes, and relevant chemical models; genetics and regulation; genomes and genomics; associative, endophytic, and cyanobacterial systems; actinorhizal associations; leguminous symbioses; and agriculture, ... the proliferation of soil bacteria in the rhizosphere, and how the first nitrogen-fixing bacteria, including cyanobacteria were isolated The chapter covers the landmarks and scientific concepts...

Ngày tải lên: 16/03/2014, 11:45

... transport and accumulation of tc across the Gram-negative cell envelope has been presented by Nikaido and coworkers ([40,41] and references cited therein) In the medium, as well as in the periplasm and ... based on powerful selection and screening systems in E coli [104,105] and in S cerevisiae [88] have lead to a profound understanding of how DNA binding, inducer binding and dimerization function ... screening and selection systems in both pro- and eukaryotes, the Tet system is becoming more and more capable of modeling the sophisticated regulatory setups needed [48,51] to analyze complex and multifactor...

Ngày tải lên: 23/03/2014, 21:20

... Lineweaver–Burk plots (see Fig 1) The Km and Vmax values are equal to 5.1 lM and 1.2 lmolÆmin)1Æmg protein)1 and 19.6 lM and 0.1 lmolÆmin)1Æmg protein)1 for NADH-oxidase and NAD+-reductase reactions, respectively ... Amsterdam, the Netherlands) Bacteria were grown anaerobically with succinate as the substrate and nitrate as the added terminal electron acceptor under growth conditions described by John and Watley [21] ... direct and reverse reactions carried out by the membrane-bound P denitrificans complex I are estimated and compared with those of the mitochondrial enzyme The data on inhibition of NADH-oxidase and...

Ngày tải lên: 23/03/2014, 21:21

... biosynthesis in bacteria Attempts have been made to express metallothioneins and polyphosphates in bacteria However, bacterial expression of metallothionein (MT) was shown to be unstable [8] and had ... kinase (ppk) gene in transgenic bacteria to increase the levels of polyphosphates and mercury resistance [21,22] Transgenic bacteria expressing ppk was shown to withstand and accumulate up to 16 μM ... 2A and 2C) Mercury bioassays performed with the pBSK-P16Sg10-ppk-rpsT bacteria revealed that this transgenic bacteria was able to grow in Hg concentrations of up to 40 and 80 μM after 16 and...

Ngày tải lên: 23/03/2014, 22:20

... PamOlePtdCho vesicles (solid curve) and PamOlePtdCho/PDA vesicles (broken curve); and (B) vesicles containing both negative and zwitterionic phospholipids – small unilamellar Ole2PtdSer/PamOlePtdCho vesicles ... lipid–poly(diacetylene) (PDA) vesicles and vesicles not containing PDA (vesicles comprising phospholipids only) Correlation functions [C(t)] of Patman are shown in: (A) vesicles containing zwitterionic ... PDA vesicles and Ole2PtdSer/PamOlePtdCho/PDA vesicles, respectively The percentage colorimetric response (%CR, see the Materials and Methods) induced by the peptides in (A) PamOlePtdCho/PDA vesicles...

Ngày tải lên: 30/03/2014, 20:20

... the rib genes in bacteria classified in the Proteobacteria c-subdivision and Gram-positive bacteria In Enterobacteriaceae ribX, ribD and ribE are organized in a cluster but ribC and ribBA are not ... Growth (h) and bioluminescence (s) curves of the parent strain in 3% and 1% NaCl are shown in panel B1 and B2, respectively, and those of M1, M2 and M3 in 3% NaCl are shown in B3, B4 and B5, respectively ... light and total amount of flavins produced in the cells Five growth curves of the parent strain cultured in 3% and 1% NaCl, and M1, M2 and M3 in 3% NaCl are shown in Fig 2A Growth rates and maximum...

Ngày tải lên: 31/03/2014, 08:20

... Hancock, W J Henzel, and C Horvath, in "'HPLC of Biological Macromolecules: Methods and Applications" (K M Gooding and F E Regnicr, eds.), p 145 Marcel Dekker, New York, 1990 ~eW R Melander, H.-J Lin, ... polypeptides and proteins, while cases (a) and (b) demonstrate a typical dependency of retention on of polar peptides and small polar proteins The S and log k0 values for polypeptides and proteins ... pre- 71,W R Melander, D Corradini and C Horvath, ,I Chromatogn 317, 67 (1984) 77 (7 Horvath, W Melander, and I Molnar, J Chromatogr 125, 129 (1976) 7'~J F Pollit, G Thdvenon, L Janis, and F E Regnier,...

Ngày tải lên: 11/04/2014, 09:46

... cyanogen bromide fragments of Mb and the live synthetic peptide standards Peptide standards 1, 2.3, 4, and contain 10, 20, 30, 40, and 50 residues, respectively, and their sequences are shown in ... Rabel, J F Stobaugh, T D Williams, and D G Vander Veldc, Anal Chem.68, 67R (1993) i~, K M Gooding and F E Regnier (eds.), "HPLC of Biological Macromolecules: Methods and Applications." Marcel Dekker, ... Battersby, and W S Hancock, in "HPLC of Peptides and Proteins: Separation, Analysis and Conformation" (C T Mant and R S Hodgcs, eds.), p 825 CRC Press, Boca Raton, FL, 1991 57C T Wehr and R E Majors,...

Ngày tải lên: 11/04/2014, 09:46