... nâng cao hiệu quả công tác quản lý nghiên cứu khoahọc lĩnh vực y - dược
học tại HVQY.
Keywords: Nghiên cứu khoa học; Y học; Dược học; Quản lý khoahọc
Content
1. Lý do chọn đề tài nghiên cứu ... Quân y trong
lĩnh vực Y - Dược học?
7. Giả thuyết nghiên cứu
Để nâng cao hiệu quả công tác quản lý NCKH trong lĩnh vực Y - Dượchọc tại
Học viện Quân y, nghiên cứu kiểm chứng các giả thuyết ... ngành y tế nói chung và ngành Quân y nói riêng thì các đề tài nghiên
cứu được tiến hành ở các lĩnh vực như yhọc cơ sở, yhọc quân sự, yhọc lâm sàng, dượcbào
chế, nghiên cứu y sinh học Việc...
... rapidly passed back
from the R1E subunit to the tyrosyl of the R2F sub-
unit. Subsequently, the dicysteine unit is re-reduced by
an exogenous reductant and catalytic activity is
restored. By not ... catalysis, as expected.
The tyrosyl radical of the R2F subunit was also
monitored during the course of the enzyme assay.
Curiously, under conditions where the product was
formed, the tyrosyl radical, ... 408 nm, characteris-
tic of a tyrosyl radical (Y
ặ), which is sensitive to the radical scavenger
hydroxyurea. A novel enzyme assay revealed the direct involvement of Y
ặ
in ribonucleotide reduction...
... activity. NPY is able to act through
five different receptors (Y1 , Y2 , Y4 , Y5 and y6 ) that
vary in their binding profiles and in their distribution
in the central nervous system and periphery. Y1 , Y2
and ... Cytokines, estrogen, and postmeno-
pausal osteoporosis – the second decade. Endocrinology
139, 2659–2661.
Leptin
NPY
Y2
Y1
Y2
NPY
NPY
Osteoblasts
HypothalamusFat tissue
Sympathic
nervous
system
NPY
Circulating ... response is
mediated centrally, the Y1 response is mediated by
osteoblastic Y1 . Thus, hypothalamic signals sustain a
systemic regulatory influence via Y2 , whereas osteoblas-
tic Y1 enables additional...
... carboxypeptidase Y; FM4-64, N-(3-triethylammoniumpropyl)-4-(p-diethylaminophenylhexatrienyl) pyridinium dibromide; GFP, green
fluorescent protein; I
C
, carboxypeptidase Y inhibitor; PC, phosphatidylcholine; ... Hayashida M, Fujii T, Hata Y, Hayashi R &
Ueda M (2004) Crystallization and preliminary X-ray
analysis of carboxypeptidase Y inhibitor I
C
complexed
with the cognate proteinase. Acta Crystallogr ... carboxy-
peptidase Y in yeast. Biochem Biophys Res Commun 60,
1051–1057.
3 Lenney JF, Matile Ph, Wiemken A, Schellenberg M &
Meyer J (1974) Activities and cellular localization of
yeast proteases...
... ovary; cNPY, chicken neuropeptide Y; cPP, chicken pancreatic polypeptide; cPYY, chicken peptide YY; Hsa,
Homo sapiens chromosome; pNPY, porcine neuropeptide Y; PP, pancreatic polypeptide; pPYY, ... > cPP (see Table 1). Interestingly, pNPY had a
lower affinity than pPYY, thereby making it unlikely
that cNPY would bind with higher affinity (they differ
by only two conservative replacements as ... determine the
affinity of cPYY also to the previously cloned Y- family
receptors in chicken. Among the intact peptide ligands,
the rank order of potency was pPYY > pNPY >
cPYY > cPP (see Table...
... [24–27]. There are two types of
nongerm cell CSD proteins and these are called dbpB
(also known as YB-1, MSY-1, chkYB-1b, EF1A, p50 and
FRGY1) and dbpA (MSY4, chkYB-2 and YB2/RYBa).
DbpB and dbpA ... [47],
dbpB/YB-1 binding to the IL-2 5Â-UTR [31] and the VEGF 5Â-UTR
CSD site 1. MSY1, ChkYB-1b and FRGY1 are dbpB/YB-1 proteins.
MSY-4 and chkYB-2 are dbpA proteins, and MSY2 and FRGY2 are
germ ... of stably transfected cell lines was as
described previously [15]. Hypoxic conditions (1% O
2
)were
generated in a hypoxic chamber (Edwards Instrument
Company, Sydney, Australia).
Analysis of...
... th y sản”,“Công nghệ chế
biến tôm chua.”, “Công nghệ chế biến mắm ruc, mắm nêm”,
3. Ths. ,“CÔNG NGHỆ CHẾ BIẾN NƯỚC MẮM”, “Công
nghệ chế biến th y sản”, ... CHẾ BIẾN NƯỚC MẮM”, “Công
nghệ chế biến th y sản”,
4. “X y dựng quy trình sản xut chế phẩm vi khuẩn lactic ứng dng
trong lên men tôm chua”, ...
4.4.2 Mm ruc
Bng 4.6 ng và s c khi ch bin mm ruc
Nguyên nhân
+
+
không...
... commonly observed in adenylate-
forming enzymes, such as aminoacyl-tRNA synthetases,
Keywords
acyl-CoA synthetase; enantiomer; fire y
luciferase; kinetic resolution; nonsteroidal
anti-inflamatory drugs
Correspondence
D ... kinds of hydrolytic conditions, chemical
hydrolysis under high pH and enzymatic hydrolysis
under neutral pH, were examined, because there was
some fear of racemization by the chemical hydrolysis.
The ... Identification of 2-arylpropanoyl-CoA produced by LUC-H
with TOF-MS analysis. For the detection of 2-arylpropanoyl-CoAs
by TOF-MS analysis, see the Experimental procedures section.
Entry Thioester
Predicted
[M-H]
–
Observed
[M-H]
–
Error
(p.p.m.)
1...
... data). Tryptophan and tyro-
sine residues (Y1 7, Y2 0, Y8 2, W84, and W369) in CPY
would be perturbed by the carbohydrate moiety, thus
increasing their fluorescence, since they are in close proximity
to ... 2562–2567.
Table 1. Summary of qualitative thermodynamic parameters for the temperature- and pressure-induced transitions of CPY, Dgly CPY, proCPY and
Dgly proCPY calculated by fluorescence analysis. Errors ... pro-
nounced hydration upon unfolding of the unglycosylated
form.
At high pressure, in the glycosylated forms the carbohy-
drate moiety of CPY and proCPY would be hydrated to
compensate the volume contraction...
... 6110.
Abbreviations:ATEE,N-acetyl-
L
-tyrosine ethyl ester monohydrate;
BTEE, benzoyl-
L
-tyrosine ethyl ester; CPY, carboxypeptidase Y;
GdnHCl, guanidine hydrochloride.
*Present address: Sanyo Chemical Co., Kyoto, Japan.
Present ... of carbohydrate
moiety in carboxypeptidase Y: structural study of mutant enzyme
lacking carbohydrate moiety. Biochem. Biotechn Biosci. 63, 1045–
1050.
2. Jung, G., Ueno, H. & Hayashi, R. ... cleft of CPY is constructed with five a-helices,
these locate as if they surround the catalytic triad. In
addition, five disulfide bridges (Cys56–298, Cys198–207,
Cys217–240, Cys224–233 and Cys262–268)...
... Gorrell
1
1 Centenary Institute, Sydney Medical School, University of Sydney, NSW, Australia
2 Pharmaceutical Chemistry, Faculty of Pharmacy, University of Sydney, NSW, Australia
Keywords
antiplasmin-cleaving ... pmolặmin
)1
ặlg
)1
on benzyloxycar-
bonyl (Z)-Gly-Pro-AMC and 1830 nmolặmin
)1
ặlg
)1
on
H-Gly-Pro-p-nitroanilide, respectively. To assay the
substrate specificity of each protease, enzyme activity
assays were ... megakaryocytes, adipose tissue and gut [45,46].
Dipeptidyl peptidase truncation of NPY by FAP or
DPP4 yields NPY
3–36
. NPY
3–36
is inactive on the Y1
receptor, and has greater affinity for the Y2 ...
... periosteum and
bone marrow by RIA [13], particularly in megakaryo-
cytes [14]. Recently, it was additionally reported that
the NPY receptor Y1 , but not Y2 , Y4 , Y5 or Y6 , was
expressed in cultured ... the adult, that NPY is synthesized by
osteoblasts, osteocytes, and chondrocytes. Moreover, peptidylglycine a-am-
idating monooxygenase, the enzyme responsible for NPY activation by
amidation, was ... activity (nmolPNP·mg·h
–1
)
6
4
2
0
25
20
15
10
10
8
6
4
2
0
0
actin GAPDH HPRT
GAPDH
HPRT
2
4
6
8
10
12
14
b
a
a
5
0
Day 3
Day 3
Day 7
Day 7
c
b
b
TTR KO
TTR KO
WT
Day 14
Day 3 Day 7
Day 14
Day 14
WT
WT
TTR...
... polymerization
activity.
Fidelity analysis of AtPOLK and AtPOLK
n
C
We next analysed the catalytic efficiency and fidelity of
AtPOLK and AtPOLKDC during DNA synthesis. To
determine the fidelity of both full-length ... polymerase targeting and recruitment, our
understanding of its role in Y- family DNA poly-
merase catalytic activity is still limited. Efforts to
relate the enzymatic properties of Y- family DNA ... (2001) The Y- family of DNA
polymerases. Mol Cell 8, 7–8.
5 Yang W (2005) Portraits of a Y- family DNA poly-
merase. FEBS Lett 579, 868–872.
6 Kunkel TA (2004) DNA replication fidelity. J Biol
Chem...
... assay based on LUC biolumin-
escence reaction is an important analytical tool, mainly
Keywords
Coenzyme A; dehydroluciferyl-adenylate;
dehydroluciferyl-coenzyme A; dephospho-
coenzyme A; fire y ... A; L, dehydroluciferin; L-AMP, dehydroluciferyl-adenylate;
LUC, fire y luciferase; L-CoA, dehydroluciferyl-coenzyme A; L-dephospho-CoA, dehydroluciferyl-dephospho-coenzyme A; LH
2
, fire y luciferin;
LH
2
-AMP, ... enzyme produced fire y luciferyl-adenylate
and the enzyme synthesis of luciferyl-CoA. Chembio-
chem 5, 110–115.
22 Hosaka K, Mishina M, Tanaka T, Kamiryo T & Numa
S (1979) Acyl-coenzyme A synthetase...