automatic identification of problematic situations
Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt
Ngày tải lên :
15/02/2014, 01:20
... form
Parallel form
Fig. 5. CD of Htelo in the presence of various amounts of RGG3.
Titration of Htelo with RGG3 (1.2, 1.0, 0.8, 0.6, 0.4, 0.2, 0.1 and 0
equiv. of RGG3) in 100 m
M KCl and 50 ... amounts of RGG3.
The CD spectrum of Htelo, a hybrid (3 + 1) form,
showed a strong positive band at 290 nm and a nega-
tive band at around 235 nm, whereas the addition of 1
ratio excess of RGG3 ... 250
0.8
1
0.6
0.4
0.2
0
0.8
1
Fraction of RNA bound
Fraction of DNA bound
Fig. 4. Binding affinity of RGG3 to Htelo or
rHtelo. The DNA or RNA concentration was
fixed at 1 n
M, whereas the concentration of
RNase-treated...
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Tài liệu Báo cáo khoa học: The cellulosomes from Clostridium cellulolyticum Identification of new components and synergies between complexes ppt
Ngày tải lên :
18/02/2014, 08:20
... percentage of amino acid coverage in the matched proteins; M
pep
, the number of unique matched peptides; U
pep
, the number of
unmatched peptides in the MALDI-TOF experiments. The function of new ... spectrum of protein 5, fraction 5 ⁄ fraction 6.
Fig. S7. MS spectrum of protein 8, fraction 5 ⁄ fraction 6.
Fig. S8. MS spectrum of protein 11, fraction 5 ⁄ fraction 6.
Fig. S9. MS spectrum of protein ... patterns
of activity [13,23]. It has been suggested that the pres-
ence of several well-separated peaks in the case of cell-
ulosomes from C. cellulovorans may be partly due to
the existence of various...
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Tài liệu Báo cáo khoa học: Identification of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc
Ngày tải lên :
18/02/2014, 14:20
... four molecules of 3OH-C12:0, two of C10:0,
and one of C12:0 [10]. When compared to this struc-
ture, the lipid A of O35ElpxX lacks two decanoic acyl
(C10:0) substituents, and that of O35ElpxL lacks ... NaCl ⁄ chloroform (1 : 1
v ⁄ v). A new volume of chloroform was used for each of
the three extractions. Finally, the organic phase was
re-extracted with de-ionized water to remove traces of salts,
and ... Analysis of physio-
chemical and biological features of both mutants was
performed to study the functions of these genes and
the structures of their resultant LOSs in vitro and
in vivo.
Results
Identification...
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Tài liệu Báo cáo khoa học: Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress docx
Ngày tải lên :
18/02/2014, 16:20
... in mantle. One
inducible and two constitutive isoforms of HSP70 are
synthesized in the gills and mantle of C. gigas [26].
The expression level of the constitutive forms increases
after thermal ... recovery of
cells after protein damage, by assisting the folding of
cytoskeletal proteins that are actively synthesized
and ⁄ or renatured under these conditions. The upregu-
lation of all of these ... protein L10, is a transcription cofactor that
inhibits activation of AP-1 transcription factors. QM
protein is implicated in the conversion of a broad vari-
ety of extracellular signals generated...
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Tài liệu Báo cáo khoa học: Suppressed catalytic efficiency of plasmin in the presence of long-chain fatty acids Identification of kinetic parameters from continuous enzymatic assay with Monte Carlo simulation ppt
Ngày tải lên :
18/02/2014, 17:20
... of plasmin in the amidolytic
assay. However, if we use the k
p
⁄ K
m
ratio as a mea-
sure of the overall impact of stearate, it should be clas-
sified as an inhibitor of plasmin, the potency of ... effect of
random outliers. The probability distribution of the
parameters estimated using this robust evaluation
procedure was essential for the identification of the
statistical significance of the ... Light attenuation of oleic acid micelles in the presence of synthetic plasmin substrate. (A) Twenty microlitres of 1 mM Spectrozyme-PL
was added to 180 lL of various concentrations of oleic acid...
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Tài liệu Báo cáo khoa học: Identification of a novel matrix protein contained in a protein aggregate associated with collagen in fish otoliths pdf
Ngày tải lên :
18/02/2014, 17:20
... Localization of OMM-64 in the inner ear sacculus by immu-
nohistochemistry. (A) Macular region of the sacculus after removal
of the otolith. Strongly immunoreactive cells were observed at the
periphery of ... of the antibody was confirmed by the detection of
no bands in the supernatant SDS–PAGE. Subsequently,
100 lL of saccular extract or water-soluble otolith matrix
proteins were bound to 10 l L of ... cut-off). Production of
rabbit antiserum raised against the recombinant protein
and affinity purification of the antibody were performed by
Hokkaido System Science (Hokkaido, Japan).
Collection of...
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Tài liệu Báo cáo khoa học: Direct identification of hydrophobins and their processing in Trichoderma using intact-cell MALDI-TOF MS docx
Ngày tải lên :
19/02/2014, 02:20
... MALDI-TOF spectra of mycelia (A, C, E) and sporulating mycelia (B, D, F) of three strains of H. jecorina grown on malt
agar. The masses displayed have an error of about 0.1%, so peaks of 7232 ... for the rapid detection of hydrophobins
from a large number of small samples, we have
investigated the potential of intact-cell MALDI-TOF
MS (ICMS). Initial applications of ICMS on fila-
mentous ... typical MALDI-
TOF mass spectra of four strains of H. koningii, T. long-
ibrachiatum, H. virens and T. atroviride. The spectra
Fig. 1. Alignment of the six hydrophobin protein sequences of H. jecorina....
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Tài liệu Báo cáo khoa học: Identification of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx
Ngày tải lên :
19/02/2014, 07:20
... exhibit a wide range of structural diversity produced by a
sequence of biosynthetic reactions. Cyclization of oxidosqualene is the ini-
tial origin of structural diversity of skeletons in their ... min.
After the addition of 0.25 mL of 40% potassium hydroxide
solution and 0.25 mL of methanol, the cell suspension was
boiled for 5 min. Products were extracted three times with
0.2 mL of hexane and evaporated. ... addition of
0.1 mL each of methanol and water. The products were
extracted three times with hexane (0.2 mL). After evapor-
ation, the residue was dissolved in 0.02 mL of hexane, and
0.001 mL of hexane...
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Tài liệu Báo cáo khoa học: Identification of GAS-dependent interferon-sensitive target genes whose transcription is STAT2-dependent but ISGF3-independent doc
Ngày tải lên :
19/02/2014, 07:20
... number of genes in U6A
and U6A-2 cells, IFN-treated U6A-VV-II cells showed
the down-regulation of a substantially larger subset of
genes.
Identification and characterization of a subset of
ISGF3-independent ... 1579
expression profile of cells expressing the VV-II mutant
form of STAT2 was more similar to that of U6A cells
than that of U6A-2 cells (Level 1). IFN treatment led
to the further segregation of the sample ... Department of Cell and Molecular Biology, Toronto General Research Institute, University Health Network, and Department of
Immunology, University of Toronto, ON, Canada
2 Division of Signaling...
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Tài liệu Báo cáo khoa học: Identification of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1/angiomodulin/mac25) doc
Ngày tải lên :
19/02/2014, 07:20
... Rosenfeld RG (1997) Identification of a family of
low-affinity insulin-like growth factor binding proteins
(IGFBPs): characterization of connective tissue growth
factor as a member of the IGFBP superfamily. ... the
presence of 10 mm CaCl
2
, as described in Experimental
procedures. Of the nine cell lines tested, OVISE,
Fig. 1. Analysis of noncleaved and cleaved
forms of IGFBP-rP1 in conditioned media of
eight ... enzyme of IGFBP-rP1. On the
other hand, soluble forms of matriptase, which are
released from cell membranes by proteolysis, were
detected in the conditioned media of at least 5 cell
lines out of...
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Tài liệu Báo cáo khoa học: Bioinformatics of the glycoside hydrolase family 57 and identification of catalytic residues in amylopullulanase from Thermococcus hydrothermalis doc
Ngày tải lên :
19/02/2014, 12:20
... in the absence of azide.
Ó FEBS 2004 Bioinformatics and mutagenesis of GH-57 (Eur. J. Biochem. 271) 2869
Bioinformatics of the glycoside hydrolase family 57 and
identification of catalytic residues ... study of GH-57, no detailed
alignment of the complete sequences of GH-57 members
has yet been accomplished. To date, only p artial or selected
sequences have been compa red [17–19]. An alignment of
GH-57 ... AL662. The analysis
of this sequence revealed that the encoded enzyme i s a
member of GH-57 [21,22]. The cloning and expression of apu
in Escherichia c oli has led to the production of a C-terminally
truncated...
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Tài liệu Báo cáo khoa học: What makes biochemical networks tick? A graphical tool for the identification of oscillophores ppt
Ngày tải lên :
19/02/2014, 16:20
... sign) of a cycle is
the product of the values (the signs) of its steps. The sign o f
the magnitude of a Ôminus combined autoinfluence path
of order pÕ equal those of the arithmetic product of its
Ó ... absolute magnitude of a
combined autoinfluence path is the product of all the rates
divided by the product of all the concentrations of its
species. Therewith the c oefficient of o rder p of the
characteristic ... the basis of the graphical analyses of the
characteristic equation and of t he method we develop here.
Inspection of Eqn 12 shows t hat in a ll coefficients a
p
the
term consisting of negative...
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Tài liệu Báo cáo khoa học: Identification of ATP-NADH kinase isozymes and their contribution to supply of NADP(H) in Saccharomyces cerevisiae docx
Ngày tải lên :
20/02/2014, 01:20
... interaction of Yef1p with Utr1p is of
biological interest and may be related to the pro-
nounced requirement of both Yef1p and Utr1p in the
absence of Pos5p.
The elucidation of the localization of Utr1p ... The loss of YCp-UTR1 was con-
firmed by the Ura– phenotype of MK1219 (require-
Table 2. Effect of compounds on ATP-NAD kinase activity of
Yef1p. The effect of compounds on the activity of Yef1p ... abilities of LEU2, UTR1 and YEF1
for the growth defects of the pos5 mutant
To confirm the complementing ability of LEU2 for the
growth defects of the pos5 mutant, we examined the
growth of pos5...
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Tài liệu Báo cáo khoa học: Identification of critical active-site residues in angiotensin-converting enzyme-2 (ACE2) by site-directed mutagenesis docx
Ngày tải lên :
20/02/2014, 01:20
... effect of chloride ions on enzyme activity
was investigated (Fig. 4). The hydrolysis of Mca-
Fig. 3. Role of His505 and His345 in catalysis. Schematic of the
proposed reaction intermediate of ACE2, ... study has highlighted the importance of Arg273
in binding of the C-terminus of peptide substrates by
ACE2. The complete loss of activity observed as a
result of replacing this single residue could ... nitrogen) of both residues are
within hydrogen-bonding distance of the carbonyl
oxygen of the amide group of residue P1¢ (tACE)
(Fig. 1B) or equivalent terminal carboxylate oxygen
(ACE2) (Fig. 1A) of...
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Tài liệu Báo cáo khoa học: Identification of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically binding to aptameric cytotoxic GT oligomers ppt
Ngày tải lên :
21/02/2014, 00:20
... Identification of different isoforms of eEF1A in the nuclear fraction
of human T-lymphoblastic cancer cell line specifically binding
to ... the mechanism of action
of our cytotoxic GT oligonucleotides.
In this article, we report the identification of eukaryotic
elongation factor 1 alpha (eEF1A) as a nuclear component
of the CRC in ... the
quantitation of microgram quantities of protein utilizing the
principle of protein-dye binding. Anal. Biochem. 72, 248–254.
20. Laemmli, U.K. (1970) Cleavage of structural proteins during the
assembly of...
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