... ruling passion." Great as was the intellect of Darwin, his character, as Huxley wrote, was even nobler than his intellect A. C SEWARD Botany School, Cambridge, March 20, 1909 CONTENTS PREFACE DATES ... simple, cordial and unpretending than the encouragement which he afforded to all young naturalists." "During the three years which I spent at Cambridge my time was wasted, as far as the academical ... undertook the translation of the essays by Prof Weismann and Prof Schwalbe; Mrs James Ward was good enough to assist me by translating Prof Bougle's article on Sociology, and to Mr McCabe I am indebted...
... display a characteristic biphasic IN-DNA dissociation which is detected by SPR as a fast phase followed bya much slower dissociation The dissociation kinetics of the I26 7A and I26 7A/ I26 8A derivatives ... BIOTIN-GCACACCTTTTAGAGATCGTCA-5' The author(s) declare that they have no competing interests Acknowledgements (3) TARGET (non-viral DNA sequence): top strand, 5'CCGCGATAAGCTTTAATGCGG-TAG-3'; bottom strand, ... binding was not accompanied by an increase in -2 processing but rather by an increase in nonspecific DNA cleavage with these derivatives (Figure 3) A rapid association with DNA, coupled with the fast...
... 2499–2513 Kanda N, Seno H, Konda Y, Marusawa H, Kanai M, Nakajima T, Kawashima T, Nanakin A, Sawabu T, Uenoyama Y et al (2004) STAT3 is constitutively activated and supports cell survival in association ... carcinoma tumors and cell lines: inhibition of JAK3 ⁄ STAT3 signaling induces apoptosis and cell cycle arrest of colon carcinoma cells Am J Pathol 167, 969–980 Kunnumakkara AB, Anand P & Aggarwal ... triplicate Subcellular location of phospho-STAT3 analysed by fluorescence microscopy: (B) in nontreated cells, phophoSTAT3 was cytoplasmic and nuclear; (C) in hpST3dODN-treated cells, STAT3 was almost...
... oxygens and free radicals did not participate in the TS effect, and that the inhibitory effect of a- T was mediated bya nonantioxidative reaction a- T was reported to decrease PKCa activity due to activation ... Nishioka, Y., Kitahara, M., Sakashita, M & Tanaka, S (1994) Lysophosphatidic acids induce proliferation of cultured vascular smooth muscle cells from rat aorta Am J Physiol 267, C2 04 C2 10 27 ... nitrite as a standard Western blotting of inducible NO synthase Fig Structures of a- tocopherol (a- T) and T derivatives a- tocopheryl hemisuccinate (TS), a- tocopheryl acetate (TA) and a- tocopheryl nicotinate...
... trifluoroethanol Analytical ultracentrifugation Analytical ultracentrifugation was carried out on a Beckman Optima XL -A ultracentrifuge at 20 C according to the manufacturer’s instructions Protein concentration ... inert HPLC system (Merck-Hitachi) using a C8 reverse-phase column (Vydac) The solvent gradient was produced from degassed HPLCgrade water containing 0.07% trifluoroacetic acid and degassed acetonitrile ... purification Trifluoroethanol and cytidine 2¢:3¢-cyclic monophosphate (cCMP) were from Fluka, phenylmethanesulfonyl fluoride was from Merck, and N-succinyl-Ala-Ala-Alap-nitroanilide from Bachem All...
... derived from CCR5, a chemokine receptor, which plays a crucial role in HIV infection In all of these catalytic antibodies, a catalytic triad composed of Asp, Ser, and His was always identified ... Mitsuda Y, Hifumi E, Tsuruhata K, Fujinami H, Yamamoto N & Uda T (2004) Catalytic antibody light chain capable of cleaving a chemokine receptor CCR-5 peptide with a high reaction rate constant ... isolated heavy Catalytic features of anti-HpU-9 mAb light chain chain HpU-9-H, which was prepared by exactly the same purification steps as those for HpU-9-L, also failed to cleave the antigenic...
... oligonucleotides 5¢-CTAGACCA CCATGGAGGAACAGAAGCTGATCAGTGAGGAAG ACCTGGATATCCCGGGTTAACT-3¢ and 5¢-CTAGAG TTAACCCGGGATATCCAGGTCTTCCTCACTGATCA GCTTCTGTTCCTCCATGGTGGT-3¢, and 5¢-CTAGAC CACCATGGACTACAAAGACGATGACGATAAAGAT ... CAATCTCCCATCCGTTGATGTG-3¢, and pcerulean-N1 pBOS-HA was constructed by insertion of the annealed fragment of the synthesized oligonucleotides, 5¢-CTAGAC CACCATGTACCCCTACGACGTGCCCGACTACGCCG ATATCCCGGGTTAACT-3¢ ... was changed to GGA by using primers 5¢-CCCAAGC TTATGGATGGAGGAGGAGAAAAC-3¢ and 5¢-ACGT ACCGGTCCACAATCTAGGAAGTTTGCAGC-3¢ After digestion of the PCR fragment by EcoRI and AgeI, the fragment was inserted...
... sequences are: ZIP8 #1, accuaaagca uuaccugccaucaau; ZIP8 #2, ccgauuucaccuucuucaugauuca; ZIP8 #3, ggauuccugucagugacgauuauua; eGFPsi, gcaagcuga cccugaaguucau; PKCa #1, ccaucggauuguucuuucuucauaa; PKCa ... PKCa #2, gccuccauuugauggugaagaugaa; PKCd #1, ccacu acaucaagaaccaugaguuu; and PKCd #2, ccauccacaagaaaugc aucgacaa PKCe #1, PKCe #2 and PKC siRNAs are the ‘validated Stealth RNAi duo pak’ from Invitrogen ... 1673 Cd-induced ERK activation is inhibited by PKC A P Martin et al B C Fig Quantification of cadmium accumulation in cells (A) Comparison of intracellular cadmium accumulation from medium containing...
... native TrIFN -c was measured by gel-filtration, in comparison with those of crude LeIFN -c and unglycosylated rIFN -c Each column fraction was tested by antiviral assay and by IFN -c speci c ELISA ... a day-15 pregnant gilt (B) crude natural LeIFN -c (C) recombinant IFN -c (E.coli) In each fraction IFN -c concentration (d) was measured by ELISA, and antiviral activity (h) was determined by antiviral ... that the structural and chemical characteristics of TrIFN -c affects its bioavailability and biological effect(s) on the maternal uterus In particular, this shortened version of IFN -c, lacking a...
... TTCCACGCGAAGTTATGCACTTTCAT-3¢, 5¢-AATTTC TTCAGTGGCTTTCGCCACATTGAAAAAATCGGT-3¢, 5¢-GATCCTTTCAGCGAGATCCGCAAACATGTCCGC AAACTC-3¢ and 5¢-TTGCAGCATCCAAATGGACGCTT GCAACTTGGCCAATTG-3¢ for H2 5A, H3 7A, D5 2A and K13 4A, respectively ... pET2 9c vector, using the primers HPNAP 1–57-up (5¢-GCGGAATTCCATATGAAAA CATTTGAAATT-3¢) and HPNAP 1–57-low (5¢-CCGCTC GAGCCTTTCAGCGA-3¢) (XhoI), and HPNAP 58–144up (5¢-GCGGAATTCCATATGATCGTTCAATTAGGA3¢) ... pET-1 1a and pET2 9c The hpnap gene was amplified by PCR from the H pylori J99 genome using primers HPNAP_up (5¢-GCGGAA TTCCATATGAAAACATTTGAAATT-3¢) and HPNAP_ low (5¢-GCGGGATCCTTAAGCCAAATGGGCTTG-3¢),...
... h Cells were collected and stained with propidium iodide according to the standard protocol of the FACS core facility (UNMC, Omaha, USA) The cell cycle was analyzed bya BD FACSCalibur flow cytometer ... immunohistochemistry J Clin Oncol 2006, 24:816-822 Andrade CR, Takahama Junior A, Nishimoto IN, Kowalski LP, Lopes MA: Rhabdomyosarcoma of the head and neck: a clinicopathological and immunohistochemical analysis ... therapeutic target in alveolar and embryonal rhabdomyosarcoma Cancer Res 2006, 66:4742-4749 36 Puri N, Ahmed S, Janamanchi V, Tretiakova M, Zumba O, Krausz T, Jagadeeswaran R, Salgia R: c- Met is a potentially...
... h Cells were collected and stained with propidium iodide according to the standard protocol of the FACS core facility (UNMC, Omaha, USA) The cell cycle was analyzed bya BD FACSCalibur flow cytometer ... immunohistochemistry J Clin Oncol 2006, 24:816-822 Andrade CR, Takahama Junior A, Nishimoto IN, Kowalski LP, Lopes MA: Rhabdomyosarcoma of the head and neck: a clinicopathological and immunohistochemical analysis ... therapeutic target in alveolar and embryonal rhabdomyosarcoma Cancer Res 2006, 66:4742-4749 36 Puri N, Ahmed S, Janamanchi V, Tretiakova M, Zumba O, Krausz T, Jagadeeswaran R, Salgia R: c- Met is a potentially...