and scanner precision in vivo and in vitro
Tài liệu Báo cáo khoa học: The endogenous retinoid metabolite S-4-oxo-9-cis-13,14-dihydro-retinoic acid activates retinoic acid receptor signalling both in vitro and in vivo pdf
Ngày tải lên :
18/02/2014, 08:20
... ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) and 1% nonessential amino acids (Gibco Invitrogen) Murine hepatoma-1 ... all-trans-retinoic acid, the findings clearly demonstrate that S-4o9cDH-RA has the capacity to bind and activate nuclear retinoid receptors and regulate gene transcription both in vitro and in vivo Retinoids ... grown in low-glucose DMEM (Gibco Invitrogen) supplemented with 10% (v ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) and...
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Báo cáo khoa học: Two types of replication protein A in seed plants Characterization of their functions in vitro and in vivo ppt
Ngày tải lên :
07/03/2014, 21:20
... USA) and transformed into E coli for protein induction Extracts prepared from cells induced for h were shown to contain a six-histidine C-terminal-tagged OsRPA14 fusion protein The OsRPA14 protein ... tag were obtained by in vitro translation in wheatgerm extract (Invitrotec) according to the manufacturer’s procedure OsRPA70a (or OsRPA70b) and OsRPA32, or OsRPA32 and OsRPA14 were incubated with ... soluble in the presence of m urea Soluble fractions were loaded on to columns containing 10 mL His-Bind resin (Novagen) The columns were washed with 50 mL binding buffer containing m urea and then...
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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
Ngày tải lên :
07/03/2014, 21:20
... shown) In conclusion, we have shown using both in vitro and in vivo methods, that PSI-G adopts a topology in the thylakoid membrane with the loop facing the stroma and its N- and C-termini facing ... promoter and terminator, and into vectors for in vitro transcription and translation An Arabidopsis line (psag-1.4 [5], termed DG in this report), lacking PSI-G due to a transposon footprint in exon ... after 0, 30 and 60 digestion with trypsin, probed with antibodies directed against PSI-G, PSI-K (intrinsic membrane protein, stromal loop), PSI-O (intrinsic membrane protein, luminal loop) and PSI-D...
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Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx
Ngày tải lên :
23/03/2014, 13:20
... CPS and O-chain LPS are composed of linear trisaccharide repeating units containing 3-linked 2-acetamido-2-deoxy-D-quinovose, 4-linked 3-[(N-acetyl-L-alanyl) amido]-3-deoxy-D-quinovose and 3-linked ... Interresidue NOEs were observed between H-1a and H-3c and between H-1b and H-3a, suggesting a linear structure a-c-b (Fig 2E,F) In addition, interresidue NOEs between H-1c and H-4b (Fig 2G) indicated ... and O-chain polysaccharide of A salmonicida strain 80204-1 the previously identified O-chain containing L-rhamnose, 2-amino-2-deoxy-D-mannose and D-glucose [11], confirming that the novel CPS and...
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Báo cáo khoa học: In vitro and in vivo self-cleavage of Streptococcus pneumoniae signal peptidase I pot
Ngày tải lên :
23/03/2014, 21:21
... the protein The self-cleavage of LexA protein is important in the SOS response in bacteria [21–23] In vitro self-cleavage was also observed in all investigated bacterial SPase I including enzymes ... results intrigued us to investigate the cleavage of S pneumoniae SPase I in vivo As shown in Fig 6C, Western blot analysis demostrated that the self-cleavage of SPase I is indeed occuring in vivo in ... product in exponential growth phase In general, the bacterial cells maintained the active SPase I in the highest level in Fig Cell growth of S pneumoniae and Western blot analysis of in vivo self-cleavage...
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Báo cáo khóa học: Determination of modulation of ligand properties of synthetic complex-type biantennary N-glycans by introduction of bisecting GlcNAc in silico, in vitro and in vivo pot
Ngày tải lên :
30/03/2014, 13:20
... nonspecific binding by protein–protein interactions, cells were incubated with 100 lg ligand-free carrier protein (BSA) per mL for 30 at °C before incubation with the biotinylated neoglycoprotein in Dulbecco’s ... assay, saturable and carbohydrate-dependent binding was measured, and the resulting Scatchard plots gave straight lines indicating the presence of a single class of binding sites in each case (not ... neoglycoproteins, we determined their binding to cells and to tissue sections as well as their biodistribution in mice after injection of radioiodinated material Binding to cells and organs In these...
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Báo cáo hóa học: " Vaccinia virus lacking the deoxyuridine triphosphatase gene (F2L) replicates well in vitro and in vivo, but is hypersensitive to the antiviral drug " pot
Ngày tải lên :
20/06/2014, 01:20
... evaluated in cell culture and in intranasally infected mice The deletion of F2L had a minimal impact on viral replication in vitro and did not appear to significantly reduce the virulence in vivo ... repeatable increase in the efficacy of N-methanocarbathymidine (N-MCT) (Table 2) This compound is a carbocyclic thymidine analog that inhibits the replication of VV both in vitro and in vivo [12,13], and ... differences in replication in mice reflect real differences in the biology of these viruses, or are related to differences in the animal models including route of infection In HSV, direct intracranial inoculation...
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Báo cáo hóa học: " N-methylisatin-beta-thiosemicarbazone derivative (SCH 16) is an inhibitor of Japanese encephalitis virus infection in vitro and in vivo" ppt
Ngày tải lên :
20/06/2014, 01:20
... reports describing the in vitro inhibitory effect of compounds such as ribavirin, mycophenolic acid, imino sugars, inhibitors of serine protease, RNA interference and non-steroidal anti-inflammatory ... lanes and – in vitro translation products of RNA obtained form JEV infected PS cells (untreated) at and 10 hours post infection respectively Lanes and – in vitro translation product of RNA obtained ... Comparative study of the effects of isatin, an endogenous MAO-inhibitor, and seleginine on bradykinasia and dopamine levels in a rat model Parkinson's disease induced by the Japanese encephalitis...
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Báo cáo hóa học: " Inhibition of foot-and-mouth disease virus replication in vitro and in vivo by small interfering RNA" pot
Ngày tải lên :
20/06/2014, 01:20
... J, Ince N, Ouyang N, Min J, Chen J, Shankar P, Lieberman J: RNA interference targeting Fas protects mice from fulminant hepatitis Nat Med 2003, 9:347-351 Gitlin L, Karelsky S, Andino R: Short interfering ... growing in a monolayer, and having a well-defined tendency for parallel orientation Viral infection causes a marked CPE resulting in total cellular detachment, rounding, and destruction, which ... 36 and 48 h postinfection Samples of supernatant were obtained at designated time points, and the TCID50 were determined by the Reed-Muench formula BHK-21 cells are fibroblastic, growing in a...
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báo cáo hóa học:" Functional significance of the signal transduction pathways Akt and Erk in ovarian follicles: in vitro and in vivo studies in cattle and sheep" ppt
Ngày tải lên :
20/06/2014, 07:20
... in mediating the effects of FSH on inhibin-A, activin-A, oestradiol and progesterone secretion and also important in mediating IGF-I stimulated inhibin-A, activin-A, follistatin, oestradiol and ... previously in the bovine model Increases in Akt and Erk signalling proteins in response to FSH and IGF stimulation suggest a role for Akt and Erk signal transduction pathways in FSH and IGF mediated ... progesterone and androstenedione in bovine theca cells in vitro[ 38] The results from Experiment clearly indicate that treatment of follicles in vivo with inhibiters of the Akt and Erk pathways in the...
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báo cáo hóa học: " In vitro and in vivo pre-clinical analysis of a F(ab’)2 fragment of panitumumab for molecular imaging and therapy of HER1-positive cancers" pot
Ngày tải lên :
21/06/2014, 02:20
... distribution in these last two studies was similar to what was obtained with the i.v injected 11 1In- labeled panitumumab F(ab’)2 already discussed Discussion The in vivo and in vitro properties of the intact ... radiolabelings TN performed PET imaging and analysis MWB synthesized the chelate and participated in the design of the studies DEM conceived of and coordinated the studies and assisted with the in vivo ... 48-96 h post-injection [13] The objective of this study was to evaluate the in vivo and in vitro properties of panitumumab F(ab’)2 fragment and to assess its utility as a targeting agent in radioimmunodiagnostic...
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Báo cáo hóa học: " CdTe quantum dots with daunorubicin induce apoptosis of multidrug-resistant human hepatoma HepG2/ADM cells: in vitro and in vivo evaluation" pptx
Ngày tải lên :
21/06/2014, 03:20
... cultured in the cell culture medium containing μg/ mL adriamycin (Sigma) Both cell lines were maintained in RPMI-1640 medium containing 10% FCS, 100 U/ml of penicillin, and 100 μg/ml of streptomycin ... and fixed in 100% methanol for 10 Cell monolayers were blocked in 5% BSA in PBS for 45 and incubated for h at room temperature with P-gp antibodies (Invitrogen, Beijing, China), followed by incubation ... were suspended in 100 μL of culture medium and subcutaneously inoculated into the right flank of mice using a 1.0 mL syringe Intravenous injection of reagents and tumor growth inhibition study...
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Probiotic Lactobacillus Strains: in vitro and in vivo Studies pot
Ngày tải lên :
07/07/2014, 19:20
... immunomodulating properties in in vivo mouse model MATERIAL AND METHODS Survival of Lactobacillus strains at low pH and in the presence of bile salt Lactobacillus strains isolated from feces of healthy infants ... 2000) Experimental design in vivo Eight-week-old inbred BALB/c mice (n = per group) were used for in vivo experiments Strains selected in in vitro tests were mixed in equal proportions, lyophilized ... Serum and Vaccine Producer Biomed, Cracow, Poland), Salmonella enterica subsp enterica sv Enteritidis (clinical isolate obtained from the Institute of Sea and Tropical Medicine, Gdynia, Poland),...
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Báo cáo sinh học: " CNS progenitor cells and oligodendrocytes are targets of chemotherapeutic agents in vitro and in vivo" pdf
Ngày tải lên :
06/08/2014, 18:21
... provide rapid in vitro screens for analyzing new therapies and discovering means of achieving selective protection or targeted killing In light of the ease of use of these in vitro and in vivo assays, ... TUNEL staining, doublecortin staining, S-100b staining, and the merged image (a-d) Images taken at -4 µm; (e-h) -2 µm; (i-l) µm; (m-p) µm The congruence between the doublecortin+ staining and the ... with cytarabine leads to increased and prolonged cell death, and decreased BrdU incorporation, in the adult mouse CNS Cell death and BrdU incorporation were examined as in Figures and (a) The...
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Báo cáo khoa học: "In vitro and in vivo gene therapy with CMV vector-mediated presumed dog b-nerve growth factor in pyridoxine-induced neuropathy dogs" pot
Ngày tải lên :
07/08/2014, 23:22
... pyridoxine intoxication in dogs In high doses, pyridoxine causes a selective degeneration of large and small myelinated sensory axons in the central and peripheral nerves, resulting in numbness and ... of pyridoxine injection On the fourth day of pyridoxine injection, all dogs held their hindlimb stiffly when standing These conditions were maintained until the end of the pyridoxine injection ... Mata M, Fink DJ In vivo gene therapy for pyridoxine-induced neuropathy by herpes simplex virus-mediated Presumed dog β-NGF gene therapy in vitro and in vivo 373 gene transfer of neurotrophin-3 Ann...
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Báo cáo khoa học: "Effects of sustained subculture on apparent rejuvenation of the apple rootstock M.9 in vitro and in vivo" pps
Ngày tải lên :
09/08/2014, 02:21
... expressed as improved shoot production and rooting in vitro and subsequently of micropropagated plants in the field Similar increased rooting ability of cuttings from micropropagated stockplants ... sustained for at least yr (Howard et aL, 1989a) Differences in shoot production and rooting of M.9 in vitro also appeared to originate from differences between explants used to initiate culture lines ... proliferation medium improved subsequent rooting at the 11th and l5th subcultures but not at the 21 st subculture plantlets in compost following in vitro rooting but was increased significantly (P
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Báo cáo y học: "Inhibition of macropinocytosis blocks antigen presentation of type II collagen in vitro and in vivo in HLA-DR1 transgenic mice" docx
Ngày tải lên :
09/08/2014, 08:22
... importance of our finding that blockade of CII uptake prevents activation of specific T cells in vitro was tested in vivo We administered amiloride in vivo and showed reduction in the magnitude ... temperature and permeabilized in 0.1% Triton X-100 in PBS for 15 minutes at 4°C After washing (10 mM TRIS HCl pH 7.6, containing 150 mM NaCl, TBS buffer) and blocking (normal rabbit serum 1:5 in TBS ... data and error bars denoting 95% confidence interval are presented effect of amiloride and cytochalasin D was independent of NFκB inhibition Dose-response data obtained in the absence of inhibitors...
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Báo cáo khoa học: "Radiosensitization and growth inhibition of cancer cells mediated by an scFv antibody gene against DNA-PKcs in vitro and in vivo" docx
Ngày tải lên :
09/08/2014, 09:20
... effective in inhibiting the function of proteins by blocking the activity domain or critical protein interaction sites There have been numerous antibody drugs undergoing pre-clinical and Du et ... DNA-PKcs is approximately 470 kD consisting of 4128 amino acids Its C-terminus contains a serine/threonine protein kinase domain (3719- 4128) [23] For screening Page of 11 the specific anti-DNA-PKcs ... protein level in HeLa, HeLa-pcDNA and HeLa-DPK3-scFv cells (B) In vitro detection of Gy-induced DNA-PKcs activity using the Signa-TECT® DNA-Dependent Protein Kinase Assay System Immunoblotting...
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Báo cáo khoa học: "Tumor response to radiotherapy is dependent on genotype-associated mechanisms in vitro and in vivo" ppsx
Ngày tải lên :
09/08/2014, 09:20
... and maintained data records CK and JBL were members of the external advisory committed and worked with JW in planning and interpreting the studies JFD confirmed dosimetry and treatment planning ... 19S186) and two cell lines shown in table to express diminished p21 (SW1222 cells and U251 cells) Development of an analytical structure to compare in vitro and in vivo radiosensitivity In the ... describe in vitro radiosensitivity (τ) and in vivo radiosensitivity (r) for each cell line and irradiation protocol Lines connect the same pairs of response points for each cell line as shown in figure...
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Báo cáo khoa học: "The alkylphospholipid, perifosine, radiosensitizes prostate cancer cells both in vitro and in vivo" docx
Ngày tải lên :
09/08/2014, 09:20
... radiationinduced cell death by the alkylphospholipid perifosine in CWR22RV1 prostate cancer both in vitro and in vivo In vitro, perifosine reduced cell viability and clonogenic survival, and enhanced ... adenocarcinoma cell line, CRW22RV1 [17] was cultured in RPMI 1640 containing 25 mM HEPES buffer, L-glutamine, 50 units/ml penicillin, 50 μg/ml streptomycin and 10% fetal bovine serum in a humidified incubator ... and phospho-Akt-S473 were observed in perifosine alone and the combination groups, indicating radiation combed with perifosine can increase the inhibitory effect of perifosine on Akt, resulting...
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