pylori interaction in both in vivo and in vitro
Tài liệu Báo cáo khoa học: The endogenous retinoid metabolite S-4-oxo-9-cis-13,14-dihydro-retinoic acid activates retinoic acid receptor signalling both in vitro and in vivo pdf
Ngày tải lên :
18/02/2014, 08:20
... all-trans-retinoic acid, the findings clearly demonstrate that S-4o9cDH-RA has the capacity to bind and activate nuclear retinoid receptors and regulate gene transcription both in vitro and in vivo Retinoids ... ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) and 1% nonessential amino acids (Gibco Invitrogen) Murine hepatoma-1 ... grown in low-glucose DMEM (Gibco Invitrogen) supplemented with 10% (v ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) and...
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Báo cáo hóa học: " Interaction Between Nano-Anatase TiO2 and Liver DNA from Mice In Vivo" pdf
Ngày tải lên :
22/06/2014, 00:20
... that there exists an interaction model of binding, i.e., a strong p-stacking interaction between Ti4? and DNA base pairs [19, 20] Ti4? can insert into DNA base pairs and bind to nucleotide Our ... 58, 7565 (1998) M.N Yoshioka, H Inoue, DNA binding of iron(II) mixed-ligand complexes containing 1, 10-phenanthroline and 4, 7-diphenyl-1, 10-phenanthroline J Inorg Biochem 77, 239–247 (1999) ... classical laddering cleavage in vivo Discussion In this study, the ICR mice were injected with various doses of nano-anatase TiO2 into abdominal cavity everyday for 14 days In the 50, 100 and 150 mg/kg...
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Báo cáo khoa học: "The alkylphospholipid, perifosine, radiosensitizes prostate cancer cells both in vitro and in vivo" docx
Ngày tải lên :
09/08/2014, 09:20
... radiationinduced cell death by the alkylphospholipid perifosine in CWR22RV1 prostate cancer both in vitro and in vivo In vitro, perifosine reduced cell viability and clonogenic survival, and enhanced ... adenocarcinoma cell line, CRW22RV1 [17] was cultured in RPMI 1640 containing 25 mM HEPES buffer, L-glutamine, 50 units/ml penicillin, 50 μg/ml streptomycin and 10% fetal bovine serum in a humidified incubator ... and phospho-Akt-S473 were observed in perifosine alone and the combination groups, indicating radiation combed with perifosine can increase the inhibitory effect of perifosine on Akt, resulting...
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Báo cáo y học: "Pre-clinical development as microbicide of zinc tetra-ascorbo-camphorate, a novel terpenoid derivative: Potent in vitro inhibitory activity against both R5- and X4-tropic HIV-1 strains without significant in vivo mucosal toxicity" docx
Ngày tải lên :
10/08/2014, 05:21
... with formalin and paraffin embedded by standard histological examination To assess gross tissue morphology, sections were stained with hematoxylin and eosin A vaginal irritation grading system ... C14 inhibited in vitro the infection of macrophages, DC and T cells that are the first cells targeted by HIV-1 in vivo Interestingly, at low concentrations, C14 inhibited more than 90% of both ... particles in vitro [25], caused lesions in the vaginal epithelium in vivo and increased the probability of being infected with HIV-1 [26] To assess whether biological activity of C14 causes inflammation...
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Báo cáo y học: "Expression of aromatase and estrogen receptor alpha in chondrosarcoma, but no beneficial effect of inhibiting estrogen signaling both in vitro and in vivo" ppt
Ngày tải lên :
13/08/2014, 15:21
... effect of inhibiting estrogen signaling both in vitro and in vivo Clinical Sarcoma Research 2011 1:5 Submit your next manuscript to BioMed Central and take full advantage of: • Convenient online submission ... addition, in spite of positive immunohistochemical staining for ESR1 protein in all in vitro cell cultures, addition of 17b-estradiol, 4-androstene-3,17-dione or drugs targeting the estrogen-signaling ... chondrosarcoma cells in vitro by measuring the effect of steroids and clinical drugs inhibiting estrogen-signaling We evaluated responsiveness of central chondrosarcoma cell lines and primary cultures...
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Teacher questioning strategies and Classroom interaction in Ly Thai To school
Ngày tải lên :
07/11/2012, 14:44
... There is no learning without teaching So as a tool in implementing teaching plans and achieving teaching goals, teacher talk plays a vital role in language learning Nunan (1991) points out that: ... situation in which interaction naturally blossoms and in which students can use for actual communication what they have been learning in a more formal fashion In this way, they are already engaging in ... teaching and learning is quite high in comparison with other schools in this area Part two: Development Chapter 1: Literature review 1 What is classroom interaction? Brown (1994) defines interaction...
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Tài liệu Báo cáo khoa học: Arsenite stimulated glucose transport in 3T3-L1 adipocytes involves both Glut4 translocation and p38 MAPK activity docx
Ngày tải lên :
21/02/2014, 00:20
... identified using antibodies against the IR b-chain and integrin-b1 (data not shown) As can be seen in Fig 2A, when probing the fractions with an antibody against GLUT4, both insulinand arsenite-treatment ... steps in both arsenite- and insulin-induced glucose uptake: activation of p38 MAPK and tyrosine-phosphorylation of c-Cbl In contrast to insulin, arsenite does not activate PI-3¢ kinase (and consequently ... Chen, D., Fucini, R.V., Olson, A.L., Hemmings, B.A & Pessin, J.E (1999) Osmotic shock inhibits insulin signaling by maintaining akt protein kinase B in an inactive dephosphorylated state Mol Cellular...
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Báo cáo khoa học: Definition of the residues required for the interaction between glycine-extended gastrin and transferrin in vitro pptx
Ngày tải lên :
07/03/2014, 02:20
... for the binding of gastrin, and of the regions in gastrin required for the interaction with transferrin, is obviously essential to a full understanding of the interaction The independent involvement ... first provided in a search for gastrinbinding proteins in porcine gastric mucosa [14] An interaction between Gamide and transferrin was identified by covalent crosslinking assays [14], and subsequently ... as a ligand for one ferric ion, and Glu8 and Glu9 bind a second ferric ion, in both Ggly [12] and Gamide [13] The interaction between Ggly and transferrin Although both Ggly and Gamide bind iron,...
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Báo cáo khoa học: Interaction between catalytically inactive calpain and calpastatin Evidence for its occurrence in stimulated cells docx
Ngày tải lên :
07/03/2014, 12:20
... Calpain–calpastatin interaction in stimulated cells M Averna et al Fig Effect of calpastatin on the binding of mAb 56.3 to immobilized calpain Immobilized calpain was exposed to increasing concentrations ... indicating that calpain had undergone a transition from the low to the high affinity mAb-binding form Interestingly, these results obtained in vivo were almost superimposable on those obtained in vitro ... recombinant rat brain calpastatin RNCAST104 in the presence of 10 mM EDTA (h) or lM Ca2+ (d) The binding of mAb 56.3 to immobilized calpain was measured as described in Figs and The data obtained in...
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Báo cáo khóa học: Surface nucleolin participates in both the binding and endocytosis of lactoferrin in target cells potx
Ngày tải lên :
07/03/2014, 15:20
... suggesting the presence of nucleolin-binding patterns in both lobes of hLf and, more particularly, in the N2 domain Furthermore, possible nonspecific ionic interactions between Lf and nucleolin were ... nucleolin and its fate following binding with Lf were investigated by confocal microscopy using the biotin-labeled hLf (hLf-biotin) and polyclonal antibodies against the C-terminal part of nucleolin ... binding site in nucleolin, i.e the C-terminal tail containing nine RGG repeats The conservation of this nucleolin domain among various species [22] probably accounts for the binding of hLf and...
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Báo cáo khoa học: Two types of replication protein A in seed plants Characterization of their functions in vitro and in vivo ppt
Ngày tải lên :
07/03/2014, 21:20
... CM (1998) Interaction of human rad51 recombination protein with single-stranded DNA binding protein, RPA Nucleic Acids Res 26, 5388–5393 17 Stauffer ME & Chazin WJ (2004) Physical interaction ... protein–protein interaction assay) (Fig 3C–E) In the two-hybrid assay, the interaction between OsRPA70a and OsRPA32 yielded about 20% of the relative activity of the OsRPA32 and OsRPA14 interaction ... tag were obtained by in vitro translation in wheatgerm extract (Invitrotec) according to the manufacturer’s procedure OsRPA70a (or OsRPA70b) and OsRPA32, or OsRPA32 and OsRPA14 were incubated with...
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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
Ngày tải lên :
07/03/2014, 21:20
... shown) In conclusion, we have shown using both in vitro and in vivo methods, that PSI-G adopts a topology in the thylakoid membrane with the loop facing the stroma and its N- and C-termini facing ... promoter and terminator, and into vectors for in vitro transcription and translation An Arabidopsis line (psag-1.4 [5], termed DG in this report), lacking PSI-G due to a transposon footprint in exon ... after 0, 30 and 60 digestion with trypsin, probed with antibodies directed against PSI-G, PSI-K (intrinsic membrane protein, stromal loop), PSI-O (intrinsic membrane protein, luminal loop) and PSI-D...
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Báo cáo khoa học: "CONTROL STRUCTURES AND THEORIES OF INTERACTION IN SPEECH" potx
Ngày tải lên :
08/03/2014, 18:20
... effort has gone into specifying global scheduling heuristics f o r maintaining an agenda of knowledge sourcc activation records m blackboard system~, and this has sometimes led to treating the control ... o m t h e perspective of interactive determinism, little has been said about the many other interactive channels which will be required for speech understanding and, in particular, whether, these ... t e r a c t m n b e t w e e n individual c o m p o n e n t s in a SUS INTERACTIVE DETERMINISM: A THEORY OF NON-SERIAL INTERACTION In this section, w e concentrate on the study of NSI b e t w...
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Báo cáo Y học: Inhibition of the SERCA Ca21 pumps by curcumin Curcumin putatively stabilizes the interaction between the nucleotide-binding and phosphorylation domains in the absence of ATP pot
Ngày tải lên :
08/03/2014, 23:20
... ATP into contact in the absence of ATP, curcumin is then able to bind to the ATPase (possibly at the hinge region) locking the two domains together and therefore precluding ATP binding (i.e inhibiting ... curcumin inhibition This scheme shows the potential effects of curcumin (I) on the interactions between the nucleotide binding domain (N) and the phosphorylation domain (P), to give competitive inhibition ... affected by curcumin FITC is known to label the ATPase on Lys515 within the ATP binding pocket of the nucleotide binding domain, also precluding ATP binding [15,43] Therefore if curcumin affects this...
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Báo cáo khoa học: PCSK9 is phosphorylated by a Golgi casein kinase-like kinase ex vivo and circulates as a phosphoprotein in humans doc
Ngày tải lên :
16/03/2014, 06:20
... et al endogenous inhibitor, a catalytic domain characteristic of serine proteases and a C-terminal Cys- and His-rich domain implicated in enzyme stability and protein– protein interaction [3] ... as insulin growth factor-binding proteins and osteopontin [25], and is unlike the complete modification reported for phosphoproteins secreted from mammary and salivary glands [25] Interestingly, ... parameters including: (a) stability and turnover by interfering with or promoting proteolysis [32,33], (b) activating or inactivating enzymes [35], (c) subcellular localization and transport [36,37], and...
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Báo cáo Y học: Amino acids 3–13 and amino acids in and flanking the 23FxxLF27 motif modulate the interaction between the N-terminal and ligand-binding domain of the androgen receptor pdf
Ngày tải lên :
17/03/2014, 10:20
... bovine serum (Life The ligand-dependent interaction between AR NTD and AR LBD, N/C interaction, was studied in yeast and mammalian in vivo protein interaction systems, and in Ó FEBS 2002 Interaction ... AR3)36 subdomain AR3)13 in N/C interaction and the role of individual amino acid residues in and flanking the 23 FQNLF27motif in AR16)36 in N/C interaction Yeast protein interaction assays indicated ... protein interaction assay are shown in Fig 8(B) Substitution of amino acids 23, 26 and 27 completely abolished interaction with GalDBD-AR.LBD and alanines at positions 24 and 25 increased the interaction...
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Báo cáo Y học: DNA supercoiling in Escherichia coli is under tight and subtle homeostatic control, involving gene-expression and metabolic regulation of both topoisomerase I and DNA gyrase docx
Ngày tải lên :
18/03/2014, 01:20
... previously [17] Supercoiling assay DNA supercoiling was assessed in terms of the linking number of intracellular plasmid pBR322 [18] DNA supercoiling was expressed as the active linking number, aLk, ... the difference in linking number of pBR322 in the respective sample and of pBR322 isolated from cells incubated for 30 with 0.1 mgámL)1 of coumermycin and 0.2 mgámL)1 rifampicin Topoisomerase ... containing the N-terminal part of topA was inserted This resulted in the plasmid pTOPA2A5TS, in which the pA1lacO-1 promoter and the lacIq1 gene are surrounded by a DNA fragment originating from...
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Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx
Ngày tải lên :
23/03/2014, 13:20
... CPS and O-chain LPS are composed of linear trisaccharide repeating units containing 3-linked 2-acetamido-2-deoxy-D-quinovose, 4-linked 3-[(N-acetyl-L-alanyl) amido]-3-deoxy-D-quinovose and 3-linked ... Interresidue NOEs were observed between H-1a and H-3c and between H-1b and H-3a, suggesting a linear structure a-c-b (Fig 2E,F) In addition, interresidue NOEs between H-1c and H-4b (Fig 2G) indicated ... (GalNAcAcontaining trisaccharide repeating unit) (Fig 4A, inset), based on this ratio about 40% of GalNAcA in both CPS and O-chain polysaccharides was present in the form of a primary amine When...
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Báo cáo khoa học: In vitro and in vivo self-cleavage of Streptococcus pneumoniae signal peptidase I pot
Ngày tải lên :
23/03/2014, 21:21
... the protein The self-cleavage of LexA protein is important in the SOS response in bacteria [21–23] In vitro self-cleavage was also observed in all investigated bacterial SPase I including enzymes ... results intrigued us to investigate the cleavage of S pneumoniae SPase I in vivo As shown in Fig 6C, Western blot analysis demostrated that the self-cleavage of SPase I is indeed occuring in vivo in ... the interaction between the enzyme and the cytoplasmic membrane In this study, we were interested in investigating the effect of phospholipid on the self-cleavage of S pneumoniae SPase I In the...
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Báo cáo khóa học: Determination of modulation of ligand properties of synthetic complex-type biantennary N-glycans by introduction of bisecting GlcNAc in silico, in vitro and in vivo pot
Ngày tải lên :
30/03/2014, 13:20
... nonspecific binding by protein–protein interactions, cells were incubated with 100 lg ligand-free carrier protein (BSA) per mL for 30 at °C before incubation with the biotinylated neoglycoprotein in Dulbecco’s ... assay, saturable and carbohydrate-dependent binding was measured, and the resulting Scatchard plots gave straight lines indicating the presence of a single class of binding sites in each case (not ... neoglycoproteins, we determined their binding to cells and to tissue sections as well as their biodistribution in mice after injection of radioiodinated material Binding to cells and organs In these...
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