and o glycosylation in e coli

... data were reweighted after selecting the model to obtain better estimates of kinetic parameters The dosage regimen of ceftriaxone was also determined based on the kinetic data The priming (D) and ... 5% Each sample was diluted to the extent that its zone of inhibition came in linear range (preferably in the range of the zone of inhibition of the reference concentration) In this experiment, ... regression The data gave best fit to the threecompartment model Akaike information criterion (AIC) and MAICE (minimum Akaike information criterion) values were applied to select the model The...

Ngày tải lên: 07/08/2014, 18:21

... was somewhat insoluble, since we detected high concentration of this protein in the pellet fractions after h induction (Figure 2, panel E) When comparing gene expression in the different E coli ... protein were loaded on to the column Lane 1, protein not retained by the asialofetuin-sepharose column Lane column washes Lane through 5, fractions retained on the column Immunodetection was performed ... SDS-PAGE and Coomassie blue staining Purification of recombinant proteins After recombinant protein induction, cellular pellets were resuspended in lysis buffer We tested four different concentrations...

Ngày tải lên: 12/08/2014, 04:21

... used in expression experiments was sufficient to express and produce the optimum amount of cHSPA6 in E coli In addition, the lower concentration of IPTG had no adverse effect on the growth rate ... protein Therefore, for further optimization experiments a 40-fold lower IPTG concentration than the normal was used Figure SDS–PAGE analysis of effect of inducer concentration on the overexpression ... diagram of the hexa-histidine tagged cHSPA6 fusion protein 2.2 2.2.1 Optimization of cHSPA6 overexpression in E Coli Effect of temperature on the overexpression of cHSPA6 Figure showed that 37...

Ngày tải lên: 24/10/2016, 14:24

... region of clotting factor IX is a signal for a vitamin K dependent carboxylase: evidence from protein engineering of amino acid -4", Nucleic Acids Res, 15(22), pp 9505-9513 34 Green P M., Bentley ... polymerase to direct selective high-level expression of cloned genes", J Mol Biol, 189(1), pp 113-130 57 Sunnerhagen M S., Persson E. , Dahlqvist I., Drakenberg T., Stenflo J., Mayhew M., Robin ... Den Berg M., Poort S R., Bertina R M (1997), "Modification of the N-terminus of human factor IX by defective propeptide cleavage or acetylation results in a destabilized calcium-induced conformation:...

Ngày tải lên: 10/02/2014, 20:39

... Nekemte town and within 10 km were randomly selected to be included in the study These eleven kebeles then, divided in to several “Gotts” and representative “Gotts” were selected based on their ... were also used to assess the association between people involved in the discussions and topics discussed Young people who were educated to high school and tertiary level were more likely to communicate ... communication about SRH with the young people by gender, Nekemte, West Ethiopia, 2012 People involved in the communication Proportion of people involved by respondents’ gender Male Yes Female No 36(10.3%)...

Ngày tải lên: 22/03/2014, 12:20

... ereveS )nwohs ton atad( seussit lasrub eht fo selcillof eht fo xetroc dna alludem eht ni htob noitelped dna sisorcen diohpmyl )5 erocs( ereves dna )4 erocs( ereves ot etaredom fo ecnedive dewohs ... 01 01 E iloc E iloc E iloc E iloc E iloc E noissucsiD iloc E egnellahc VDBIvh eht devivrus snekcihc morf devresbo erew setycohpmyl fo noitelped )3+ erocs( etaredom dna )2+ erocs( etaredom ot dlim ... nwohs ylsuoiverp neeb sah ti ,eromrehtruF metsys enummi nekcihc eht ot detneserp dna demrof ton erew ,sepotipe lanoitamrofnoc yllaicepse sepotipe laicurc emos ,detalysocylg ylroop si ni desserpxe...

Ngày tải lên: 07/08/2014, 18:21

... become ‘free’ (plectonemic) in the absence of chromatin proteins From a DNA topology perspective, the plectonemic supercoils contain more potential energy, in terms of driving superhelicaldependent ... replication origin and terminus are marked in bold The outer circle (1) shows the change in expression of genes in response to supercoiling (log p values), where more negative values correspond to genes ... position preference’ measure is plotted in circle As expected, regions of low position preference tend to correspond to the regions with highly expressed genes found by Jeong et al [1] However,...

Ngày tải lên: 14/08/2014, 14:21

... in the circles represent regions of homology with the other genomes Each circle represents a different genome, with the outer five circles representing nonreduced genomes, and the inner three circles ... indicated outside the circles Coding sequences of the reference genome (E coli k-12 strain MG1655) are indicated as blue and red blocks, corresponding to genes orientated clockwise or counterclockwise ... as the reference here The locations of all the deletions engineered by Posfai et al [5] are indicated outside the circles The location of each gene from the E coli MG1655 genome is used to visualize...

Ngày tải lên: 14/08/2014, 17:22

... samples were collected biweekly during four months of intervention in 2009 (control) and 2010 (intervention and 2) E. coli contamination level of household drinking water from the intervention group ... containers (intervention group) We selected another 50 households which stored drinking water after boiling in 20 L plastic narrow-necked containers (intervention group) Treated drinking water ... (P

Ngày tải lên: 11/08/2015, 12:20

... Dideoxynucleoside triphosphate Dideoxynucleoside triphosphate DNA Deoxyribonucleic acid Axít Deoxyribonucleic dNTP Deoxynucleoside triphosphate Deoxynucleoside triphosphate E coli Escherichia coli ... coli Escherichia coli EDTA Ethylenediaminetetraacetic acid Ethylenediaminetetraacetic acid EtBr Ethidium Bromide Ethidium Bromide EtOH Ethanol Ethanol IPTG Isopropyl-thio-β-D-galactoside Isopropyl-thio-β-D-galactoside ... acid ribosome RNA Reverse Transcriptase- Polymerase Kỹ thuật PCR enzyme phiên mã Chain Reaction ngược SDS Sodium Dodecyl Sulphate Sodium Dodecyl Sulphate SDSPAGE Sodium Dodecyl Sulphate polyacrylamide...

Ngày tải lên: 04/11/2015, 05:17

... on sepsis, macrophages were depleted in vivo using Clodronate-Liposome injections three and one day before E coli challenge Macrophage depletion prevented the L.s.-mediated protective effect on ... the duration of six hours, mice were monitored for the development of hypothermia After six hours mice were euthanized and levels of pro-inflammatory cytokines and chemokines were determined in ... when compared to L.s.-infected mice that received PBScontaining liposomes Of note, injection of Clodronate liposomes alone did not induce detectable amounts of pro-inflammatory cytokines in the...

Ngày tải lên: 26/11/2015, 10:00

... tocopherol chromanolring, thus to produce tocopherol The study shows that by over-expressing the P frutescens γ-PfTMT gene in E coli, the tocopherol composition increase in α-tocopherol content ... insights into these processes [124], and investigation of the genome sequence of T reesei showed that this industrial workhorse possesses the smallest number genes within Sordariomycetes encoding the ... these names have been innovated long before molecular methods for species classification were obtainable They are likely to have get obsolete in the meanwhile Nowadays, the International Subcommission...

Ngày tải lên: 22/05/2016, 11:28

... degradation of macromolecules and targeting of lysosomal acid hydrolases and cholesterol to the lysosome and processing of various secretory proteins including prohormones in the trans-Golgi network ... conclude that the sorting and segregation of cathepsin E from other secretory proteins is independent of mannose 6-phosphate mechanisms Experimental procedures Materials [35S]Methionine ⁄ cysteine and ... mannose 6-phosphate receptors and its delivery to the prelysosomal compartment To determine whether the oligosaccharides of cathepsin E are phosphorylated, the transfected cells were labeled with...

Ngày tải lên: 16/03/2014, 14:20

... base linkage Comparison of the deduced amino acid sequence of oraE to those of known PLPdependent aminomutases reveals the presence of a conserved PLP-binding site, a lysine residue at position ... speed of 180 r.p.m It is worth noting that, alone, OraS protein can be expressed in a soluble form However, the OraE and OraS proteins were coexpressed in an Fig The over-expression of oraS and oraE ... nucleotide sequence of the ora genes in order to facilitate the amplication by PCR An NcoI site was introduced into the start of the oraS gene and a BamHI site into the end of the oraE gene Genomic...

Ngày tải lên: 30/03/2014, 15:20

... These observations indicate that after the endotoxin challenge, Ig concentrations in efferent lymph were mainly influenced by the contents of the afferent lymph, flowing into the node and to ... reports, clearly confirming an inflammatory response It can be questioned whether the observed inflammatory responses really were due to the endotoxin infusion and Page of 10 (page number not ... there was no indication of an inflammatory reaction being present in the udder tissue before the endotoxin was infused and it is likely that the observed inflammatory response was due to the endotoxin...

Ngày tải lên: 12/08/2014, 18:22

... increases were also calculated for quinolones and for third-generation and fourth-generation cephalosporin use Consumption of the whole class of cephalosporins and penicillins, however, decreased ... analyse continuously nosocomial infections and resistant pathogens [15] These routine data were reported to the National Reference Centre of the Surveillance of Nosocomial Infections Ethical approval ... resistance proportions between and 15% in 2008 (b) Carbapenem use The pooled mean (solid line) and the median (dotted line) run almost parallel to one another In most ICUs the increase in carbapenem...

Ngày tải lên: 13/08/2014, 20:22

... Adherence Enteropathogenic Escherichia coli Brain-heart infusion Cng s Colonial Forming Unit Edema disease Edema disease pathogenic Enterohaemorrhagic Escherichia coli Eosin Methylene Blue Agar ... Agar Enteropathogenic Escherichia coli Enterotoxigenic Escherichia coli Heamolysin Heamolysin Khỏng nguyờn Heat-Labile enterotoxin Nh xut bn Polymerase Chain Reaction Shiga-like toxin Shiga-like ... toxin Shiga-like toxin Heat-Stable Enterotoxin (a,b) Heat-Stable1 Shiga toxin 2e Trang Triple Surgar Iron Th trng Ultraviolet Voges Pros Kauer Verotoxin 2e Verotoxigenic Escherichia coli Clostridium...

Ngày tải lên: 06/11/2012, 11:22

... perfringens E coli : Escherichia coli EMB : Eosin Methylene Blue Agar EPEC : Enteropathogenic E coli ETEC : Eneterotoxigenic E coli ETEE : Enterotoxinic E coli F : Fimbriae GTP : Guanosin 5-Triphosphate ... E coli phân loại theo yếu tố gây bệnh mà chúng có khả sản sinh như: Enterotoxigenic E coli ký hiệu ETEC, Enterotoxinic E. coli ký hiệu ETEEC, Enterotopathogenic E coli ký hiệu EPEC, Verotoxigenic ... Deboxy nucleic AEEC : Adhereneia Enteropathogenic E coli AMP : Adenosine Monophosphate ATP : Adenosin Triphosphate BHI : Brai-heart infusion Colv : Colicin V C perfrigens : Clostridium perfringens...

Ngày tải lên: 12/11/2012, 11:59

... Stx 2e TSI UV VK VP VT 2e VTEC : Adhenicia Enterropathogenic Escherichia coli : Brain-heart infusion : Colinial Forming Unit : Edema disease : Edema disease pathogenic : Entero heamorrhagic : Eosin ... Eosin Methylene Blue Agar : Enteropathogenic Escherichia coli : Enterotoxigenic Escherichia coli : Heamolysin : Kháng nguyên : Heat-Labile enterotoxin : Nhà xuất : Page : Polymerase Chain Reaction ... cầm, ngƣời ta v o yếu tố gây bệnh để chia chúng v o nhóm:Enterotoxigenic(ETEC),Enteropathogenic(EPEC),Enteroinvasive(EIEC) ,Enterohemorrhagic(EHEC) Attaching and Effacing E. coli( AEEC) 1.2.6.1 Các...

Ngày tải lên: 12/11/2012, 14:43