an flc is characterized by a set

... recruited and followed up the early arthritis patients, analyzed and interpreted the data, and drafted the manuscript FF performed the statistical analysis and was involved in drafting the manuscript ... American Rheumatism Association (ARA) criteria for RA had a cytokine profile characterized by a range of T cell, stromal cell and macrophage related cytokines that was not present in long-standing ... represent patients with (panel b) all other early inflammatory arthritis and (panel d) established RA In other studies comparing early RA (defined as a symptom duration of

Ngày tải lên: 09/08/2014, 06:22

... 365–377 Biswas, G., Adebanjo, O .A. , Freedman, B.D., Anandatheerthavarada, H.K., Vijayasarathy, C., Zaidi, M., Kotlikoff, M & Avadhani, N.G (1999) Retrograde Ca2+ signaling in C2C12 skeletal myocytes ... d-aminolevulinate synthase (ALAS) and b-F1-ATPase genes [29,34] In both constructs there was a substantial Fig Functional analysis of the D melanogaster a- F1-ATPase promoter Scheme of the a- F1-ATPase ... differentiated, both structurally and functionally, with highly developed cristae full of ATP synthase complexes For this reason, ATP synthase and in particular the a- F1-ATPase and b-F1-ATPase catalytic...

Ngày tải lên: 07/03/2014, 16:20

... (Burlington, Canada) and the goat anti-(C-terminal PCSK9) IgG used for immunoblotting from Imgenex (San Diego, CA, USA) Secondary anti-mouse and anti- (rabbit HRP) IgG were from Amersham (Piscataway, NJ, ... in media containing 32P-orthophosphate, immunoprecipitated PCSK9 from cells and media, and analyzed samples by SDS ⁄ PAGE fractionation followed by phosphorimaging (Fig 5) Lanes and and and represent ... intracellularly Radiolabeling and analyses were also conducted for untransfected and transfected Huh7 cells PCSK9 circulates as a phosphoprotein in humans hPCSK9(WT) (lanes and 7, respectively) Again...

Ngày tải lên: 16/03/2014, 06:20

... 45AAA 45EEE 104AYGEAEA 122AA 145AA 151AA 161AAA 175AAA 192ASAAA 198AAAAA 206AAPA 24AAAA 34AAAA 54F 70AAAMA 76APAKADAA 112AASRAVAAAVA 133H 141AASAEECKAA 164AAHARPA 179AA 183H 196AA 207AA 214AAGA ... DNA sequence of the primer AAC ATA TGG ATC AGG AAG CTC TAG ATT AC AAC ATA TGG CAC GAG AAA AGT CTA CCT TC AAC ATA TGA TCA CGC ACT ACC GCC C TCC ATA TGC TAG AAG GGG TTG CCT GGC GGA TCC CTA GAA ... GGG GAT CCC TGG CCC CAG AGC GG TTG GAT CCT TAT GTA CTA GCA AGC AGC C TTG GAT CCT TAT TGC CGA GTA CTA TGT TC TTG GAT CCT TAG GGA GAA GAA GGT GAT G AAA GAT CTT TAT TCC TTT TCT TCC TTG CG temperature...

Ngày tải lên: 23/03/2014, 12:20

... are distinguished by different shades of blue The image was generated using PYMOL dues in the bacterial and mammalian templates P falciparum Glu295 aligns with an Asp in mammals (human arginase ... in Plasmodium falciparum proteins are primarily nucleic acid level adaptations Mol Biochem Parasitol 128, 21–32 58 Yuvaniyama J, Chitnumsub P, Kamchonwongpaisan S, Vanichtanankul J, Sirawaraporn ... sickness, Chagas’ disease, and leishmaniasis Amino Acids 33, 359–366 12 Wallace HM (2007) Targeting polyamine metabolism: a viable therapeutic ⁄ preventative solution for cancer? Expert Opin Pharmacother...

Ngày tải lên: 29/03/2014, 23:20

... GCTGAAGACCGCGGAGGTAC GAGGTACCCAGAACTGCCCTG GATAGTCAGTGGGGGAAACCTCAG CAGCAAAAGAGGGCTGTGTGGTG TGGCCGCCACGAACATCCCATC GTTGCCCAGGTTGGAGTGCAG CTCGCGGGCTCGGCAGTGGGAG AGTCTATTCTCGAGCACCTGGGACTACAGG AGTCTATTCTCGAGCCCAAAGCGCTGAGATTACAG ... AGTCTATTCTCGAGCCCAAAGCGCTGAGATTACAG AGTCTATTCTCGAGATGCTTCTGGAGTAGGAGGCA AGTCTATTCTCGAGGTGTGGAGGAGGCAGGGAGAC AGTCTATTCTCGAGGAGGCGCTCTGCAGTGCCTC AGTCTATTCTCGAGAAGCAGGACGTTCCCACGCTG AGTCTATTCTCGAGGGGGCGGGCGCCCGCACTCAG ... Reuter I, Hermjakob H, Kel AE, Kel OV, Ignatieva EV, Ananko EA, Podkolodnaya OA, Kolpakov FA et al (1998) Databases on transcriptional regulation: TRANSFAC, TRRD and COMPEL Nucleic Acids Res 26,...

Ngày tải lên: 30/03/2014, 03:20

... Gly-Arg-pNA Ala-Ala-Pro-pNA Ala-Phe-Pro-pNA Ala-Ala-Ala-pNA Pro-Leu-Gly-pNA Ala-Ala-Phe-pNA Suc-Ala-Ala-Phe-pNA Val-Leu-Lys-pNA Cbz-Pro-Phe-Arg-pNA Ala-Ala-Val-Ala-pNA Ala-Ala-Pro-Leu-pNA Suc-Ala-Ala-Pro-Phe-pNA ... hydrolyse Ala-Ala-pNA and Ala-pNA (or other chromogenic amino acids) at reasonable rates clearly indicates exclusive cleavage of the anilide bond of Ala-Ala-Val-Ala-pNA Our results also provide ... mixtures of SM-TAP and a TGase fragment were incubated for 30 and separated by SDS/PAGE N-Terminal sequence analysis of TGase clearly showed that SM-TAP removes Arg-Ala-Pro and Phe-Arg-Ala-Pro from...

Ngày tải lên: 31/03/2014, 07:20

... plasma samples was approximately ng/mL (100 pg/injection) Statistical analysis Data were analyzed with a two-way analysis of variance (ANOVA) with group and time as the two factors Statistical ... Takeda N, Hasegawa S, Morita M, Horii A, Uno A, Yamatodani A, Matsunaga T: Neuropharmacological mechanisms of emesis I Effects of antiemetic drugs on motion- and apomorphineinduced pica in rats ... called pica behavior Pica in rats is thus analogous to nausea and vomiting in humans and other species [11,13] Pica in rats is mediated by mechanisms and receptors involving serotonin and dopamine,...

Ngày tải lên: 10/08/2014, 05:21

... mediators Ann N Y Acad Sci 1989, 557:310-5; discussion 315-6 Shimada H, Hasegawa N, Koh H, Tasaka S, Shimizu M, Yamada W, Nishimura T, Amakawa K, Kohno M, Sawafuji M, Nakamura K, Fujishima S, Yamaguchi ... [GenBank: AF185285], sense: 5'-GCT TAC ACC ACC TCT CAA ACT TGA T-3', antisense: 5'-ATT ACC TCT TAG AGT CAG TTC ATG G-3'; β-actin [GenBank: X03672], sense: 5'-ATG GAT GAC GAT ATC GCT-3', antisense: ... anti-mouse vascular cell adhesion molecule-1 (VCAM-1) (Santa Cruz Biotechnology, Inc Santa Cruz, CA), rabbit anti-signal transducer and activator of transcription 1, total (STAT1), and antiphospho-STAT1...

Ngày tải lên: 11/08/2014, 08:21

... Dial Transplant 2008, 23:3737-3743 Haase M, Bellomo R, Devarajan P, Schlattmann P, Haase-Fielitz A: Accuracy of neutrophil gelatinase-associated lipocalin (NGAL) in diagnosis and prognosis in acute ... clinical settings [3] Most of the studies evaluating NGAL as a predictor of AKI used a research-based ELISA assay The availability of a standardized clinical platform for NGAL measurement would make ... kidney injury are associated with hospital mortality in critically ill patients: a cohort analysis Crit Care 2006, 10:R73 Devarajan P: Neutrophil gelatinase-associated lipocalin an emerging troponin...

Ngày tải lên: 13/08/2014, 20:22

... TMPRSS13 mRNA is expressed in a variety of human adult tissues, and predominantly in lung, placenta, pancreas and prostate [12] HAI-1 mRNA is also highly expressed in placenta, pancreas and prostate ... (Santa Cruz, CA, USA); and horseradish peroxidase-conjugated anti-rabbit and anti-mouse IgG from GE Healthcare UK (Buckinghamshire, UK) The anti-c-Myc IgG was prepared as follows An anti-c-Myc IgG ... 5¢-GGATTTTCTGAATCGCACCT-3¢ for TMPRSS13 (34 cycles), and 5¢-ATGGAGGCTGCTTGG GCAACA-3¢ and 5¢-ACAGGCAGCCTCGTCGGAGG-3¢ for HAI-1 (26 cycles) The GAPDH-specific primer set, 5¢-AGGTGAAGGTCGGAGTCAAC-3¢ and...

Ngày tải lên: 15/02/2014, 01:20

... AGTAAGGAAAATAAGCTTGGGCATGATCCC GCTCACTGCCTAAGCTTTGTAGCTAATAAAG CTTTATTAGCTACAAAGCTTAGGCAGTGAGC CTTTGTTATTTATTATGCATTCCTATGGTGAGTT AACTCACCATAGGAATGCATAATAAATAACAAAG CTTTGTTAAAGCTTATGCATTCCTATGGTGAGTT AACTCACCATAGGAATGCATAAGCTTTAACAAAG ... SJS170 ALS030 SJS209 SJS275 SJS276 CGGAAGATCTAACTAAGCGTGCTGCTTC TACGAGATCTGTTGTTTGGAAGCAGGTT CGGAAGATCTGGGATCATGCCCATTTAG TACGAGATCTTAGCTACATTAAATAGGC GGGATCATGCCCAAGCTTATTTTCCTTACT AGTAAGGAAAATAAGCTTGGGCATGATCCC ... AACTCACCATAGGAATGCATAAGCTTTAACAAAG CCTCTTACACTTGCTTTTGAC GCAAAGGTGCCTTTGAGGTTG GACCCCTTCATTGACCTCAACTA CTTGATTTTGGAGGGATCTC TTAGCTACATTAAATAGGCAG GtaatacgactcactataGGGATCATGCCCATTTAG Forward (nt 1281–1298...

Ngày tải lên: 16/02/2014, 09:20

... was analysed by electrophoresis on 4% polyacylamide (nondenaturing) gels and visualized in a Phosphorimager Lanes as marked The heparin-resistant complex on tRNA1Gly -1 (left) is marked by an ... Regulation of pol III transcription A Parthasarthy and K P Gopinathan and making them unavailable for transcription The ‘TATATAA’ sequence present in the flanking regions Gly of tRNA1 -6,7 was responsible ... FEBS A Parthasarthy and K P Gopinathan completely abolished when the TATATAA sequence was mutated to GATATCA (lanes 3, 4) These results were also consistent with the observation that TFIIIB alone...

Ngày tải lên: 20/02/2014, 02:21

... d(5¢-AACAACGCAGCTGGGCTCTG GAACCAT), ECF -A1 41Q d(5¢-TCAACCTCTAACCAG GCTACTCCGCTG) ECM-G77Q d(5¢-AACAACGCTGG CCAGCACGCTAACCAC) and ECM-Q14 6A d(5¢-TCT ACTGCTAACGCGGATTCTCCGCTG) following the manufacturer’s ... Further analysis of these and other mutant SODs is currently underway ACKNOWLEDGEMENTS We are indebted to G Peplow, F Yamakura and T Matsumoto for the analyses of iron and manganese in protein samples ... human manganese superoxide dismutase: effects on structure, stability, and catalysis Biochemistry 39, 7131– 7137 31 Hiraoka, B.Y., Yamakura, F., Sugio, S & Nakayama, K (2000) A change of the metal-specific...

Ngày tải lên: 21/02/2014, 01:21

... following antibodies were used: anti-ADAM10, a polyclonal rabbit antibody against endogenous ADAM10 and anti-TACE, and a polyclonal rabbit antibody against endogenous TACE (Chemikon International, ... substrate CDPstar (Tropix) Emitted light was densitometrically analyzed by using a digital camera and the software AIDA 2.0 (Raytest, Straubenhardt, Germany) Isolation and detection of APPsa by ... (5¢-GTGGGCCGGAAAGTGAGCCA-3¢) and Fur2_rev (5¢-CCCTTGTAGGAGATGAGGCC-3¢) The resulting 1058 bp amplificate was isolated, subcloned in pUC57 (MBI Fermentas) and sequenced Western blot analysis of TACE and ADAM10...

Ngày tải lên: 08/03/2014, 02:20

... violaxanthin to antheraxanthin than on conversion of antheraxanthin to zeaxanthin (Table 1) At low monogalactosyldiacylglycerol concentration (5 mol%), probability of violaxanthin to antheraxanthin ... which all molecules of violaxanthin are converted to antheraxanthin and all molecules of antheraxanthin are converted to zeaxanthin at a given temperature at saturating amount of VDE and at the ... of violaxanthin to antheraxanthin is much slower and more sensitive to temperature than transition from the antheraxanthin to zeaxanthin On the basis of our results and literature data [15,22],...

Ngày tải lên: 08/03/2014, 10:20

... Comparisons of tryptophan hydroxylase from a malignant murine mast cell tumor and rat mesencephalic tegmentum Arch Biochem Biophys 199, 355–361 13 Hasegawa, H., Yanagisawa, M., Inoue, F., Yanaihara, ... phenylalanine hydroxylase (and two other proteins, molecular masses of 95 kDa and 60 kDa that contaminated the preparation) was clearly phosphorylated (Fig 3A) When an effort was made to enhance ... SDS/PAGE for autoradiography as described in Materials and methods The autoradiogram (left panel, 32P) represents treatment with vehicle (lane 1), 0.5 lM okadaic acid (lane 2), and okadaic acid...

Ngày tải lên: 08/03/2014, 16:20

... CGGGGGAGCGGGCGATGACCT GCAGATGCGCGTGCCAGACC CGGCGCCAGTAGCCGACGAAG CGGGTGGCCGCCAAACTCG AGGAAGCGCGGTCAAGGGAGTCTC CGCAAGGCGCTGGCCGAGTTCA TGTGCAGCAGCGGGACGTAGTAGG GGAATTCCGCGCGCGGGTCTGGCTTCA RT-PCR RT-PCR ... the mutant with aac(3)IV fragment (935 bp) as probe, as expected Hybridization and PCR analysis results indicate that the desA gene has been deleted and replaced by the apramycin resistance gene ... encoded by desA and its possible involvement in desferrioxamine biosynthesis, two apramycin-resistant and kanamycin-sensitive transformants were isolated among S coelicolor transformants with...

Ngày tải lên: 16/03/2014, 11:20

... arrow marked ‘c std’ Lane 2, lysate sample alone Note that a separation between c-3FLAG standard and e-CTF-3FLAG was achieved in lane An uncharacterized anti-FLAG immunoreactive protein migrating ... indistinguishable from that of e-3FLAG standard (Fig 3B, lane 1) but a slightly faster mobility than the c-3FLAG standard (Fig 3C, lane 1) Using C101-3FLAG, c-3FLAG and e-3FLAG standards as molecular ... e-CTF-3FLAG band was greater in lane spiked with e-3FLAG standard (C) A similar experiment to that in (B) was performed except that lane is a sample spiked with c-3FLAG standard indicated by the arrow...

Ngày tải lên: 16/03/2014, 23:20

... 419–426 Kenji Yasuda, Fumiaki Sugaya, Toshiyuki Takezawa, Seiichi Yamamoto and Masuzo Yanagida 2003 Applications of Automatic Evaluation Methods to Measuring a Capability of Speech Translation System ... 14th International Conference on Computational Linguistics: 433–439 Simone Teufel and Hans van Halteren 2004 Evaluating Information Content by Factoid Analysis: Human Annotation and Stability Proceedings ... ROUGE-2 or is used as a similarity measure, and a regression analysis is used for combining manual method References Robert L Donaway, Kevin W Drummey and Laura A Mather 2000 A Comparison of Rankings...

Ngày tải lên: 17/03/2014, 04:20