amp 948 endotoxins of bacillus thuringiensis

... annotation of a novel large genome bacteriophage named Bacillus thuringiensis phage 0305φ8-36 [1,2] Phage 0305φ8-36 was isolated from soil while targeting the isolation of large, unusual phages of unsampled ... http://www.virologyj.com/content/4/1/97 Figure the genome of 0305φ8-36 showing distribution of features Map of Map of the genome of 0305φ8-36 showing distribution of features The features are from ref [1] The ... spectrometric analysis of highly diverse, atypical Bacillus thuringiensis phage 0305phi8-36 Virology 2007, 368:405-421 Thomas JA, Hardies SC, Serwer P: The complete genomic sequence of Bacillus thuringiensis...

Ngày tải lên: 18/06/2014, 18:20

... annotation of a novel large genome bacteriophage named Bacillus thuringiensis phage 0305φ8-36 [1,2] Phage 0305φ8-36 was isolated from soil while targeting the isolation of large, unusual phages of unsampled ... http://www.virologyj.com/content/4/1/97 Figure the genome of 0305φ8-36 showing distribution of features Map of Map of the genome of 0305φ8-36 showing distribution of features The features are from ref [1] The ... spectrometric analysis of highly diverse, atypical Bacillus thuringiensis phage 0305phi8-36 Virology 2007, 368:405-421 Thomas JA, Hardies SC, Serwer P: The complete genomic sequence of Bacillus thuringiensis...

Ngày tải lên: 20/06/2014, 01:20

... H.R Whiteley, Insecticidal crystal proteis of ¨ Bacillus thuringiensis, Microbial Rev 53 (1989) 242 – 255 K.A Barton, H.R Whiteley, N.S Yang, Bacillus thuringiensis l-endotoxin expressed in transgenic ... of protein for people living in these areas The development of YSB-resistant varieties of DWR will help farmers in flood-prone ecosystems significantly The crystal protein or l-endotoxin from Bacillus ... these areas The yield of these cultivars is generally low (1 – t − 1) and very often, reduced further by insect attack Stem borers are important pests of rice because symptoms of injury can be found...

Ngày tải lên: 23/03/2014, 22:20

... of action of Cry toxin for M sexta is that binding of monomeric Cry1Ab toxin to BT-R1 promotes additional proteolytic cleavage in the N-terminal end of the toxin, facilitating the formation of ... mechanism of Cry1A toxins with BT-R1 and APN receptor molecules [30,31] However, a different mechanism of action of Cry toxins was recently proposed, based on a study of the effect of Cry1Ab ... Ministry of Education, Culture, Sports, Science, and Technology of Japan References Aronson AI, Beckman W & Dunn P (1986) Bacillus thuringiensis and related insect pathogens Microbiol Rev 50, 1–24 Hofmann...

Ngày tải lên: 24/03/2014, 00:21

... analysis of their binding activity to the receptor to investigate the role of mutated amino acid residues; (b) the analysis of the influence of the modification of the cysteine residues of mutants ... Role of receptors in Bacillus thuringiensis crystal toxin activity Microbiol Mol Biol Rev 71, 255–281 Aronson AI, Wu D & Zhang C (1995) Mutagenesis of specificity and toxicity regions of a Bacillus ... Knowles BH & Ellar DJ (1987) Colloid-osmotic lysis is a general feature of the mechanisms of action of Bacillus thuringiensis d -endotoxins with different insect specificities Biochim Biophys Acta 924,...

Ngày tải lên: 29/03/2014, 22:21

... Cytocidal Actions of Parasporin-2, an Anti-tumor Crystal Toxin from Bacillus thuringiensis J biol chem 2006, 281:26350-26360 16 Chan KK: Effects and Mechanism of action of Bacillus thuringiensis ... SS, Soberón M: Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control Toxicon 2007, 49:423-435 12 Thomas WE, Ellar DJJ: Bacillus thuringiensis var israelensis ... immunostaining pattern of these Krishnan et al Journal of Biomedical Science 2010, 17:86 http://www.jbiomedsci.com/content/17/1/86 Page of 11 Figure Confirmation of Bt18 binding to GAPDH via immunofluorescence...

Ngày tải lên: 10/08/2014, 05:21

... từ Bacillus thuringiensis var.israelensis cần phải đợc nghiên cứu thêm Phần I:Tổng quan Bacillus thuringiensis 1.1 Tóm tắt lịch sử nghiên cứu ứng dụng Bacillus thuringiensis Lịch sử Bacillus thuringiensis ... cánh cứng, hai cánh số loài phụ Bacillus thuringiensis var.berliner, Bacillus thuringiensis var.thompsoni Khác với loài Bacillus thuringiensis, loài phụ Bacillus thuringiensis var.israelensis đợc ... phân loại loài phụ Bacillus thuringiensis var.israelensis Bacillus thuringiensis var.israelensis loài phụ loài Bacillus thuringiensis De Bajac ngời phân loại loài phụ Bacillus thuringiensis var.israelensis...

Ngày tải lên: 10/12/2012, 10:45

... từ Bacillus thuringiensis var.israelensis cần phải đợc nghiên cứu thêm Phần I:Tổng quan Bacillus thuringiensis 1.1 Tóm tắt lịch sử nghiên cứu ứng dụng Bacillus thuringiensis Lịch sử Bacillus thuringiensis ... cánh cứng, hai cánh số loài phụ Bacillus thuringiensis var.berliner, Bacillus thuringiensis var.thompsoni Khác với loài Bacillus thuringiensis, loài phụ Bacillus thuringiensis var.israelensis đợc ... phân loại loài phụ Bacillus thuringiensis var.israelensis Bacillus thuringiensis var.israelensis loài phụ loài Bacillus thuringiensis De Bajac ngời phân loại loài phụ Bacillus thuringiensis var.israelensis...

Ngày tải lên: 22/04/2013, 15:19

... từ Bacillus thuringiensis var.israelensis cần phải đợc nghiên cứu thêm Phần I:Tổng quan Bacillus thuringiensis 1.1 Tóm tắt lịch sử nghiên cứu ứng dụng Bacillus thuringiensis Lịch sử Bacillus thuringiensis ... cánh cứng, hai cánh số loài phụ Bacillus thuringiensis var.berliner, Bacillus thuringiensis var.thompsoni Khác với loài Bacillus thuringiensis, loài phụ Bacillus thuringiensis var.israelensis đợc ... phân loại loài phụ Bacillus thuringiensis var.israelensis Bacillus thuringiensis var.israelensis loài phụ loài Bacillus thuringiensis De Bajac ngời phân loại loài phụ Bacillus thuringiensis var.israelensis...

Ngày tải lên: 28/04/2013, 19:38

... từ Bacillus thuringiensis var.israelensis cần phải đợc nghiên cứu thêm Phần I:Tổng quan Bacillus thuringiensis 1.1 Tóm tắt lịch sử nghiên cứu ứng dụng Bacillus thuringiensis Lịch sử Bacillus thuringiensis ... cánh cứng, hai cánh số loài phụ Bacillus thuringiensis var.berliner, Bacillus thuringiensis var.thompsoni Khác với loài Bacillus thuringiensis, loài phụ Bacillus thuringiensis var.israelensis đợc ... phân loại loài phụ Bacillus thuringiensis var.israelensis Bacillus thuringiensis var.israelensis loài phụ loài Bacillus thuringiensis De Bajac ngời phân loại loài phụ Bacillus thuringiensis var.israelensis...

Ngày tải lên: 25/07/2013, 15:42

... sinh trởng v tạo th nh b o tử chủng Bacillus thuringiensis Chủng giống Bacillus thuringiensis 8-2 Bacillus thuringiensis G3 Bacillus thuringiensis AM4 Bacillus thuringiensis H-H1 8-2, G3, AM4, H-H1 ... xylostella) (Heliothis armigera) Bacillus thuringiensis 8-2 0,42 1,09 130 Bacillus thuringiensis G3 0,.23 2,30 130 Bacillus thuringiensis AM4 0,87 3,44 130 Bacillus thuringiensis H-H1 0,07 4,15 130 ... chủng Bacillus thuringiensis Theo Dulmage (1981) việc sản sinh protein tinh thể độc Bacillus thuringiensis phụ thuộc nhiều v o điều kiện nuôi cấy v th nh phần môi trờng Vậy chủng Bacillus thuringiensis...

Ngày tải lên: 29/08/2013, 08:17

... identified by NMR-monitored titrations of Bacillus circulans xylanase [13] and X-ray analysis of crystals of catalytically incompetent mutants of the xylanases of Bacillus subtilis (XBS) and Aspergillus ... that, in the final sample, less than 30% of the X6 was hydrolyzed After 60 of incubation, the reaction was terminated For XBS samples, this was carried out by the addition of 20 lL of NaOH (2.00 m) ... is only half that of the wild-type XAN However, a loss of activity on X6 in the set of purified XAN mutants does not appear to be correlated with a weakening of the SBS For example, Y29A does not...

Ngày tải lên: 14/02/2014, 19:20

... terminated by the addition of EDTA to a final concentration of 26 mM After the addition of D2O to a final concentration of 10% (v/v), the samples were analysed by NMR spectroscopy Assay of lactate dehydrogenase ... affinity of the enzyme for that cofactor and the apparent absence of a redox process as part of the catalytic cycle The substrate binding site of the type II enzyme remains veiled However, a patch of ... Shigella sp.) of the proteobacteria group 10) are devoid of genes for the biosynthesis of IPP and DMAPP The same is true for the genome of the archaeon N equitans No putative orthologs of the type...

Ngày tải lên: 19/02/2014, 13:20

... Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipV Cloning of sipV Cloning of sipV Cloning of sipV ... Cloning of sipV Cloning of sipV Cloning of sipV Construction of pOpacVh Construction of pOpacVh Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW ... Construction of pOpacWh Construction of pOpacWh Construction of pOpacSh Construction of pOpacSh Construction of pOpacTh Construction of pOpacTh Construction of pOpacBh Construction of pOpacBh Construction...

Ngày tải lên: 21/02/2014, 03:20

... One unit of isomerase activity is defined as the amount of enzyme that produces mmol of product per under the assay conditions Activation of apo-BLXI by metals To examine the effect of bivalent ... the presence of Co2þ or Mn2þ are interesting With a similar inactivation mechanism, Ea of inactivation of T thermosulfurigenes XI is only 490 kJ·mol21 in the presence of Table Effect of metals on ... stability and lower Ea of inactivation of BLXI in the presence of Mg2þ reflect the fact that the enzyme binds Mg2þ with lower specificity (the Kact of Mg2þ is 10 times higher that that of Co2þ or Mn2þ...

Ngày tải lên: 22/02/2014, 07:20

... refmac5 [31] of the ccp4 suite of programs [32] Five per cent of the unique reflections were reserved for the calculation of free R and for the validation and monitoring of the progress of refinement ... PLP content of these preparations (0.2 mol per mol of subunit in Y51F and 0.6 mol per mole of subunit in Y61F bsSHMT, compared with mol per mole of subunit in bsSHMT) The PLP content of Y61A bsSHMT ... Fig (A) Absence of spectral changes in Y61A bsSHMT on addition of Gly or Gly + FTHF Absorption spectra of Y61A bsSHMT (1 mgÆmL)1) in final buffer D (d) and on addition of 50 mM of L-Ser ⁄ Gly (—)...

Ngày tải lên: 16/03/2014, 06:20

... representing the PSBD of the dihydrosuccinyltransferase chain of the 2-oxoglutarate dehydrogenase complex of E coli [12] and of the dihydroacetyltransferase chain of the PDH complex of B stearothermophilus ... structure of PSBD was subsequently determined and compared with that of the PSBD in the crystal structure of the PSBD–E3 complex [28], thereby allowing a better estimate of the extent of molecular ... N-terminus of helix I of the domain and the three residues in loop L2 contributing to the E3-binding site Backbone dynamics of PSBD The region (Met132 to Ser134) before the start of helix I of the...

Ngày tải lên: 16/03/2014, 18:20

... S5 Binding of ATP to McsA using the ITC method Table S1 List of primers used for cloning of McsB and McsA, and for creating site-specific mutants of Tyr155 and Tyr210 of McsB McsB of Bacillus anthracis ... fluorescence of McsB at 340 nm was monitored in the presence of increasing concentrations of McsA, which allowed determination of the Ka value of 5.0 (± 0.5) · 105 m)1 and the stochiometry of 0.9, ... inactive [11] Comparison of the two-component system of B anthracis with those of other members of the Bacillus cereus group shows that B anthracis appears to lack some of the important histidine...

Ngày tải lên: 23/03/2014, 06:20

... mgÆmL)1 chloramphenicol Blue stained colonies showing deregulation of xynP¢::lacZ were picked for verification of the deletion of ccpA by Western blotting For complementation of WH440 ccpA was amplified ... SPR at lm of HPrSerP or lm of CrhP so that about 20% of the immobilized CcpA is complexed The addition of FBP or Glc6-P at millimolar concentrations led to increased complex formation of HPrSerP ... nM of CcpA with or without 50 lM of HPr (B) Titration of cre with CrhP in the presence of 10 nM CcpA The baseline response was found for 10 nM of CcpA with 50 lM of Crh The CrhP concentration for...

Ngày tải lên: 23/03/2014, 13:20

... protein minus derivative of Bacillus thuringiensis subsp kyushuensis Curr Microbiol 45, 133–138 17 Hofte H & Whiteley HR (1989) Insecticidal crystal pro¨ teins of Bacillus thuringiensis Microbiol ... of Cry4Aa facilitated the formation of protein inclusions of fused heterologous protein in E coli Therefore, the block sequence may be one of the factors responsible for the crystallization of ... Cry3Bb1 of Bacillus thuringiensis Acta Crystallogr D Biol Crystallogr 57, 1101–1109 11 Grochulski P, Masson L, Borisova S, Pusztai-Carey M, Schwartz JL, Brousseau R & Cygler M (1995) Bacillus thuringiensis...

Ngày tải lên: 29/03/2014, 09:20