aeruginosa ralstonia solanacearum and chromobacterium violaceum and the discovery of their lectins
The 11th form non-english majors’ level of satisfaction with their reading comprehension lessons at phan boi chau specialized upper secondary school, nghe an
Ngày tải lên :
07/11/2012, 14:54
... administered to the experimental subjects in order to gather their opinions and suggestions of CS There are three categories: their attitudes towards the program as a whole, their preference of the five ... background knowledge and improve the other language skills And the majority of the subjects (75%) indicated their confidence in gaining knowledge of words better Most of them supposed that they learnt ... proficiency before and after the experiment Thus, the results of these tests were analyzes using the Excel Spread Program to calculate the Independent t-test 1.1 The results and discussion of the...
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THE 11TH FORM NON ENGLISH MAJORS’ LEVEL OF SATISFACTION WITH THEIR READING COMPREHENSION LESSONS AT PHAN BOI CHAU SPECIALIZED UP
Ngày tải lên :
07/09/2013, 13:48
... learning and therefore, better their understanding and improve their reading skills Reading activities are considered good if: - The students are fond of the topic and eager to read - The students ... French and Russian The school selects all the best teachers and students from school in the province The main duty of the school is to train and foster talents for the province and the country The ... suitable and relevant the teaching and learning styles are to the students’ interests, habits and learning ability…, the more they can get from the lesson Therefore, they are more satisfied with their...
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Tài liệu Báo cáo Y học: Interaction of decorin with CNBr peptides from collagens I and II Evidence for multiple binding sites and essential lysyl residues in collagen pot
Ngày tải lên :
21/02/2014, 15:20
... spectra of a recombinant decorin purified in the absence of chaotropic agents, with the exception of the wavelength of the minimum (215–216 instead of 218 nm) and very different to the spectrum of the ... presence of chondroitin/dermatan sulfate PGs at the d and e bands in the gap zone of the fibrils formed by the quarter staggered array of type I collagen molecules, and the presence of keratan ... On the contrary, no influence of detergents was found, as determined by the addition of 1% Triton to the binding solution To further characterize the nature of the interaction between decorin and...
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Báo cáo khoa học: Comprehensive interaction of dicalcin with annexins in frog olfactory and respiratory cilia pdf
Ngày tải lên :
07/03/2014, 05:20
... lacked the N-terminal regions Arrowheads show the sites cleaved and the molecular masses of the rest of the cleaved peptides Arrows show the N-termini of the 35 kDa forms of annexin A1 and annexin ... (B) These two controls are shown as thin dotted lines in (C) and (D) The result obtained in the presence of dicalcin and all of the annexins (E) was compared with the calculated sum of each of the ... annexin A4 and annexin A5 to identify which annexins were in the fraction of the 38 kDa and the 35 kDa proteins Cloning of annexin cDNAs On the basis of the partial amino acid sequences of the proteolytic...
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Báo cáo khoa học: Interaction of DAPI with individual strands of trinucleotide repeats Effects on replication in vitro of the AATÆATT triplet docx
Ngày tải lên :
16/03/2014, 23:20
... triplet sequences, the specificity of the observed effects confirms the higher selectivity of DAPI for the minor-groove of the ATT-triplet with respect to all the other individual strands of trinucleotide ... also supported by the observation that the first 10 bases of the GC-rich random sequence of the AAT- and ATT-templates are copied without any pausing in relation to the drug-free syntheses This provides ... attributed to the (ATT)10 region (located at the 5¢-end of the ATT-template) folding into a hairpin-like structure The double helix of the hairpin stem, stabilized by the binding of DAPI into the minor-groove...
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Báo cáo khoa học: Interaction of 42Sp50 with the vegetal RNA localization machinery in Xenopus laevis oocytes ppt
Ngày tải lên :
23/03/2014, 03:20
... above and analyzed by western blotting for the presence of known localization factors Input corresponds to 1% of the material used in the pulldown experiment The presence of the TAP-Vg1RBP band ... band in the anti-Staufen blot is a result of the strong binding of secondary anti-rabbit serum to the protein-A moiety of the TAP-tag Anti-CBP serum was used to show the expression of the TAP-tag ... should be part of one and the same complex; on the basis of the experimental results obtained in the present study, we cannot exclude the possibility that ePAB and FRGY2 are indeed part of different...
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Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot
Ngày tải lên :
23/03/2014, 04:21
... particularly H4, and barely interacts with the region of H1 and the O-methyl group The crystal structure of EW29Ch with lactose showed that the O2, O3 and O4 atoms of the galactose residue of lactose ... NH of Trp33 disappeared The broadened signals sharpened at the position of the second signal at [sugar] ⁄ [EW29Ch] = 3–4 and the resonance signal of the side-chain NH of Trp33 appeared at the ... residue and subdomain c of EW29Ch was not observed; (b) the B-factor of the side chain of Lys105 was high, indicating that the side chain of Lys105 is flexible; (c) in this NMR study, the Kd of the...
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Báo cáo Y học: Exploration of the diaphorase activity of neutrophil NADPH oxidase Critical assessment of the interaction of iodonitrotetrazolium with the oxidase redox components doc
Ngày tải lên :
31/03/2014, 21:21
... 500 nm and 550 nm, which was characteristic of the monoformazan, i.e the fully reduced product of INT [15,17] In the following aeration of the medium, the absorbance of the spectral bands decreased, ... inward The rates of oxygen uptake were deduced from the slopes of the tangents to the oxygraphic traces, and the contact points of the tangents with the curves were used to determine the O2 concentrations ... [13], either by measurement of the rate of production of O2– or the rate of O2 uptake INT diaphorase activity was assayed by the rate of reduction of INT into formazan in the presence of superoxide...
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Báo cáo y học: "Direct interaction of immunoglobulins with synovial fibroblasts: a missing link in the pathogenesis of rheumatoid arthritis" potx
Ngày tải lên :
09/08/2014, 06:22
... transformation), that leads to alteration in their apoptotic response, the attachment of these cells to articular cartilage and subsequently to the degradation of the cartilage matrix [11, 13] This notion ... as the SCID-mouse in vivo model of cartilage destruction Although a number of molecular pathways have been identified that contribute to the stable activation of RASFs, the precise cause and ... before being exposed to the immunoglobulins Consequently, the data suggest that the local stromal environment in the joint (and based on previous data from the group other organs as well [14])...
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báo cáo khoa học: " Gene expression profiling in susceptible interaction of grapevine with its fungal pathogen Eutypa lata: Extending MapMan ontology for grapevine" pdf
Ngày tải lên :
12/08/2014, 03:21
... advantage of using MapMan is no doubt the ease and speed of the analysis The visualization of the genes distribution among the phytohormones confirms the large involvement of genes linked to auxins and ... analysis The general trend of these classes is the same as reported in Figure of [21], in which it is evident that all of them are prevalently induced from stage A to B and then mostly repressed The ... but they are usually restricted to specific parts of the metabolism However, it is often interesting to get a quick and complete overview of the whole data set, especially at the start of the...
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Interaction of EcoRI with noncognate DNA sequences computational investigation of dynamics of protein water and DNA conformation
Ngày tải lên :
09/09/2015, 18:49
... the operon in 1961 [8] Their theory was based on their observations of induction of the lac gene The isolation and characterization of the lac repressor, a protein, and the discovery that it actually ... chemical nature of a gene was and what precisely it did The answers to these questions were slowly revealed with the discovery of DNA as the genetic material [4] and the discovery of the double-helical ... showing the exclusion of water molecules at the interface of the protein and DNA during the formation of the specific complex (Adopted from [92]) binding [60] The structures of the specific and nonspecific...
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Interaction of scribble with zonula occludens and intermediate filament proteins
Ngày tải lên :
14/09/2015, 08:24
... contribution of reagents but also his support during the course of my project and advice throughout the writing and revision of the publication of my work My candidature in the Institute of Molecular and ... through the relief of this suppression by the binding of Cdc42-GTP to Par6 (Yamanaka et al., 2001) The coordinated spatial and temporal regulation of the Par complex activity and that of the other ... Conversely, the basal activity of the Scrib complex is antagonized by the Par and Crb complexes apically and therefore restricted to the basal domain As such, opposing functions of the Par-Crb and Scrib...
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Báo cáo khoa học: DNA mediated disassembly of hRad51 and hRad52 proteins and recruitment of hRad51 to ssDNA by hRad52 pot
Ngày tải lên :
16/03/2014, 14:20
... changes associated with the aggregation states of hRad51 and hRad52 in the presence of 121-mer ssDNA and hRad52 mediated assembly of the ssDNA–hRad51 complex The changes in the protein aggregation ... indicates the formation of large complex in the presence of hRad51, hRad52 and ssDNA presence of hRad52 renders much better binding of hRad51 to ssDNA even at lower concentrations of the latter, thereby ... fraction of the assay Both of the assays suggested that addition of ssDNA facilitates significant level of disaggregation in hRad51 To reconfirm the DNA mediated disaggregation of hRad51, we analysed the...
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Intein mediated generation of n terminal cysteine proteins and their applications in live cell bioimaging and protein microarray
Ngày tải lên :
08/11/2015, 16:30
... elucidation of the splicing mechanism and the identification of the key amino acid residues involved in the scission and ligation of the peptide bonds have facilitated the molecular engineering of artificial ... ASN O Figure Mechanism of intein splicing at the C-terminal junction of the Ssp DnaB intein, with Asn as the last amino acid of the intein and Cys as the first residue of the target protein Self-cleavage ... into the cell, where the chemoselective native chemical ligation reaction occurred between the thioester of the small molecule and the N-terminal cysteine of the protein, giving rise to the resulting...
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Báo cáo y học: " Antagonistic interaction of HIV-1 Vpr with Hsf-mediated cellular heat shock response and Hsp16 in fission yeast (Schizosaccharomyces pombe)" potx
Ngày tải lên :
13/08/2014, 09:20
... Analysis of hsp16 expression in S pombe Q1649 strain, in which the hsp16 gene is tagged with GFP and is under the control of its native promoter [35], demonstrated that both the wild type Vpr and the ... overproduced Hsf1 (Fig 3B) Together, results of these experiments suggest that Hsf is the key cellular regulator of heat shock-mediated suppression of the Vpr activities Page of 14 (page number not for ... noticed in the examination of two Vpr mutations The R77Q and I74A mutants, which separate the apoptosis and G2 arrest induced by Vpr [24,54], showed no separation between the G2 induction and apoptosis...
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Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
Ngày tải lên :
20/02/2014, 02:21
... consequence of the mutagenic replacements Effects of the His120Asn and His145Asn mutations on the affinity of metal binding to arginase II To further examine the effects of the His120fiAsn and His145fiAsn ... by His120 and His145, which are equivalent to the histidines at position 101 and 126 in the sequence of arginase I [10] However, they are clearly differentiated by the consequences of their replacements ... electrostatic repulsion between the a-carboxyl group of the amino acid and the introduced aspartic residue at position 149 On the other hand, as agmatine lacks the a-carboxyl group, there would be no impediment...
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Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc
Ngày tải lên :
20/02/2014, 23:20
... peak at 7.65 mL on the elution profile Increase of the temperature of incubation was accompanied by the simultaneous increase of the peak eluted at 7.65 mL and decrease of the peak eluted at 8.65 ... polymerization of intact actin and aggregation of heated actin Interaction of HSP25 with intact actin Fig Effect of heating on the kinetics of polymerization (A) and saltinduced increase of the light ... (Fig 6A) Indeed the critical concentration of actin was equal to 0.25 ± 0.07; 0.21 ± 0.05, and 0.15 ± 0.06 lM in the absence of HSP25, and in the presence of the wild type HSP25 and its 3D mutant,...
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Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx
Ngày tải lên :
06/03/2014, 00:21
... interaction in the presence of mm ATP On the other hand, 2-oxoglutarate did not influence the GlnK–TnrA interaction, either alone, in the absence of divalent metals, or in combination with ATP and Mg2+ ... lower panels show the binding isotherm and the best-fit curve according to the three sequential binding sites model BIAcore analysis of the GS–TnrA interaction The results of the immunoprecipitation ... with their receptors [17] The divalent cations Mg2+ or Mn2+ were previously shown to negatively affect the binding of ATP to GlnK [12] Therefore, we investigated the binding of TnrA to GlnK in the...
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Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt
Ngày tải lên :
07/03/2014, 16:20
... Consequently, the molecular studies of the interaction of SAgs with their specifics ligands will not only advance understanding of the physiological mechanisms of these molecules, but may lead to the development ... treatment of several pathologies and because of the potential use of the toxins as biological weapons Alteration of their MHC and TCR binding capacity by site directed mutagenesis could be useful in the ... species; reduction and alkylation, and mutation of the Cys implicated in the intermolecular disulfide bridge Analysis of the three-dimensional structure of SSA [31] to determine which of the remaining...
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