adipose stem cell engineering characterization and current application in otolaryngology

... problem in drug discovery Despite the promise of hESCs in tissue engineering and cellular transplant therapy, the gap between scientific research and clinical applications for safe and effective stem ... factors and anchoring substrates are the underlying principles of these systems, which try to mimic the microenvironments of the hESCs Indeed, keeping in mind the origin of hESCs, the neighbouring cells ... would in nature, and in similar proportions to that of in vivo ECM is formed from many different structural macromolecules and plays a crucial role in maintaining tissue structure and modulating cell...

Ngày tải lên: 11/09/2015, 10:06

... Christopher Stephen Hughes and Prof Gilles Lajoie * Protein also found in GFR and standard Matrigel, based on work by Hughes et al 2009 † Murine protein is a homolog of human protein 50 ... metalloproteinase with thrombospondin motifs 1*† Transforming growth factor-beta-induced protein ig-h3 74665 122841 234698 Isoform of Extended synaptotagmin-1*† Isoform of Myoferlin*† 99.80% ... 105340 Keratin, type I cytoskeletal 10 Keratin, type II cytoskeletal Collagen alpha-1(I) chain*† Collagen alpha-2(I) chain*† EMILIN-1*† Fibrillin-1* Isoform of Collagen alpha-1(XII) chain Isoform...

Ngày tải lên: 11/09/2015, 10:06

... contains more Fn (by immunofluoresence comparison, section 3.3.2), it would be expected that the 52 increased presence of Fn would aid in increasing integrin binding and hence cell binding This ... due to the increased presence of DCN and BGN in the DxSNP40/DNase and FcNP40/DNase matrices interfering with TGFB signaling for the hESCs, since DCN and BGN both bind active TGFB, and could lead ... amounts of proteins within and their complexity (refer to section 3.3), not allow the adherence of hESCs and also not maintain hESCs in a pluripotent state This observation is surprising, since these...

Ngày tải lên: 11/09/2015, 10:06

... of hESCs, and so, in order to obtain successful Oct-4 immunostaining, the cells have to be first permeabilized using 0.2% Triton-X 100 - this extra processing step results in markedly increased ... trophoectoderm lineages [10], indicating the crucial role that Oct-4 plays in maintaining the pluripotent state of ES cells As Oct-4 is a transcription factor, naturally it is found within the nuclei ... down-regulated in trophoectoderm and primitive endoderm of the mouse embryo Later, it is maintained in the embryonic ectoderm at the cylinder stage, and down-regulated at gastrulation in an anterior-posterior...

Ngày tải lên: 11/09/2015, 10:07

... at increasing the population doublings and also in 84 maintaining the pluripotent characteristics of the hESCs during their propagation Once again, hESCs were cultured onto Matrigel, DxSDOC and ... Stem Cell Bank In this protocol, the hESCs formed embryoid bodies using non-adherent cell culture plates and were transferred onto laminincoated plates and incubated in neural induction medium ... In vitro induced differentiation hESCs cultured on DxSDOCDOC for 13 passages were induced into neural differentiation using a protocol modified from WiCell Research Institute, National Stem Cell...

Ngày tải lên: 11/09/2015, 10:07

... current cell culture methods employed during hESC culture To attain the goal of clinical application in tissue engineering, stem cell transplant therapy and also drug discovery, hESCs have to be maintained ... Kaukinen K, Maki M, Lindfors K Extracellular transglutaminase has a role in cell adhesion, whereas intracellular transglutaminase is involved in regulation of endothelial cell proliferation and ... facilitate cell growth by promoting cell signaling and growth factor availability However, subsequent staining and gel electrophoresis also showed that in addition to Fn and collagen, which aids in cell...

Ngày tải lên: 11/09/2015, 10:07

... B - cell differentiation from a human pancreatic cell line in vitro and in vivo Mol Endocrinol 15, 476 - 483 Dzierzak, E et al (1998); Embryonic beginnings of definitive hematopoietic stem cells ... 88 - 95 Itkin - ansari, P., et al (2001); PDX - and cell - cell contact act in synergy to promote d - cell development in a human pancreatic endocrine precursor cell line Mol Endocrinol 14, 814 ... circulating human embryonic blood stem cells Blood 96, 1740 - 1747 IItskovitz - Eldor, J and et al (2000).; Differentiation of human embryonic stem cells into embryoid bodies comprising the three...

Ngày tải lên: 02/04/2014, 22:20

... LIF/Stat3 pathway for murine ES cells [3], and bone morphogenetic protein [4] and the Wnt pathway [5] for both mouse and human stem cells Intrinsic factors that maintain selfrenewal include the transactivators ... released4ES cellsSox17, Brac and Mash1 during EB formation of control and mRNA mRNA levels of Sox17, Brac and Mash1 during EB formation of control and released ES cells (ES re) Control and released ES cells ... modifications in TSA-treated ES cells and EBs Analysis of bulk histone modifications in TSA-treated ES cells and EBs Global levels of histone H3 acetylation (acH3), and lysine (3mK4) and lysine 27 (3mK27)...

Ngày tải lên: 14/08/2014, 08:21

... analogs on APAP and H2O2-induced on cell death in TAMH cells 112 4.3.2 Effect of lower dosage of -T3 and -T3 against APAP and H2O2-induced injury on cell viability in TAMH cells 118 4.3.3 ... effective in preventing both toxicants induced injuries -T3 preserved cell viability by acting against the build-up of ROS and its downstream pathway which inhibited the injury and initiation ... of -TP, -T3 in cell viability after APAPand H2O2-induced injury in TAMH hepatocytes 120 Figure 32 Effects of -TP, -T3 in cell viability after APAP- and H2O2induced injury in TAMH hepatocytes...

Ngày tải lên: 09/09/2015, 11:21

... active in ES cells and that mature Lefty2 proteins are secreted into and deposited in the ES cell microenvironment 79 2.1.2.8 LEFTY2 maintains ES cell self renewal by inhibiting Nodal signaling ... Supplementing Lefty2 RNAi cultures with recombinant Lefty1 protein was able to block reduction in level of alkaline phosphatase staining induced by Lefty2 RNAi Alkalline phosphatase staining was ... differentiate the ES cells The cells were then dissociated with trypsin and replated at single cell density to allow the ES cells to expand into colonies It was interesting to note that the number...

Ngày tải lên: 11/09/2015, 16:02

... signals dictate embryonic stem cell fates is also not entirely clear Conflicting roles of Nodal signaling in both the maintenance of ES cell self renewal and induction of ES cell differentiation have ... manner in vitro is addressed Interestingly, it was found that Nodal signaling is able to arbitrate at least three distinct fate decisions in ES cells Perturbation of endogenous signaling in ES cells ... elicit effects in embryos in a dose dependent manner In this thesis, the possibility that Nodal signaling might, as in the in vivo scenario, likewise influence ES cell fate decision in a dose dependent...

Ngày tải lên: 11/09/2015, 16:02

... were maintained on uncoated cell culture dishes in ES cell medium containing DMEM/20% ES FBS (Invitrogen, Carlsbad, CA), 0.1 mM MEM nonessential amino acids (Invitrogen), 2mM L-glutamine (Invitrogen), ... days growth in selective medium, few cells remained in the untransfected control wells indicating that selection was effective The surviving cells were trypsinized and resuspended in ES cell medium ... Protein extraction and western blotting To obtain protein extracts, cells were trypsinized from culture dishes harvested in chilled PBS, centrifuged at 10,000g for at °C, washed again in PBS and incubated...

Ngày tải lên: 11/09/2015, 16:02

... profiling of human and murine ESCs identifies divergent paths required to maintain the stem cell state Stem Cells 23, 166-185 Wieduwilt Matthew J., (2003) SMAD3 in embryonic patterning, mesoderm induction ... beta2microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells Stem Cells 22, 659-668 Matsuda, T., Nakamura, T., Nakao, K., Arai, T., Katsuki, M., Heike, T., and ... embryonic stem cells, Nat Cell Biol 10, 353-360 Jinnin, M., Ihn, H., and Tamaki, K (2006) Characterization of SIS3, a novel specific inhibitor of Smad3, and its effect on transforming growth...

Ngày tải lên: 11/09/2015, 16:02

... pluripotent embryonic stem (ES) cell lines The derivation of pluripotent cell lines from blastocysts was first achieved in the murine system in 1981 by Martin Evans and Matthew Kaufman and independently ... 6bromoindirubin-3'oxime (BIO) It was demonstrated that pluripotency can be maintained in both mouse and human ES cells in the presence of this inhibitor Interestingly, Lefty A and Lefty B are inducible ... findings by culturing human ES cells with a combination of human recombinant ActRIB, ActRIIB and Cripto, thereby strengthening the idea that intact Nodal/Activin signaling is important in maintaining...

Ngày tải lên: 11/09/2015, 16:02

... protein Member of TGFsuperfamily Involved in Nodal signaling Nodal antagonist • • • • • Rex2 • Zinc finger protein • • • Zfx • • • a-catenin • Zinc finger protein Transcription factor Link to ... -catenin (Catnb) Dot1l Lef1 Gata3 • • • • • • • • complex Involved in Wnt signaling Subunit of Cadherin protein complex Intracellular mediator of canonical Wnt signaling Non-SET domain protein Histone ... expression pattern between ES cells and differentiated cells Reduced expression during retinoic acid induced differentiation of F9 cells High expression in ES cells Link to canonical Wnt pathway...

Ngày tải lên: 11/09/2015, 16:03

... Therefore, the incorporation of BM-MSCs in skin grafts holds a great potential in promoting wound healing and skin regeneration in full-thickness skin defect Recent advances in tissue engineering techniques ... periphery through cellular migration and proliferation Endothelial migration during angiogenesis involves binding integrin domains within the provisional fibrin-fibronectin matrix in a similar manner ... staining of involucrin in the epidermis of different experimental groups on post-operative day 10 A, C, E, G: Staining of involucrin with green fluorescence by anti-rat and human involucrin antibody...

Ngày tải lên: 12/09/2015, 09:10

... finding is carried out in order to find out the ways of translating of non – finite clauses Finally, giving some implications on teaching, learning and translating non – finite clauses is done ... understanding,using and translating English non – finite clauses may objectives and some linguists’ theoretical viewpoints about the be significant and important to learners of Engish In reality, ... CHAPTER FINDINGS AND DISCUSSION 4.1 WAYS OF TRANSLATING NON – FINITE CLAUSES By studying English non – finite clauses and their Vietnamese equivalents in English novels, short stories, books and public...

Ngày tải lên: 26/11/2013, 13:19

... great interest both from an environmental (geochemical) and an industrial point of view Most solid phases in natural water contain aluminium oxides Alumina plays an important role in regulating ... alumina and other mineral solids in the presence of other complexing agents such as nitrilotriacetic acid and ethylenediaminetetraacetic acid has also been studied and was found to increase in ... environment, several applications in water treatment technology and also its wide industrial application, the understanding of the chemistry of alumina in water is necessary in order to fully comprehend...

Ngày tải lên: 15/03/2014, 23:22

... gas-sensing performance was systematically investigated by a HW-30A gas-sensing measurement system (Henan Hanwei Electronical Technology Co., Ltd.) The fabrication and testing principle of the gas sensor ... working temperature of 250 °C and 40% relative humidity (RH) An appropriate amount of ethanol vapor was injected into the closed chamber by a microinjector, and the sensor was exposed to air again ... diameter of about 10-15 nm and a length of about 200 nm After being calcined at 300 °C for h, the sample still maintains the rodlike 1-D morphology, as is shown in Figure 3c and d Compared with the...

Ngày tải lên: 19/03/2014, 16:48

... proteins by arginine-rich intracellular delivery (AID) and Tat-protein transduction domain has been reported in onion and corn root tip cells [28,29] Cationic oligopeptides such as polyarginine ... CPPs and their cargo [7,14,24] Interestingly, most investigations involving CPPs have been carried out in mammalian cell lines and there are only few reports on translocation of CPPs in plant cells ... complex in the cellular milieu and its further fate in plant cells Future investigations will focus on answer2410 A Chugh and F Eudes ing questions such as how the combined use of cell permeabilizing...

Ngày tải lên: 23/03/2014, 07:20