adipogenic differentiation of adult equine mesenchymal stromal cells

Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc

Ngày tải lên : 30/03/2014, 04:20

... evidence of transdifferentiation by MET in the process of hepatic differentiation of AT-MSCs No previous report has demonstrated evidence of transdifferentiation of committed adult stem cells In ... Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta Stem Cells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation of mesenchymal ... the process of hepatic differentiation from AT-MSCs Although further investigations are required to elucidate the molecular mechanism of transdifferentiation of AT-MSCs into hepatic cells, the...

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Báo cáo khoa học: "Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury" potx

Ngày tải lên : 07/08/2014, 23:22

... Results Differentiation test of ASCs Adipogenic differentiation of ASCs was apparent after weeks of incubation with adipogenic medium By the end of the third week, half of the cells contained Oil red ... issue of transdifferentiation Stem Cells Dev 2004, 13, 121-131 Cowan CM, Shi YY, Aalami OO, Chou YF, Mari C, Thomas R, Quarto N, Contag CH, Wu B, Longaker MT Adipose-derived adult stromal cells ... characterization of mesenchymal progenitor cells from chorionic villi of human placenta Cytotherapy 2004, 6, 543-553 Jaiswal N, Haynesworth SE, Caplan AI, Bruder SP Osteogenic differentiation of purified,...

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Investigation of osteogenic characteristics of human adipose derived stromal cells

Ngày tải lên : 08/11/2015, 17:01

... is the Mesenchymal stem cell variably called Mesenchymal stromal cells (MSCs) 3.2 Mesenchymal stromal cells for tissue regeneration MSCs are cells of mesodermal origin and have been shown to be ... good source of multipotent adult stem cells is the adipose tissue Cells isolated from these, known as ADSCs (Adipose Derived Stromal Cells) have been shown to possess the property of being multipotent ... can give rise to cells types of different lineages, including those of the osteogenic lineage (Zuk PA et al., 2002) Compared to the other sources of adult stem cells, these cells are found in...

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Báo cáo y học: " High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells." ppsx

Ngày tải lên : 10/08/2014, 10:20

... osteogenesis and vascularization of mesenchymal stem cells Cells Tissues Organs 2009, 190:185-193 Rodbell M: The metabolism of isolated fat cells IV Regulation of release of protein by lipolytic hormones ... DR: Multilineage potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Pittenger MF, Mosca JD, McIntosh KR: Human mesenchymal stem cells: progenitor cells for cartilage, bone, ... these cells were evaluated for markers of endothelial differentiation Yang et al Journal of Biomedical Science 2011, 18:59 http://www.jbiomedsci.com/content/18/1/59 Page of Figure Adipogenic differentiation...

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Characterization of human adipose tissue derived adult multipotent precursor cells

Ngày tải lên : 12/09/2015, 09:55

... CHARACTERIZATION OF HUMAN ADIPOSE DERIVED ADULT MULTIPOTENT PRECURSOR CELLS LEONG TAI WEI DAVID (Bachelor of Chemical Engineering, Hons) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ... osteo -differentiation of ADSC xi List of Tables Table 2.1 Differentiation potentials of ADSC Table 4.1 Supplements in osteogenic and adipogenic induction cocktail Table 6.1 Nomenclature of the ... characterization of other potential sources of stem cells which might avoid these issues altogether 1.1.2.1 Adult stem cells Stem cells are loosely defined as self-renewing progenitor cells that can...

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Báo cáo y học: " Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells" pdf

Ngày tải lên : 13/08/2014, 13:20

... dilutions of DCs, from 10,000 cells per well to 313 cells per well, with × 105 allogeneic CD4 T cells in a 96-well U-bottomed plate in a total volume of 200 µl for days The proliferation of T cells ... function of DCs Polarization of naïve Th cells into Th1 cells is critical for the induction of cellular immunity against intracellular pathogens and cancer cells The observed impairment of the ... cells not only stimulates T-cell proliferation, but also polarizes differentiation of naïve T helper (Th) cells into IFN-γ-producing Th1 or IL4-producing Th2 effector cells [6,7] Production of...

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Báo cáo y học: " Gene expression profiling of human prostate cancer stem cells reveals a pro-inflammatory phenotype and the importance of extracellular matrix interactions" pps

Ngày tải lên : 14/08/2014, 08:21

... cancer cells PTL induces apoptosis in primitive cancer cells Percent viability of prostate cancer cells and cells from a patient with BPH treated with increasing concentrations of PTL Cells were ... stellate cells highlighted the importance of culture microenvironment and the appropriate use of feeder cells in co-culture Comparison of transcriptional profiles from hepatic stellate cells cultured ... phenotype CD133+/α2β1hi (high expression of α2β1 integrin) and comprising less than 0.1% of the tumor mass, has many of the properties of cancer stem cells [4] In particular, self renewal, extended...

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Engineering and characterization of human renal proximal tubular cells for applications in vitro toxicology and bioartifical kidneys

Ngày tải lên : 09/09/2015, 10:19

... by the transfection The percentage of dead cells was calculated by expressing total number of PI-stained cells as a percentage of total number of DAPI-stained cells which were counted using Image ... properties of primary cells change during passaging, and cells become increasingly senescent Furthermore, dedifferentiation or transdifferentiation processes can occur during the in vitro culture of ... Effects of BMP-7 and BMP-2 on HPTC 67 4.3.1 Effects of BMP-7 on the maintenance of epithelia formed by HPTC 67 4.3.2 Effects of BMP-2 treatment 70 4.3.3 Quantification of α-SMA...

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ENGINEERING THREE DIMENSIONAL CULTURE PLATFORMS FOR HUMAN ADIPOSE DERIVED STEM CELL THERAPY

Ngày tải lên : 09/09/2015, 11:18

... Stem cells in tissue regeneration 2.2.1 Embryonic stem cells 2.2.2 Adult stem cells 2.2.3 Adipose derived stem cells Chapter Chapter v 2.2.4 Hepatic differentiation of adipose derived stem 10 cells ... of stemness marker genes on gelatin 49 microspheres 4.2.4 Adipogenic and osteogenic differentiation of 49 adipose derived stem cells 4.2.5 Hepatic differentiation of adipose derived stem 51 cells ... are one such kind of adult stem cells They are categorized as mesenchymal stem cells (MSCs) and have very similar characteristics to that of bone marrow derived mesenchymal stem cells (BMSCs) (Kern...

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Báo cáo khoa học: "Regeneration of human bones in hip osteonecrosis and human cartilage in knee osteoarthritis with autologous adipose-tissue-derived stem cells: a case series" docx

Ngày tải lên : 10/08/2014, 23:21

... approximately 40 cm3 of packed adipose tissue, fibrous tissue, red blood cells and a small number of nucleated cells An equal volume of digestive enzyme, 0.07% collagenase type 1, composed of several collagenases, ... implanted, autologous mesenchymal stem cells Pain Physician 2008, 11:343-353 Lee JS, Lee JS, Roh HL, Kim CH, Jung JS, Suh KT: Alterations in the differentiation ability of mesenchymal stem cells in patients ... source of multipotent stem cells Mol Biol Cell 2002, 13:4279-4295 13 Peterkofsky B: Bacterial collagenase Methods Enzymol 1982, 82:453-471 14 Salem H, Thiemermann C: Mesenchymal stromal cells: ...

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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx

Ngày tải lên : 18/06/2014, 19:20

... function of tumor-educated myeloid cells was measured by their ability to inhibit the proliferation of autologous T cells in the following Suppression Assay: T cells isolated from 30 mL of PBMC ... shown from Suppression Assays of CD33+ or CD11b+ cells with autologous T cells, respectively * indicates statistically significant suppression of T cells by CD11b+ cells from co-culture (p

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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx

Ngày tải lên : 13/08/2014, 23:20

... Refinement and increased efficiency of these two ESC protocols brings the potential to offer a reliable supply of embryonic stem cells without production of whole embryos or compromising extant ... embryonic stem cells: theoretical approaches Blastomere biopsy and culture Prior to the blastocyst stage, a human embryo at 2–3d post fertilization consists of just 4–8 cells, all of which are ... proceed with much greater likelihood given the uniformity of all sampled cells Given the unknown extent of embryo mosaicism, limitations of single blastomere biopsy have been recognized [22] and...

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Immunological characterization of human umbilical cord lining derived cells and their therapeutic application in a diabetic mouse model

Ngày tải lên : 11/09/2015, 10:02

... possibility of potential tumorigenesis after transplantation Adult stem cells or progenitor cells could also be used for generation of insulinproducing cells through differentiation or transdifferentiation ... unless autologous cells are used The major obstacle of using autologous cells is to obtain sufficient number of cells, which may potentially be solved by the reprogramming of somatic cells into iPSCs ... dying cells and regenerate damaged tissues There have been many studies on adult stem cells, given the variety of their types Based on the theme of the present study, one selected type of adult...

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báo cáo hóa học:" Human fallopian tube: a new source of multipotent adult mesenchymal stem cells discarded in surgical procedures" potx

Ngày tải lên : 18/06/2014, 15:20

... chondrogenic and adipogenic differentiation of hFTs cells 2A) Control hFTs cells (630×) 2B) Osteogenic differentiation (200×) 2C) Chondrogenic differentiation (100×) 2D) Adipogenic differentiation ... Prockop D: Mesenchymal stem/multipotent stromal cells: the state of transdifferentiation and modes of tissue repair – current views Stem Cells 2007, 25:2896-28902 Valtieri M, Sorrentino A: The mesenchymal ... and characterization of potential sources of adult stem cells [7,10,1215], the aim of this study was to isolate, expand, characterize and assess the differentiation potential of MSCs from hFTs Methods...

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Báo cáo y học: "Human infrapatellar fat pad-derived stem cells express the pericyte marker 3G5 and show enhanced chondrogenesis after expansion in fibroblast growth factor-2" potx

Ngày tải lên : 09/08/2014, 10:23

... staining of IPFPderived cells It was therefore interesting that they showed a pattern of expression on a high proportion of the IPFP-derived cells and of epitopes commonly abundant on adult stem cells ... Clonal IPFP-derived cells retained the cell surface characteristics of the parent IPFP cells, which were similar to mesenchymal stem cells The clonal populations of IPFP-derived cells appeared phenotypically ... characterisation, single cells were clonally expanded and stained for a panel of stem cell markers, including 3G5 To allow for the effect of inherent variability in the differentiation potential of cells between...

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Báo cáo y học: "Isolation and characterization of human cells resistant to retrovirus infection" pdf

Ngày tải lên : 13/08/2014, 05:22

... retroviral vectors (that enables infection of a wide variety of cells) have revealed differences in the efficiency of single round infection in cells of differing types and species [11,12] Therefore, ... x 105 Figure cells with an HIV-EGFP vector at a moi = 0.5 Infection of parental and mutant HeLa cells (30-2 and 42-7) Infection of parental and mutant HeLa cells (30-2 and 42-7) cells with an ... parental and mutant cells and extent of HIV Growth Growth rates of parental and mutant cells and extent of HIV integration (A) Growth rates Parental and mutant 30-2 and 42-7 cells were seeded and...

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Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"

Ngày tải lên : 25/10/2012, 11:10

... total amount of pravastatin entrapped in 0.1 ml of the final packed erythrocytes Efficiency of entrapment The percentage of the loaded amount of pravastatin to the total amount of that added ... percentage ratio of the hematocrite value of the final loaded cells to that of the initial packed cells, both measured using equal suspension volumes In vitro characterization of pravastatin loaded ... ml eppendorff tubes, 400 µl of suspension are added to 400 µl of PBS containing the known concentration of the drug and 2.5 mmol of ATP, 2.5 mmol MgCl2 and 2.5 mmol of CaCl2, gently mixing to...

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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx

Ngày tải lên : 19/02/2014, 02:20

... media containing isoform I Both isoforms were also expressed intracellularly, as evidenced by the appearance of protein bands of the expected size upon SDS ⁄ PAGE analysis of the crude extracts ... recombinant isoforms Lane M, molecular mass standards production of the human recombinant isoforms might be enhanced by the presence of metal ions in the growth medium We found that addition of 200 ... the mean of three independent experiments and are presented as copies of the target variant per 109 copies of 18S RNA Quantitation of ACMSD variants in liver, kidney and brain An isoform-speciﬁc...

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