neural differentiation of human adipose tissue derived stem cells

... release of hematopoietic stem cells from the bone marrow into the bloodstream of patients with a variety of malignancies The stem cells are then harvested from the blood as a source of stem cells ... cells Since an SDF-1 gradient determines the direction of mobilization of hematopoietic stem cells and progenitors [6], we examined whether SDF-1 levels are decreased in bone marrow stromal cells ... bones of TNF-transgenic mice stromal cellsstromal cell -derived factor-1 expression in bone marDecreased stromal cell -derived factor-1 expression in bone marrow row stromal cells and bones of TNF-transgenic...

Ngày tải lên: 09/08/2014, 10:23

... while they were treated with AMD3100 reduced the number of harvested cells to that of the negative control The number of cells in the air pouch of AMD3100-treated mice after challenge with 0.9% NaCl ... Decreased systemic production of IL-6 in AMD3100-treated mice may be an indirect effect of inhibition of CXCL12-mediated cell traffic, as this might reduce formation of inflammatory tissue in joints ... increase of thickness of the right and the left ear, divided by the thickness of the ear before challenge, multiplied by 100) The chemotactic index was calculated as the number of migrated cells...

Ngày tải lên: 09/08/2014, 07:20

... phosphorylation of CXCR4, tyrosine phosphorylation of SD-4 occurs in response to SDF-1 stimulation of HeLa cells This tyrosine phosphorylation depends on the time of incubation of the cells with the ... ectodomain of SD-1 of human origin), anti(SD-4) mAb 5G9 (mouse IgG2a; clone 5G9; specific for the ectodomain of SD-4 of human origin); anti-(SD-2) (goat IgG; specific for the C-terminal domain of syndecan-2 ... III-treated HeLa cells In parallel, we investigated whether the reduction of expression of SD-4 on HeLa cells by the use of RNA interference prevented these activations HS removal from HeLa cells or...

Ngày tải lên: 16/03/2014, 18:20

... systems Mechanisms of immune evasion include presentation of a tumor antigen by tumor cells without the necessary co-stimulatory signal [23,24], suppression or anergy of tumor-infiltrating T -cells ... Figure CEA-specific proliferation of human lymphocytes measured by uptake of [3H]-thymidine CEA-specific proliferation of human lymphocytes measured by uptake of [3H]-thymidine T-bodies (labeled ... CEA-expressing cells CEA specific T bodies had startling therapeutic effects in a mouse model of human colon cancer Thus, this vector has potential for redirection of human PBLs to chimeric anti-tumor T cells, ...

Ngày tải lên: 19/06/2014, 08:20

... systems Mechanisms of immune evasion include presentation of a tumor antigen by tumor cells without the necessary co-stimulatory signal [23,24], suppression or anergy of tumor-infiltrating T -cells ... Figure CEA-specific proliferation of human lymphocytes measured by uptake of [3H]-thymidine CEA-specific proliferation of human lymphocytes measured by uptake of [3H]-thymidine T-bodies (labeled ... CEA-expressing cells CEA specific T bodies had startling therapeutic effects in a mouse model of human colon cancer Thus, this vector has potential for redirection of human PBLs to chimeric anti-tumor T cells, ...

Ngày tải lên: 20/06/2014, 04:20

... analysis software (SABiosciences) To study the effect of viral exposure on HD cells, we compared the expression of selected genes in HD-HIV cells versus un-infected HD cells The gene profile of HD-HIV ... plot the graphs Abbreviations MSCs: mesenchymal stem cells; ASCs: adipose tissue derived mesenchymal stem cells; HD: hematopoietic differentiated cells; AIDS: acquired immune deficiency syndrome; ... Benhaim P, Hedrick MH: Human adipose tissue is a source of multipotent stem cells Mol Biol Cell 2002, 13:4279-4295 Woodbury D, Reynolds K, Black IB: Adult bone marrow stromal stem cells express germline,...

Ngày tải lên: 13/08/2014, 01:20

... Spermatogonial Stem Cells (SSCs) Differentiation of germ cells in the testis originates from a constantly renewed small pool of stem cells, termed SSCs located at the basement membrane of the seminiferous ... all of you as my labmates i TABLE OF CONTENTS ACKNOWLEDGEMENTS I SUMMARY IV LIST OF ABBREVIATIONS V LIST OF FIGURES VII LIST OF TABLES VIII CHAPTER 1: INTRODUCTION 1.1 Spermatogonial Stem Cells ... diagram of spermatogonial multiplication and stem cell renewal This scheme probably applies to all mammals except for humans (De Rooij 1983) Stem cells (As) proliferate, renewing the stem cell...

Ngày tải lên: 03/10/2015, 20:57

... (Table 2) The number of CD4+/CD25+ T cells and of CD3+/CD16/56+ NK-like T cells was always below 5% Like in healthy human individuals the proportion of CD3-/CD16+CD56+ NK cells in the peripheral ... A- Photomicrograph view of the patient -derived cell line of the anastomotic relapse A- Photomicrograph view of the patient -derived cell line of the anastomotic relapse Cells were cultured and ... cytolytic activity of patient -derived PBMNC derived from L1 to L6 and L7 to L9 against K562 cells (A) and autologous In vitro (B) In vitro cytolytic activity of patient -derived PBMNC derived from L1...

Ngày tải lên: 18/06/2014, 15:20

... Characterization of cytokine-induced myeloid -derived suppressor cells from normal human peripheral blood mononuclear cells J Immunol 2010, 185:2273-2284 Kusmartsev S, Gabrilovich DI: Effect of tumor -derived ... characterization of human Cd33+ And Cd11b+ myeloid -derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines Journal of Translational ... shown from Suppression Assays of CD33+ or CD11b+ cells with autologous T cells, respectively * indicates statistically significant suppression of T cells by CD11b+ cells from co-culture (p

Ngày tải lên: 18/06/2014, 19:20

... IFATS collection: in vivo therapeutic potential of human adipose tissue mesenchymal stem cells after transplantation into mice with liver injury Stem Cells 2008, 26:2705-2712 30 Sementilli A, Franco ... Use of Laboratory Animals (NIH publication No Page of 13 85-23, National Academy Press, Washington, DC, USA, revised 1996) Animal Grouping and Isolation of Adipose- Derived Mesenchymal Stem Cells ... was added to the homogenized adipose tissue in a ratio of 3:1 (saline: adipose tissue) , followed by the addition of stock collagenase solution to a final concentration of 0.5 units/mL The centrifuge...

Ngày tải lên: 18/06/2014, 22:20

... with isoflurane Am J Vet Res 2003, 64, 1155-1160 27 Ogawa R The importance of adipose- derived stem cells and vascularized tissue regeneration in the field of tissue transplantation Curr Stem Cell ... transplantation of human adipose tissue- derived stromal cells after cerebral ischemia in rats Exp Neurol 2003, 183, 355-366 Kang SK, Shin MJ, Jung JS, Kim YG, Kim CH Autologous adipose tissue- derived ... Immunophenotype of human adiposederived cells: temporal changes in stromal-associated and stem cell-associated markers Stem Cells 2006, 24, 376-385 26 Muir WW 3rd, Wiese AJ, March PA Effects of morphine,...

Ngày tải lên: 07/08/2014, 23:22

... approximately 40 cm3 of packed adipose tissue, fibrous tissue, red blood cells and a small number of nucleated cells An equal volume of digestive enzyme, 0.07% collagenase type 1, composed of several collagenases, ... of human bones in hip osteonecrosis and human cartilage in knee osteoarthritis with autologous adipose- tissue- derived stem cells: a case series Journal of Medical Case Reports 2011 5:296 Submit ... Mesenchymal stem cell therapy in joint disease Novartis Found Symp 2003, 249:86-96 Zhang HN, Li L, Leng P, Wang YZ, Lv CY: Uninduced adipose- derived stem cells repair the defect of full-thickness...

Ngày tải lên: 10/08/2014, 23:21

... dilutions of DCs, from 10,000 cells per well to 313 cells per well, with × 105 allogeneic CD4 T cells in a 96-well U-bottomed plate in a total volume of 200 µl for days The proliferation of T cells ... expression profile of DCs, we suspected that LV infection might also affect DC activation of T cells To test this, we set up an in vitro immunity assay using co-culture of human DCs and naïve T cells ... function of DCs Polarization of naïve Th cells into Th1 cells is critical for the induction of cellular immunity against intracellular pathogens and cancer cells The observed impairment of the...

Ngày tải lên: 13/08/2014, 13:20

... Detection of smooth muscle cells after differentiation of the BMP2+ cells Figure Detection of smooth muscle cells after differentiation of the BMP2+ cells (a) Expression of SMA in the BMP2+ cells ... expression (e) Figure Differentiation of the BMP2+ cells to cardiac cells Differentiation of the BMP2+ cells to cardiac cells (a) RT-PCR analysis of the cardiac markers in BMP2+ cells and other controls ... BMP2-expressing cells during early differentiation of ES cells will pave the way for a better understanding of NCSCs and their differentiation into lineages The BMP2+ cells derived from the ES cells may...

Ngày tải lên: 14/08/2014, 08:20

... cells [31,32] and other cells of the innate immune system (for example, natural killer cells) , as well as in exerting Genome Biology 2007, 8:R30 information Our finding of negligible levels of ... Figure Phenotypic comparison of cells Phenotypic comparison of cells The expression of cell surface markers on (a) immature and (b) mature mDCs, moDCs and KG1 cells Immature cells were freshly isolated ... model systems, moDCs and KG-1 cells, our findings identified important differences between the proteomes of these cells, and the findings were confirmed by ELISA detection of a selection of proteins...

Ngày tải lên: 14/08/2014, 18:20

... pluripotent stem cell derived -neural stem cell human induced pluripotent stem cell derived -neural stem cell expressing CodA human induced pluripotent stem cell derived -neural stem cell expressing Fcy human ... 1.3.5 Current status of gene therapy for glioma and breast cancer 22 1.4 Stem cells 1.4.1 Adult stem cells 1.4.1.1 Neural stem cells 1.4.2 Induced pluripotent stem cells 1.5 Stem cell as a vehicle ... in section 3.1) 1.4 Stem cells 1.4.1 Adult stem cells (ASCs) Adult stem cells (ASCs), also known as somatic stem cells, are multipotent undifferentiated stem cells found in a tissue or organ that...

Ngày tải lên: 09/09/2015, 18:56

... in human neuroepithelial-like stem cells for the generation of modified neuronal cultures 59   4.2   Generation of gene-corrected neural stem cells from MJD patient -derived iPS cells ... that human neural stem cells (lt-NES cells) , being precursors of glia und neurons were also suitable candidates for transduction 1.3.3 Alternative systems for genetic modifications of human cells ... Introduction 1.1 Stem cells Stem cells have the remarkable potential to differentiate into specialized cells and thereby are key factors for the development of the whole organism All types of stem cells...

Ngày tải lên: 26/11/2015, 09:54