a thread safe counter is achieved by synchronizing class methods
Báo cáo khoa học: Kinetics of violaxanthin de-epoxidation by violaxanthin de-epoxidase, a xanthophyll cycle enzyme, is regulated by membrane fluidity in model lipid bilayers pptx
... which all molecules of violaxanthin are converted to antheraxanthin and all molecules of antheraxanthin are converted to zeaxanthin at a given temperature at saturating amount of VDE and at the ... violaxanthin to antheraxanthin than on conversion of antheraxanthin to zeaxanthin (Table 1) At low monogalactosyldiacylglycerol concentration (5 mol%), probability of violaxanthin to antheraxanthin ... violaxanthin was deepoxidated and more zeaxanthin was formed in the monogalactosyldiacylglycerol system at the plateau stage of the reaction The same was found at °C, although at this temperature...
Ngày tải lên: 08/03/2014, 10:20
Báo cáo khoa học: Induction of PAI-1 expression by tumor necrosis factor a in endothelial cells is mediated by its responsive element located in the 4G/5G site ppt
... et al After 48 h cells were treated with TNFa (50 ngÆmL)1) and total cellular RNA isolated h later by using the TRIzol Reagent Statistical analysis All values are expressed as mean ± SE compared ... using a Quick Change site-directed mutagenesis kit (Stratagene, La Jolla, CA, USA) with mutagenic primers: 5¢-ACATGGGGGAGTCAGCC-3, 5¢-GGCTGACTCC CCCATGT-3¢; 5¢-ACATGGGGCAGTCAGCC-3, 5¢-GG CTGACTGCCCCATGT-3¢; ... at least times with reproducible results and data are shown as mean ± SD (E, F) Changes in PAI-1 expression as measured at the level of PAI-1 promoter activity and PAI-1 mRNA evaluated by real-time...
Ngày tải lên: 30/03/2014, 11:20
Tài liệu Báo cáo khoa học: TMPRSS13, a type II transmembrane serine protease, is inhibited by hepatocyte growth factor activator inhibitor type 1 and activates pro-hepatocyte growth factor pdf
... TMPRSS13 mRNA is expressed in a variety of human adult tissues, and predominantly in lung, placenta, pancreas and prostate [12] HAI-1 mRNA is also highly expressed in placenta, pancreas and prostate ... D, Takahashi K, Okigaki M, Arakaki N, Nakayama H, Hirono S, Sakiyama O, Takahashi K et al (1989) Molecular cloning and sequence analysis of cDNA for human hepatocyte growth factor Biochem Biophys ... 5¢-GGATTTTCTGAATCGCACCT-3¢ for TMPRSS13 (34 cycles), and 5¢-ATGGAGGCTGCTTGG GCAACA-3¢ and 5¢-ACAGGCAGCCTCGTCGGAGG-3¢ for HAI-1 (26 cycles) The GAPDH-specific primer set, 5¢-AGGTGAAGGTCGGAGTCAAC-3¢ and...
Ngày tải lên: 15/02/2014, 01:20
Tài liệu Báo cáo khoa học: Transcription of individual tRNA1Gly genes from within a multigene family is regulated by transcription factor TFIIIB pdf
... was analysed by electrophoresis on 4% polyacylamide (nondenaturing) gels and visualized in a Phosphorimager Lanes as marked The heparin-resistant complex on tRNA1Gly -1 (left) is marked by an ... FEBS A Parthasarthy and K P Gopinathan completely abolished when the TATATAA sequence was mutated to GATATCA (lanes 3, 4) These results were also consistent with the observation that TFIIIB alone ... Regulation of pol III transcription A Parthasarthy and K P Gopinathan and making them unavailable for transcription The ‘TATATAA’ sequence present in the flanking regions Gly of tRNA1 -6,7 was responsible...
Ngày tải lên: 20/02/2014, 02:21
Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
... d(5¢-AACAACGCAGCTGGGCTCTG GAACCAT), ECF -A1 41Q d(5¢-TCAACCTCTAACCAG GCTACTCCGCTG) ECM-G77Q d(5¢-AACAACGCTGG CCAGCACGCTAACCAC) and ECM-Q14 6A d(5¢-TCT ACTGCTAACGCGGATTCTCCGCTG) following the manufacturer’s ... human manganese superoxide dismutase: effects on structure, stability, and catalysis Biochemistry 39, 7131– 7137 31 Hiraoka, B.Y., Yamakura, F., Sugio, S & Nakayama, K (2000) A change of the metal-specific ... Institut Jacques Monod, Paris, France) as template DNA MnSOD was cloned using the primers ECM-5¢ d(5¢-AGCTATACCCTGCCATCCCTG) and ECM-3¢ d(5¢-TTATTTTTTCGCCGCAAAACGTG) and E coli genomic DNA as template...
Ngày tải lên: 21/02/2014, 01:21
Báo cáo khoa học: Expression of the Drosophila melanogaster ATP synthase a subunit gene is regulated by a transcriptional element containing GAF and Adf-1 binding sites pptx
... 365–377 Biswas, G., Adebanjo, O .A. , Freedman, B.D., Anandatheerthavarada, H.K., Vijayasarathy, C., Zaidi, M., Kotlikoff, M & Avadhani, N.G (1999) Retrograde Ca2+ signaling in C2C12 skeletal myocytes ... by PCR from total DNA using the primers pADm1 (forward; 5¢-AGCAGTCGACGA AGCGACGAAGTGAAGCTGCGTGA-3¢) and pADm3 (reverse; 5¢-ATCCGTCGACATGCTTTTTAACTGTT CG-3¢) After digestion with SalI (which recognizes ... differentiated, both structurally and functionally, with highly developed cristae full of ATP synthase complexes For this reason, ATP synthase and in particular the a- F1-ATPase and b-F1-ATPase catalytic...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: Tumor necrosis factor-a converting enzyme is processed by proprotein-convertases to its mature form which is degraded upon phorbol ester stimulation pptx
... following antibodies were used: anti-ADAM10, a polyclonal rabbit antibody against endogenous ADAM10 and anti-TACE, and a polyclonal rabbit antibody against endogenous TACE (Chemikon International, ... substrate CDPstar (Tropix) Emitted light was densitometrically analyzed by using a digital camera and the software AIDA 2.0 (Raytest, Straubenhardt, Germany) Isolation and detection of APPsa by ... 25–31 Takahashi, S., Kasai, K., Hatsuzawa, K., Kitamura, N., Misumi, Y., Ikehara, Y., Murakami, K & Nakayama, K (1993) A mutation of furin causes the lack of precursor-processing activity in human...
Ngày tải lên: 08/03/2014, 02:20
Báo cáo Y học: Proteasome-driven turnover of tryptophan hydroxylase is triggered by phosphorylation in RBL2H3 cells, a serotonin producing mast cell line pptx
... Ugai, S., Tamura, T., Tanahashi, N., Takai, S., Komi, N., Chung, C.H., Tanaka, K & Ichihara, A (1993) Purification and characterization of the 26S proteasome complex catalyzing ATPdependent breakdown ... Comparisons of tryptophan hydroxylase from a malignant murine mast cell tumor and rat mesencephalic tegmentum Arch Biochem Biophys 199, 355–361 13 Hasegawa, H., Yanagisawa, M., Inoue, F., Yanaihara, ... SDS/PAGE for autoradiography as described in Materials and methods The autoradiogram (left panel, 32P) represents treatment with vehicle (lane 1), 0.5 lM okadaic acid (lane 2), and okadaic acid...
Ngày tải lên: 08/03/2014, 16:20
Báo cáo khoa học: PCSK9 is phosphorylated by a Golgi casein kinase-like kinase ex vivo and circulates as a phosphoprotein in humans doc
... the prodomain, the common naturally occurring A5 3V variation that has no significant effect on plasma cholesterol levels and the R46L variation, a naturally occurring variant associated with hypocholesterolemia ... (Burlington, Canada) and the goat anti-(C-terminal PCSK9) IgG used for immunoblotting from Imgenex (San Diego, CA, USA) Secondary anti-mouse and anti- (rabbit HRP) IgG were from Amersham (Piscataway, NJ, ... in media containing 32P-orthophosphate, immunoprecipitated PCSK9 from cells and media, and analyzed samples by SDS ⁄ PAGE fractionation followed by phosphorimaging (Fig 5) Lanes and and and represent...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: In vitro gamma-secretase cleavage of the Alzheimer’s amyloid precursor protein correlates to a subset of presenilin complexes and is inhibited by zinc potx
... arrow marked ‘c std’ Lane 2, lysate sample alone Note that a separation between c-3FLAG standard and e-CTF-3FLAG was achieved in lane An uncharacterized anti-FLAG immunoreactive protein migrating ... e-CTF-3FLAG band was greater in lane spiked with e-3FLAG standard (C) A similar experiment to that in (B) was performed except that lane is a sample spiked with c-3FLAG standard indicated by the arrow ... 5¢-GGGGGGCCAT GGCGACAGTGATCGTC-3¢; reverse, 3FLAG HindIII creating the plasmid c-3FLAG standard Finally, the primer pairs forward, 5¢-GGGGGGCCATGGTGATGCTGA AGAAGAACAG-3¢ and reverse 3FLAG HindIII were...
Ngày tải lên: 16/03/2014, 23:20
Báo cáo Y học: Balanced expression of single subunits in a multisubunit protein, achieved by cell fusion of individual transfectants docx
... The absorbance was read by TitertekÒ Multiskan (ICN Flow, USA) The amount of IgA present in each supernatant was calculated relative to a standard preparation with known concentration Verification ... Kodak X-OMAT film for 15–60 s Dot blot density was analysed by TOTALLAB gel software (Phonetix, UK) The amount of SC present in each supernatant was calculated relative to a standard preparation ... cell, an IgA and SC expression level analysis was done Whereas SC is readily secreted without assembly with the other components, this is not the case for a1 heavy-chain and J-chain Both are retained...
Ngày tải lên: 18/03/2014, 01:20
Hanging by a Thread potx
... in his life, Captain Atef Abdullah Al-Amin allowed his voice to betray him Arabic is normally spoken about half an octave above the normal tone used for English And, unlike American English, ... then, what's the difference between a dictator and a true prophet? So was he Randall Garrett The Asses of Balaam The remarkable characteristic of Balaam's ass was that it was more perceptive than ... was beating one of his favorite drums, and he went on with his tirade in a fine flush of fury Alas … poor Jayjay Actually, Jayjay Kelvin can't be blamed for his attitude All he was saying was...
Ngày tải lên: 23/03/2014, 00:20
Báo cáo khóa học: Emerin binding to Btf, a death-promoting transcriptional repressor, is disrupted by a missense mutation that causes Emery–Dreifuss muscular dystrophy pdf
... 45AAA 45EEE 104AYGEAEA 122AA 145AA 151AA 161AAA 175AAA 192ASAAA 198AAAAA 206AAPA 24AAAA 34AAAA 54F 70AAAMA 76APAKADAA 112AASRAVAAAVA 133H 141AASAEECKAA 164AAHARPA 179AA 183H 196AA 207AA 214AAGA ... DNA sequence of the primer AAC ATA TGG ATC AGG AAG CTC TAG ATT AC AAC ATA TGG CAC GAG AAA AGT CTA CCT TC AAC ATA TGA TCA CGC ACT ACC GCC C TCC ATA TGC TAG AAG GGG TTG CCT GGC GGA TCC CTA GAA ... GGG GAT CCC TGG CCC CAG AGC GG TTG GAT CCT TAT GTA CTA GCA AGC AGC C TTG GAT CCT TAT TGC CGA GTA CTA TGT TC TTG GAT CCT TAG GGA GAA GAA GGT GAT G AAA GAT CTT TAT TCC TTT TCT TCC TTG CG temperature...
Ngày tải lên: 23/03/2014, 12:20
Báo cáo khoa học: The activity of Plasmodium falciparum arginase is mediated by a novel inter-monomer salt-bridge between Glu295–Arg404 doc
... are distinguished by different shades of blue The image was generated using PYMOL dues in the bacterial and mammalian templates P falciparum Glu295 aligns with an Asp in mammals (human arginase ... amidohydrolase [17] Agmatine is formed by decarboxylation of arginine (arginine decarboxylase) and is converted by agmatinase to putrescine and urea Arginase is thus part of one of two alternative biosynthetic ... sickness, Chagas’ disease, and leishmaniasis Amino Acids 33, 359–366 12 Wallace HM (2007) Targeting polyamine metabolism: a viable therapeutic ⁄ preventative solution for cancer? Expert Opin Pharmacother...
Ngày tải lên: 29/03/2014, 23:20
Báo cáo khoa học: Transcription of mammalian cytochrome c oxidase subunit IV-2 is controlled by a novel conserved oxygen responsive element pptx
... GCTGAAGACCGCGGAGGTAC GAGGTACCCAGAACTGCCCTG GATAGTCAGTGGGGGAAACCTCAG CAGCAAAAGAGGGCTGTGTGGTG TGGCCGCCACGAACATCCCATC GTTGCCCAGGTTGGAGTGCAG CTCGCGGGCTCGGCAGTGGGAG AGTCTATTCTCGAGCACCTGGGACTACAGG AGTCTATTCTCGAGCCCAAAGCGCTGAGATTACAG ... AGTCTATTCTCGAGCCCAAAGCGCTGAGATTACAG AGTCTATTCTCGAGATGCTTCTGGAGTAGGAGGCA AGTCTATTCTCGAGGTGTGGAGGAGGCAGGGAGAC AGTCTATTCTCGAGGAGGCGCTCTGCAGTGCCTC AGTCTATTCTCGAGAAGCAGGACGTTCCCACGCTG AGTCTATTCTCGAGGGGGCGGGCGCCCGCACTCAG ... ATATTCTAGGATCCTGGCTCATTCACTGCTGTCAC ATATTCTAGGATCCCGGCTTCCCCCTCCCTGCAG GCAAATTCTTACTGAGCTTTTACTATATGCACAGC GGACGTTCCCACGCTGGGG GGTCGTAACCACGCTGGGG GGCTGTGGGGCGGGCCGG GGCTGTGGGTATGGCCGG TTCGATCGGGGCGGGGCGA...
Ngày tải lên: 30/03/2014, 03:20
Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot
... indicate that calpain is activated in both tissues, although at a higher rate in aorta Further direct evidence in support of calpain activation in aorta was provided by the degradation of talin and ... The Authors Journal compilation ª 2008 FEBS 2503 In vivo degradation of NOS and HSP90 by calpain M Averna et al Table Levels of native and 15 kDa calpastatin species in brain and aorta of NMS and ... [28], and studied calpain degradation of NOS and HSP90 in brain and aorta To amplify the range of fluctuations in [Ca2+]i, hypertensive Milan strain (HMS) rats were also used, as they are characterized...
Ngày tải lên: 30/03/2014, 04:20
Báo cáo khoa học: A novel glycogen-targeting subunit of protein phosphatase 1 that is regulated by insulin and shows differential tissue distribution in humans and rodents pdf
... QLQRDALRHFAPCPPRARGLQEARVALEPALEPGFAARLQAQRICLERADAGPLGVAGS RAT R3E 119 QLQRDALRHFAPCPPRTRGLQDARIALEPALEPGFAARLQAQRICLERADAGPLGVAGS HUMAN R3E 119 QLQRDALRHFAPCQPRARGLQEARAALEPASEPGFAARLLTQRICLERAEAGPLGVAGS PP1 ... ARVLDLAYEKRVSVRWSADGWRSLRESPASYAGPAPSPPRADRFAFRLPAPPVGGTLLF RAT R3E 178 ARVLDLAYEKRVSVRWSADGWRSLRESPASYAGPAPAPPRADRFAFRLPAPPVGGALLF HUMAN R3E 178 ARVVDLAYEKRVSVRWSADGWRSQREAPAAYAGPAPPPPRADRFAFRLPAPPIGGALLF GM/ RGL/R 3A ... gacgaggcgcctgcggccgacggcggaaaacaccaaaggcacccgggggcggggcgacccgatgtggcggggaggagtag 920 I H F I * 279 921 gagagaccaggattggcgggagcggtccaagggagtc 957 Fig (A) Diagram of human PPP1R3E mRNA compared with PPP1R3E...
Ngày tải lên: 30/03/2014, 16:20
Báo cáo khoa học: Inactivation of phosphorylase is a major component of the mechanism by which insulin stimulates hepatic glycogen synthesis doc
... preparations indicated Statistical analysis was by Student’s paired t test Fig Time course of inactivation of phosphorylase a (A) and activation of glycogen synthase (B) by 10 nM insulin Hepatocytes ... phosphatase This reverses the inhibition of synthase phosphatase by phosphorylase a There is a long-standing debate as to whether inactivation of phosphorylase is a component of the mechanism by ... that the stimulation of glycogen synthesis by inactivation of phosphorylase is associated with both activation and translocation of glycogen synthase, and that the former mechanism alone cannot...
Ngày tải lên: 31/03/2014, 01:20
Báo cáo khoa học: Activated transglutaminase from Streptomyces mobaraensis is processed by a tripeptidyl aminopeptidase in the final step pptx
... Gly-Arg-pNA Ala-Ala-Pro-pNA Ala-Phe-Pro-pNA Ala-Ala-Ala-pNA Pro-Leu-Gly-pNA Ala-Ala-Phe-pNA Suc-Ala-Ala-Phe-pNA Val-Leu-Lys-pNA Cbz-Pro-Phe-Arg-pNA Ala-Ala-Val-Ala-pNA Ala-Ala-Pro-Leu-pNA Suc-Ala-Ala-Pro-Phe-pNA ... Substrates Activity (nmolÆmin)1Æml)1) Relative activity (%) Pro-pNA Phe-pNA Leu-pNA Ala-pNA Ala-Pro-pNA Suc-Ala-Pro-pNA Gly-Pro-pNA Cbz-Gly-Pro-pNA Ala-Ala-pNA Ala-Phe-pNA Gly-Glu-pNA Gly-Arg-pNA Ala-Ala-Pro-pNA ... hydrolyse Ala-Ala-pNA and Ala-pNA (or other chromogenic amino acids) at reasonable rates clearly indicates exclusive cleavage of the anilide bond of Ala-Ala-Val-Ala-pNA Our results also provide...
Ngày tải lên: 31/03/2014, 07:20
Báo cáo sinh học: " Panicovirus accumulation is governed by two membrane-associated proteins with a newly identified conserved motif that contributes to pathogenicity" pptx
... CAGCCAACTGGGCAGCCTCTGTG CTCCAGACAGCCGCCTGGTTAGC CACACCCTGTAGAGGGCTCTCCAG CCTTTCTTATCAGCCACCCTGTAGAG GGAATACAGCTGGCAAGGC TGATCCTGGGCGTATGCGC GCCCCAACTAATGCATTGGTCACTAG CCAAGCAGTCGCATTGGCCCC a The altered nucleotides ... plants CD mutations not affect RNA cis-elements Many RNA viruses contain RNA cis-elements that can affect replication To test if amino acid changes (F31 3A, F35 7A, and W405) may have inactivated ... peroxidase (Amersham Pharmacia Biotech, Piscataway, NJ) was used at a 1:5,000 dilution and assayed by enzymatic reactions The remaining half of the extract was prepared for RNA blots, as described...
Ngày tải lên: 19/06/2014, 08:20