5 apos end of expressed genes in zebrafish

... CG5001 CG5139 CG5011 CG14342 CG14343 CG5 156 CG5397 CG5423 CG14344 CG143 45 CG5430 CG5440 CG5 450 CG 555 6 CG 556 1 CG 556 4 CG 556 5 CG16933 CG14346 CG 557 4 CG4947 CG5001 CG5139 CG5011 CG14342 CG14343 CG5 156 ... CG14343 CG5 156 CG5397 CG5423 CG14344 CG143 45 a5 CG5440 Cdlc2 CG 555 6 CG 556 1 CG 556 4 CG 556 5 NLaz CG14346 CG 557 4 CT 354 52 CT381 65 CT10673 CT10 659 CT10601 CT27262 CT106 15 CT27264 CT12 153 CT42330 CT12137 ... Spellman and Rubin (b) http://jbiol.com/content/1/1 /5 (c) CT 158 82 CT 158 84 CT16 455 CT16096 CT339 75 CT33976 CT1 650 3 CT1 652 7 CT17 158 CT33977 CT33978 CT17230 CT17 252 CT17290 CT1 755 8 CT1 755 4 CT17492 CT17332...

Ngày tải lên: 06/08/2014, 18:20

... 0.6 0.4 0.2 2 .5 1 .5 acgh 0 .5 5' chr20 47 950 000 3' 47 955 000 GSDMB 25 20 15 coverage 10 acgh chr20 380 650 00 3' coverage 5' BCAS4 40 30 20 10 acgh 380 750 00 38070000 chr20 5' 49430000 49 450 000 49470000 ... multiple lines of evidence suggesting the potential functional importance of the fusion genes, including the involvement of known oncogenic partner genes, exclusive expression of the partner genes ... expression of the exons of the 3’ partner genes taking part in the fusions Exonic expression of CEP 250 in ZMYND8-CEP 250 (upper), ENSG00000236127 in CSE1LENSG00000236127 (second from top), GSDMB in TATDN1-GSDMB...

Ngày tải lên: 09/08/2014, 22:23

... find that the voting method again improves the results, returning very similar numbers of correct genes as a 5v5 regularized t-test (Figure 5) The voting method returns 41% more of the top 50 ... 2004, 5: R70 GEO/R.t-test vote 2v2 Regularized t-test 2v2 GEO 2v2 t-test 25 20 15 10 50 100 150 200 Number of genes 250 300 15 10 250 300 Figure dystrophy dataset A comparison of each method in 1v1 ... value of the higher rank A final score was obtained by combining the results from each method in this way, and the genes were re-ranked on the basis of this score By using the 75% / 25% ratio, genes...

Ngày tải lên: 14/08/2014, 14:21

... 1401-1 450 1 451 - 150 0 1401-1 450 1 451 - 150 0 1401-1 450 1 451 - 150 0 951 -1000 901- 950 851 -900 801- 850 751 -800 701- 750 651 -700 601- 650 55 1-600 50 1 -55 0 451 -50 0 401- 450 351 -400 301- 350 251 -300 201- 250 151 -200 ... 801- 850 751 -800 701- 750 651 -700 601- 650 55 1-600 50 1 -55 0 451 -50 0 401- 450 351 -400 301- 350 251 -300 201- 250 151 -200 101- 150 che-2::PABP/acr -5: :PABP ranks 1001-1 050 951 -1000 901- 950 851 -900 801- 850 751 -800 ... 751 -800 701- 750 651 -700 601- 650 55 1-600 50 1 -55 0 1 -50 51 -100 Fraction Fraction Volume 6, Issue 2, Article R17 451 -50 0 401- 450 351 -400 301- 350 251 -300 201- 250 151 -200 101- 150 51 -100 1 -50 Fraction...

Ngày tải lên: 14/08/2014, 14:21

... Gli2 In addition, both N-terminal repressor domain and C-terminal activator domain containing VP16-like 153 Discussion TAF binding domain and CBP binding site are found in Gli2b, indicating a ... role of gli2/gli2b in development of the zebrafish brain, especially the hindbrain Our results indicated that Gli2b plays a more important role in regulating Hh signaling in zebrafish hindbrain ... 0. 15 100 12(12%) 28(28%) 54 (54 %) 6(6%) 0.3 100 15( 15% ) 13(13%) 49(49%) 23(23%) 0.6 50 7(14%) 5( 10%) 17(34%) 21(42%) 100 35( 35% ) - 11(11%) 54 (54 %) gli2bMMA 50 3(6%) 45( 94%) 2(4%) - gli2bSpl MO Injected...

Ngày tải lên: 15/09/2015, 17:10

... alignment of mouse Gli2 proteins indicated that the sequence similarity is high in the N-terminal, including the five zinc-finger domains and six putative Protein Kinase A binding domains However, ... expression in the hindbrain acquires a characteristic pattern at 32 hpf in the dorsal hindbrain (Fig.3-9A); it increased in the bilateral domains in the lateral hindbrain at the level of rhombomere ... gli2b is not affected in both medial and lateral domains of the hindbrain Thus, FGF signaling seems not involved in regulation of gli2b expression in the midbrain and hindbrain 112 Results A B C...

Ngày tải lên: 15/09/2015, 17:10

... striping 2.3 Protein applications 2.3.1 in vitro coupled transcription and translation 2.3.2 SDS-polyacrylamide gel electrophoresis (PAGE) 2.4 Expression Analysis 54 54 54 55 55 56 56 57 57 57 57 ... 57 58 58 58 58 58 59 59 59 60 60 61 62 62 63 64 64 64 65 65 65 66 67 67 68 69 70 71 71 71 71 71 72 72 72 73 73 73 73 74 III Contents 2.4.1 Zebrafish 2.4.1.1 Fish maintenance 2.4.1.2 Mutant lines ... differentiation 3 .5. 5 Gli2b and axonogenesis 3 .5. 6 Gli2b and formation of axonal scaffold 114 114 119 122 123 1 25 129 Chapter IV Discussion 150 153 154 155 4.1 Zebrafish Gli2b belong to Ci/Gli zinc finger...

Ngày tải lên: 15/09/2015, 17:10

... DNA-binding domain and a less conserved C-terminal protein-binding domain A comparison of the protein sequences of IRF6 in human, mouse, Xenopus, zebrafish and Fugu reveals that their DNA-binding ... truncated protein in the presence of binding reaction mixture (1X binding buffer; 2 .5% glycerol; mM MgCl2 ;50 ng/ul bovine serum albumin and 50 ng/ul poly(dI:dI)-poly(dI:dC)] The DNA-protein complexes ... analysis of the skin showed over-proliferation in the spinous layer, and failure of termination of cell differentiation, contributing to the abnormal skin (Ingraham et al., 2006) This finding suggests...

Ngày tải lên: 01/10/2015, 17:26

... DNA-binding domain and a less conserved C-terminal protein-binding domain A comparison of the protein sequences of IRF6 in human, mouse, Xenopus, zebrafish and Fugu reveals that their DNA-binding ... truncated protein in the presence of binding reaction mixture (1X binding buffer; 2 .5% glycerol; mM MgCl2 ;50 ng/ul bovine serum albumin and 50 ng/ul poly(dI:dI)-poly(dI:dC)] The DNA-protein complexes ... analysis of the skin showed over-proliferation in the spinous layer, and failure of termination of cell differentiation, contributing to the abnormal skin (Ingraham et al., 2006) This finding suggests...

Ngày tải lên: 09/10/2015, 10:49

... subject of much business abuse, both in terms of the number of meetings held and in terms of what business people think of most meetings Indeed, business people often chafe when the subject of committee ... Airline workers took an average pay cut of 15 percent for 55 percent interest in the company and of its 12 seats on the airlines board of directors According to Business Week, worker ownership of ... During the winter of 1973-74, the United States was in the midst of an “energy crisis.” Prices of gasoline and other fuels were being held down in spite of the limited imports of fuel coming into...

Ngày tải lên: 17/12/2013, 15:18

... Analysis of the N-terminal repeat domain of zebrafish PrP1 and PrP2 The N-terminal domain of zebrafish PrP1 contained nine repeats (residues 53 –332) including four highly conserved 37 amino acid-long ... transcripts in hair cells (hc), but not in supporting cells (sc) Scale bars ¼ 100 lm in A–R, and lm in S 50 8 FEBS Journal 272 (20 05) 50 0 51 3 ª 2004 FEBS E Cotto et al Expression of prion genes in the ... last finding should be of interest because in terms of function, the fish intestine is highly regionalized both in the larva and in the adult [38] The posterior segment of the fish intestine is the...

Ngày tải lên: 19/02/2014, 16:20

... Translation Proceedings of the 11th Conference of the European Association for Machine Translation, Oslo Henderson, J 2004 Discriminative training of a neural network statistical parser In ACL 2004, ... of subtrees of all trees increases with the Catalan number, and only ad hoc sampling could make the method work Since U-DOP* computes the shortest derivations (in the training phase) by combining ... number of subtrees in the shortest derivations is linear in the number of nodes (see Zollmann and Sima’an 20 05, theorem 5. 2) This means that UDOP* results in an STSG which is much more succinct...

Ngày tải lên: 20/02/2014, 12:20

... hydrogenase genes [ 25] In Rhodopseudomonas palustris CGA009, the photosystem is synthesized in the dark due to a single point mutation in the helix–turn–helix DNA binding motif of PpsR, rendering it inactive ... used in the following concentrations (lgÆmL)1): for E coli: streptomycin (50 ), ampicillin (100), kanamycin (50 ), gentamycin (20), erythromycin (50 ); for T roseopersicina: streptomycin (5) , kanamycin ... role of the N-terminal part of the kinase, containing a PAS domain, was established in signal transduction between the RH and the kinase [6,7] Addition of H2 to HupUV before or during the incubation...

Ngày tải lên: 16/03/2014, 23:20

... amount of protein needed in combination with the intended use (in vitro, in vivo, structural biology) The design of a suitable expression vector for an expression system of choice, also including ... myeloma cell lines for recombinant protein production are J 558 L [secreting immunoglobulin (Ig) Zl light chains19], nonsecreting Sp2/0 Agl42°,21 and NSO cells.22-24 For expression of engineered chimeric ... as neomycin or hygromycin, positive transfectants can be selected in media containing the corresponding antibiotic Induction of gene amplification using glutamine synthetase22 or dihydrofolate...

Ngày tải lên: 11/04/2014, 09:41

... studying the influence of SARS- CoV on the immune system-independent induction of cytokines Interferon genes and their antiviral effectors To properly assess the cytokine profile of SARS-CoV infection, ... production of chemokines by the infected tissue cells Results Virus-induced cytokines not only play a significant role in host defense, but also in immunopathogenesis Investigations of the cytokine profiles ... dissemination of SARS-CoV By slowing down expression of IFNs and their antiviral genes in the infected tissue cells, the virus buys time during the initial, critical phase of infection in order...

Ngày tải lên: 20/06/2014, 01:20

... following by 35 cycles of 940C for min, 61 0C for min, 72 0C for min; and 720C for for final extension 12 2.2.3 PCR analysis to detect the CaMV 35S promoter The primer pair, forward primer 5' GACCTAACAGAACTCGCCGTA-3' ... thaliana AOS proteins localize on chloroplast (CP), microbody (MB), mitochondria (MC) In order to increase the level of endogenous JA in rice, we try to over express AOS genes by transforming AtAOS2 ... primer 5' CACTTGCTTTGAAGACGTGGT-3', was designed from the sequence of CaMV35S promoter which located infront of AtAOS2 gene in pCAMBIA1201/AtAOS binary vector (Fig 3) PCR was carried out according...

Ngày tải lên: 06/08/2014, 18:21

... layer protein) Fibronectin PRELP (proline/arginine-rich and leucine-rich repeat protein) Chondroadherin Fibrillin Tenascin-C Elastin MGP (matrix Gla protein) Chondromodulin-I Chondromodulin-II CD-RAP ... changes in joints J Clin Invest 1993, 92 :58 2 -59 5 51 Rintala M, Metsaranta M, Garofalo S, de Crombrugghe B, Vuorio E, Ronning O: Abnormal craniofacial morphology and cartilage structure in transgenic ... [23], linkage analysis of 14 candidate genes in OA kindreds resulted in the exclusion of 10 important cartilage genes, including COL2A1 [40] Analysis of 47 families with the phenotype of earlyonset...

Ngày tải lên: 09/08/2014, 06:22

... AJ30 957 1) from the BAC clone 55 5-N16 (Research Genetics Inc., USA), which contains 1 05 kb of the murine casein locus [8] A ∼ 24-kb BamHI fragment from this clone, containing the complete κ-casein ... the corresponding regions in the ruminant κ-casein flanking sequences MATERIALS AND METHODS 2.1 Origin of sequences The murine sequence was generated from a subclone of BAC clone 55 5-N16 (Research ... micelle size [ 15] , which in turn alters the manufacturing properties and digestibility of milk [5] In spite of the importance of κ-casein in the assembly and stability of casein micelles, a detailed...

Ngày tải lên: 09/08/2014, 18:21

... Higher H3K9ac on inducible genes is independent of the presence of CpG islands Higher H3K9ac on inducible genes is independent of the presence of CpG islands (a) The percentage of genes with CpG ... transcripts of the ternary complex provide insight into RNA polymerase II elongational pausing J Mol Biol 19 95, 252 :52 2 -53 5 Law A, Hirayoshi K, O'Brien T, Lis JT: Direct cloning of DNA that interacts in ... others of nucleosome depletion [27] on inducible genes in non-stimulated cells suggests that the promoters of these genes resemble those of active genes regardless of the level of mature mRNA in...

Ngày tải lên: 09/08/2014, 20:20

... documented role in the local accumulation of monocytes [8,9] However, fenofibrate had an intriguing effect in SDF-1 expression neutralizing the significant decrease in its expression observed in untreated ... significant effect of fenofibrate in the MCP-1/CCL2 gene expression However, there was a significant decrease in the gene expression of the CX3CL1/ Fractalkine in patients treated with fenofibrate with ... significant decreases in the expression in circulating leukocytes of CX3CL1 and CCR2 genes and a significant increase in the expression of SDF-1 Page of (page number not for citation purposes)...

Ngày tải lên: 10/08/2014, 05:21