4 stem cells in the epidermis and hair follicle

... domain to a new DNA-binding domain (Kim et al., 1996) The first DNA-binding domain used was a fruit fly homeobox domain, followed by zinc-finger DNA binding domain and yeast Gal4 DNA-binding ... modify the DNA-binding domain to bind specifically chosen target sequences, allowing an induced double strand break at a defined position Zinc finger modules are part of the DNA-binding domain of ... system of adenosine-releasing fibroblasts with inactivated adenosine kinase and adenosine deaminase These cells were transplanted into brain ventricles in a rat epilepsy model and were shown to...

Ngày tải lên: 26/11/2015, 09:54

... line with the induction of Tregs in vitro and in vivo in our study Moreover, in our experiments we did not find UC-MSCs in the joint of the CIA mice The short-term presence of UC-MSCs in the spleen ... CIA in mice UC-MSCs downregulated the production of the proinflammatory cytokines TNF-a, and IL-6 in vitro and in vivo In addition, MCP-1 is a member of the CC family and could be induced by inflammatory ... were added in the lower well of the invasion plate, with FLSs (150,000) alone in the well inserts Forty-eight hours later, the inserts were stained with the cell stain solution and the OD 560...

Ngày tải lên: 12/08/2014, 15:22

... germ cells and ES cells is their epigenetic imprint status Shinohara reported that while the spermatagonial cells retain a stable androgenic imprint over long-term culture, the pluripotent cells ... into an oocyte or fused with an ES cell, the underlying mechanisms remain obscure Using the ES fusion system, Smith has found an unexpected role for the homeodomain-containing protein Nanog in ... by our inability to derive rat ES cells Shinohara hinted that he was making progress in applying his findings to the rat Finally, Tariq Enver (University of Oxford, UK) described a mathematical...

Ngày tải lên: 14/08/2014, 16:21

... of chemotherapeutic agents, including anthracyclines (e.g daunorubicin and doxorubicin) [4], Vinca alkaloids (vincristine and vinblastine) [5], paclitaxel [6], and camptothecins [7] In addition ... product drugs, including etoposide, Vinca alkaloids (vincristine and vinblastine), anthracyclines (e.g daunorubicin and doxorubicin) [36], and camptothecins [7] It seems that MRP1/ABCC1 and MRP2/ABCC2 ... distribution in obese patients [155] The majority of CP and IF elimination is by metabolic transformation in the liver; only 10-20% remain unchanged in the urine and only 4% in the bile [156] The initial...

Ngày tải lên: 14/09/2015, 14:06

... CD90 and CD105, weak staining for 3G5, and negative staining for low-affinity nerve growth factor receptor (LNGFR), STRO-1, CD34 and CD56 No staining was observed for the IgG control The staining ... temperature in streptavidin–peroxidase complex (1:500 in DPBS; Dako), rinsed in distilled water and incubated in fast-DAB (3,3'-diaminobenzidine) peroxidase substrate (Sigma) for minutes and counterstained ... The GAG content reflected proteoglycan biosynthesis and accumulation in the matrix, and under hypoxic conditions there was a 1.9-fold increase in the total GAG per aggregate In spite of the increased...

Ngày tải lên: 09/08/2014, 10:20

... staining predominantly the tunica adventitia consisting of supporting tissue in the vasculature, vWF (c, d) staining predominantly the tunica intima consisting of the endothelial layer and the ... CD90, and CD105, weak staining for 3G5, and negative staining for LNGFR, STRO1, CD34, CD56, and the IgG control The FGF-2-expanded cells are morphologically different from cells expanded in the ... doublings These cells retained cell surface staining similar to the original parent population, with consistent staining for the various markers identified above (data not shown) The staining...

Ngày tải lên: 09/08/2014, 10:23

... 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory anti-alpha5 and anti-beta1 monoclonal antibodies Evidence that the synergy sequence of fibronectin is ... PJ, Wu JE, Santoro SA 1995 ;The spatial and temporal expression of the alpha beta integrin and its ligands, collagen I, collagen IV, and laminin, suggest important roles in mouse mammary morphogenesis ... isolation and expansion Cytotherapy 75 Du F, Zhou Z, Mo ZY, et al 2006;Mixed macromolecular crowding accelerates the refolding of rabbit muscle creatine kinase: implications for protein folding in physiological...

Ngày tải lên: 11/09/2015, 09:16

... role in stabilizing the proteinprotein interface [31] All of the interactions reported in Fig in the range between and 6.0 A are identical in the major and minor fractions of the holoprotein in ... regulated The differences in stability between the two proteins (as indicated by the values of DGwater, Cm and Tg) hence arise from the changes in the surface of the protein because of the interactions ... protein The average major conformation was used for ligandprotein contact analysis (A) The interacting atoms are labeled The green dotted lines indicate hydrophobic interactions, and the blue lines...

Ngày tải lên: 16/03/2014, 10:20

... cap structure at the 50 end of the mRNA, involved in 30 UTR processing, splicing, and mRNA transport into the cytoplasm, has yet another role in translation by binding the cap-binding complex (Fig ... paracrine and endocrine factors and cell-cell interaction This interaction is initiated once PGCs enter the genital ridges during their homing period and is continued until fertilization PGCs and the ... mRNAs stored in the oocyte cytoplasm Proteins encoded by these mRNAs include the rapid inducer of G2/M progression in oocytes/Speedy (RINGO/Spy), cyclin B1, and cyclin-dependent protein kinase (Cdk2)...

Ngày tải lên: 22/03/2014, 20:21

... detected in patients with PTB in this study may therefore indicate the contribution of NK cells in the host immune response against MTB infection The immune response after MTB infection and disease ... the patients in this study Immunity against MTB comprises of a predominant cellular response and majority of researchers have dismissed the participation of B cells in defending against the infection ... response against MTB by increasing the infection inoculum (Vordermeier et al., 1996; Maglione et al., 2007) In addition the protection of mice against MTB infection by administration of intravenous...

Ngày tải lên: 29/03/2014, 03:20

... a peak increase of approximately 53% in the number of CD34 expressing cells and and a 90% increase in CD133 cells in circulation Furthermore, a significant augmentation of cells possessing hematopoietic ... capsules of Stem-Kine in the morning and two in the evening for 14 days Phenotypic assessment of circulating stem cells Peripheral blood mononuclear cells (PBMC) were isolated by the Ficoll-Hypaque ... medium containing 100 U/ml penicillin, 100 μg/ml streptomycin, and mM L-glutamine, and subsequently resuspended in 100 ul (0.5 M cells per 100 ul) of buffer (PBS+0.5% BSA) Cells were stained with...

Ngày tải lên: 18/06/2014, 16:20

... approximately 24, 48, and 72 hours The pH value was measured at the beginning and at the end of the test For the study with dimeglumine gadopentetate and gadofosveset trisodium, an incubating apparatus ... dimeglumine gadopentetate, gadoxetic acid disodium, and gadofosveset trisodium were investigated according to the guideline of the OECD 202 and the EC guideline part C.2.23,24 Different guidelines ... prepared for the control The algae were incubated in an incubator shaker under continuous light As a parameter for the growth of the algae population, the cell concentrations of the test and control...

Ngày tải lên: 18/06/2014, 16:20

... study of the endocrine cells within the pancreas, intestine, and stomach of the gar Gen Comp Endocrinol 1997, 106, 1-16 Guyot, E., Diaz, J P., Romestand, B and Connes, R Insulin during the early ... PP-immunoreactive cells Insulin is synthesized in the B cells of the pancreatic islets and regulates the serum glucose levels (Hsu and Crump, 1989) The regional distribution and relative frequency of the insulin-immunoreactive ... chromogranin containing cells in the porcine gastroenteropancreatic endocrine system Jpn J Vet Sci 1987, 50, 395-404 11 Jansson, L and Sandler, S L The influence of cyclosporin A on the vascular...

Ngày tải lên: 07/08/2014, 14:23

... lanoitubirtsid eht ,hguohtlA ]8,3[ sllec enircodneoruen lla fo ”srekram“ nommoc yltnacifingis a ,stneitap aitreni cinoloc nI slamina fo sutats evitsegid ot detaler ylraelc erew sllec RI-GC fo segnahc eht ... enircodne ni A ninargomorhc fo noitartsnomed lacimehcotsihonummI T aganawI ,N arahianaY ,F otaS ,H ikhO ,Y otomihsaH 01 892-782 ,25 ,9891 lotyC lotsiH hcrA saercnap enircodne eninac eht ni II ninargoterces ... yltnacifingis a ,noitidda nI ]5[ esaesid s’nhorC dna ]5[ sitiloc evitareclu ,]41[ romut ,]61[ noitapitsnoc cinorhc htiw stneitap fo mutcer eht ni desaercni osla yeht dna ]43[ asocum cinoloc ni...

Ngày tải lên: 07/08/2014, 18:21

... hematoxylin and eosin, and then with combined Luxol fast blue and cresyl violet to identify myelin and nerve cells [10] Percentages of myelinated areas in damaged spinal cords were calculated using the ... points post-SCI, at the start of treatment The scores for the ASC group increased to 1, 3.6 and 4.6 points at 3, and weeks, respectively (Fig 4) Scores in the C and V groups remained below point ... effective in canine SCI model, there was little histological evidence of spinal cord tissue regeneration [22] In this study, we examined whether canine ASCs could survive and integrate into neural cells...

Ngày tải lên: 07/08/2014, 23:22

... pouvoir and vouloir, such as their endings and their vowel sequence (eu in all the singular — je, tu, il/elle/on — and the third person plural — ils/elles; and ou in the nous and vous forms within the ... before the -ir For example, for the verb partir, get rid of the -tir for the singular and add -s, -s, -t Now for the plural, bring back the t from the infinitive and add -ons, -ez, -ent For the ... forms (je, tu, and il/elle/on) and add the following endings: -s, -s, nothing For the plural forms, drop the -dre and add the following ending: -ons, -ez, -nent The -prendre Verb Endings je -s nous...

Ngày tải lên: 08/08/2014, 10:22

... when the body is threatened by infection or injury In this setting T cells will become activated, and will recruit other T cells and other inflammatory cells and mediators to the site As the response ... fate of the TR cells induced at the site is not known — whether these cells exist only transiently then die, whether they persist in the body as TR cells, or whether they return to their previous ... involution, and antigen exposures, the development of CD4+CD25+ TR cells may occur in the periphery in man Induction of CD4+CD25+ TR cells in the periphery The induction of TR cells that resemble the...

Ngày tải lên: 09/08/2014, 01:24

... saline for 20 minutes and stored in 70% ethanol until processed for paraffin embedding Proteoglycan production in the ECM was visualised by toluidine blue staining (Sigma, St Louis, MO; 0.1% in ... and assayed for sulphated proteoglycan production using the DMB assay [26] Scoring of immunohistochemistry and toluidine blue staining Scoring of slides from immunohistochemical staining of cellsurface ... capable of differentiating into adipocytes, chondrocytes and osteoblasts, and, more recently, have been shown to differentiate into insulin-, somatostatinand glucagon-expressing cells [13] AD-MSC...

Ngày tải lên: 09/08/2014, 10:23

... analysed according to Scatchard, 1949 [35] The specific binding was determined by subtracting non-specific binding from the total The binding parameters, maximal binding (Bmax) and equilibrium ... receptors in the cerebellum and brain stem The binding data were confirmed by competition binding assay with [3H] GABA against different concentrations of GABA GABA affinity in the cerebellum and brain ... half the specific binding was defined as EC50, which is same as IC50 The affinity of the receptor for the competing drug is designated as Ki and is defined as the concentration of the competing...

Ngày tải lên: 10/08/2014, 05:21

... AK1C1, AK1C3, brain-type fatty acid-binding protein (FABP) and b-enolase On the other hand, those in SCLC were brain acid soluble protein (BASP), secretagogin (SEGN), fascin and neural cell adhesion ... and SCLC, 93 in the overlap only between LCNEC and LCC, and 453 in the overlap among three groups; SCLC contained a total of 1,096 including 362 unique, 100 in the overlap only between SCLC and ... will therefore be present in the region (x>3.84, z>3.84 corresponding to p < 0.05 each) on the x-z plane, those in SCLC in the region (y>3.84, z>3.84) on the y-z plane and those in LCC in the...

Ngày tải lên: 10/08/2014, 09:22