stem cells in the developing and adult nervous system

... lipid-binding protein, intestinal 15 kDa protein, ileal bile acid-binding protein and gastrotropin, reflecting the speculations of authors on its intracellular function(s) In vitro binding assays ... adrenal gland Sato et al [13] also detected rat FABP6 in the adrenal gland and ovary In adult mouse, Fabp6 transcripts were only detected by blot hybridization in the intestine, and not in the liver, ... found in the ovary and adrenal gland In fishes, the adrenal homolog is not as compact as the adrenal gland found in mammals In fishes, adrenal tissue exists as aminergic chromaffin and inter-regnal cells, ...

Ngày tải lên: 07/03/2014, 06:20

... the area of the embryo containing the pancreas were obtained and the pancreas outlined The brightness and contrast of some of the images were altered using Photoshop to better assess the staining ... the GenePaint database, we analyzed the in situ hybridization staining pattern in the pancreas from TS22 whole embryo sections In sum, 601 of the genes showed informative staining, and these were ... to group the genes Heatmaps of the relative expression levels of the genes in the SOTA groups, including the SOTA centroid, with peak expression in (a) the islets, (b) the TS26 developing pancreas,...

Ngày tải lên: 14/08/2014, 20:22

... miRNAs1expressed in proliferating and/ or differentiating cells in the developing and adult zebrafish brain miRNAs expressed in proliferating and/ or differentiating cells in the developing and adult zebrafish ... expressed in cells associated with the visual system, including retinal amacrine cells (INL) and ganglion cells (GCL in Figure 2h; Additional data files 13 and 14) This pattern is highly reminiscent ... in the adult brain as it is in the larval brain For instance, in both larva and adult, periventricular cells in the medial dorsal subpallium express miR-92b (compare Sd in Figure 1a with Vd in...

Ngày tải lên: 14/08/2014, 08:20

... developed a system of adenosine-releasing fibroblasts with inactivated adenosine kinase and adenosine deaminase These cells were transplanted into brain ventricles in a rat epilepsy model and were ... (Kim et al., 1996) The first DNA-binding domain used was a fruit fly homeobox domain, followed by zinc-finger DNA binding domain and yeast Gal4 DNA-binding 17 domain (Kim and Chandrasegaran, 1994; ... fusion proteins of a DNA-binding zinc finger domain and a double strand break-inducing nuclease domain Dimerization of nuclease domains is necessary for target cleavage The cellular machinery for...

Ngày tải lên: 26/11/2015, 09:54

... proinflammatory cytokine IL-6 Interestingly, the UC-MSCs could downregulate the IL-6 production in the transwell but not the cell-to-cell contact system (Figure 3c) both in single time point and in dynamic ... line with the induction of Tregs in vitro and in vivo in our study Moreover, in our experiments we did not find UC-MSCs in the joint of the CIA mice The short-term presence of UC-MSCs in the spleen ... in the transwell system or cocultured in the cell-to-cell contact system in 24-well plates After 72 hours, MMP9 in culture supernatants were determined MMP9 production was inhibited both in the...

Ngày tải lên: 12/08/2014, 15:22

... into an oocyte or fused with an ES cell, the underlying mechanisms remain obscure Using the ES fusion system, Smith has found an unexpected role for the homeodomain-containing protein Nanog in ... germ cells and ES cells is their epigenetic imprint status Shinohara reported that while the spermatagonial cells retain a stable androgenic imprint over long-term culture, the pluripotent cells ... by our inability to derive rat ES cells Shinohara hinted that he was making progress in applying his findings to the rat Finally, Tariq Enver (University of Oxford, UK) described a mathematical...

Ngày tải lên: 14/08/2014, 16:21

... temperature in streptavidin–peroxidase complex (1:500 in DPBS; Dako), rinsed in distilled water and incubated in fast-DAB (3,3'-diaminobenzidine) peroxidase substrate (Sigma) for minutes and counterstained ... The GAG content reflected proteoglycan biosynthesis and accumulation in the matrix, and under hypoxic conditions there was a 1.9-fold increase in the total GAG per aggregate In spite of the increased ... antibody (green), and 4',6-diamidino-2-phenylindole (blue) Results showed strong staining for CD13, CD29, CD44, CD90 and CD105, weak staining for 3G5, and negative staining for low-affinity nerve growth...

Ngày tải lên: 09/08/2014, 10:20

... staining predominantly the tunica adventitia consisting of supporting tissue in the vasculature, vWF (c, d) staining predominantly the tunica intima consisting of the endothelial layer and the ... CD90, and CD105, weak staining for 3G5, and negative staining for LNGFR, STRO1, CD34, CD56, and the IgG control The FGF-2-expanded cells are morphologically different from cells expanded in the ... doublings These cells retained cell surface staining similar to the original parent population, with consistent staining for the various markers identified above (data not shown) The staining...

Ngày tải lên: 09/08/2014, 10:23

... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... PJ, Wu JE, Santoro SA 1995 ;The spatial and temporal expression of the alpha beta integrin and its ligands, collagen I, collagen IV, and laminin, suggest important roles in mouse mammary morphogenesis ... and differentiation Arthritis Res Ther (1):204 121 Owen M, Peck W 1985;Lineage of osteogenic cells and their relationship to the stromal system In ed Bone and MineralAmsterdam Elsevier pp :1-25...

Ngày tải lên: 11/09/2015, 09:16

... only detected in the olfactory vesicles of embryos and larvae (Fig 5), and in the liver, intestine, muscle, brain, heart, eye, gills, ovary, testis, skin, kidney and swimbladder in adult zebrafish ... that the levels of fabp10b mRNA in muscle and heart were 6–24 times higher than in brain, eye, gills, testis, skin, kidney and swimbladder, and 320–650 times higher than in liver, intestine and ... Intracellular lipid-binding proteins and their genes Annu Rev Nutr 17, 277–303 Schaap FG, van der Vusse GJ & Glatz JFC (2002) Evolution of the family of intracellular lipid binding proteins in...

Ngày tải lên: 16/03/2014, 00:20

... cap structure at the 50 end of the mRNA, involved in 30 UTR processing, splicing, and mRNA transport into the cytoplasm, has yet another role in translation by binding the cap-binding complex (Fig ... ball, which introduces the clinical and the ethical views of the gamete generation from stem cells, probably one of the main key points of the stem cell field in reproductive medicine, is by two ... paracrine and endocrine factors and cell-cell interaction This interaction is initiated once PGCs enter the genital ridges during their homing period and is continued until fertilization PGCs and the...

Ngày tải lên: 22/03/2014, 20:21

... detected in patients with PTB in this study may therefore indicate the contribution of NK cells in the host immune response against MTB infection The immune response after MTB infection and disease ... the patients in this study Immunity against MTB comprises of a predominant cellular response and majority of researchers have dismissed the participation of B cells in defending against the infection ... response against MTB by increasing the infection inoculum (Vordermeier et al., 1996; Maglione et al., 2007) In addition the protection of mice against MTB infection by administration of intravenous...

Ngày tải lên: 29/03/2014, 03:20

... capsules of Stem- Kine in the morning and two in the evening for 14 days Phenotypic assessment of circulating stem cells Peripheral blood mononuclear cells (PBMC) were isolated by the Ficoll-Hypaque ... a peak increase of approximately 53% in the number of CD34 expressing cells and and a 90% increase in CD133 cells in circulation Furthermore, a significant augmentation of cells possessing hematopoietic ... where recruited into the study after understanding and signing informed consent Exclusion criteria included: systemic immune-compromised state, ongoing infection or disease conditions, and significant...

Ngày tải lên: 18/06/2014, 16:20

... represents the rotation of the foot (external/internal rotation) in respect to the walking direction and is defined as the angle formed with the line of progression and the segment connecting the marker ... in sitting, kneeling, standing and walking is delayed compared with children with the same age These patients develop their typical gait pattern already influenced by obesity In adult life, the ... during standing and walking, revealed a relevant increase in the foot surface in contact with the ground This would predispose to the development of a pathological foot, as demonstrated by the...

Ngày tải lên: 19/06/2014, 10:20

... cytoplasmic intermediate filament protein found in astrocytes They maintain the structural integrity of astrocytes, especially when these cells undergo hypertrophy and hyperplasia in response to a non-invasive ... to be increased following toxic insult to the CNS in many studies A single subcutaneous injection (50 μg/kg bw, 1/2 LD50) of the cholinesterase inhibitor Sarin was found to significantly increase ... alone and in combination, showed increased GFAP expression in offspring in the CA1 sub-field of the hippocampus, and white matter and granular cell layer of the cerebellum [17,18] In the present study,...

Ngày tải lên: 20/06/2014, 00:20

... VASA-positive cells (arrows) in the ovarian surface epithelium and cortex (A, B) Smaller cells with diameters of to μm in the epithelium (C-F) Bigger cells with diameters of 10 μm in the epithelium ... primitive oocyte-like cells were released from their “chambers” in the ovarian surface epithelium and started to change into hypertrophic/necrotic cells (Figures and 6) and further into autofluorescent ... oocyte-like cells in the adult human ovarian surface epithelium as related to epithelial ovarian cancer These cells resembled primitive oocytes in the ovarian surface epithelium of fetal ovaries, and...

Ngày tải lên: 20/06/2014, 08:20

... Journal of the International AIDS Society 2004, 6:56 http://www.jiasociety.org/content/6/3/56 sharing, and education that must attend expanding global HIV/AIDS care initiatives Authors and Disclosures ... grants for clinical research and/ or honoraria for educational and/ or advisory activities from Bristol-Myers Squibb, Boehringer Ingelheim, Gilead Sciences, GlaxoSmithKline, Idenix, Merck, and Roche ... significant financial interests or relationships to disclose Craig McClure has no significant financial interests or relationships to disclose Craig Sterritt has no significant financial interests...

Ngày tải lên: 20/06/2014, 08:20

... of the endocrine cells within the pancreas, intestine, and stomach of the gar Gen Comp Endocrinol 1997, 106, 1-16 Guyot, E., Diaz, J P., Romestand, B and Connes, R Insulin during the early postembryonic ... chromogranin containing cells in the porcine gastroenteropancreatic endocrine system Jpn J Vet Sci 1987, 50, 395-404 11 Jansson, L and Sandler, S L The influence of cyclosporin A on the vascular ... PP-immunoreactive cells Insulin is synthesized in the B cells of the pancreatic islets and regulates the serum glucose levels (Hsu and Crump, 1989) The regional distribution and relative frequency of the insulin-immunoreactive...

Ngày tải lên: 07/08/2014, 14:23

... lanoitubirtsid eht ,hguohtlA ]8,3[ sllec enircodneoruen lla fo ”srekram“ nommoc yltnacifingis a ,stneitap aitreni cinoloc nI slamina fo sutats evitsegid ot detaler ylraelc erew sllec RI-GC fo segnahc eht ... enircodne ni A ninargomorhc fo noitartsnomed lacimehcotsihonummI T aganawI ,N arahianaY ,F otaS ,H ikhO ,Y otomihsaH 01 892-782 ,25 ,9891 lotyC lotsiH hcrA saercnap enircodne eninac eht ni II ninargoterces ... yltnacifingis a ,noitidda nI ]5[ esaesid s’nhorC dna ]5[ sitiloc evitareclu ,]41[ romut ,]61[ noitapitsnoc cinorhc htiw stneitap fo mutcer eht ni desaercni osla yeht dna ]43[ asocum cinoloc ni...

Ngày tải lên: 07/08/2014, 18:21

... sitset eht ni sllec gidyeL laititsretni ni nees saw gniniatsonummi DLP esnetnI selubut suorefinimes eht ni sllec detaitnereffidnu emos ni gniniatsonummi DLP kaew dewohs yrtsimehcotsihonummI sllec ... desaercnI giF htiw dezilausiv saw ytivitcaeronummI ydobitna DLP-itna deifirup-ytiniffa eht htiw tcaer ot dewolla dna deziniffaraped erew )kciht mµ 5( seussit deddebme-niffarap fo snoitceS yrtsimehcotsihonummI ... -lynofluslyhtemlynehp Mm ,ninitorpa lm/gµ 01 ,nitpepuel lm/gµ 01 ,lCaN Mm 051 ,SDS %1.0 ,etalohcyxoed %1 ,001-X notirT %1 ,)2.7 Hp( SEPEH Mm 02[ reffub yassa noitatipicerponummi ni dezinegomoh erew selpmas...

Ngày tải lên: 07/08/2014, 20:23