pluripotent stem cells in bone marrow and cord blood

... formation and maintenance of the histo-architecture Plasmonogen activator inhibitor-2 (PAI-2) Involved in the regulation and inhibition of binding between urokinase-type plasminogen activator and its ... protein for phosphoserin motifs Regulated the interactions and subcellular localization of signalling molecules γ-Catenin Forms a complex with α- and β-Catenin to Cadherins, that are involved in ... marginal and inter-individually variable Conclusion In conclusion, the protein expression profile in AML blasts collected from bone marrow aspirates in comparison to blasts from peripheral blood...

Ngày tải lên: 18/06/2014, 15:20

... ilumits cinegotim cificepsnon ro ralullec yb decudni noitarefilorp etycohpmyl-T sserppus sllec lamorts worram enob namuH MA innaiG ,S itnasirG ,P iccuettaM ,DP inognoL ,M isenaliM ,M ingaM ,C ... latipsoH gnihcaeT lacideM yranireteV ytisrevinU lanoitaN luoeS ta god lergnom gk 85 a fo noitces nairaseaC eht gnirud deniatbo saw BCU :noitcelloc lleC sCSM-BCU eninac fo snoitaraperP dehsilbatse saw ... yliad semit eerht tsael ta demrofrep saw noisserpxe reddalb launam ,dedeen fi dna ,tinu erac evisnetni na ni derotinom erew sgod eht ,noitarepo eht retfA h 21 retfa devomer neht dna ,erutus epyt...

Ngày tải lên: 07/08/2014, 23:22

... methylcytosine cells, chromatin of pluripotent cells appears to have unique features, such as the increased mobility of many structural chromatin proteins, including histones and heterochromatin protein ... of epigenetic patterns in defining cell identity, understanding and utilizing epigenomic mapping will become a necessity in both basic and translational stem cell research In this review, we strive ... Methods for making induced pluripotent stem cells: reprogramming a la carte Nat Rev Genet 2011, 12:231-242 Takahashi K, Yamanaka S: Induction of pluripotent stem cells from mouse embryonic and adult...

Ngày tải lên: 11/08/2014, 12:21

... embryonic stem cell; hiPSC, human induced pluripotent stem cell; iPSC, induced pluripotent stem cell; lincRNA, long-intergenic non-coding RNA; lncRNA, long non-coding RNA; mESC, mouse embryonic stem ... other non-coding RNA, such as long intergenic non-coding RNA (lincRNA) Conversely, Oct4, Sox2 and Nanog, in concert with Polycomb group proteins (PcG), bind lineage-specific and non-coding RNA genes, ... decrease in both Wdr5 binding and H3K4me3 levels at Oct4-Wdr5 co-bound promoters This indicates that Oct4 may be responsible for directing Wdr5 to ESC genes and main­ taining H3K4me3 open chromatin...

Ngày tải lên: 11/08/2014, 12:21

... hydroxydaunorubicin (Adriamycin), Oncovin (vincristine) and prednisone - rituximab (CHOP-R) protocol was started and, after six courses and restaging by computed tomography and bone marrow and peripheral blood ... MCL and a MZL, with the latter involving the bone marrow and peripheral blood An alternative diagnosis might be an association of MCL involving the spleen and an atypical MCL involving both bone ... embedded in paraffin, and cut into to μm sections Morphological evaluations were performed on hematoxylin-eosin, Giemsa and GordonSweet for reticulin-stained sections Immunohistochemical stainings...

Ngày tải lên: 10/08/2014, 23:20

... other cells by interacting with actin filaments in migrating cells Integrins can also act as signal transducer in the “inside-out signaling” model The activation of integrins is caused by talin binding ... profiling and ADF/cofilin, are also act as regulators in mediating actin polymerization by controling the availability of activated actin monomers, debranching and depolymerizing proteins and capping ... protein response XBP1 X-box binding protein-1 xii Chapter Introduction 1 Introduction 1.1 Bone Marrow Stem Cell 1.1.1 Characteristics of Bone Marrow Stem Cells 1.1.1.1 Differentiation Capacity Bone...

Ngày tải lên: 02/10/2015, 17:15

... hematoxylin and eosin, and then with combined Luxol fast blue and cresyl violet to identify myelin and nerve cells [10] Percentages of myelinated areas in damaged spinal cords were calculated using ... stained with Alizarin red S D: Intense Alizarin red S stained after weeks incubation at osteogenic media and confirmed calcium deposition A and B: Oil red O stain, C and D: Alizarin red S stain, ... canine spinal cord injury model free from laminectomy Brain Res Brain Res Protoc 2005, 14, 171-180 11 Gimble J, Guilak F Adipose-derived adult stem cells: Transplantation of ASCs in spinal cord...

Ngày tải lên: 07/08/2014, 23:22

... resuspended in complete DMEM (containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin), and plated at to × 107 nucleated cells per 75 cm2 flask (Falcon, Beckton Dickinson Labware, Franklin ... cell/mL in serum-free DMEM containing mM pyruvate, 1% ITS + Premix (insulin, transferrin and selenous acid containing culture supplement), 37.5 mg/mL ascorbate2-phosphate and 10-7 M dexamethasone (all ... of the double labelling staining solution for 10 minutes at room temperature After staining, aggregates were washed five times with 100 μL of binding buffer, fixed overnight in PBS-buffered 4%...

Ngày tải lên: 09/08/2014, 14:22

... were fixed in formalin (4%) until further procession Formaline-fixed microspheres were dehydrated in increasing ethanol concentrations (50%, 75%, 90% and 100%) and embedded in paraffin (Paraplast ... cultivation in the presence of osteoinductive stimuli Numerous living cells were detected in the mineralized centre of the spheres by means of toluidine blue staining (Figure 1) Generally, the mineralization ... in bone tissue engineering: lessons from the design and synthesis of invertebrate skeletons Bone 2002, 30:810-815 16 Bielby RC, Boccaccini AR, Polak JM, Buttery LD: In vitro differentiation and...

Ngày tải lên: 11/08/2014, 20:21

... al., 1996) The first DNA-binding domain used was a fruit fly homeobox domain, followed by zinc-finger DNA binding domain and yeast Gal4 DNA-binding 17 domain (Kim and Chandrasegaran, 1994; Kim ... developed a system of adenosine-releasing fibroblasts with inactivated adenosine kinase and adenosine deaminase These cells were transplanted into brain ventricles in a rat epilepsy model and were ... fusion proteins of a DNA-binding zinc finger domain and a double strand break-inducing nuclease domain Dimerization of nuclease domains is necessary for target cleavage The cellular machinery for...

Ngày tải lên: 26/11/2015, 09:54

... a 45-minute incubation in peroxidase-labeled streptavidin (from DAKO kit) Finally the sections were stained with diaminobenzidine-H2O2 solution at room temperature The number of stained cells ... glycerol), containing 1.0 mM phenylmethylsulfonyl fluoride and μg/ml aprotinin Samples were then centrifuged at 12,000 rpm for 20 at °C and the supernatant containing total protein was denatured and separated ... shown to protect mice against hydrocoticoid-induced inhibition of IFN-γ, IL-2, IL-4 and IL-10 transcription [24] In addition, vitamin B12, folinic acid and biotin identified in AP may also contribute...

Ngày tải lên: 02/11/2012, 10:19

... embryonic stem cells into ovarian structures containing oocytes Stem Cells 2006; 24:266–273 24 Qing T, Shi Y, Qin H, et al Induction of oocyte-like cells from mouse embryonic stem cells by co-culture ... pivotal role in translation initiation by binding eIF4E and eIF4A, by forming a bridge between the mRNA and the ribosome, and by binding PABPC1 to facilitate translation of poly(A)-containing mRNAs ... stored mRNAs RINGO/Spy protein is absent in the oocyte cytoplasm, while its mRNA is maintained in a dormant state This is achieved by binding of Pumilio-2, an RNA-binding protein initially described...

Ngày tải lên: 22/03/2014, 20:21

... tissue injury [45-47] Hematopoietic stem cells are being increasingly recognized as having diverse non-hematopoietic functions including production of angiogenic cytokines [48], and acting as an “innate” ... required to ingest two capsules of Stem- Kine in the morning and two in the evening for 14 days Phenotypic assessment of circulating stem cells Peripheral blood mononuclear cells (PBMC) were isolated ... a peak increase of approximately 53% in the number of CD34 expressing cells and and a 90% increase in CD133 cells in circulation Furthermore, a significant augmentation of cells possessing hematopoietic...

Ngày tải lên: 18/06/2014, 16:20

... lymphopoiesis, bone marrow myelopoiesis and organogenesis [8-11] Both SDF-1-deficient and CXCR4-deficient mice die perinatally and have very few hematopoietic stem cells and progenitors within their bone marrow ... increased numbers of OCPs in both bone marrow and blood, whereas SDF-1 or GCSF administration triggers a rapid release of cells from the bone marrow – and the total bone marrow cell number is consequently ... chronic inflammatory arthritis have an increased frequency of OCPs in peripheral blood and spleens, and that TNF promotes the release of bone marrow OCPs into the bloodstream [3-5] To investigate...

Ngày tải lên: 09/08/2014, 10:23

... Long-term changes in the immunoglobulin expression of B cells following rituximab treatment Isolated bone marrow mononuclear cells were stained immunoglobulin expression of B cells following rituximab ... B cells in BM The remaining BM B cell population consists of CD27IgD- immature B cells, and mostly CD27+IgD- switched memory B cells Simultaneously, the levels of RF and Ig-secreting cells in ... in the immunoglobulin expression of B cells following rituximab treatment Isolated bone marrow mononuclear cells were stained the immunoglobulin expression of B cells following rituximab treatment...

Ngày tải lên: 09/08/2014, 14:22

... such as intestine [52] and from the ectodermal tissues including skin [53], deciduous teeth [54] and nerve tissue [8,9,55,56] ASCs originate during ontogenesis and remain in a marginal area in a ... results in a cell-mediated autoimmune attack against insulin-secreting pancreatic b -cells Insulin regulates glucose homeostasis and, in particular, it reduces glycemia when glucose exceeds in blood ... islets and reintroduce physiological secretion of human insulin AHSCT improves b -cells function and frequently decreases the exogenous insulin need [20] or induces a persistent insulin independence...

Ngày tải lên: 10/08/2014, 10:20

... observed in more recent GWASs [23] Linking GWAS findings to AD pathogenesis Both APOE and CLU are lipoproteins that are found in the brain, with APOE being the predominant brain lipo­ protein (reviewed ... lines and patients By comparing iPSC-derived neurons and/ or glia between individuals, it may be possible to validate findings from GWAS and animal models studies and to identify novel initiating ... disease and to investigate disease phenotypes in vitro [31,32] iPSCs are most commonly made by taking a small skin biopsy from a patient, expanding the biopsy into primary fibroblasts, and transducing...

Ngày tải lên: 11/08/2014, 12:21

... were decalcified and paraffinembedded using standard histologic techniques Serial μm sections were cut and stained with hematoxylin and eosin to examine morphologic features and assess the histologic ... UC-MSCs, indicating that UC-MSCs might be potentially important in the inhibition of bone erosion in RA T cells are believed to play a critical role in orchestrating the inflammatory response in RA ... reduced inflammatory responses in CIA There were reduced levels of proinflammatory cytokines and chemokines (TNF-a, IL-6 and MCP-1) and increased levels of the anti-inflammatory/regulatory cytokine...

Ngày tải lên: 12/08/2014, 15:22

... significant role in RA inflammation [2,8,9] The BM contains three types of stem cells: hematopoietic stem cells (HSCs), which produce all the mature blood lineages for leukocytes, erythrocytes, and platelets; ... was intraoperatively obtained from the nine RA patients and the 10 OA patients while undergoing joint arthroplasty Detailed patient characteristics, medication usage, including disease-modifying ... including disease-modifying agents and steroids, and laboratory testing, including rheumatoid factor (RF) and C-reactive protein (CRP), of the nine RA patients are shown in Table None of the 10 OA patients...

Ngày tải lên: 12/08/2014, 17:21

... Problems in Medicine and Biomedical and Behavioral Research: Defining death: a report on the medical, legal and ethical issues in the determination of death USGPO Washington DC; 1981 Landry DW, ... Ratio of inner cell mass and trophoblastic cells in blastocysts derived from porcine 4- and 8cell embryos and isolated blastomeres cultured in vitro in the presence or absence of protein and human ... end of the beginning for pluripotent stem cells Nature 2001, 414:92-97 23 24 25 26 Doerflinger RM: The ethics of funding embryonic stem cell research: a Catholic viewpoint Kennedy Inst Ethics J...

Ngày tải lên: 13/08/2014, 23:20