4 cell culture condition and cb hsc expansion

... Cell Culture Table Definition of Cell Culture Terms Term Definition Cell culture Maintenance of dissociated cells in culture Tissue culture Maintenance of tissue explants in culture Cell line A culture ... Barnes, D and Sato, G (1980) Methods for growth of cultured cells in serum-free medium Anal Biochem 102, 255–270 21 Ham, R G and McKeehan, W L (1978) Development of improved media and culture conditions ... adherent cultures) Phosphate-buffered saline (PBS) for adherent cultures Tissue culture plastics 2.3 Subculture Any adherent cell culture, for example, SKOV-3 ovarian cancer cell line Tissue culture...

Ngày tải lên: 10/04/2014, 11:11

... Cooper and Moses Cooper At various times the child would stand against the board and their height would be measured by drawing a line and dating the line At the left is the full board and at ... height of my grandchildren at random occasions, usually family gatherings When measuring time occurs the grandchildren stand against the board, a line is drawn at the top of their head, and the date ... rate changes before and after this point which vary with the exact points at which the growth period starts and stops These are not regular in their appearance and pattern, and occur because of...

Ngày tải lên: 13/08/2014, 23:20

... synthesized EUK-8 and EUK-134 and helped to write the manuscript providing useful suggestions GCB conceived of the study, and participated in its design and coordination, and wrote Introduction and Discussion ... Materials and methods Alzheimer's disease is characterised by neuritic plaques that contain dead and dying neurons and their processes, inflammatory-activated microglia and β-amyloid peptides Aβ1–40 and ... smaller fractions and stored at -20°C After rethawing the fibrillar state of the peptide remained unchanged Preparation of pure microglial culture Primary mixed astrocyte and microglial cultures were...

Ngày tải lên: 19/06/2014, 22:20

... http://www.aidsrestherapy.com/content/6/1/27 ml) and AMD 3100 (100 ng/ml) to block CCR5 and CXCR4 Culture supernatants were harvested at days and and HIV replication was determined by measuring ... 37°C with 5% CO2 for hours, washed thoroughly and cultured in MEM media with10% FBS and antibiotics Appropriate controls included uninfected cells, and cells treated with co-receptor antagonists ... amplified by nested PCR and sequenced using primers ED31 5'CCTCAGCCATTACACAGGCCTGTCCAAAG and ES8 5'CACTTCTCCAATTGTCCCTCA and sequences were edited, aligned in Clustal program and translated into...

Ngày tải lên: 10/08/2014, 05:21

... Musculoskeletal disorders and orthopedic conditions Jama 2001, 285:619-623 Meyer U, Wiesmann HP: Bone and cartilage tissue engineering Heidelberg, Berlin, Tokyo, New York: Springer; 2005 Handschel J, Berr ... 22:138-147 30 Rando TA: Stem cells, ageing and the quest for immortality Nature 2006, 441:1080-1086 31 Gerber I, ap Gwynn I: Influence of cell isolation, cell culture density, and cell nutrition ... cell mass of blastocysts extracted from C57BL/6 mice and tested positive for the stem cell markers Pouf1 (alias Oct4) and Foxd3 [18] Cells were cultured in Dulbecco’s modified Eagle medium (DMEM,...

Ngày tải lên: 11/08/2014, 20:21

... contributions HZ and KL conceived the study and designed the experiments HSS, JL, SS and LY carried out the experimental work HSS, HZ and KL wrote the paper All Authors have read and approved the ... sec, for HCVcc in culture medium (A) and human serum (B), respectively, after the data were fit to a linear regression model (R2 = 0.9738 and 0.9891, respectively) HCVcc stocks, and that glutaraldehyde ... drying and environmental exposure to RT for at least 16 h [4] The results of the current study have demonstrated that JFH-1 virus (genotype 2a) grown in cell culture can survive 37°C and RT for and...

Ngày tải lên: 12/08/2014, 04:21

... confluence On the second day, culture medium containing 1% serum was added and the cells were cultured for 24 hours Culture medium with 1% serum was changed again and cells were cultured for another 24 ... contributions YS, HEG and ENH conceived the study and participated in its design and coordination YS wrote the manuscript, and analyzed the microarrays DRM and JSK provided surgical tissues and participated ... examined and compared the expression of ENPP1 and ANKH in OA meniscal cells and in normal meniscal cells Both microarray and real-time RT-PCR analyses indicated that the expression of ENPP1 and ANKH...

Ngày tải lên: 12/08/2014, 12:20

... (37°C) culture medium and centrifuged to remove the DMSO It was resuspended in culture medium and then transferred into cell culture flasks and cultured (passage for the cells after cryostorage) and ... to 95% confluency, cells were passaged 1:2 and a part of the cells was harvested Total RNA was extracted and stored at -70°C Culture conditions were (and have to be) kept constant during the experiments ... inactivated FCS (Gibco Life Technologies, Grand Island, NY, USA), 100 U/ml penicillin and streptomycin (PAA Laboratories GmbH, Linz, Austria) and cultured for four passages at 37°C in 10% carbon...

Ngày tải lên: 12/08/2014, 12:20

... vector and the insert were ligated in the ratio 1:3 overnight at 16 oC, and transformed into E coli (DH5 ) cells clones were picked and inoculated into overnight cultures Plasmids isolation and ... follow: Component 10x NEB buffer (New England Biolab) 0.1% BSA (New England Biolab) 10,000 U/ml Bgl II (New England Biolab) 20,000 U/ml HindIII (New England Biolab) Water pSuper (1µg) (600 ng/µl) ... 4 Materials and Methods 4.1 Cell culture 4.1.1 Routine cell line maintenance All cell cultures were maintained at 37 °C with 5% CO2 and were fed daily Feeder-free E14 Mouse...

Ngày tải lên: 11/09/2015, 16:02

... quantity and quality is not guaranteed to develop and expand the species distribution (Nghia N H., 1999) On the other hand, it is due to its poor regeneration and requirement of severe conditions ... Wickremesinhe and Arteca, 1994), MS (Murshige and Skoog, 1962) and Woody Plant Medium (WPM) (Loyd and McCown, 1981) have been used for the initiation, proliferation and maintenance of the callus and cell ... paclitaxel via calli and cell suspension cultures using different explants and media (Christen et al., 1991; Fett-Neto et al., 1992; Wickremeshinhe and Arteca, 1993) Suspension cell culture was also...

Ngày tải lên: 23/10/2015, 15:38

... 米粒大小对蛹虫草固体发酵子实体干重和培养基干重的影响 T abl e The ef f ect of rice grain siz e on d ry weight of f rui t body and m edium in C mi li tar is 米粒大小 结果与分析 米粒大小对蛹虫草固体发酵子实体干重和培 养基干重的影响 从表 可以看出 , 子实体干重最大和最小的两 组相差 ... 米粒大小对蛹虫草固体发酵子实体和培养基中虫草菌素含量 的影响 T abl e The ef fect of rice grain size on cordy cepin content in f ruit body and medium in C mi li tar is 米粒大小 Rice grain size 整米 子实体中虫草 培养基中虫草 总的虫草菌素 菌素含量/ % 菌素含量/ % 产量/(mg/瓶) ... 不同 pH 对蛹虫草固体发酵子实体干重和培养基干重的影响 Table 　T he ef f ect of medi um pH on dry w ei ght of frui t body and medi um i n C mi l it ari s pH 值 pH 5 6 7 子实体干重/(g/ 瓶) Weig ht o f f ruit body 47 81 61 59 52...

Ngày tải lên: 08/01/2017, 20:59

... command and a 1- or 2-pixel radius to snatch up those stray, deselected pixels The Smooth command can also eliminate jagged edges along the outside of your selection ߜ Expand: The Expand command ... modification commands The next group of commands in the Select menu actually holds six separate commands, including Refine Edge (discussed earlier in this chapter), and the five Modify commands, each ... the Grow and Similar commands, which are somewhat like the Magic Wand with Contiguous (Grow) and without Contiguous (Similar) selected on the Options bar (In fact, they use the Magic Wand’s Tolerance...

Ngày tải lên: 27/08/2012, 14:35

... Listen and read I/ Getting started II/ Listen and read New words D examiner aspect A B examination B examination C attend C attend D examiner A aspect Lesson : Getting started – Listen and read ... Lesson : Getting started – Listen and read I/ Getting started II/ Listen and read New words examiner (n) : Ng­êi chÊm thi examination (n) : Kú thi attend ... I/ Getting started II/ Listen and read New words examiner (n) : Ng­êi chÊm thi Kú thi examination (n) : tham gia attend (v) : khÝa c¹nh, mÆt aspect (n) : Direct and reported questions Grammar...

Ngày tải lên: 27/06/2013, 11:45

... Trong trường hợp có liên từ and giới từ of, ta phải xác đònh đầy đủ chủ từ túc từ chuyển đổi Ex:- Active: He and I see the film  Passive: The film is seen by him and me - Active: He bought a ... …………………………….………………………….…………………………… The winner of the marathon, who was breathing deeply and smiling at the crowd, raised her right hand and waved ………………………………………………….…………………………………………………………… ………………………… 10 Any ... ………….…………………………… Disney World is a famous amusement park It is located in Orlando, Florida, USA, and covers a large area of land ……………………………………………………………………………………………………………….……………………………… 10 Do you know...

Ngày tải lên: 17/08/2013, 19:10

... grandmother’s mom > việc xảy khứ hie What should I ay không còncall her? These are Nga and her grandmother Her grandmother is telling her about her life in the past Guessing what Nga’s grandmother ... to stay (2)…………… and help her after mom She used to look (3)…… …… ….her younger had brothers and sisters And her great-grandma (4)… wash to cook the meals, clean the house and (5)………the electricity ... ….her younger brothers and sisters And her great-grandma (4)… to cook the meals, clean the house and (5)………the clothes There wasn’t any (6)………… So her great -grandma had to everything without the...

Ngày tải lên: 27/09/2013, 01:10

... to cook meals , clean the house and wash the clothes d Her great-grandma used to lit the lamp and her great- grandfather used to tell stories e She asked her grandma to tell her the tale “ The ... trả lời a She used to live on a farm b Because she had to stay at home and help her Mom to look after her younger brothers and Nghe tick Hs đọc HS trả lời HS luyện tập HS thực hành hội thoại HS ... nghe đánh dấu tích việc mà bà Nga phải làm bảng phụ - Stay at home - look after younger brothers and sisters - empty the fish tank - Help mom - Watch TV - Feed the chicken/ buffalos Nội dung đoạn...

Ngày tải lên: 28/09/2013, 15:10

... Lesson LEARNING A FOREIGN LANGUAGE Lesson Getting started + Listen and read - an examiner = giám khảo - (to) attend = tham dự - a candidate = thí sinh -an oral examination = kỳ thi vấn đáp -an aspect ... First she asked me what my name was, and where I came from Paola : They were easy for you, weren’t they ? Lan : … Then she asked me why I was learning English, and if I spoke any other languages ... started-Listen and Read She asked me what my name was She asked me if/whether I spoke any others languages UNIT4: LEARNING A FOREIGN LANGUAGE Lesson 1: Getting started-Listen and Read The reported...

Ngày tải lên: 11/10/2013, 06:11

... Cdc28 and classes of vertebrate cyclins (D- and E-type cyclins in G1 and A -and B-type cyclins in G2/M) that control a family of Cdks (cyclindependent kinases) greatly enhanced our understanding ... emerged from distinct studies in yeast, flies, clams, and frogs, and eventually the conclusions drawn were tested and found to be operative also in mice and humans The power of the cell cycle theory ... 1997; Zachariae and Nasmyth 1999) These studies and others demonstrated the universality of the cell Modeling Cell Cycle Control and Cancer with pRB Tumor Suppressor 231 cycle, and strongly suggested...

Ngày tải lên: 25/10/2013, 21:20

... understand the relevance of pRb loss in development of cancer (reviewed in Weinberg 1995; Hanahan and Weinberg 2000; Harbour and Dean 2000) The molecular cloning of two other Rb-like genes, p107 and ... and/ or CIP/KIP family of cyclin dependent kinase inhibitors (CKI), resulting in the down-regulation of cyclin/Cdk activity and inhibition of pRb phosphorylation (Ruas and Peters 1998; Sherr and ... pancreatic and bladder carcinomas, amplification of cyclin D in breast, oesophagus and head and neck cancer, Cdk4 amplification or mutational activation in melanoma (reviewed in: Sherr 1996; Malumbres and...

Ngày tải lên: 25/10/2013, 21:20