422 minimized equations derived from table421
Tài liệu Báo cáo khoa học: Different modes of dipeptidyl peptidase IV (CD26) inhibition by oligopeptides derived from the N-terminus of HIV-1 Tat indicate at least two inhibitor binding sites doc
Ngày tải lên :
20/02/2014, 11:20
... prolinespecific proteinases: (I) Substrate specificity of dipeptidyl peptidase IV from pig kidney and proline-specific endopeptidase from Flavobacterium meningosepticum Biochim Biophys Acta 954, 161–169 Schon, ... Salahuddin, S.Z., Gallo, R.C & WongStaal, F (1990) Tat protein of HIV-1 stimulates growth of cells derived from Kaposi’s sarcoma lesions of AIDS patients Nature 345, 84–86 19 Viscidi, R.P., Mayur, K., ... the system to 700 K within 2000 fs and cooling to 100 K in 2000 fs the ligands not move far away from the ˚ enzyme at the high temperature, only about 10 A on average During the annealing phase...
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Tài liệu Báo cáo khoa học: Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix docx
Ngày tải lên :
20/02/2014, 23:20
... of genomic DNA purified from HeLa and WI-38 cells was performed using a probe from an exon of the Mcm2 gene that codes for amino acids 214–287 Expected bands were detected from HeLa and WI-38 DNA, ... medium) supplemented with 10% calf serum WI-38 cells obtained from RIKEN GenBank and SV40-transformed human fibroblasts (GM00637) purchased from Coriell Cell Repositories were cultured in DMEM supplemented ... human glyceraldehyde-3-phosphate dehydrogenase cDNA were purchased from Toyobo Northern blot analysis Total RNA was extracted from HeLa and WI-38 cells with phenol, and electrophoresed on agarose...
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Báo cáo khoa học: Study of synthetic peptides derived from the PKI55 ppt
Ngày tải lên :
07/03/2014, 05:20
... isoforms were obtained from Calbiochem (Milan, Italy), poly(vinylidene difluoride) membranes were from Bio-Rad Laboratories S.r.l (Milan, Italy) and PKC a, b1, b2 and f antibodies were from Santa Cruz ... peptides The level of PKC a, b1, b2 and f isoforms was also studied Results Synthesis of peptides derived from PKI55 and their inhibitory effect on rat brain PKC A series of peptides was synthesized ... isoforms and to assess their potential anti-inflammatory action Inhibitory effect of peptides derived from PKI55 on PKC isoforms Results obtained in a previous study of the inhibition properties...
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Báo cáo khoa học: "Data-Defined Kernels for Parse Reranking Derived from Probabilistic Models" docx
Ngày tải lên :
08/03/2014, 04:22
... reranking the top 20 parses from the probabilistic model This method achieves a significant improvement over the accuracy of the probabilistic model alone Kernels Derived from Probabilistic Models ... sum over parts of the tree In particular, this is impossible with the TOP and Fisher kernels derived from the SSN model Also, it isn’t clear whether the algorithm remains tractable for a large ... trained, so that they reflect what features of trees are relevant to parsing The kernel is then derived from this trained model in such a way as to maximize its usefulness for reranking We performed...
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Báo cáo Y học: IgE reactivity of tandem repeats derived from cockroach allergen, Bla g 1 docx
Ngày tải lên :
08/03/2014, 23:20
... lead the protein to the periplasmic fraction from which it would be easy to purify Recombinant Bla g expressed in Pichia Affinity-purified rBla g1 from methanol-induced Pichia contained four proteins ... lower levels (from 10 to 200 times lower, depending on the volume of the culture and incubation time) than the Pichia-inducible system Attempts to scale-up constitutive expression from Fig PCR ... purification through a 10A6 mAb column Eluted fractions 7–30 from a culture (lane 1); early (9–14, lane 2); and late (15–30 lane 3), fractions from another culture grown under the same conditions (B)...
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PRINCIPLES FOR THE RISK ANALYSIS OF FOODS DERIVED FROM MODERN BIOTECHNOLOGY
Ngày tải lên :
13/03/2014, 21:56
... Assessment of Foods Derived from Recombinant-DNA Animals (CAC/GL 68-2008) See footnote Reference is made to the Compilation of Codex Texts Relevant to Labelling of Foods Derived from Modern Biotechnology ... its nature and severity The safety assessment should include a comparison between the food derived from modern biotechnology and its conventional counterpart focusing on determination of similarities ... withstand scientific peer review 13 Risk assessment should apply to all relevant aspects of foods derived from modern biotechnology The risk assessment approach for these foods is based on a consideration...
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Báo cáo khoa học: Small peptides derived from the Lys active fragment of the mung bean trypsin inhibitor are fully active against trypsin pptx
Ngày tải lên :
15/03/2014, 09:20
... fragment could be separated from each other by reduction, and the long peptide chain still exhibited inhibitory activity after reoxidation [25] A 22-residue peptide derived from the long chain, with ... from Amersham Biosciences (Uppsala, Sweden) The ZORBAX 300 SB-C18 semipreparative column was from Agilent Technologies (Santa Clara, CA, USA) Trifluoroacetic acid (TFA) and acetonitrile were from ... obtained from Applied Biosystems (Foster City, CA, USA) Fmoc-Cys [trityl (Trt)] hydroxymethylphenoxymethyl polystyrene resin was obtained from PE (Rockford, IL, USA) Bovine trypsin was from Sigma...
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Relative risk of surface water pollution by E. coli derived from faeces of grazing animals compared to slurry application pptx
Ngày tải lên :
15/03/2014, 23:20
... drain¯ow from 29 May to 17 July and 180 mm from 17 July to 10 September In many summers virtually no drain¯ow occurs at this site in the period from May to September Drainage from the grazed plots from ... ratio of the losses from grazing to those from slurry during the period from t = to t = T can now be compared: losses from grazing Cs
0 À Cg
T Cs
0 À Cs
T losses from slurry h ÀkT ... from grazing animals DISCUSSION The foremost factor in¯uencing the potential pollution of surface waters by E coli from animal faeces is the relative farm scale inputs from fresh faeces and from...
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Báo cáo khoa học: Oxygen control of nif gene expression in Klebsiella pneumoniae depends on NifL reduction at the cytoplasmic membrane by electrons derived from the reduced quinone pool doc
Ngày tải lên :
17/03/2014, 10:20
... Southern blot analysis was performed using a ndh probe derived from K pneumoniae ssp pneumoniae and SmaI digested chromosomal DNA derived from K pneumoniae M5a1, K oxytoca and K pneumoniae ssp ... inside-out vesicles derived from K pneumoniae cells grown under anaerobic conditions and thus containing the anaerobic respiratory chain (Figs and 2) Three other lines of evidence derived from in vivo ... chromosomal nifK¢¢lacZ fusion (A) Synthesis rates of b-galactosidase from the nifHDK promoter were determined from the slope of these plots from at least five independent experiments and are presented...
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NOT MEASUREMENT SENSITIVEMIL-HDBK-17-4 Volume 4 of 4 21 September 1999DEPARTMENT OF DEFENSE pdf
Ngày tải lên :
22/03/2014, 13:20
... the secant drawn from the origin to any specified point on the stressstrain curve 45 MIL-HDBK-17-4 Modulus, Tangent The ratio of change in stress to change in strain derived from the tangent ... value is computed from the flexure equation: Fb = where Mc I 1.1.7(a) M = maximum bending moment computed from the maximum load and the original moment arm, c = initial distance from the neutral ... is on data obtained from ASTM standard test methods for advanced composites, but where an ASTM test method has been deemed inappropriate or is not yet available, or when data from a nonstandard...
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How Do Earnings Change When Reservists Are Activated - A Reconciliation of Estimates Derived from Survey and Administrative Data docx
Ngày tải lên :
23/03/2014, 02:20
... reservists change when they are activated as derived from SOFS-R data with analogous estimates derived from administrative data.3 To so, we match survey responses from the 2004 and 2005 SOFS-R to the ... bias estimates derived from the SOFS-R data On the other hand, civilian earnings may not be recorded perfectly in our administrative data sources, leading to biased estimates derived from those data ... Change Derived from SOFS-R and Administrative Data Estimates of Average Monthly Earnings Change Derived from SOFS-R...
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Báo cáo khoa học: A peptide derived from cyclin-dependent kinase activator (p35) specifically inhibits Cdk5 activity and phosphorylation of tau protein in transfected cells pdf
Ngày tải lên :
23/03/2014, 21:21
... Reagent were purchased from Invitrogen (Carlsbad, CA) pGEM-T vector was obtained from Promega Corporation (Madison, WI, USA) The Cdk5 inhibitor, roscovitine, was obtained from Biomol Research Laboratories, ... (5¢TGCCTGGGTGAGTTTCTC-3¢) and a reverse primer (5¢-TGGGTCGGCATTTATCTG-3¢) derived from p35 (Fig 1A) A CMV-tau fragment (amino acids 181–242) was generated by PCR from a rat brain cDNA library The primer was designed ... sequencing Cell culture and transfection Fig Identification of the Cdk5/p25 inhibitory peptide (CIP) derived from p35 (A) Mapping of p25, p16 and CIP to p35 (human sequence) Red segments are the alpha...
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Báo cáo khoa học: Novel strategy for protein production using a peptide tag derived from Bacillus thuringiensis Cry4Aa pptx
Ngày tải lên :
29/03/2014, 09:20
... morphology of the GST–4AaCter inclusions looked different from protein crystal as imagined in general Enzyme activity of GST–4AaCters derived from inclusions Upon incubation of the inclusions in alkali ... Nine peptide sequences derived from the Cry4Aa C-terminal region were fused with GST and expressed in E coli strain BL21 Briefly, the DNA fragment encoding the polypeptide from I696 to P851 of Cry4Aa ... GST activity ranging from 65 to 110 lmolÆmin)1Æ nmol)1 (Fig 4) Thus, because relatively high GST activity was observed, the inclusion formed by 4AaCters seemed to be different from the inclusion...
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Báo cáo khoa học: Metabolic fate of L-lactaldehyde derived from an alternative L-rhamnose pathway ppt
Ngày tải lên :
30/03/2014, 02:20
... fate of l-lactaldehyde derived from the alternative l-rhamnose pathway in P stipitis is dehydrogenation by LADH Purification of LADH from P stipitis (PsLADH) PsLADH was purified from P stipitis cells ... dehydrogenase for l-lactaldehyde and d-lactaldehyde seems to derive from the same enzyme, and that NADP+-dependent activity may be derived from the concomitant activity of other constitutively expressed ... l-lactaldehyde, the reaction product of the enzymes from E coli [4,5], Methanocaldococcus jannaschii [10] and S cerevisiae [9] is l-lactate (EC 1.2.1.22), whereas that from rat liver is methylglyoxal (EC 1.1.1.78)...
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Báo cáo khoa học: An asymmetric ion channel derived from gramicidin A Synthesis, function and NMR structure ppt
Ngày tải lên :
30/03/2014, 15:20
... this way, the structure starts from an N-terminus and ends at a C-terminus, which is FEBS Journal 272 (2005) 975–986 ª 2005 FEBS Asymmetric ion channel derived from gramicidin A similar to a ... 1H-NMR (500 MHz, FEBS Journal 272 (2005) 975–986 ª 2005 FEBS X Xie et al Asymmetric ion channel derived from gramicidin A OH O Ala-D-Val-Val-D-Val-Trp-D-Leu-Trp-D-Leu-Trp-D-Leu-Trp O H N OTBDPS Val-Gly ... spectrum was seen in the presence of eq CsCl Just one positive maximum 977 Asymmetric ion channel derived from gramicidin A B 120 100 80 60 40 20 -20 -40 -60 -80 -100 Mean Res Ellipt.*103 Mean Res Ellipt.*103...
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Báo cáo khoa học: Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin pptx
Ngày tải lên :
31/03/2014, 09:20
... BmKIT2 are derived from Buthus martensii Karsch; BaIT2 from Buthacus arenicola; LqqIT2 from Leiurus quinquestriatus quinquestriatus; LqhIT2 from Leiurus quinquestriatus hebraeus; BjIT2 from Buthotus ... [37], CssII [38]; AaHIT2 is anti-insect toxin purified from the venom of the Scorpion Androctonus australis Hector; CssII, b-type antimammal toxin from Centruroides suffuses suffuses) They were characterized ... patch-clamp recording Control of sodium currents recorded by stepping up the membrane from )80 to +30 mv in 10 mV increments from the holding potential of )80 mv on ventricular myocyte and DRG neuron A...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
Ngày tải lên :
18/06/2014, 15:20
... muscle actin; hESECs+MCs: a mixture of ECs and MCs derived from human ES cells; hES-ECs: ECs derived from human ES cells; hESMCs: MCs derived from human ES cells http://www.translational-medicine.com/content/6/1/54 ... Characterization of the transplanted vascular cells derived from human ES cells (HES-3) Characterization of the transplanted vascular cells derived from human ES cells (HES-3) A, Flow cytometric analysis ... expanding not only ECs but also MCs derived from these ECs in the early differentiation stage in vitro In the present study, we examined whether ECs and MCs derived from human ES cells could serve...
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