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Báo cáo Y học: Modelling of simple and complex calcium oscillations From single-cell responses to intercellular signalling pdf

Báo cáo Y học: Modelling of simple and complex calcium oscillations From single-cell responses to intercellular signalling pdf

... (19 99) Regulation of total mitochondrial Ca2+ in perfused liver is independent of the Modelling calcium oscillations (Eur J Biochem 269) 13 5 3 16 8 16 9 17 0 17 1 17 2 1 73 17 4 17 5 17 6 17 7 17 8 17 9 18 0 ... Cacyt, Cam, Caer Cacyt, B, ER transmembrane potential [12 ]a [12 6 ,18 6 ,18 9] [18 6] [12 ]b [ 13 0 ] [16 ] a [72] [99 ,12 5]b [89,92 ,18 6] [ 71, 115 ,16 2]c [11 1] Using the conservation relation Cacyt + Caer/qer ... 18 0 18 1 18 2 1 83 18 4 18 5 18 6 18 7 18 8 18 9 19 0 permeability transition pore Am J Physiol 274 (Cell Physiol 45), C1297–C 13 0 2 Rinzel, J (19 78) On repetitive activity in nerve Fed Proc 37 , 27 93 2802...

Ngày tải lên: 24/03/2014, 00:21

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báo cáo khoa học: " Simultaneous measurement of sensor-protein dynamics and motility of a single cell by on-chip microcultivation system" pps

báo cáo khoa học: " Simultaneous measurement of sensor-protein dynamics and motility of a single cell by on-chip microcultivation system" pps

... isolated cells Biochem Biophys Res Commun 20 03, 30 5: 534 -540 Mesibov R, Adler J: Chemotaxis toward amino acids in Escherichia coli J Bacteriol 19 72, 11 2 : 31 5 -32 6 Publish with Bio Med Central and every ... 19 93, 259 :17 17 -17 23 Lybarger SR, Maddock JR: Clustering of the chemoreceptor complex in Escherichia coli is independent of the methyltransferase CheR and the methylesterase CheB J Bacteriol 19 99, ... 20 01, 1: 50-55 Wakamoto Y, Inoue I, Moriguchi H, K Yasuda: Analysis of singlecell differences using on-chip microculture system and optical trapping Fresenius' J Anal Chem 20 01, 37 1: 276-2 81 Umehara...

Ngày tải lên: 11/08/2014, 00:22

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Báo cáo y học: " Identification of a novel motif responsible for the distinctive transforming activity of human T-cell leukemia virus (HTLV) type 1 Tax1 protein from HTLV-2 Tax2" pps

Báo cáo y học: " Identification of a novel motif responsible for the distinctive transforming activity of human T-cell leukemia virus (HTLV) type 1 Tax1 protein from HTLV-2 Tax2" pps

... ዧዲዬየዧዮየዡዡየየድዧዩዢየዤደዣዝዲዥዲየዬዡዠ 19 8-207 18 5 -19 1 18 5-207 60 Tax207 C) 80 Tax1 (19 8-207) * ** Tax1 (18 5 -19 1) * Tax1 Transformation (Number) 10 0 Tax1 (18 5-207) B) Tax 40 20 Tax1 Tax1 (18 5-207) Tax1 (18 5 -19 1) Tax1 (19 8-207) ... 225- 232 C) 60 Tax1 Transformation (Number) 80 40 Tax300 * Tax1( 2 31 - 232 ) * Tax1(225-227) * Tax1(225- 232 ) 10 0 B) Tax 20 Tax1 Tax1 Tax1 Tax1 Tax300 (225- 232 ) (225-227) ( 2 31 - 232 ) Figure Tax1(225- 232 ) ... 2 31 - 232 Tax300 2 31 - 232 225-227 225- 232 Control Tax1 Tax300 225-227 2 31 - 232 C) 225- 232 Control Tax1 Tax300 2 31 - 232 225-227 225- 232 Tax1 B) Control 225- 232 p100 p100 p52 Tax p52 Tubulin Tax Sp1 Tubulin...

Ngày tải lên: 12/08/2014, 23:22

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Báo cáo y học: "inferring steady state single-cell gene expression distributions from analysis of mesoscopic samples" docx

Báo cáo y học: "inferring steady state single-cell gene expression distributions from analysis of mesoscopic samples" docx

... -1. 208 535 × 10 9 GAPDH 0.6946698 2 .12 24 43 × 10 8 -4.484740 × 10 7 0.9 838 246 1 .37 08 01 × 10 6 -2.4 416 42 × 10 5 8.885468 × 1 03 -1. 719 060 × 1 03 4.000586 × 10 7 -7.7 230 61 × 10 6 3. 65 617 6 × 10 6 -6.59 13 7 0 × 10 5 -2.590 916 ... -2.590 916 × 10 10 -2. 015 157 × 10 9 DDR1 PIK3 PNN ZCCHC7 0. 914 832 9 0. 916 034 8 0.982 710 1 0 .11 49602 ATP5L 0.67 930 07 1. 4645 13 × 10 9 -2 .12 7757 × 10 8 GNAS 0.8224466 2.762874 × 10 7 -5.59 630 1 × 10 6 λ and ... f 1x10 cells 10 s a m p l e s o f 1x1 1x10 cells 10 s a m p l e s o f 1x1 1x10 cells (b) 12 0 m g o f RN A plat e of ~1x107 ce ll ~1x1 cells s 10 d il u t i on s f r o m RN A o f 1x1 cells 1x10...

Ngày tải lên: 14/08/2014, 17:22

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Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt

Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt

... c1 2,4-Pentanedione 1 1 ,1- Difluoro-2,4-pentanedione · 10 )3 8.2 1, 1 ,1- Trifluoro-2,4-pentanedione · 10 )4 NM 1- Phenyl -1 ,3- butanedione · 10 )2 0 .3 4,4-Difluoro -1- phenyl -1 ,3- butanedione Structure · 10 )4 ... 3. 04 3. 13 4.06 4 . 31 0.0 13 0.008a 0. 012 b 0. 012 b 0. 016 c 0. 016 c )5.65 35 . 61 0.2207 )6.57 16 .65 0.0 13 4 Glu 2.04 3. 31 4.28 3. 83 0.008a 0. 012 b 0. 012 b 0. 012 b H2O a,b,c O C O C O 2.08 0.008a The Debye-Waller ... Soc 12 6, 12 202 12 2 03 35 Straganz GD & Nidetzky B (2005) Reaction coordinate analysis for b-diketone cleavage by the non-heme Fe2+-dependent dioxygenase Dke1 J Am Chem Soc 12 7, 1 230 6 1 2 31 4 36 Bugg...

Ngày tải lên: 18/02/2014, 06:20

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Tài liệu Báo cáo khoa học: Structure of the putative 32 kDa myrosinase-binding protein from Arabidopsis (At3g16450.1) determined by SAIL-NMR docx

Tài liệu Báo cáo khoa học: Structure of the putative 32 kDa myrosinase-binding protein from Arabidopsis (At3g16450.1) determined by SAIL-NMR docx

... MBPfromB.napus1 -12 5 MBPfromB.napus194 -33 6 MBPfromB.napus356-498 At1g52 030 .2 -15 4 At1g52 030 .16 1-289 At1g52 030 .33 6-476 At3g16400.2 -14 2 At3g16440.2 -14 4 At3g16440 .15 4 -30 0 At3g16470.2 -14 5 At3g16470 .15 8-297 At3g16470 .30 8-450 ... At1g52 030 .2 -15 4 At1g52 030 .16 1-289 At1g52 030 .4 91- 634 At3g16400.2 -14 2 At3g16440.2 -14 4 At3g16440 .15 4 -30 0 At3g16470.2 -14 5 At3g16470 .15 8-297 At3g16470 .30 8-450 At3g2 13 8 0.7- 13 0 LG FEEFEIDYPSEYITAVEGTYDKIFG SDGLIITMLRFKTNK-QTSAPFG ... GalNAca1 -3( Fuca1-2) Galb1 -3( Fuca1-4)GlcNAcb1-3Galb1-4Glc PA sugars that did not show affinity for At3g16450 .1 Galb1 -3( Fuca1-4)GlcNAcb1-3Galb1-4Glc Galb1-4(Fuca1 -3) GlcNAcb1-3Galb1-4Glc (GlcNAcb1-4GlcNAc )3 Chitohexaose...

Ngày tải lên: 18/02/2014, 14:20

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Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

... structure topology cartoons Bioinformatics 19 , 31 1 31 2 35 Bergmeyer HU (19 74) In Methods of Enzymatic Analysis, Vol 1, 2nd edn (Bergmeyer HU, ed.), pp 11 2 11 7 Verlag Chemie, Weinheim ⁄ Academic Press, ... residues Ile18, Ile149, Leu172, Phe179 and the aromatic ring of Tyr194 The side chains of Tyr194, Asn150 and Asp108 form a hydrophilic patch close to the zinc ion and to the His 110 ⁄ Asp 112 pair ... atom of His12, one of the Od atoms of Asp15 and the Ne atom of His1 13 His1 13 protrudes from helix a4 whereas His12 and Asp15 both belong to the loop which joins the b1strand with the a1-helix and...

Ngày tải lên: 19/02/2014, 00:20

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Tài liệu Báo cáo khoa học: Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt

Tài liệu Báo cáo khoa học: Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt

... CRPW13F CRPW13F(cAMP)2 CRPW13F(cAMP)4 CRPW13FICAP CRPW13Flac CRPW13Fgal 5. 83 5.59 5.99 5.55 5.85 5.85 4.42 1. 55 1. 54 2 .10 2. 23 2 .17 24.2 72 .3 74 .3 62.2 61. 9 62.9 ặRổ (A) ặEổ (%) 0.50 0 . 31 ... CRPW85A CRPW13F(cAMP)2 CRPW85A(cAMP)2 20.5 15 .3 22 .1 10.45 v2 r(0) 2.4 1. 8 6.9 3. 0 0. 23 0. 21 0. 23 0. 23 0.02 0.02 0. 03 0. 03 1. 222 1. 055 1. 036 1. 146 supported recently by NMR studies [7]; ... used: 14 650 )1 )1 M ặcm at 259 nm for cAMP [16 ] and 6000 M )1 cm )1 at ể FEBS 20 03 Conformational changes induced by cAMP and DNA binding to CRP (Eur J Biochem 270) 14 15 34 0 nm for AEDANS [18 ] Absorption...

Ngày tải lên: 20/02/2014, 23:20

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Tài liệu Báo cáo Y học: Functional analysis of a small heat shock/a-crystallin protein from Artemia franciscana docx

Tài liệu Báo cáo Y học: Functional analysis of a small heat shock/a-crystallin protein from Artemia franciscana docx

... p26-full None p26-ND36 1 36 p26-ND60 1 60 p26-CD40 1 53 19 2 p26-CD10 1 83 19 2 p26-alpha 1 60 and 1 53 19 2 Designations Primer sequences (p26-1Bam-s) (p26 -19 2Xho-as) (p26 -36 Bam-s) (p26 -19 2Xho-as) (p26-60Bam-s) ... Differential protective activity of aA- and 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 aB-crystallin in lens epithelial cells J Biol Chem 275, 36 8 23 36 8 31 Samali, A., Robertson, J.D., Peterson, ... H & de Jong, W.W (19 96) Immobilization Ó FEBS 2002 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 Small heat shock/a-crystallin protein from Artemia (Eur J Biochem 269) 9 41 of the C-terminal...

Ngày tải lên: 22/02/2014, 04:20

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Báo cáo khoa học: Global shape and pH stability of ovorubin, an oligomeric protein from the eggs of Pomacea canaliculata pot

Báo cáo khoa học: Global shape and pH stability of ovorubin, an oligomeric protein from the eggs of Pomacea canaliculata pot

... Nature 16 0, 33 3 33 4 Cheesman DF (19 58) Ovorubin, a chromoprotein from the eggs of the gastropod mollusk Pomacea canaliculata Proc R Soc Lond [Biol] 14 9, 5 71 587 Garı´ n CF, Heras H & Pollero RJ (19 96) ... Zool 276, 30 7 31 4 Dreon MS, Heras H & Pollero RJ (20 03) Metabolism of ovorubin, the major egg lipoprotein from the apple snail Mol Cell Biochem 2 43, 9 14 10 Palozza P & Krinsky NI (19 92) Astaxanthin ... cryptic? Nature 286, 14 9 15 0 29 Snyder NFR & Snyder HA (19 71) Defenses of the Florida apple snail Pomacea paludosa Behaviour 40, 17 5– 215 30 Randall D, Burggren W & French K (19 97) Energy acquisition:...

Ngày tải lên: 07/03/2014, 06:20

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Báo cáo khoa học: Solution structure of the catalytic domain of RICH protein from goldfish pot

Báo cáo khoa học: Solution structure of the catalytic domain of RICH protein from goldfish pot

... V 332 A 319 G 335 T322 T 236 38 0 34 0 30 0 260 220 18 0 0 .1 Residue Number Δchem shift (p.p.m.) C T 236 0.5 V 332 V 332 G 335 0 .3 T322 T 236 38 0 34 0 30 0 260 220 18 0 0 .1 Residue Number E F D2 41 0 .3 F 239 D2 41 ... regions 17 89 664 502 222 79 32 2 19 0 .1 7 .1 91. 4 9 .1 39 .6 34 .0 9.0 ± ± ± ± ± ± ± 3. 0 0 .3 3.0 0.4 1. 4 2.0 1. 7 0.0 014 ± 0.00 01 0 .30 ± 0. 01 0 .18 ± 0. 01 0. 017 ± 0.0 01 0.48 ± 0.05 0. 61 ± 0.05 1. 15 ± 0.07 ... for Thr322 (0.67 p.p.m.), Thr 236 (0.60 p.p.m.), Asp2 41 (0 .36 p.p.m.), Val 332 (0 .35 p.p.m.), Gly 335 (0.27 p.p.m.), Phe 239 (0.27 p.p.m.) and Ala 319 (0.27 p.p.m.) (Fig 5A) Thr 236 and Thr322 are...

Ngày tải lên: 07/03/2014, 10:20

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Báo cáo khoa học: The Rieske protein from Paracoccus denitrificans is inserted into the cytoplasmic membrane by the twin-arginine translocase doc

Báo cáo khoa học: The Rieske protein from Paracoccus denitrificans is inserted into the cytoplasmic membrane by the twin-arginine translocase doc

... activity (%) fbc::Km deletion strain Complemented wild-type R15K R16K R15K ⁄ R16K Y20K C152S 4 018 13 9 7 67 29 929 12

Ngày tải lên: 07/03/2014, 11:20

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Báo cáo khoa học: Structural and functional roles for b-strand 7 in the a-crystallin domain of p26, a polydisperse small heat shock protein from Artemia franciscana pdf

Báo cáo khoa học: Structural and functional roles for b-strand 7 in the a-crystallin domain of p26, a polydisperse small heat shock protein from Artemia franciscana pdf

... R 110 G F 112 R R 114 A Y 116 D WT 25.4 25.5 25.4 25.5 25.5 29–4 43 29 15 0 29–500 29–4 43 29–669 17 20 17 26 COS -1 cells R 110 G F 112 R R 114 A Y 116 D WT 20.7 20.8 20.7 20.8 20.8 14 .2–4 43 14 .2 15 0 14 .2–500 14 .2–4 43 ... R 110 G F 112 R R 114 A Y 116 D (E coli) (Artemia) (%) (%) (%) (%) (%) (%) a-helix b-antiparallel b-parallel b-turn Random coil 20 .3 18 .0 10 .2 16 .7 34 .8 20.2 18 .2 10 .2 16 .6 34 .8 25.7 12 .9 9.7 16 .9 34 .9 ... 16 .9 34 .9 19 .3 20.5 10 .1 16.9 33 .3 17 .7 21. 6 10 .3 16 .5 34 .0 17 .7 23. 0 10 .0 16 .8 32 .5 Discussion Fig Secondary structure of p26 Far-UV CD spectra were obtained for purified p26 dissolved in 10 mM NaH2PO4,...

Ngày tải lên: 07/03/2014, 12:20

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Báo cáo khoa học: Solution structure of the active-centre mutant I14A of the histidinecontaining phosphocarrier protein from Staphylococcus carnosus ppt

Báo cáo khoa học: Solution structure of the active-centre mutant I14A of the histidinecontaining phosphocarrier protein from Staphylococcus carnosus ppt

... regions 83. 7% 13 . 5% 1. 7% 1. 1% Energies of the 10 selected structures after refinement in water E/kJÆmol )1 Etotal ENOE ) 13 5 34 ± 30 6 13 7 ± 11 0 .10 2 4 818 A Moglich et al (Eur J Biochem 2 71) ¨ the ... Biochem 2 71) ¨ 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 1H NMR study of histidine containing protein (HPr) from Staphylococcus carnosus at high pressure Prot Sci 9, 6 93 7 03 Landt, ... restraints per residue 0.088 0. 13 0 77.5 16 .5 4.7 1 .3 Energies of the 10 selected structures after refinement in water E/kJÆmol )1 Etotal ENOE ) 11 752 ± 456 485 ± 21 .3 0.0 71 0 .11 2 mutant protein Compared...

Ngày tải lên: 07/03/2014, 16:20

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Báo cáo khoa học: Fluorescence quenching and kinetic studies of conformational changes induced by DNA and cAMP binding to cAMP receptor protein from Escherichia coli ppt

Báo cáo khoa học: Fluorescence quenching and kinetic studies of conformational changes induced by DNA and cAMP binding to cAMP receptor protein from Escherichia coli ppt

... 4. 23 3. 93 f1 sm (ns) kq1 (M ặs ) x 10 )1 kq2 (M )1 s )1) x 10 )1 kq1 (M )1 s )1) x 10 )1 0.69 0.65 0.59 0.57 0. 51 0.58 0.55 0.29 0.62 0.62 2 . 31 2 .14 1. 62 2.68 2 . 31 4 .16 4.67 4.60 2.07 2 .17 0. 73 1. 23 ... CRPW85Agal )1 )1 KSV1 (M ) KSV2 (M ) V (M ) f1 kmaks1 (nm) kmaks2 (nm) 9. 61 10.08 7.45 5.54 5.02 1. 69 2.85 0.00 0.00 0.00 0.55 0.57 0.80 0.78 0. 81 35 0 34 4 34 5 34 6 34 3 33 8 33 8 33 8 34 0 33 7 ... parameters Complex koff (s )1) kon (s )1 M )1) ã 10 6 Ka (M )1) ã 10 5 CRPwtICAPF CRPwtlacF CRPwtgalF 5.8 0.6 8.5 0.9 5 .1 0.9 3. 4 0.2 1. 1 0.2 2.4 0.2 5.9 0.9 1. 2 0 .3 4.7 0.9 11 10 )1 Acrylamide quenching...

Ngày tải lên: 07/03/2014, 16:20

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Báo cáo khoa học: Pulchellin, a highly toxic type 2 ribosome-inactivating protein from Abrus pulchellus Cloning, heterologous expression of A-chain and structural studies ppt

Báo cáo khoa học: Pulchellin, a highly toxic type 2 ribosome-inactivating protein from Abrus pulchellus Cloning, heterologous expression of A-chain and structural studies ppt

... (Tyr74, Tyr1 13 , Glu164, Arg167 and Trp198 in abrin-a and abrin-c, and Tyr 115 , Tyr158, Glu 212 , Arg 215 and Trp246 in ricin) and another ve conserved residues (Asn72, Arg124, Gln160, Glu195 and Asn196 ... DNA Seq 14 , 31 13 1 7 33 Lord JM (19 85) Precursors of ricin and Ricinus communis agglutinin Glycosylation and processing during synthesis and intracellular transport Eur J Biochem 14 6, 411 416 34 Frigerio ... saporin-L1: effect on DNA, RNA and poly(A) Biochem J 31 9, 5075 13 10 Barbieri L, Battelli MG & Stirpe F (19 93) Ribosomeinactivating proteins from plant Biochim Biophys Acta 11 54, 237 287 11 Ferreras...

Ngày tải lên: 07/03/2014, 16:20

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Báo cáo khoa học: A DmpA-homologous protein from Pseudomonas sp. is a dipeptidase specific for b-alanyl dipeptides Hidenobu Komeda and Yasuhisa Asano docx

Báo cáo khoa học: A DmpA-homologous protein from Pseudomonas sp. is a dipeptidase specific for b-alanyl dipeptides Hidenobu Komeda and Yasuhisa Asano docx

... 45 38 36 69 64 45 44 43 79 73 73 70 46 73 66 41 37 74 65 63 51 50 PP 534 1 PA1 410 PP3845 PA2 711 PA1486 PP3844 PLU2258 Atu5242 DmpA PSTPO 13 4 3 PP02 03 PP3847 PA3599 PA25 91 PP3846 PA3598 PP 038 2 SMc 019 62 ... KT2440 E coliK -12 S flexneri 30 1 Q88C42 A 834 70 Q88G80 G 833 06 G 834 60 Q88G 81 Q7N4R4 AE 318 9 Q59 632 Q887F3 Q88RC9 Q88G78 A 8 31 96 H 833 20 Q88G29 H 8 31 95 Q88QV2 Q92MW3 Q88G80 A 833 21 Q88HL0 C 911 7E Q83J77 FEBS ... 216 40 2970 18 10 2090 11 60 30 6 0.54 1 .38 5.69 5 .37 7.65 10 0 60.9 70.4 39 .1 10 .3 b-d-thiogalactopyranoside for 15 h at 37 °C, exhibited no hydrolytic activity toward d-Ala-pNA When E coli JM109...

Ngày tải lên: 07/03/2014, 21:20

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Báo cáo khoa học: GHP, a new c-type green heme protein from Halochromatium salexigens and other proteobacteria potx

Báo cáo khoa học: GHP, a new c-type green heme protein from Halochromatium salexigens and other proteobacteria potx

... 2547.8b 2 13 4 .8a Sequence 14 78.8 2850 .3 2979 .3 311 0 .3 960.4 (2855.2) 16 68.7 10 02.5 10 25.5 1 2 31 .5 16 12.7 19 43. 9 24 01. 1 2289 .1 2746 .3 (502.2) 13 2 1. 7 9 01. 4 8 31 .3 19 89.8 19 41. 9 411 0 .1 39 81. 0 38 52.0 ... 2805.8 2676.7 2547.7 2 13 5 .0 29– 41 3 28 2–28 1 28 66–72 42–65 11 –25 26 33 34 –42 64–72d 34 –47 57–72 53 72 34 –52 34 –56 45–48 22 31 35 –42e 67–72 32 –48 32 –48 1 31 2 31 3 31 1 21 2– 21 3 21 49–66 c MALDI ... (Da) b12 13 4 3.7 800 Cys-SO2H Arg Met b10 b 11 1208.7 12 77.7 10 56 Mass (Da) Cys-SO3H + Glu Gln Asn Leu Glu 12 51. 7 10 77.6 b15 1 7 31 .2 b16 18 44 .3 10 0 b9 b8 9 21. 7 822.6 b 13 b 13 14 89 .1 b14 b 11 1208.9 %...

Ngày tải lên: 08/03/2014, 08:20

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