2 inhibition of inflammatory cascade by mesenchymal stem cells

... Treatment of Femoral Head Necrosis with Arterial Perfusion of Marrow Stem Cells Chin J Integr Trad West Med 2004,24(11):999-1002 Yan Z, Hang D, Guo C, Chen Z Fate of mesenchymal stem cells transplanted ... improvement of blood circulation in the necrotic femoral head and surrounding tissues [4] Mesenchymal stem cells (MSCs) are multipotent stem cells that can differentiate into a variety of cell types ... infusion of bone marrow-derived Flk-1+CD31-CD34- mesenchymal stem cells: feasibility and safety from monkey to human Stem Cells Dev,2006,15(3):349-357 Devine SM, Bartholomew AM, Mahmud N, et al Mesenchymal...

Ngày tải lên: 25/10/2012, 11:18

... circumstance, the treatment of MI by using MSCs showed poor survival of transplanted cells In addition to the quick loss of cells within 24 h of transplantation caused by cell leakage into the extra ... Adv-HO-1, and strong expression of GFP was observed by fluorescence analysis (Fig 1A) The over-expression Page of of HO-1 was confirmed by Western blotting (Fig 1B) Levels of HO-1 in HO-1-MSCs were ... nonadherent cells were removed by a medium change at 72 h and every four days thereafter After two passages, homogeneous MSCs that devoid of hematopoietic cells were used A total of × 106 cells/ ml...

Ngày tải lên: 10/08/2014, 05:21

... with Regard to the Source of Fetus-Derived Stem Cells Stem cells 2007, 25:2886-2895 Wu KH, Mo XM, Zhou B, Lu SH, Yang SG, Liu YL, Han ZC: Cardiac potential of stem cells from whole human umbilical ... sheets from differentiated human umbilical cord mesenchymal stem cells We assessed viability of the cells of sheets at room temperature No indication of cell apoptosis was noticed from the PNIPAAm ... proofreading of the manuscript This work was supported by The Major Project of Department of Science &Technolgoy of Hainan Province, P R of China.(No 20061003) Author details Department of Neurology,...

Ngày tải lên: 10/08/2014, 05:21

... osteogenesis and vascularization of mesenchymal stem cells Cells Tissues Organs 2009, 190:185-193 Rodbell M: The metabolism of isolated fat cells IV Regulation of release of protein by lipolytic hormones ... DR: Multilineage potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Pittenger MF, Mosca JD, McIntosh KR: Human mesenchymal stem cells: progenitor cells for cartilage, bone, ... before [24] Most cells were in resting period of cell cycle agreeing with the characteristics of human bone marrow-derived mesenchymal stem cells [5] The Yang et al Journal of Biomedical Science...

Ngày tải lên: 10/08/2014, 10:20

... critical target for the treatment of inflammatory arthritis [11] Mesenchymal stem cells (MSCs) are cells of stromal origin that can exert profound immunosuppression by modulating T and B cell proliferation ... Page of 13 the activation of autoreactive Th1 cells [37,38] Downregulation of the inflammatory Th1 and the elevated IL-10 levels by UC-MSCs prompted us to further investigate the effect of Tregs ... Yao R, Tuan RS: Comparison of proliferative and multilineage differentiation potential of human mesenchymal stem cells derived from umbilical cord and bone marrow Stem Cells 2007, 25:1384-1392...

Ngày tải lên: 12/08/2014, 15:22

... Macrophages and other inflammatory cells, cells of mesenchymal origin Chemotactic effect on other inflammatory cells, stimulation of extracellular matrix synthesis, angiogenesis, recruitment of endogenous ... Targeted cells: MSCs, osteoprogenitor cells, osteoblasts, chondrocytes Mitogenic for mesenchymal cells and osteoblasts, chemotactic for inflammatory and mesenchymal cells Targeted cells: mesenchymal ... Types of cells used in bone tissue engineering for osteogenic differentiation 59 2.3.1 Potential of mesenchymal stem cells (MSCs) for bone healing 60 2.3.2 Potential of bone marrow derived mesenchymal...

Ngày tải lên: 11/09/2015, 09:57

... differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human mesenchymal stem ... arcades of Benninghoff [29] and by the appearance of hypertrophic cells Analyses of lacunae surface areas in the different growth plate zones revealed mean lacunae surface areas ± SD of 100.8 ... zone of cartilage could be clearly separated by the proximity of the cells to the joint space and the bone Page 10 of 15 (page number not for citation purposes) respectively, alignment of the...

Ngày tải lên: 09/08/2014, 14:22

... DR (1999) Multilineage potential of adult human mesenchymal stem cells Science 284, 143–147 Porada CD, Zanjani ED & Almeida-Porad G (2006) Adult mesenchymal stem cells: a pluripotent population ... Kitamura S & Nagaya N (2007) Effect of hypoxia on gene expression of bone marrowderived mesenchymal stem cells and mononuclear cells Stem Cells 25, 1166–1177 Li L, Zhang S, Zhang Y, Yu B, Xu Y & Guan ... antifibrotic effect of transplanted mesenchymal stem cells in a rat model of global heart failure Mol Biol Rep 36, 725–731 Li L, Zhang Y, Li Y, Yu B, Xu Y, Zhao S & Guan Z (2008) Mesenchymal stem cell...

Ngày tải lên: 18/02/2014, 04:20

... elan-immunoreactive material Fig Evolution of rec-trappin-2 production by Pichia pastoris as a function of the duration of fermentation Aliquots of concentrated supernatants of rec-trappin-2-secreting P pastoris ... before measurement of the residual enzymatic activity by addition of 10 lL of a 100 mM Suc(Ala)3-p-NA stock solution Product release was continuously recorded until a constant rate of paranitroaniline ... favor of proteases which widely overwhelm the inhibitory capacity of lung uid The biological signicance of this control mechanism has been highlighted by the observation that the development of...

Ngày tải lên: 07/03/2014, 15:20

... supported in part by the Natural Science Foundation of Fujian Province of China (2008J0282) and by the professorial academic Foundation of Fujian Medical University (JS06077) Page of 10 Author details ... direct inhibitor of caspase-3 and Biochemistry 2001, 40:1117-1123 Zhu W, Chen J, Cong X, Hu S, Chen X: Hypoxia and serum deprivationinduced apoptosis in mesenchymal stem cells Stem Cells 2006, 24:416-425 ... mesenchymal stem cells modified by Survivin on experimental stroke in rats Journal of Translational Medicine 2011 9:105 Submit your next manuscript to BioMed Central and take full advantage of: ...

Ngày tải lên: 18/06/2014, 19:20

... supported in part by the Natural Science Foundation of Fujian Province of China (2008J0282) and by the professorial academic Foundation of Fujian Medical University (JS06077) Page of 10 Author details ... direct inhibitor of caspase-3 and Biochemistry 2001, 40:1117-1123 Zhu W, Chen J, Cong X, Hu S, Chen X: Hypoxia and serum deprivationinduced apoptosis in mesenchymal stem cells Stem Cells 2006, 24:416-425 ... mesenchymal stem cells modified by Survivin on experimental stroke in rats Journal of Translational Medicine 2011 9:105 Submit your next manuscript to BioMed Central and take full advantage of: ...

Ngày tải lên: 20/06/2014, 04:20

... and Use of Laboratory Animals, as published by the National Academy Press (NIH Publication No 85-23, revised 1996) Page of 10 70%-80% of confluence, they were trypsinized by the addition of 0.25% ... manufacturer’s instructions Cells in which the nucleus was stained brown were defined as TUNEL-positive and the percentage of apoptotic cells per total number of cells was determined by two independent ... were made by one-way ANOVA, followed by Scheffe’ multiple comparison test Comparisons of the time course of the LDPI index were made by 2-way ANOVA for repeated measures, followed by Scheffe’...

Ngày tải lên: 10/08/2014, 05:21

... examined by supershift assay using antibodies for the p50 or p65 components of NF-κB (data not shown) One of the consequences of inhibition of NF-κB is the inhibition of the nuclear translocation of ... system The relative density of the protein bands was scanned by densitometry using MyImage (SLB, Seoul, Korea), and quantified by Labworks 4.0 software (UVP Inc., Upland, California) Immunofluorescence ... effect of melittin and bee venom on the generation of NO induced by LPS or SNP JNK inhibitor suppressed the inhibitory effect of melittin and bee venom on the generation of NO induced by LPS...

Ngày tải lên: 11/08/2014, 08:22

... intensities of the appropriate bands in the IFN-treated cells, as a percentage of the values seen in the absence of IFNa treatment Examination of the levels of procaspase-8 in MCF-7 cells by immunoblotting ... BID t-BID TRAIL (ng/ml) Fig Effects of IFNa on the sensitivity of MCF-7 and HeLa cells to inhibition of protein synthesis by TRAIL MCF-7 cells (A) and HeLa cells (B) were cultured for 72 and 24 ... Effects of IFNa treatment on the sensitivity of cells to inhibition of protein synthesis by TRAIL We have previously shown that protein synthesis is rapidly downregulated following exposure of cells...

Ngày tải lên: 19/02/2014, 06:20

... the presence of DA and TIQs Data are shown as the percentage of the activity level in the absence of inhibitors (n = 4) (B) A Lineweaver–Burk plot of hTH activity in the presence of NMNorsal (1.0 ... and spin of the iron in hTH in the presence of DA and four structurally distinct TIQs The divalent iron of the unbound enzyme was EPRsilent Addition of DA produced a signal with g values of 7.1 ... Oxidation of the active site iron (A) Rapid freeze-quench EPR spectra of hTH in the presence of equimolar concentrations of DA or NMNorsal exhibited a characteristic signal at g values of 7.1 and...

Ngày tải lên: 07/03/2014, 05:20

... effect of targeted RNA in HeLa cells to be at the level of approximately 78% when 100 nm siDNA was used (Fig 2) Application of higher concentrations of siDNAs than of siRNAs is necessitated by the ... phases of RNAi Ago proteins are key components of RISC responsible for the cleavage of the target RNA The cleavage is catalysed by a Piwi domain of Ago proteins, the structural homolog of RNase ... Balzergue S (1999) Activation of systemic acquired silencing by localised introduction of DNA Curr Biol 9, 59–66 Voinnet O, Vain P, Angell S & Bulcombe DC (1998) Systemic spread of sequence-specific transgene...

Ngày tải lên: 16/03/2014, 02:20

... hydrolysis of BTC by this enzyme obeyed Michaelis–Menten kinetics In order to investigate the kinetics of this mutant more closely, we measured the hydrolysis of BTC catalyzed by the W82A mutant, by ... concentration of the product was formed The stock solutions of the two enzymes were prepared by dilution of the aliquots with the same amount of buffer The mixture was prepared by mixing together ... Progress curves for the hydrolysis of butyrylthiocholine catalyzed by the horse butyrylcholinesterase, by the W82A mutant of human butyrylcholinesterase and by the enzyme mixture Measurements...

Ngày tải lên: 24/03/2014, 03:21

... Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta Stem Cells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation of mesenchymal ... evidence of transdifferentiation by MET in the process of hepatic differentiation of AT-MSCs No previous report has demonstrated evidence of transdifferentiation of committed adult stem cells In ... system will provide a valuable tool, in addition to primary hepatocytes, for study of the molecular basis of the regenerative and developmental processes of hepatic cells in vitro Isolation of...

Ngày tải lên: 30/03/2014, 04:20

... new potential source of pluripotent cells for regenerative medicine The identification of niches of tissue-specific stem cells capable of replacing damaged differentiated cells in the hFTs may ... Prockop D: Mesenchymal stem/ multipotent stromal cells: the state of transdifferentiation and modes of tissue repair – current views Stem Cells 2007, 25:2896-28902 Valtieri M, Sorrentino A: The mesenchymal ... Vainzof M, Zatz M: SJL dystrophic mice express a significant amount of human muscle proteins following systemic delivery of human adipose-derived stromal cells without immunosuppression Stem Cells...

Ngày tải lên: 18/06/2014, 15:20

... marrowderived MSC is the major source of stem cells in these studies [22,24-27], the therapeutic role of adiposederived mesenchymal stem cells (ADMSCs) in acute IR injury of the lung has not been investigated ... endotoxin-induced systemic response by bone marrow-derived mesenchymal stem cells in mice Am J Physiol Lung Cell Mol Physiol 2007, 293:L131-141 23 Salgar S, Manning E, Ruiz P, Pham S: Mesenchymal Stem Cell ... was then determined by summation of all numbers divided by Immunofluorescent (IF) Studies and Crowded Score of Lung Parenchyma IF staining was performed for the examinations of CD68 (macrophage...

Ngày tải lên: 18/06/2014, 22:20