observed inhibition of neutrophil activation by surfactant with sp b c

... AGA GCG CAA GAG CCA GTC AA-3Â, restriction site XhoI This primer fuses the elan mature protein immediately downstream of < /b> the a-peptide sequence Primer 3; 5Â-CGAGCGGCCGCCCCTC TCA CTG GGG AAC-3Â, ... its complex with < /b> rec-elan Complexes were rst formed by < /b> incubating equimolar concentrations (10)6 M) of < /b> enzyme and inhibitor Dissociation of < /b> the complexes was induced by < /b> dilution in a concentrated ... nonreducing conditions (b) or reducing conditions ( +b) Molecular masses of < /b> the protein standards are shown on the left Fig Inhibition < /b> of < /b> the enzyme activity of < /b> pancreatic elastase by < /b> recelan Constant...

Ngày tải lên: 07/03/2014, 15:20

... Formation of < /b> a TH–Fe(III)–catecholamine complex induces an absorbance change at 700 nm that can be detected using visible spectroscopy Under anaerobic conditions, the absorbance change is only 50% of < /b> ... 2A) A kinetic analysis indicated that NMNorsal reduced hTH activity by < /b> competing with < /b> the essential pterin cofactor (Fig 2B) ; hTH inhibition < /b> by < /b> NMNorsal was noncompetitive with < /b> respect to tyrosine ... inhibition < /b> by < /b> NMNorsal or Norsal (n = 4) tetrahydropterin cofactor and an almost irreversible blockade of < /b> TH activity by < /b> facilitating oxidation of < /b> the catalytic iron [21,23] As catecholamine-bound...

Ngày tải lên: 07/03/2014, 05:20

... (Eur J Biochem 269) Ó FEBS 2002 Fig Dependence of < /b> the activity of < /b> various human BuChEs and horse BuChE on the concentration of < /b> TEA at various butyrylthiocholine concentrations BTC concentrations ... performed with < /b> a constant dielectric constant (e ¼ 1) Electrostatic force was treated without cutoffs and van der Waals forces were ˚ calculated with < /b> the shift method with < /b> a cutoff of < /b> 10 A All ... Pathways of < /b> ligand clearance in acetylcholinesterase by < /b> multiple copy sampling J Mol Biol 298, 705–726 Cleland, W.W (1977) Determining the chemical mechanisms of < /b> enzyme-catalyzed reactions by < /b> kinetic...

Ngày tải lên: 24/03/2014, 03:21

... transcription by < /b> HBc protein would decrease the inhibitory effect of < /b> IFN-a on HBV replication pCMV-HBc (WT), pCMV-HBc (L60V), pCMVHBc (S87G), pCMV-HBc (I97L) and pCMV-Tag1 were transfected into Huh7 cells ... hot-spot mutants has no impact on inhibition < /b> of < /b> HBV replication by < /b> IFN-a, indicating MxA may be independent of < /b> inhibition < /b> of < /b> HBV DNA replication by < /b> IFN-a The clinical significance of < /b> the inhibitory ... by < /b> IFN-a In order to evaluate whether the inhibition < /b> of < /b> MxA transcription by < /b> HBc protein could decrease the 2.1 IFN A inhibitory effect on HBV replication induced by < /b> IFN-a, pU19-1.24HBV was co-transfected...

Ngày tải lên: 12/08/2014, 01:22

... subunits of < /b> active caspase-8 [25,26] Caspase-8 in turn is responsible for initiating a cascade of < /b> activation < /b> of < /b> effector caspases that ultimately leads to the multiple changes in cells characteristic ... characteristic of < /b> TRAIL-induced apoptosis [27] The process of < /b> activation < /b> of < /b> caspase-8, and the downstream consequences that arise from it, are blocked by < /b> the caspase-8-speci c peptide inhibitor zIle-GluThr-Asp-fluoromethyl ... functionally active isoforms, caspase-8 ⁄ a and caspase-8 ⁄ b J Biol Chem 272, 26953–26958 25 Sohn D, Schulze-Osthoff K & Janicke RU (2005) Caspase-8 can be activated by < /b> interchain proteolysis without...

Ngày tải lên: 19/02/2014, 06:20

... significant increase in scu-PA activation < /b> on U937 cells (Fig 3B, C) Plactin D affected a-thrombin-mediated scu-PA activation < /b> only slightly (Fig 3B) Hirudin, a speci c inhibitor of < /b> Dual modulation of < /b> ... including U937 cells Therefore, we examined the effects of < /b> cystatin, an inhibitor of < /b> DPP-I, on tcu-PA ⁄ T activation < /b> by < /b> U937 cells Cystatin effectively inhibited tcu-PA activation < /b> by < /b> U937 cells (Fig ... susceptible to activation < /b> by < /b> Xa Under physiological coagulation conditions, prothrombin activation < /b> is catalyzed by < /b> the prothrombinase complex (factor Xa in complex with < /b> factor Va, phospholipids and Ca2+)...

Ngày tải lên: 07/03/2014, 00:20

... infected tobacco leaves by < /b> RT-PCR, using primers TMV1 (5¢-GCCCAAGGTGAACT TTTCAA-3¢) and TMV2 (5¢-TAGCGCAATGGCATACA CTC-3¢) The sequence of < /b> as-DNA is 5¢-CAATACTGTCT TTCTGGCCTTC-3¢, and that of < /b> ... 5¢-ATAGGCG GGAATTTTGCATC-3¢ Anti-TMV siRNA was synthesized using the DNA templates 5¢-AAGGGACGAGCA TATGTACACCCTGTCTC-3¢ and 5¢-AAGTGTACATAT GCTCGTCCCCCTGTCTC-3¢ and a Silencer siRNA Construction ... 5¢-AAATGAGTTTTCCAGAGCAACCCTGTCTC-3¢; antisense strand, 5¢-AAGTTGCTCTGGAAAACTCATCCTG TCTC-3¢) and a Silencer siRNA Construction Kit (Ambion) Acknowledgements This work was supported by < /b> grants from...

Ngày tải lên: 16/03/2014, 02:20

... observe the speci c binding using ECP and RITC-labeled ECP by < /b> monitoring the level of < /b> ¯uorescence, but the change in ¯uorescence level caused by < /b> the competition of < /b> ECP and RITC-labeled ECP could ... of < /b> cell surface receptors for ECP We could not show the presence of < /b> a speci c high-af®nity binding site for ECP on cells by < /b> competition assay This is probably because the af®nity of < /b> ECP for binding ... The number of < /b> colonies was counted, and the area occupied by < /b> the colonies was evaluated by < /b> image scanning assisted by < /b> a computer Fluorescence microscopy ECP and bovine RNase A were labeled with...

Ngày tải lên: 17/03/2014, 17:20

... Therapy Vol 10 No Rockel et al containing the rat Col2a1 48-bp minimal enhancer (5'CTGTGAATCGGGCTCTGTATGCACTCGAGAAAAGCCCCATTCATGAGA-3'), described by < /b> Lefebvre and coworkers [29], was obtained from Invitrogen ... (compare Figure 7b and Figure 4b; P < 0.001) To determine the effect of < /b> NF- B activation < /b> by < /b> caMEKK1 on RAR function, cells were co-transfected with < /b> caMEKK1 and I B- 2N I B- 2N inhibits NF- B activation < /b> ... thereby suppressing NF- B binding to DNA This mechanism could contribute to the reduction in binding to DNA of < /b> the TNF-α-activated complex observed < /b> under conditions in which chondrocytes were subjected...

Ngày tải lên: 09/08/2014, 10:22

... Amplification primers for each specific mRNA were deduced from published sequences and included bcl2 (P1: 5’-GTTCCACCCGTTTTCA-3’, P2: 5’-GCGAGTCCTCATTCTGT-3’), bad (P1: 5’-CA AGCCTGGATAAACAC-3’, ... min, sections were incubated with < /b> target-purified rabbit primary antibodies, including BCL2 (Anaspec Inc., Fremont, CA, USA), BAD (Anaspec Inc., Fremont, CA, USA), CASPASE-3 (Anaspec Inc., Fremont, ... sac B: In tbx5 knockdown embryos, a string-like cardiac morphology was observed,< /b> with < /b> no looping formation Most of < /b> the cardiac sac was occupied by < /b> pericardial effusion C: No apoptosis was observed...

Ngày tải lên: 10/08/2014, 10:20

... double-sranded DNA of < /b> g RZ57 was synthesized with < /b> ApaΙ and HindIII protruding ends Their sequences are as follows: 5’ AGCTT GGGAC CACCA CCACG CGGAC GCAAG AAGGG CAAGC GGCAA CGCAA GGCAA AGGGACCC CCC ... CAAGC GGCAA CGCAA GGCAA AGGGACCC CCC 3’ and 5’ A CCCTG GTGGT GGTGC GCCTG GCTGG TCCCG TTCGC CGTTG CGTTC CGTTT CCCTG GG GGG 3’ The predicted secondary structure of < /b> g RZ57 are seen in Figure After ... generated by < /b> RT-PCR of < /b> a 184 bp fragment by < /b> of < /b> hTR cDNA by < /b> digestion of < /b> Recombinant plasmid pBBS212 containing hTR gene (provided Ribozyme transfected bel7402 cells and HCT116 cells showed decrease of...

Ngày tải lên: 10/08/2014, 10:20

... specific detection of < /b> neutrophils Effects of < /b> LIM on cardiac function The cardiac function has been analyzed by < /b> the thermodilution and conduction catheter technique As shown in Figure 1, the cardiac ... http://www.journal-inflammation.com/content/4/1/21 σ specific conductance of < /b> blood G(t) left ventricular conductance G(p) parallel conductance A pressure tip transducer in the conductance catheter measures left ventricular pressure ... global distribution of < /b> neutrophils within the body after passing the LIM, technetium-labeled neutrophils were injected into the blood circulation before onset of < /b> CPB or CPB with < /b> LIM (n = 2, each...

Ngày tải lên: 11/08/2014, 08:21

... in vitro, inhibition < /b> of < /b> the specific targets responsible for anti-HIV activity of < /b> Prunella in cells remains unclear Identification of < /b> constituents of < /b> P vulgaris that confer the inhibition < /b> to ... Cytotoxicity of < /b> fraction was in contrast to what we had observed < /b> with < /b> similar concentrations of < /b> aqueous extract suggesting that either cytotoxic compounds were being concentrated in this fraction ... size exclusion fractions (100 μg/mL) (A), ethanol-soluble sub-fractions (B) , ethanol-insoluble sub-fractions (C) , or a combination of < /b> both the ethanol soluble and insoluble fractions at concentrations...

Ngày tải lên: 12/08/2014, 04:21

... 104) B 35 30 25 20 15 10 HIV HIV-lhNef Luc-Nef + Figure Test of < /b> sequence-specificity of < /b> the inhibition < /b> by < /b> HIV-lhNef Test of < /b> sequence-specificity of < /b> the inhibition < /b> by < /b> HIV-lhNef (A) Trans -inhibition < /b> ... mononuclear cells by < /b> specific RNA interference Nucleic Acids Res 2002, 30:4830-4835 Anderson J, Banerjea A, Akkina R: Bispecific short hairpin siRNA constructs targeted to CD4, CXCR4, and CCR5 confer ... induce a potent HIV-1-specific RNA silencing response in cells Tests with < /b> reporter constructs not support a sequence-specific RNAi effect, although the effect induced by < /b> lhNef is HIV-1 specific...

Ngày tải lên: 13/08/2014, 09:20

... than 10% inhibition < /b> The specific effect of < /b> HEXIM1 expression was highlighted by < /b> the results shown in Fig 2B G5HIV-Luc reporter was activated by < /b> co-expression of < /b> a GAL4-TBP, and such activation < /b> ... absence of < /b> Tat [20,21] We sought to determine the consequences of < /b> ectopically expressed F:HEXIM1 on P-TEFb induced transcription in the absence of < /b> Tat We showed that direct recruitment of < /b> CyclinT1 ... for efficient Tat inhibition < /b> Results Tat activity is inhibited by < /b> HEXIM1 Tat activity involves direct interaction with < /b> CDK9/ CyclinT1 (P-TEFb) complex However, two major P-TEFbcontaining complexes...

Ngày tải lên: 13/08/2014, 09:21

... an electrochemical process, electrochemical techniques can be used for investigating its mechanistic details The suggested electrochemical mechanism of < /b> copper corrosion inhibition < /b> could be verified ... will become: Cu+ Cl- → CuCl0+ e- (2.4) CuCl0 + Cl- → CuCl2- (2.5) The dissolution process is controlled by < /b> the mass transfer of < /b> CuCl2- from the electrode surface to the bulk solution The reactions ... 2.2 Phosphate as Corrosion Inhibitor 2.2.1 The Application of < /b> Phosphates Phosphates can be applied as phosphoric acid, combinations of < /b> orthophosphoric acid, zinc orthophosphate and polyphosphate...

Ngày tải lên: 22/10/2015, 22:43

... virulence [15] On the other hand, the decay-accelerating factor (DAF) with < /b> its well-established role in the inhibition < /b> of < /b> activation < /b> of < /b> complement cascade [16] has recently been shown to be a co-receptor ... screening of < /b> cellular proteins interacting with < /b> HBsAg Figure Determination of < /b> Size of < /b> Inserts in cDNA Library Constructed from HBV-transfected HepG2 Cells The construction of < /b> this cDNA library from HBV-infected ... the ubiquitin complex This complex will then be recognized by < /b> UBPs and the TF that is located at the C terminus of < /b> Cub will be cleaved off and result in downstream reactions, which is the activation...

Ngày tải lên: 02/11/2012, 11:08

... Inhibitors of < /b> GSNOR 14 Inhibition < /b> of < /b> A549 cells by < /b> C2 using BrdU Incorporation 27 Inhibition < /b> of < /b> A549 cells by < /b> C3 using BrdU Incorporation 28 Inhibition < /b> of < /b> A549 cells by < /b> C3 with < /b> ... 7.5 Dodecanoic acid 19 212 C1 78 93 1.3 C2 55 91 2.4 C3 75 95 1.1 Table In vitro Percent Inhibition < /b> and IC50 of < /b> C1 , C2 , C3 The percent inhibition < /b> was determined using conditions Inhibition < /b> studies ... REFERENCES 53 CURRICULUM VITAE vii LIST OF < /b> TABLES In vitro Percent Inhibition < /b> and IC50 of < /b> C1 , C2 , and C3 11 Compound Inhibition < /b> Comparison of < /b> ADHs 12 Treatments of < /b> RAW 264.7 cells...

Ngày tải lên: 24/08/2014, 10:34

... sequence of < /b> 500 bases from this vector, an inverted PCR was performed with < /b> primers (P3: 5¢-GAGAG TCAGCGCTGCTGTGCTACCCCAAGA-3¢, P4: 5¢-ACT TGGATGTTGTCGGGCGGCGTTGGCACA-3¢) The resulting PCR product ... (P1: 5¢-ACCG AGCCTCGAGGATATACCAAGGCAGAGGAGC-3¢, P2: 5¢-ACTTGCAGAGCGGCCGCTTGTCAGTTGGA GAAGC-3¢) Cloned cDNA was inserted into the XhoI– NotI site of < /b> the pCIneo vector (Clontech) In order to remove ... transfection into the HRA cell line The a1 microglobulin-bikunin cDNA was cloned by < /b> PCR using the human liver cDNA library (Clontech, Palo Alto, CA, USA) as a template with < /b> primers (P1: 5¢-ACCG AGCCTCGAGGATATACCAAGGCAGAGGAGC-3¢,...

Ngày tải lên: 17/03/2014, 11:20

... HSQC spectra of < /b> the reactions of < /b> a-Syn with < /b> quinone and DA (A) Overlay of < /b> the 1H-15N HSQC spectra of < /b> a-Syn (black) and the reaction product of < /b> a-Syn and Q (red) (B) Overlay of < /b> the 1H-15N HSQC spectra ... courses of < /b> the fibrillization processes of < /b> a-Syn incubated with < /b> the DA-analog compounds were monitored by < /b> a thioflavin (ThT) fluorescence assay [17] Figure displays inhibition < /b> of < /b> a-Syn fibrillization by < /b> ... (left) Table Molecular masses of < /b> the monomeric adducts as determined by < /b> electrospray mass spectrometry Molecular masses were of < /b> the peaks detected by < /b> ESI-MS Pattern shows incremental molecular masses...

Ngày tải lên: 23/03/2014, 15:20