1 cell sources for vascular tissue engineering

... medium to secrete ECM and form cell sheets, and the cell sheets were then wrapped around a temporary inert tubular support and allowed for maturation for some time to form a cylinder composed ... achieve improved systematic performance for the resultant construct Composite vascular graft constructions have been attempted in different approaches Acellular composite vascular grafts have been ... requirement for the structural or chemical properties of the components Therefore, choices of appropriate materials and techniques with fewer limitations are crucial for the development of composite vascular...

Ngày tải lên: 10/09/2015, 09:08

... pore size Small pores induce osteochondral formation before osteogenesis, whilst large pores that are well vascularized induce direct osteogenesis Tissue forms quickly on the outer surfaces of the ... thanks goes to Chris Lam for his guidance to develop my laboratory skills, Anand for his true support during the screw extrusion system development and maintenance, and Andrew for his valuable suggestions ... dispensing speed for 0.5 mm nozzle diameter 48 Figure 3.15 Theoretical and experimental porosity vs dispensing speed for different nozzle diameter 49 Figure 3.16 Modulus vs dispensing speed for different...

Ngày tải lên: 10/09/2015, 15:51

... functional cell source for cartilage repair Chondrocytes, fibroblasts, stem cells, and genetically modified cells have all been explored for their potential as viable cell sources for cartilage tissue ... numbers, 3000 cells, by centrifugation to form EBs In doing so, a reproducible and robust platform based on hESC differentiation through EB formation was set up for analysis of growth factor influence ... addition, biomaterials may also provide physical cues for cell orientation and spreading, which are critical for hESC differentiation and tissue formation For example, co-cultured hESC-derived chondrogenic...

Ngày tải lên: 11/09/2015, 09:57

... Ong Lee Lee and Chia Ser Min for their expertise in performing cell immunofluorescence staining; Drs Ying Lei and Cen Lian from the Department of Chemical Engineering for their guidance on bio-conjugation; ... material selection for liver tissue engineering 2-11 Scaffold fabrication for liver tissue engineering 2-16 Scaffold surface modification 2-20 Tissue culture for liver tissue ... role in contrast to vascular endothelial cells They perform special functions like filtration, regulation of sinusoidal blood flow and endocytosis [3] Kupffer cells account for about 10% of total...

Ngày tải lên: 12/09/2015, 10:22

... as temporary scaffolds for cell adhesion, guiding tissue formation while performing biochemical and biomechanical functions before the regenerative process is complete For example, bioresorbable ... biomaterial for bone tissue engineering, intended as a single-step cell expansion and in-situ osteogenic differentiation platform to be implemented as a non-invasive, injectable bone graft substitute for ... efficient tool for obtaining high yield mesenchymal stem cells (MSCs) isolation and expansion in-vitro • Establish the apatite-based porous microcarriers as an efficient platform for driving directed...

Ngày tải lên: 22/09/2015, 15:18

... bioreactor design I very much appreciate the efforts of Ms Julee Chan, senior lab officer in histology, for providing valuable information while performing histological analysis of the samples ... potential donor cell for our engineered ligament Generally, bone marrow and specific organs are the optimal cell sources for tissue engineering BMSCs have been extensively studied for bone, cartilage ... in a bioreactor ex vivo (in vitro) for a period of time before transplantation Special reactors are required to conduct biochemical reactions for the transformation and production of chemical...

Ngày tải lên: 04/10/2015, 10:25

... properties for appropriate applications Natural, ECM-derived proteins are apparently the first choice for soft TE, since they provide a physiologically relevant and recognizable platform for the ... with PBS, fixed using formalin for 20 minutes at RT and permeated using 0.1% Triton-X100 for minutes at RT Non-specific sites is blocked using 3% bovine serum albumin (BSA) for 90 minutes period ... the biomaterial aids in vivo revascularization as well as integration with the host tissue There are additional specific requirements for a biomaterial to be applied for CTE It includes that the...

Ngày tải lên: 05/10/2015, 19:04

... like to thank her for reviewing the drafts  Dr Simon Cool (IMB), project mentor, for his suggestions given on the progress of this project during presentations  Richard Lin (3M), for his assistance ... Instron micro tester for mechanical testing  Ms Irene Kee (Dept of Experimental Surgery, SGH), for her valuable assistance and support during animal surgeries and taking care for them after implantations ... current treatments for bone defects For surgical procedures involving bone grafts, patients usually suffer from trauma-related injuries or bone fractures Currently, the gold standard for bone grafts...

Ngày tải lên: 09/10/2015, 11:18

... (stereolithography) format into QS (QuickSlice), slicing of the CAD model into horizontal layers and conversion into SLC format, creation of a deposition path for each layer and conversion into SML format for ... engineering for bone regeneration is the scaffold that serves as a 3D template for initial cell interactions and the formation of boneextracellular matrix to provide structural support to the newly formed ... process He has been an immense driving force behind this project One simply could not wish for a better or friendlier supervisor  Dr Bina Rai, mentor, for graciously sharing her knowledge and...

Ngày tải lên: 09/10/2015, 11:18

... cost of life 1.5 Medical Options for Heart Disease 1.5.1 Drugs for Heart Disease The conventional treatment for cardiovascular disease includes specific medication for any underlying causes Various ... for cardiac tissue replacement, further confirming its huge promise for cardiovascular tissue engineering application This thesis reports on a novel hybrid nanofibrous PCL membrane scaffold for ... absorbable biomaterials, to guide the formation of new cardiovascular tissues Initial targets of the research program will include heart valves, vascular grafts, and cardiovascular patches Successful development...

Ngày tải lên: 26/11/2015, 22:40

... surfaces for layered tissue engineering Subsequently, perivascular cells derived from umbilical cord (UCPVC) were found suitable for reconstruction of the perivascular compartment for vascular ... system to be studied 1.2 Vascular Tissue Engineering Vascular tissue engineering presents an attractive model for LBL tissue engineering for several reasons Firstly, vascular tissue is naturally ... 11 1.1.3.1 1.2 Microthin films for use in layer-by-layer tissue engineering 12 Vascular Tissue Engineering .13 1.2.1 Clinical Need For Vascular Tissue Engineering 14 1.2.2...

Ngày tải lên: 14/09/2015, 08:49

... induced endothelial cells to form more interconnected CLS than untreated controls, in a similar manner to VEGF, confirming that it was able to accelerate formation of vascular analogues by endothelial ... scaffolds This demonstrated a proof of concept that PHi could improve neovascularization for tissue engineering applications by pre-vascularization of three dimensional scaffolds viii LIST OF FIGURES ... vessels Tris buffered saline Tris buffered saline with tween-20 Transforming growth factor- β Vascular endothelial growth factor Vascular endothelial growth factor receptor von Willebrand Factor...

Ngày tải lên: 12/09/2015, 08:18

... Chapter Isolation and Characterisation Of Vascular Progenitors Figure 4-3: Primary culture of haemopoietic tissue in EGM Following culture ... cells, and failed to express endothelial phenotype 122 Chapter Isolation and Characterisation Of Vascular Progenitors (a) (b) (c) (d) (e) (f) Figure 4-4: CFU assay Adherent colonies appeared after...

Ngày tải lên: 14/09/2015, 08:49

... Isolation and Characterisation Of Vascular Progenitors 4.3 Discussion 4.3.1 Summary of Results In this chapter, I detailed my efforts to identify good cellular sources for vascular tissue engineering ... Colony forming assays of expanded FL133+ cells generated CFU-E (Colony Forming Unit Erythrocyte, 1.33/103 cells), BFU-E (Burst Forming Unit Erythrocyte, 3.00/103 cells), CFU-M (Colony Forming ... Colony forming assays of expanded FL133+ cells generated haemopoietic colonies including (a) CFU-E (Colony Forming Unit Erythrocyte) (b) BFU-E (Burst Forming Unit Erythrocyte) (c) CFU-M (Colony Forming...

Ngày tải lên: 14/09/2015, 08:49

... improve biocompatibility Vascular progenitors from perinatal sources were also examined for possible use in vascular tissue engineering, together with PCL films, used to generate vascular cell-sheet ... studied the adhesion of perivascular cells to modified PCL film surfaces for the generation of the perivascular compartment I then proceeded to engineer a surface for the reconstitution of the ... must perform the homeostatic regulation of blood clot formation Next, vascular grafts typically fail in the mid-term because of re-occlusion arising from neointimal hyperplasia Existing vascular...

Ngày tải lên: 14/09/2015, 08:49

... by fibroblast form thin fibrils with time But the newly-formed collagen fibrils form only loose irregular network which is not organized (Jozsa et al 1997) This process could last for weeks resulted ... knitted scaffold for in vitro and in vivo tissue formation 1.3.3 Bioreactor System In vivo, tissue formation is directed by multiple factors including: (1) structural template for cell attachment ... cell function for the purpose of ligament/tendon reconstruction   ix List of Tables Table 1.1 Biological grafts for ligament reconstruction surgery 14 Table 1.2 Synthetic grafts for ligament...

Ngày tải lên: 14/09/2015, 14:05

... regeneration site, and a facility for promoting capillary formation, in other words, neovascularization When a permissive environment optimal for cells to regenerate tissues is formed expectedly by these ... attachment, the barrier for cells to retain the site for tissue regeneration, the guide for cells to create a tissue giving the contour of the regenerated tissue, the carrier for the sustained release ... provide an attachment site for the cells responsible to the tissue regeneration Similar to embryonic development, multiple cells should assemble to a specified form for tissue formation To this end,...

Ngày tải lên: 25/10/2013, 08:20

... filament proteins, for example, cytokeratins for epithelial cells, vimentin for mesodermal cells such as fibroblasts, endothelium, and myoblasts, desmin for myocytes, neurofilament protein for neuronal ... Culture of Cells for Tissue Engineering is a new volume in the John Wiley series Culture of Specialized Cells, with focus on procedures for obtaining, manipulating, and using cell sources for tissue ... discussions with our colleagues We made every effort to provide a user-friendly reference for sourcing, characterization, and use of cells for tissue engineering, for researchers with a variety of backgrounds...

Ngày tải lên: 22/03/2014, 15:20

... used as a substrate for PAK Phosphorylated proteins were electrophoresed on 12% SDS/PAGE gels for detecting phosphorylated MBP Cell motility assays Motility assays were performed in triplicate ... necessary for LPA-induced Rac activation in B103 neuroblastoma cells [34] Therefore, the possible involvement of Fig LPA stimulates PAK1 in A2058 cells The cells were serumstarved for 12 h and ... A2058 cells were serum-deprived and treated with or without 10 lM LY294002 (LY) for h before incubating with LPA (2 lM) for Cellular extracts were incubated with GST-PAK-PBD beads The bound proteins...

Ngày tải lên: 30/03/2014, 13:20

... closed systems for autologous adipose cell therapy, such as Cytori's Celution™ system [16] and Tissue Genesis' TGI 1000™ platform [17], which are presently entering clinical trials Unfortunately, ... precursors in vascular repair and lesion formation J Cell Mol Med 2005, 9:557-568 Miranville A, Heeschen C, Sengenes C, Curat CA, Busse R, Bouloumie A: Improvement of postnatal neovascularization ... adipose tissue contain a population of cells positive for CD34, CD133 and the drug efflux pump ABCG2 [30] These cells had endothelial colony forming ability in vitro, and in vivo could induce angiogenesis...

Ngày tải lên: 18/06/2014, 15:20