fi cation and differentiation of bacterial strains

... specific and sensitive assay for the rapid detection and differentiation of wildtype and vaccine strains of CDV For lack of other vaccine strains except CDV Onderstepoort strain, the primers P5 and ... CDV vaccine strain After the application of the first-round PCR, primers P2 and P4, and P3, P4, P5 and P6 together, were used to amplify the vaccine and wild-type strains, respectively, at different ... vaccine strain, respectively Two bands of 247 and 177 bp were detected simultaneously from the mixed genomic RNA of the CDV wild-type and vaccine strains Amplification was not possible for nonCDV...

Ngày tải lên: 12/08/2014, 04:20

... the differentiation of the naive T-cell to Th1, Th2, Th17 or Treg cells may exist in fish In this investigation, we have identified, for the first time, t-bet and stat6 in zebrafish and, for the first ... sequenced within bony fish, in the Ginbuna crucian carp [27], and STAT6 in mandarin fish [28], whereas Foxp3 has been characterized for the first time in fish The availability of sequenced fish genomes has ... organizations of zebrafish t-bet, stat6 and foxp3 were also determined and found to be very similar to their human homologues Both the human and zebrafish T-bet and Foxp3 genes contained the same number of...

Ngày tải lên: 16/02/2014, 09:20

... underground injection and will be used for a number of purposes, such as tracking the location of the plume of injected CO2, ensuring that injection and abandoned wells are not leaking, and verifying ... technologies and approaches to the point of pre-commercial application The program’s core R&D agenda focuses on increased understanding of CO2 GS, MVA technology and cost, and regulations and permitting ... the presence and adequacy of injection and confining zones by presenting information on geologic formations; map of the injection well and AoR3; and maps, cross sections and a list of penetrations...

Ngày tải lên: 18/02/2014, 01:20

... report the identi cation of SmSmad1B cDNA and present its gene structure along with the expression profiles, immunolocalization, and protein interaction properties Results Identi cation of SmSmad1B ... the filter-lift assay, due to the quantifiable nature of the liquid assays Also, the magnitude of the decrease in interaction between SmSmad1 and SmSmad4 in the presence of the receptors, specifically ... properties of SmSmad1 and SmSmad1B as described above However, in the presence of SmSmad4 and the active mutant form of TbRI, both SmSmad2 and SmSmad4 interacted with GCN5, indicating the formation of...

Ngày tải lên: 18/02/2014, 16:20

... measured One unit is defined as the amount of enzyme that forms lmol ornithineÆmin)1 Puri cation of GAT from wild watermelon leaves All puri cation steps were carried out at 0–4 °C, and column chromatographies ... activity and 24-fold puri cation were achieved Heat treatment was followed by six chromatography steps comprising hydrophobic interaction, anion and cation exchange, gel filtration, and hydroxyapatite ... Acetylornithinase of Escherichia coli: partial puri cation and some properties J Biol Chem 218, 97–106 12 Leisinger T & Haas D (1975) N-acetylglutamate synthase of Escherichia coli regulation of synthesis and...

Ngày tải lên: 20/02/2014, 03:20

... units) 20 2000 Results Puri cation of lectin from the hemolymph of P jacquemontii A column profile depicting puri cation of lectin from the hemolymph of P jacquemontii by affinity chromatography on ... revealed that puri cation of lectin from the hemolymph of crustaceans was most successful by affinity chromatography as it gave a higher fold of puri cation and percentage of recovery [20,24,27,35–39] ... Clarified serum (20 mL) was applied and on elution with EDTA yielded 1.0 mg of pure lectin The specific activity of purified lectin increased about 2000-fold from 196 (crude hemolymph) to 409 600 of...

Ngày tải lên: 21/02/2014, 00:20

... the presence of lM okadaic acid, nor did mM EDTA in the absence of mM magnesium chloride inhibit the enzyme Puri cation of the recombinant phosphohistidine phosphatase Puri cation of phosphohistidine ... released orthophosphate was measured by a modi cation of the method of Martin and Doty [21], using toluene instead of benzene To keep background hydrolysis of the acid-labile phosphoamidate as low ... cascade Pharmacol Ther 67, 323–350 Wei, Y.F & Matthews, H.R (1991) Identi cation of phosphohistidine in proteins and puri cation of protein-histidine kinases Methods Enzymol 200, 388–414 Fothergill-Gilmore,...

Ngày tải lên: 21/02/2014, 01:21

... required for growth and potassium tellurite inhibited growth Thus, strain 10d was identified as a species of Bordetella Puri cation and properties of the purified enzyme Fig Growth of strain 10d on ... This is the first report of the puri cation of a 4-amino3-hydroxybenzoate-fission enzyme and its characterization in terms of molecular mass, subunit structure, reaction mechanism and catalytic ... In Classi cation and Identi cation of Microorganism (Hasegawa, T., ed.), pp 99–160 Japan scientific societies press, Tokyo 16 Johnson, R & Sneath, P.H.A (1973) Taxonomy of Bordetella and related...

Ngày tải lên: 21/02/2014, 01:21

... Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipV Cloning of sipV Cloning of sipV Cloning of sipV ... Cloning of sipV Cloning of sipV Cloning of sipV Construction of pOpacVh Construction of pOpacVh Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW ... Construction of pOpacWh Construction of pOpacWh Construction of pOpacSh Construction of pOpacSh Construction of pOpacTh Construction of pOpacTh Construction of pOpacBh Construction of pOpacBh Construction...

Ngày tải lên: 21/02/2014, 03:20

... maximum of the a band at 557 nm absorption maxima at 420 nm (c band), 530 nm (b band) and 557 nm (a band) are characteristic of cytochrome b Heme was extracted from the protein with acidic acetone and ... behavior is typical of integral membrane proteins The polypeptide with an apparent molecular mass of 53 kDa appears as a double band in unboiled samples (lanes A1 and B1) Identi cation of the genes ... by the heterodisulfide of coenzyme M and coenzyme B, or by the homodisulfides of these two coenzymes Also potassium trithionate and sodium tetrathionate, which have been identified in dissimilatory...

Ngày tải lên: 21/02/2014, 03:20

... the following observations support the classi cation of UP12 as a member of the UspA family Sub-cloning, puri cation, and characterization of UP12 and its interaction with GroEL In order to investigate ... (Eur J Biochem 269) Fig Puri cation of His6–UP12, characterization of the anti-UP12 Ig and coimmunoprecipitation of UP12 with GroEL from cell extracts (A) Puri cation of UP12 His6–UP12 as a hybrid ... Effect of addition of mM DNP on cell growth (h) and expression of UP12 (j) (B) Monitoring of cell growth (s) and amount of UP12 (d) before and after addition of 0.1 mM CCCP (C) Effect of temperature...

Ngày tải lên: 22/02/2014, 07:20

... phylogenetic analysis of fish hatching enzyme genes, hatching enzyme originally consisted of a single type of enzyme, and HCE and LCE were produced by duplication and diversi cation of the gene [16] ... enzymes and substrates of both species: zebrafish and medaka Hatching enzyme of zebrafish Results Expression of zebrafish hatching enzyme ZHE1 and ZHE2 genes It has been reported that two cDNAs, ZHE1 and ... with those of ZHE1 and MHCE However, the ratios of the specific activities of MLCE toward the three substrates were similar to that of ZHE1 and MHCE In summary, the substrate specificity of MHCE toward...

Ngày tải lên: 07/03/2014, 04:20

... regulation of a number of genes [7–10] Study of SmRXR partners will add to our knowledge of nuclear receptor gene regulation in schistosomes and to an understanding of the evolution of RXR’s function ... development by iQ5 application (cDNA is diluted to ·1-, ·10-, ·100- and ·1000-fold; see protocol of Bio-Rad iQ5 application) Normalized gene expression [23] of SmNR1 was calculated and standardized to ... Isolation and characterization of Schistosoma mansoni constitutive androstane receptor Mol Biochem Parasitol 148, 31–43 14 Lu C, Wu W, Niles EG & LoVerde PT (2006) Identi cation and characterization of...

Ngày tải lên: 07/03/2014, 11:20

... two isoforms of caricain and five isoforms of chymopapain from C papaya From the genus Vasconcellea, only the latex of V cundinamarcensis has previously been studied in detail Isolation and preliminary ... the five chymopapain isoforms of C papaya with VXH-C, VS-B, CC-III and CC23 These results confirm the hypothesis of Walraevens et al [17] that CC-III is a chymopapain homologue and predict VXH-C and ... Fractionation and puri cation of the enzymes stored in the latex of Carica papaya J Chrom B 790, 229–238 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage...

Ngày tải lên: 07/03/2014, 11:20

... on HisTrap HP and gel filtration on HiLoad Superdex 75 obtaining 30 mg and 18 mg, respectively The SDS ⁄ PAGE of the final preparation revealed a single band with a molecular mass of 25 ± kDa (Fig ... cells and purified from the soluble fraction of bacterial cells as described in Experimental procedures The yield of C49S was the same of that obtained for the wild-type protein The activity of C49S ... absence of putative catalases and the presence of four putative Bcps proteins: Bcp1, Bcp2, Bcp3, Bcp4 whose roles should be clarified in detail and could play a key role in the detoxi cation processes...

Ngày tải lên: 07/03/2014, 12:20

... (cytoplasmic marker) and succinate dehydrogenase (cytoplasmic membrane marker) Puri cation of tetrathionate hydrolase All puri cation procedures were performed at °C Washed cells (6 g of wet weight) ... single spot and the pI of the tetrathionate hydrolase was 9.8 The molecular mass of the purified tetrathionate hydrolase was determined by SDS electrophoresis and specified by MALDI-TOF MS The enzyme ... pentathionate puri cation procedure The present study is the first to demonstrate the puri cation of tetrathionate hydrolase without ammonium sulfate in the buffers Dialysis of the crude enzyme...

Ngày tải lên: 07/03/2014, 14:20

... variations of the argument of the argmax in equation 1: (1) instead of considering Score(Sh−1 ), consider only the last segment contained in Sh−1 ; and (2) instead of using the product of the scores of ... Research, Development, and Engineering Command (RDECOM) Statements and opinions expressed not necessarily reflect the position or the policy of the United States Government, and no of cial endorsement ... for the five most common dialogue act labels in our dataset The average number of words in utterances with only a single dialogue act is 7.5 (with a maximum of 34, and minimum of 1), and the average...

Ngày tải lên: 07/03/2014, 22:20

... revealed that a large part of the 5¢-UTR and the complete N-terminal Ó FEBS 2003 specific part of HPR130 reside in one exon of 2435 bp (exon 2a) Comparison of the XN73 and XPR130 sequences with ... prophase and metaphase oocytes) A reverse transcriptase reaction was performed using primers containing the translation stop of the different isoforms to obtain specific cDNA After puri cation of this ... with cdc6 and modulates DNA replication in human cells Mol Cell Biol 20, 1021–1029 Ó FEBS 2003 28 Waelkens, E., Goris, J & Merlevede, W (1987) Puri cation and properties of the polycation-stimulated...

Ngày tải lên: 08/03/2014, 08:20

... (Cs+ ions of energy of kV) Extraction and puri cation of F 4¢-OMT All the puri cation steps were carried out at °C temperature The enzyme was concentrated at various steps of puri cation using ... Sweden) Results Fig Puri cation of the flavonoid 4¢-OMT through gel filtration Enzymatic activity is expressed as nkatÆmg protein)1 Puri cation of F 4¢-OMT The whole sequence of chromatographic steps ... in the course of time: a storage period of weeks at )20 °C caused a  50% loss of activity The different phases of F 4¢-OMT puri cation are presented in Table The enzyme was purified 1399-fold,...

Ngày tải lên: 08/03/2014, 08:20

... DEAE-Toyopearl puri cation; lane 4, protein after Butyl-Toyopearl puri cation; lane 5, protein after Sephacryl HR S-200 puri cation; lane 6, protein after FPLC-MonoQ puri cation step (B) Western ... Puri cation and properties of D-aminoacylase of Streptomyces olivaceus Agric Bio Chem 42, 107–113 Ó FEBS 2002 19 Tsai, Y.C., Tseng, C.P., Hsiao, K.M & Chen, L.Y (1988) Production and puri cation ... ampli cation was carried out as follows: 94 °C for followed by 30 cycles of 30 s at 94 °C, 30 s at 62 °C and 30 s at 72 °C and then a further extension at 72 °C The PCR mix before amplification...

Ngày tải lên: 08/03/2014, 16:20