fi cation and detection of hcv rna
Báo cáo khoa học: Identification of a preferred substrate peptide for transglutaminase 3 and detection of in situ activity in skin and hair follicles pdf
... concentration of the purified protein was determined by quanti cation of the intensity for the separated bands in SDS ⁄ PAGE analysis using imaging software (multigauge software; Fujifilm, Tokyo, ... activity in both human and mouse skin [16,19] These studies have provided new insight into the substrate specificity of Tgases and have expanded the range of application of the enzyme reaction ... Avidin affinity puri cation resulted in the selection of phage particles that covalently bound bio-Cd The phage particles were amplified and subjected to four additional enzymatic reactions and panning...
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Development of gold nanoparticle DNA nanostructure assembly for detection of DNA, RNA and protein biomarkers
... Design of miRNA sequences and detection concept 55 4.2.3 Fabrication of gold nanoparticle probes 56 iii 4.2.4 4.2.5 4.3 DLS Detection 57 Variation of probe types and ... controlled Scheme 2.1 Formation of mature miRNA from pri- and pre-miRNA via the actions of Drosha and Dicer, and the RISC complex that regulates mRNA levels [12] MiRNA expression fingerprint was ... screening of G-quadruplex stabilizing ligands and colorimetric detection of proteins [150, 151] As already mentioned in the earlier, the association of miRNA with mRNA led to the activation of a regulatory...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
... differentiation of the naive T-cell to Th1, Th2, Th17 or Treg cells may exist in fish In this investigation, we have identified, for the first time, t-bet and stat6 in zebrafish and, for the first time in any fish ... sequenced within bony fish, in the Ginbuna crucian carp [27], and STAT6 in mandarin fish [28], whereas Foxp3 has been characterized for the first time in fish The availability of sequenced fish genomes has ... and will remain cytosolic Also found in the 3¢-UTR of zebrafish stat6 and foxp3 were numerous copies of an mRNA instability motif (attta) which plays a role in mRNA degradation [33], typical of...
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Tài liệu BEST PRACTICES for: Monitoring, Verifi cation, and Accounting of CO2 Stored in Deep Geologic Formations doc
... underground injection and will be used for a number of purposes, such as tracking the location of the plume of injected CO2, ensuring that injection and abandoned wells are not leaking, and verifying ... technologies and approaches to the point of pre-commercial application The program’s core R&D agenda focuses on increased understanding of CO2 GS, MVA technology and cost, and regulations and permitting ... deformation) and assess the level of induced seismicity in regard to safety of existing surface infrastructure and as an alternative means of mapping the spread of CO2 within the reservoir An array of...
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Tài liệu Báo cáo khoa học: Identification and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf
... report the identi cation of SmSmad1B cDNA and present its gene structure along with the expression profiles, immunolocalization, and protein interaction properties Results Identi cation of SmSmad1B ... the filter-lift assay, due to the quantifiable nature of the liquid assays Also, the magnitude of the decrease in interaction between SmSmad1 and SmSmad4 in the presence of the receptors, specifically ... properties of SmSmad1 and SmSmad1B as described above However, in the presence of SmSmad4 and the active mutant form of TbRI, both SmSmad2 and SmSmad4 interacted with GCN5, indicating the formation of...
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Tài liệu Báo cáo khoa học: Purification and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon doc
... measured One unit is defined as the amount of enzyme that forms lmol ornithineÆmin)1 Puri cation of GAT from wild watermelon leaves All puri cation steps were carried out at 0–4 °C, and column chromatographies ... activity and 24-fold puri cation were achieved Heat treatment was followed by six chromatography steps comprising hydrophobic interaction, anion and cation exchange, gel filtration, and hydroxyapatite ... Acetylornithinase of Escherichia coli: partial puri cation and some properties J Biol Chem 218, 97–106 12 Leisinger T & Haas D (1975) N-acetylglutamate synthase of Escherichia coli regulation of synthesis and...
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Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf
... units) 20 2000 Results Puri cation of lectin from the hemolymph of P jacquemontii A column profile depicting puri cation of lectin from the hemolymph of P jacquemontii by affinity chromatography on ... revealed that puri cation of lectin from the hemolymph of crustaceans was most successful by affinity chromatography as it gave a higher fold of puri cation and percentage of recovery [20,24,27,35–39] ... Clarified serum (20 mL) was applied and on elution with EDTA yielded 1.0 mg of pure lectin The specific activity of purified lectin increased about 2000-fold from 196 (crude hemolymph) to 409 600 of...
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Tài liệu Báo cáo Y học: Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf
... the presence of lM okadaic acid, nor did mM EDTA in the absence of mM magnesium chloride inhibit the enzyme Puri cation of the recombinant phosphohistidine phosphatase Puri cation of phosphohistidine ... centrifugation of the supernatant at 14 500 g for The latter supernatant was then centrifuged at 45 500 g for h Puri cation of phosphohistidine phosphatase from porcine liver cytosol A mixture of 320 ... lgÆmL)1 pepstatin A and mM EDTA and passed four times through a French Press The cell lysate was centrifuged at 17 000 g for 10 and the resulting supernatant was used for puri cation of the recombinant...
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Tài liệu Báo cáo Y học: A novel meta-cleavage dioxygenase that cleaves a carboxyl-groupsubstituted 2-aminophenol Purification and characterization of 4-amino-3-hydroxybenzoate 2,3-dioxygenase from Bordetella sp. strain 10d doc
... required for growth and potassium tellurite inhibited growth Thus, strain 10d was identified as a species of Bordetella Puri cation and properties of the purified enzyme Fig Growth of strain 10d on ... This is the first report of the puri cation of a 4-amino3-hydroxybenzoate-fission enzyme and its characterization in terms of molecular mass, subunit structure, reaction mechanism and catalytic ... In Classi cation and Identi cation of Microorganism (Hasegawa, T., ed.), pp 99–160 Japan scientific societies press, Tokyo 16 Johnson, R & Sneath, P.H.A (1973) Taxonomy of Bordetella and related...
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Tài liệu Báo cáo khoa học: Identi®cation and properties of type I-signal peptidases of Bacillus amyloliquefaciens doc
... Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipV Cloning of sipV Cloning of sipV Cloning of sipV ... Cloning of sipV Cloning of sipV Cloning of sipV Construction of pOpacVh Construction of pOpacVh Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW ... Construction of pOpacWh Construction of pOpacWh Construction of pOpacSh Construction of pOpacSh Construction of pOpacTh Construction of pOpacTh Construction of pOpacBh Construction of pOpacBh Construction...
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Tài liệu Báo cáo khoa học: Purification and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulfide reductase from methanogenic archaea pdf
... maximum of the a band at 557 nm absorption maxima at 420 nm (c band), 530 nm (b band) and 557 nm (a band) are characteristic of cytochrome b Heme was extracted from the protein with acidic acetone and ... behavior is typical of integral membrane proteins The polypeptide with an apparent molecular mass of 53 kDa appears as a double band in unboiled samples (lanes A1 and B1) Identi cation of the genes ... by the heterodisulfide of coenzyme M and coenzyme B, or by the homodisulfides of these two coenzymes Also potassium trithionate and sodium tetrathionate, which have been identified in dissimilatory...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... (A) Effect of addition of mM DNP on cell growth (h) and expression of UP12 (j) (B) Monitoring of cell growth (s) and amount of UP12 (d) before and after addition of 0.1 mM CCCP (C) Effect of temperature ... (Eur J Biochem 269) Fig Puri cation of His6–UP12, characterization of the anti-UP12 Ig and coimmunoprecipitation of UP12 with GroEL from cell extracts (A) Puri cation of UP12 His6–UP12 as a hybrid ... the following observations support the classi cation of UP12 as a member of the UspA family Sub-cloning, puri cation, and characterization of UP12 and its interaction with GroEL In order to investigate...
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Báo cáo khoa học: Purification and characterization of zebrafish hatching enzyme – an evolutionary aspect of the mechanism of egg envelope digestion pot
... consisted of a single type of enzyme, and HCE and LCE were produced by duplication and diversi cation of the gene [16] As comparing the egg envelope digestion mechanism between zebrafish and medaka, ... enzymes and substrates of both species: zebrafish and medaka Hatching enzyme of zebrafish Results Expression of zebrafish hatching enzyme ZHE1 and ZHE2 genes It has been reported that two cDNAs, ZHE1 and ... with those of ZHE1 and MHCE However, the ratios of the specific activities of MLCE toward the three substrates were similar to that of ZHE1 and MHCE In summary, the substrate specificity of MHCE toward...
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Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot
... regulation of a number of genes [7–10] Study of SmRXR partners will add to our knowledge of nuclear receptor gene regulation in schistosomes and to an understanding of the evolution of RXR’s function ... rapid ampli cation of cDNA ends (RACE) using SMARTTM RACE cDNA Ampli cation Kit (BD Biosciences Clontech, Mountain View, CA 94043, USA) The sequence was confirmed as belonging to a single mRNA species ... Isolation and characterization of Schistosoma mansoni constitutive androstane receptor Mol Biochem Parasitol 148, 31–43 14 Lu C, Wu W, Niles EG & LoVerde PT (2006) Identi cation and characterization of...
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Báo cáo khoa học: Purification and characterization of the cysteine proteinases in the latex of Vasconcellea spp. ppt
... two isoforms of caricain and five isoforms of chymopapain from C papaya From the genus Vasconcellea, only the latex of V cundinamarcensis has previously been studied in detail Isolation and preliminary ... the five chymopapain isoforms of C papaya with VXH-C, VS-B, CC-III and CC23 These results confirm the hypothesis of Walraevens et al [17] that CC-III is a chymopapain homologue and predict VXH-C and ... Fractionation and puri cation of the enzymes stored in the latex of Carica papaya J Chrom B 790, 229–238 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage...
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Báo cáo khoa học: Identification and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress pdf
... transcriptional start site, and the BRE motif, targets for the general transcription factors TBP and TFB, respectively [17] bcp2 mRNA B 16S rRNA Puri cation and characterization of recombinant Bcp2 (rBcp2) ... purified to homogeneity in a two-stage process using affinity chromatography on HisTrap HP and gel filtration on HiLoad Superdex 75 obtaining 30 mg and 18 mg, respectively The SDS ⁄ PAGE of the final ... mass suggested the removal of the N-terminal methionine To assess the quaternary structure of the enzyme, analytical gel filtration on PC 75 and Biosep-SEC-4000 of purified rBcp2 were performed The...
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Báo cáo khóa học: Localization, purification and properties of a tetrathionate hydrolase from Acidithiobacillus caldus pot
... (cytoplasmic marker) and succinate dehydrogenase (cytoplasmic membrane marker) Puri cation of tetrathionate hydrolase All puri cation procedures were performed at °C Washed cells (6 g of wet weight) ... single spot and the pI of the tetrathionate hydrolase was 9.8 The molecular mass of the purified tetrathionate hydrolase was determined by SDS electrophoresis and specified by MALDI-TOF MS The enzyme ... pentathionate puri cation procedure The present study is the first to demonstrate the puri cation of tetrathionate hydrolase without ammonium sulfate in the buffers Dialysis of the crude enzyme...
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Báo cáo khoa học: "Joint Identification and Segmentation of Domain-Specific Dialogue Acts for Conversational Dialogue Systems" doc
... variations of the argument of the argmax in equation 1: (1) instead of considering Score(Sh−1 ), consider only the last segment contained in Sh−1 ; and (2) instead of using the product of the scores of ... Research, Development, and Engineering Command (RDECOM) Statements and opinions expressed not necessarily reflect the position or the policy of the United States Government, and no of cial endorsement ... for the five most common dialogue act labels in our dataset The average number of words in utterances with only a single dialogue act is 7.5 (with a maximum of 34, and minimum of 1), and the average...
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Báo cáo khoa học: Identification and characterization of B¢¢-subunits of protein phosphatase 2 A in Xenopus laevis oocytes and adult tissues Evidence for an independent N-terminal splice variant of PR130 and an extended human PR48 protein pot
... Total RNA from prophase oocytes and adult tissues was prepared as described [40] RNA was further purified using a mini Qiagen RNA puri cation kit (Westburg) cDNA was prepared from 1.5 lg of total RNA ... performed on about 1.5 lg of total RNA of prophase X laevis oocytes with U of AMVreverse transcriptase (5¢/3¢RACE kit, Roche) For the first strand cDNA synthesis, to amplify 5¢ ends of XN73, the primer ... revealed that a large part of the 5¢-UTR and the complete N-terminal Ó FEBS 2003 specific part of HPR130 reside in one exon of 2435 bp (exon 2a) Comparison of the XN73 and XPR130 sequences with...
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Báo cáo khoa học: Purification and properties of a new S-adenosyl-Lmethionine:flavonoid 4¢-O-methyltransferase from carnation (Dianthus caryophyllus L.) pot
... (Cs+ ions of energy of kV) Extraction and puri cation of F 4¢-OMT All the puri cation steps were carried out at °C temperature The enzyme was concentrated at various steps of puri cation using ... Sweden) Results Fig Puri cation of the flavonoid 4¢-OMT through gel filtration Enzymatic activity is expressed as nkatÆmg protein)1 Puri cation of F 4¢-OMT The whole sequence of chromatographic steps ... in the course of time: a storage period of weeks at )20 °C caused a 50% loss of activity The different phases of F 4¢-OMT puri cation are presented in Table The enzyme was purified 1399-fold,...
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