μpiv and velocity profiles in vitro and in vivo

... TURBULENT VELOCITY PROFILES IN OPEN-CHANNEL FLOWS TIN MIN THANT (B.Eng.(Civil),YTU) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF ENGINEERING DEPARTMENT OF CIVIL ENGINEERING NATIONAL UNIVERSITY OF SINGAPORE ... flow transporting neutrally buoyant particles of a single size They reported a decrease in κ, and an increase in turbulence intensity with increasing particle concentration Montes and Ippen (1973) ... power-law velocity profiles are in excellent agreement with measured concentration profiles In the analysis that follows, the effects of moving sediment on the velocity profiles are incorporated into...

Ngày tải lên: 05/10/2015, 19:04

... (as shown in the following figure), and install CMAK Install Phone Book Administrator Open Windows Explorer, and browse the installation disc for Windows Server 2003, Standard Edition Install PBA ... example.com domain to a native Windows Server 2003 domain Install and configure DHCP Install Click DHCP as a subcomponent of the Networking Services component Start, point to Administrative Tools, and click ... wizard, and configure the modem Configure Routing and Remote Access Click Start, point to Administrative Tools, and click Routing and Remote Access In the console tree, right-click VPN1, and click...

Ngày tải lên: 27/01/2014, 15:20

... recombinant cytokines was used to establish standard curves and to maximize the sensitivity and dynamic range of the assay Cytokine levels were determined using a Luminex IS 100 (Luminex, Austin, ... factors and underlying diseases Additionally, data regarding cytokine profiling in the elderly have been limited to a small subset of cytokines In this study, we compared the profiles of 22 cytokines, ... participated in the study design, result interpretation and in the writing HOK and HSK performed the analysis of the data and drafted the manuscript JCY and ECS participated in the design of the study and...

Ngày tải lên: 14/02/2014, 06:20

... ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) and 1% nonessential amino acids (Gibco Invitrogen) Murine hepatoma-1 ... all-trans-retinoic acid, the findings clearly demonstrate that S-4o9cDH-RA has the capacity to bind and activate nuclear retinoid receptors and regulate gene transcription both in vitro and in vivo Retinoids ... grown in low-glucose DMEM (Gibco Invitrogen) supplemented with 10% (v ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) and...

Ngày tải lên: 18/02/2014, 08:20

... USA) and transformed into E coli for protein induction Extracts prepared from cells induced for h were shown to contain a six-histidine C-terminal-tagged OsRPA14 fusion protein The OsRPA14 protein ... tag were obtained by in vitro translation in wheatgerm extract (Invitrotec) according to the manufacturer’s procedure OsRPA70a (or OsRPA70b) and OsRPA32, or OsRPA32 and OsRPA14 were incubated with ... soluble in the presence of m urea Soluble fractions were loaded on to columns containing 10 mL His-Bind resin (Novagen) The columns were washed with 50 mL binding buffer containing m urea and then...

Ngày tải lên: 07/03/2014, 21:20

... shown) In conclusion, we have shown using both in vitro and in vivo methods, that PSI-G adopts a topology in the thylakoid membrane with the loop facing the stroma and its N- and C-termini facing ... promoter and terminator, and into vectors for in vitro transcription and translation An Arabidopsis line (psag-1.4 [5], termed DG in this report), lacking PSI-G due to a transposon footprint in exon ... after 0, 30 and 60 digestion with trypsin, probed with antibodies directed against PSI-G, PSI-K (intrinsic membrane protein, stromal loop), PSI-O (intrinsic membrane protein, luminal loop) and PSI-D...

Ngày tải lên: 07/03/2014, 21:20

... CPS and O-chain LPS are composed of linear trisaccharide repeating units containing 3-linked 2-acetamido-2-deoxy-D-quinovose, 4-linked 3-[(N-acetyl-L-alanyl) amido]-3-deoxy-D-quinovose and 3-linked ... Interresidue NOEs were observed between H-1a and H-3c and between H-1b and H-3a, suggesting a linear structure a-c-b (Fig 2E,F) In addition, interresidue NOEs between H-1c and H-4b (Fig 2G) indicated ... and O-chain polysaccharide of A salmonicida strain 80204-1 the previously identified O-chain containing L-rhamnose, 2-amino-2-deoxy-D-mannose and D-glucose [11], confirming that the novel CPS and...

Ngày tải lên: 23/03/2014, 13:20

... the protein The self-cleavage of LexA protein is important in the SOS response in bacteria [21–23] In vitro self-cleavage was also observed in all investigated bacterial SPase I including enzymes ... results intrigued us to investigate the cleavage of S pneumoniae SPase I in vivo As shown in Fig 6C, Western blot analysis demostrated that the self-cleavage of SPase I is indeed occuring in vivo in ... product in exponential growth phase In general, the bacterial cells maintained the active SPase I in the highest level in Fig Cell growth of S pneumoniae and Western blot analysis of in vivo self-cleavage...

Ngày tải lên: 23/03/2014, 21:21

... nonspecific binding by protein–protein interactions, cells were incubated with 100 lg ligand-free carrier protein (BSA) per mL for 30 at °C before incubation with the biotinylated neoglycoprotein in Dulbecco’s ... assay, saturable and carbohydrate-dependent binding was measured, and the resulting Scatchard plots gave straight lines indicating the presence of a single class of binding sites in each case (not ... neoglycoproteins, we determined their binding to cells and to tissue sections as well as their biodistribution in mice after injection of radioiodinated material Binding to cells and organs In these...

Ngày tải lên: 30/03/2014, 13:20

... recombinant cytokines was used to establish standard curves and to maximize the sensitivity and dynamic range of the assay Cytokine levels were determined using a Luminex IS 100 (Luminex, Austin, ... factors and underlying diseases Additionally, data regarding cytokine profiling in the elderly have been limited to a small subset of cytokines In this study, we compared the profiles of 22 cytokines, ... participated in the study design, result interpretation and in the writing HOK and HSK performed the analysis of the data and drafted the manuscript JCY and ECS participated in the design of the study and...

Ngày tải lên: 18/06/2014, 22:20

... evaluated in cell culture and in intranasally infected mice The deletion of F2L had a minimal impact on viral replication in vitro and did not appear to significantly reduce the virulence in vivo ... repeatable increase in the efficacy of N-methanocarbathymidine (N-MCT) (Table 2) This compound is a carbocyclic thymidine analog that inhibits the replication of VV both in vitro and in vivo [12,13], and ... differences in replication in mice reflect real differences in the biology of these viruses, or are related to differences in the animal models including route of infection In HSV, direct intracranial inoculation...

Ngày tải lên: 20/06/2014, 01:20

... reports describing the in vitro inhibitory effect of compounds such as ribavirin, mycophenolic acid, imino sugars, inhibitors of serine protease, RNA interference and non-steroidal anti-inflammatory ... lanes and – in vitro translation products of RNA obtained form JEV infected PS cells (untreated) at and 10 hours post infection respectively Lanes and – in vitro translation product of RNA obtained ... Comparative study of the effects of isatin, an endogenous MAO-inhibitor, and seleginine on bradykinasia and dopamine levels in a rat model Parkinson's disease induced by the Japanese encephalitis...

Ngày tải lên: 20/06/2014, 01:20

... J, Ince N, Ouyang N, Min J, Chen J, Shankar P, Lieberman J: RNA interference targeting Fas protects mice from fulminant hepatitis Nat Med 2003, 9:347-351 Gitlin L, Karelsky S, Andino R: Short interfering ... growing in a monolayer, and having a well-defined tendency for parallel orientation Viral infection causes a marked CPE resulting in total cellular detachment, rounding, and destruction, which ... 36 and 48 h postinfection Samples of supernatant were obtained at designated time points, and the TCID50 were determined by the Reed-Muench formula BHK-21 cells are fibroblastic, growing in a...

Ngày tải lên: 20/06/2014, 01:20

... recombinant cytokines was used to establish standard curves and to maximize the sensitivity and dynamic range of the assay Cytokine levels were determined using a Luminex IS 100 (Luminex, Austin, ... factors and underlying diseases Additionally, data regarding cytokine profiling in the elderly have been limited to a small subset of cytokines In this study, we compared the profiles of 22 cytokines, ... participated in the study design, result interpretation and in the writing HOK and HSK performed the analysis of the data and drafted the manuscript JCY and ECS participated in the design of the study and...

Ngày tải lên: 20/06/2014, 04:20

... in mediating the effects of FSH on inhibin-A, activin-A, oestradiol and progesterone secretion and also important in mediating IGF-I stimulated inhibin-A, activin-A, follistatin, oestradiol and ... previously in the bovine model Increases in Akt and Erk signalling proteins in response to FSH and IGF stimulation suggest a role for Akt and Erk signal transduction pathways in FSH and IGF mediated ... progesterone and androstenedione in bovine theca cells in vitro[ 38] The results from Experiment clearly indicate that treatment of follicles in vivo with inhibiters of the Akt and Erk pathways in the...

Ngày tải lên: 20/06/2014, 07:20

... distribution in these last two studies was similar to what was obtained with the i.v injected 11 1In- labeled panitumumab F(ab’)2 already discussed Discussion The in vivo and in vitro properties of the intact ... radiolabelings TN performed PET imaging and analysis MWB synthesized the chelate and participated in the design of the studies DEM conceived of and coordinated the studies and assisted with the in vivo ... 48-96 h post-injection [13] The objective of this study was to evaluate the in vivo and in vitro properties of panitumumab F(ab’)2 fragment and to assess its utility as a targeting agent in radioimmunodiagnostic...

Ngày tải lên: 21/06/2014, 02:20

... cultured in the cell culture medium containing μg/ mL adriamycin (Sigma) Both cell lines were maintained in RPMI-1640 medium containing 10% FCS, 100 U/ml of penicillin, and 100 μg/ml of streptomycin ... and fixed in 100% methanol for 10 Cell monolayers were blocked in 5% BSA in PBS for 45 and incubated for h at room temperature with P-gp antibodies (Invitrogen, Beijing, China), followed by incubation ... were suspended in 100 μL of culture medium and subcutaneously inoculated into the right flank of mice using a 1.0 mL syringe Intravenous injection of reagents and tumor growth inhibition study...

Ngày tải lên: 21/06/2014, 03:20

... immunomodulating properties in in vivo mouse model MATERIAL AND METHODS Survival of Lactobacillus strains at low pH and in the presence of bile salt Lactobacillus strains isolated from feces of healthy infants ... 2000) Experimental design in vivo Eight-week-old inbred BALB/c mice (n = per group) were used for in vivo experiments Strains selected in in vitro tests were mixed in equal proportions, lyophilized ... Serum and Vaccine Producer Biomed, Cracow, Poland), Salmonella enterica subsp enterica sv Enteritidis (clinical isolate obtained from the Institute of Sea and Tropical Medicine, Gdynia, Poland),...

Ngày tải lên: 07/07/2014, 19:20

... process involving increased arterial inflow and restricted venous outflow, coordinated with corpus cavernosum and penile arterial smooth muscle relaxation Any problem in this mechanism results in ... Central and West Asia and distributed throughout the Mediterranean basin In Turkey, the pistachio is grown mainly in the city of Gaziantep.[4] Pistachio nuts are rich sources of plant proteins, ... reasons predominate in the etiology of ED and it frequently appears along with atherosclerosis It is known that atherosclerotic lesions prevent blood flow into cavernosal tissues resulting in ED Pistachio...

Ngày tải lên: 29/07/2014, 02:21

... provide rapid in vitro screens for analyzing new therapies and discovering means of achieving selective protection or targeted killing In light of the ease of use of these in vitro and in vivo assays, ... TUNEL staining, doublecortin staining, S-100b staining, and the merged image (a-d) Images taken at -4 µm; (e-h) -2 µm; (i-l) µm; (m-p) µm The congruence between the doublecortin+ staining and the ... with cytarabine leads to increased and prolonged cell death, and decreased BrdU incorporation, in the adult mouse CNS Cell death and BrdU incorporation were examined as in Figures and (a) The...

Ngày tải lên: 06/08/2014, 18:21