confocal microscopy of skin in vitro and ex vivo

... analysis and interpretation of data of erythrocyte aggregation of the in vitro experiments GP: acquisition, analysis and interpretation of data of erythrocyte deformability of the in vitro experiments ... Consequently, its increase may be explained by the rise in fibrinogen and/ or globulin concentration and/ or to the modification of the erythrocyte surface properties In our in vitro experiments, it ... chromatography Also involved in drafting the manuscript, revising it critically and in giving final approval of the version to be published LU: acquisition, analysis and interpretation of data of laboratory...

Ngày tải lên: 10/08/2014, 05:21

... corresponding siRNAs (P = 0.002 and 0.0004, respectively) siRNAs of SPARC and Ctgf ameliorated fibrosis in skin and reduced inflammation in lungs induced by bleomycin HE stains of mouse skin tissues ... lung and skin were fixed in 4% formalin and embedded in paraffin Sections of μm were stained either with hematoxylin and eosin (HE) and Masson's trichrome Statistical analysis Results were expressed ... popular bleomycin-induced skin and pulmonary fibrosis in mice Subcutaneous injection and intratracheal instillation of siRNAs were used for tissue-specific treatments of skin and pulmonary fibrosis,...

Ngày tải lên: 12/08/2014, 12:20

... doses in vivo, may have clinical utility In addition, in combination studies using both AZT and WR1065 in human TCBs, we found that addition of WR1065 to a non-saturating dose of AZT resulted in ... three experiments to examine inhibition of HIV-1 replication with the combination of AZT and WR1065 (Table 3) In each experiment, we compared two AZT dose-response curves, one with increasing doses ... prevention of maternal-fetal HIV-1 transmission have been instrumental in prolonging the lives of adults and saving the lives of thousands of children [1-4] However, concern regarding mitochondrial and...

Ngày tải lên: 10/08/2014, 05:21

... For each experiment, the indicated proteins were incubated together in NaCl ⁄ Pi containing 250 mM NaCl at °C for h, and then incubated with Talon beads The amount of protein present in the fraction ... telangiectasia-mutated and Rad3-related) in vitro via ATR interacting protein [4,22,23] Rad17 and Rad9 complexes (Rad17–RFC2–5 and Rad9–Rad1–Hus1) play a crucial role in the activation of the ATR-mediated ... damage and DNA replication stress response pathway RPA is also required to recruit and activate Rad17 complexes for checkpoint signaling in vivo It may function in the sensing of DNA damage [22] In...

Ngày tải lên: 07/03/2014, 21:20

... shown in Fig 4C Interestingly, both tags are recognized more efficiently in the context of the PSI-G loop region than at the C-terminus Topology of PSI-G in vivo In order to examine the topology of ... 386 and 387, or at the extreme C-terminus of PSI-G (Fig 1) These cassettes were both cloned into binary vectors under the control of the 35S promoter and terminator, and into vectors for in vitro ... loops exhibit enough variation to leave room for altered protein–protein interactions, which may explain why the Chlamydomonas loop region is exposed to trypsin in the in vitro assay The in vitro...

Ngày tải lên: 07/03/2014, 21:20

... CPS and O-chain LPS are composed of linear trisaccharide repeating units containing 3-linked 2-acetamido-2-deoxy-D-quinovose, 4-linked 3-[(N-acetyl-L-alanyl) amido]-3-deoxy-D-quinovose and 3-linked ... O-chain containing L-rhamnose, 2-amino-2-deoxy-D-mannose and D-glucose [11], confirming that the novel CPS and O-chain polysaccharide were produced during the 72-h in vivo culture period In addition, ... (GalNAcAcontaining trisaccharide repeating unit) (Fig 4A, inset), based on this ratio about 40% of GalNAcA in both CPS and O-chain polysaccharides was present in the form of a primary amine When...

Ngày tải lên: 23/03/2014, 13:20

... inactivates the protein The self-cleavage of LexA protein is important in the SOS response in bacteria [21–23] In vitro self-cleavage was also observed in all investigated bacterial SPase I including ... pneumoniae SPase I For in vitro self-cleavage of S pneumoniae SPase I in the presence of phospholipid, 20 lL of reaction containing lg of purified SPase I was incubated in 37 °C for h in 20 mM Tris/HCl ... X-100, 10% glycerol, and 50 lg of E coli lipid extract For selfcleavage in the absence of phospholipid, 20 lL of reaction containing lg of purified SPase I was incubated at 37 °C in the same buffer...

Ngày tải lên: 23/03/2014, 21:21

... lines of diverse histogenetic origin The median signal of binding of the fluorescent probe is given as extent of carbohydrate-ligand-dependent binding by subtracting carbohydrate-independent binding ... lectins, quantitation of cell binding comprises the complex display of carbohydrate-binding activities including the occurrence of E-PHA-like human lectins We monitored the ligand properties of ... Mixtures of lactose and (asialo)fetuin were expected to be potent inhibitors Carbohydrate-independent binding of the neoglycoprotein and biotin-independent binding of the second-step reagent was subtracted...

Ngày tải lên: 30/03/2014, 13:20

... structure of a network, including the devices and media that are necessary for successful communications Explain the function of protocols in network communications Explain the advantages of using ... protocols Now – Industry standards Institute of Electrical and Electronics Engineers (IEEE) Examples: 802.3 (Ethernet), 802.11 (WLAN) Internet Engineering Task Force (IETF) Internet standards RFCs ... packet Internetwork Protocol (IP) Assigns the appropriate source and destination addresses, Original source address of host Final destination address of host Used by routers in selecting the...

Ngày tải lên: 01/04/2014, 12:20

... against JEV and highly active against WNV The kinetics of action of SCH 16 in relation to the replicative cycle of JEV in vitro As a first step to understand JEV and SCH 16 interactions, experiments ... reports describing the in vitro inhibitory effect of compounds such as ribavirin, mycophenolic acid, imino sugars, inhibitors of serine protease, RNA interference and non-steroidal anti-inflammatory ... lanes and – in vitro translation products of RNA obtained form JEV infected PS cells (untreated) at and 10 hours post infection respectively Lanes and – in vitro translation product of RNA obtained...

Ngày tải lên: 20/06/2014, 01:20

... could maintain the siRNA target sequences without alterations, ensuring the effective activity of the siRNAs described in the present study This work offers an insight into the use of RNAi in animal ... fibroblastic, growing in a monolayer, and having a well-defined tendency for parallel orientation Viral infection causes a marked CPE resulting in total cellular detachment, rounding, and destruction, ... injected in the neck with 50–100 ug of plasmids dissolved in 100 ul of saline Mice of the control group were subcutaneously injected with saline After h, the suckling mice were challenged with and...

Ngày tải lên: 20/06/2014, 01:20

... Effects of active immunization of sheep against an amino terminal peptide of the inhibin αC subunit on intrafollicular levels of activin A, inhibin A and follistatin Journal of Endocrinology ... in mediating the effects of FSH on inhibin-A, activin-A, oestradiol and progesterone secretion and also important in mediating IGF-I stimulated inhibin-A, activin-A, follistatin, oestradiol and ... for luteinizing hormone and its interaction with insulin: activation of Janus kinase/signal transducer and activator of transcription and phosphoinositol 3-kinase/Akt pathways Endocrinology 2003,...

Ngày tải lên: 20/06/2014, 07:20

... Dilemmas in the reliable estimation of the in- vitro cell viability in magnetic nanoparticle engineering: which tests and what protocols? Clare Hoskins1, Lijun Wang*1, Woei Ping Cheng2 and Alfred ... endosomal magnetic labelling and in vivo tracking of stem cells Nat Biotechnol 2001, 19:1141-1147 Syková E, Jendelová P: Migration, fate and in vivo imaging of adult stem cells in the CNS Cell Death ... In the field of tissue engineering, magnetic nanoparticles have been previously reported for many applications including cellular labelling, sorting and monitoring, targeted in- vivo therapeutic...

Ngày tải lên: 20/06/2014, 20:20

... (s.c.) injections in the flank with × 106 cells in 0.2 mL of media containing 20% Matrigel ™(Becton Dickinson, Bedford, MA, USA) or intraperitoneal (i.p.) injections of × 10 cells in mL of media ... radiolabelings TN performed PET imaging and analysis MWB synthesized the chelate and participated in the design of the studies DEM conceived of and coordinated the studies and assisted with the in vivo ... containing Mg+2 and Ca+2 was added to each well of a 96-well plate Following an overnight incubation at 4°C, the solution was removed and 1% bovine serum albumin (BSA) in phosphate-buffered saline...

Ngày tải lên: 21/06/2014, 02:20

... were suspended in 100 μL of culture medium and subcutaneously inoculated into the right flank of mice using a 1.0 mL syringe Intravenous injection of reagents and tumor growth inhibition study ... and fixed in 100% methanol for 10 Cell monolayers were blocked in 5% BSA in PBS for 45 and incubated for h at room temperature with P-gp antibodies (Invitrogen, Beijing, China), followed by incubation ... culture and development of multidrug resistance Human hepatoma HepG2 cells were purchased from the Institute of Hematology of Tianjin, Chinese Academy of Medical Sciences (Tianjin, China) To...

Ngày tải lên: 21/06/2014, 03:20

... endosomal magnetic labelling and in vivo tracking of stem cells Nat Biotechnol 2001, 19:1141-1147 Syková E, Jendelová P: Migration, fate and in vivo imaging of adult stem cells in the CNS Cell Death ... a number of factors including an increase in the coating diameter upon addition of the PEG moiety [33], thus increasing the stability of the nanoparticles and further shielding the cells from ... foetal bovine serum [FBS], mM Lglutamine and 1% penicillin streptomycin [Penstrep] (all purchased from Invitrogen Ltd., Renfrew, UK) RAW264.7 cells (kindly donated by Prof Colin Watts and Dr Alan...

Ngày tải lên: 21/06/2014, 17:20

... Immunofluorescence and TUNEL staining in vivo Free-floating sections (40 µm) were used for all in vivo experiments for TUNEL staining and combined immunofluorescence staining Detection of nuclear ... provide rapid in vitro screens for analyzing new therapies and discovering means of achieving selective protection or targeted killing In light of the ease of use of these in vitro and in vivo assays, ... ethanol and a decreasing series of ethanol in 90%, 70%, and 50% for each, followed by rinsing in distilled water After three rinses in TBS, the sections were exposed to To combine TUNEL staining...

Ngày tải lên: 06/08/2014, 18:21

... ’rejuvenation’ which was expressed as improved shoot production and rooting in vitro and subsequently of micropropagated plants in the field Similar increased rooting ability of cuttings from micropropagated ... phloroglucinol in the proliferation medium improved subsequent rooting at the 11th and l5th subcultures but not at the 21 st subculture plantlets in compost following in vitro rooting but was increased ... micropropagated stockplants of the plum rootstock Pixy has been sustained for at least yr (Howard et aL, 1989a) Differences in shoot production and rooting of M.9 in vitro also appeared to originate from differences...

Ngày tải lên: 09/08/2014, 02:21

... chondrocytes in vitro and in situ Protein expression of COX-1 and COX-2 was studied using isoform-specific antibodies Both COX isoforms could be detected in all zones of the growth plate In cultured ... EP1, EP2, EP3, and EP4 in the growth plate and compared this profile with their expression in cultured growth plate chondrocytes in order to give innovative insights into in situ -in vitro correlations ... treatment of rheumatoid arthritis However, inadequate information is available on in situ expression of both COX-1 and COX-2 within the growth plate to correlate in vitro findings with the in situ...

Ngày tải lên: 09/08/2014, 08:22

... cells in vitro was tested in vivo We administered amiloride in vivo and showed reduction in the magnitude of CII-specific, but not polyclonal, T cell responses in draining lymph nodes, suggesting ... blockade of macropinocytosis prevented intracellular accumulation of CII and resulted in profound blockade of antigen presentation to T cells The involvement of macropinocytosis in uptake of autoantigens, ... data and error bars denoting 95% confidence interval are presented effect of amiloride and cytochalasin D was independent of NFκB inhibition Dose-response data obtained in the absence of inhibitors...

Ngày tải lên: 09/08/2014, 08:22